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The map label for this gene is folD-2

Identifier: 39995968

GI number: 39995968

Start: 920953

End: 921810

Strand: Reverse

Name: folD-2

Synonym: GSU0862

Alternate gene names: 39995968

Gene position: 921810-920953 (Counterclockwise)

Preceding gene: 39995969

Following gene: 308513290

Centisome position: 24.17

GC content: 62.7

Gene sequence:

>858_bases
ATGGCCACAATCATCGACGGAAAAGCCATTGCCGAAAAGATCCGGGCGGACCTCGCCGCCCAGGTGAAAGAACTGCAGTC
CAGGGGAATAACCCCCGGCCTCGCCACGGTCCTGGTGGGGAGCGACCCAGCCAGCGAAGTCTACGTCCGGATGAAGGGCG
ATGCCTGCAATAAGCTCGGCATGCACTCGGTGAAGATCACGCGCCCCGCGGAAACGACCGAGGAGGAGCTCCTCGCGCTC
ATCAACGAACTCAATAACGATCCGGCGATCCACGGCATCCTCGTGCAGCTCCCCCTTCCGCCGCAGATCAACGCGGATCG
TGTCCTGGAAGCCATCTCACCCGCCAAGGACGTGGACGGCTTCCATCCTTACAATGTGGGACGCCTGGTGACCGGCAAGC
CGACCTTCCAGCCCTGCACCCCCTACGGCGTAATGGTGATGCTGCAAGAAGCGGGAGTCGACTTGGCCGGCAAGGAAGTG
GTGGTAGTGGGACGCTCAAACATCGTGGGCAAGCCAGTGGCACTGATGTGCCTTCAGCGCAACGCCACCGTCACCATCTG
TCACTCGAAGACCCGCGACCTCCCCGGCCGGGTCAGGGCCGCCGACGTGGTCATCGCCGCAGTCGGGGTCCCCGAGATGA
TCAAAGGGGACTGGATTAAGGAAGGAGCGGTTGTCATCGACGTGGGCGTCAACCGGGTGGGAGAAAAGAAACTTGTGGGA
GACGTGGAGTTCGCCGCAGCAGCCGAACGGGCTTCGGCCATCACGCCGGTCCCCGGCGGGGTGGGCCCCATGACCATCAC
CATGCTTCTTCACAACACCCTTGAGGCCGCAAAAATGGCGGGTTCCGGGAATCGCTAG

Upstream 100 bases:

>100_bases
CGGTAATCTGACTTTACAACAAGGGCCCCTTTCCGTATCATTCTGTGGCTGTTCTCACCATTTTTCCCTGCCGGGACGCA
CATCCAGAAGGAGCCCACCC

Downstream 100 bases:

>100_bases
CCTGGCACCGGAATCACGATGATAGTCAGCAGGCAGAAACCCTTCGCCGATATCCTGGCCGCCTTGGACGGCGTCTCGCG
GGTCTTCGTGATCGGCTGCT

Product: folD bifunctional protein

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MATIIDGKAIAEKIRADLAAQVKELQSRGITPGLATVLVGSDPASEVYVRMKGDACNKLGMHSVKITRPAETTEEELLAL
INELNNDPAIHGILVQLPLPPQINADRVLEAISPAKDVDGFHPYNVGRLVTGKPTFQPCTPYGVMVMLQEAGVDLAGKEV
VVVGRSNIVGKPVALMCLQRNATVTICHSKTRDLPGRVRAADVVIAAVGVPEMIKGDWIKEGAVVIDVGVNRVGEKKLVG
DVEFAAAAERASAITPVPGGVGPMTITMLLHNTLEAAKMAGSGNR

Sequences:

>Translated_285_residues
MATIIDGKAIAEKIRADLAAQVKELQSRGITPGLATVLVGSDPASEVYVRMKGDACNKLGMHSVKITRPAETTEEELLAL
INELNNDPAIHGILVQLPLPPQINADRVLEAISPAKDVDGFHPYNVGRLVTGKPTFQPCTPYGVMVMLQEAGVDLAGKEV
VVVGRSNIVGKPVALMCLQRNATVTICHSKTRDLPGRVRAADVVIAAVGVPEMIKGDWIKEGAVVIDVGVNRVGEKKLVG
DVEFAAAAERASAITPVPGGVGPMTITMLLHNTLEAAKMAGSGNR
>Mature_284_residues
ATIIDGKAIAEKIRADLAAQVKELQSRGITPGLATVLVGSDPASEVYVRMKGDACNKLGMHSVKITRPAETTEEELLALI
NELNNDPAIHGILVQLPLPPQINADRVLEAISPAKDVDGFHPYNVGRLVTGKPTFQPCTPYGVMVMLQEAGVDLAGKEVV
VVGRSNIVGKPVALMCLQRNATVTICHSKTRDLPGRVRAADVVIAAVGVPEMIKGDWIKEGAVVIDVGVNRVGEKKLVGD
VEFAAAAERASAITPVPGGVGPMTITMLLHNTLEAAKMAGSGNR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=47.7508650519031, Blast_Score=257, Evalue=8e-69,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=44.3686006825939, Blast_Score=224, Evalue=5e-59,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=46.7576791808874, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI36796743, Length=202, Percent_Identity=30.6930693069307, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=53.4296028880866, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=298, Percent_Identity=42.2818791946309, Blast_Score=210, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=50.1754385964912, Blast_Score=272, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=46.0750853242321, Blast_Score=254, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6322933, Length=321, Percent_Identity=27.1028037383178, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=47.7351916376307, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=47.7351916376307, Blast_Score=256, Evalue=9e-69,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=47.7351916376307, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=47.7351916376307, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=47.2789115646259, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=47.2789115646259, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD2_GEOSL (Q74EU7)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951919.1
- HSSP:   P11586
- ProteinModelPortal:   Q74EU7
- SMR:   Q74EU7
- GeneID:   2687021
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0862
- NMPDR:   fig|243231.1.peg.857
- TIGR:   GSU0862
- HOGENOM:   HBG328751
- OMA:   LLEHYHI
- ProtClustDB:   CLSK828063
- BioCyc:   GSUL243231:GSU_0862-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 29999; Mature: 29868

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIIDGKAIAEKIRADLAAQVKELQSRGITPGLATVLVGSDPASEVYVRMKGDACNKLG
CCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCC
MHSVKITRPAETTEEELLALINELNNDPAIHGILVQLPLPPQINADRVLEAISPAKDVDG
CCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHCCHHHCCC
FHPYNVGRLVTGKPTFQPCTPYGVMVMLQEAGVDLAGKEVVVVGRSNIVGKPVALMCLQR
CCCCCCCEEEECCCCCCCCCCHHEEEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
NATVTICHSKTRDLPGRVRAADVVIAAVGVPEMIKGDWIKEGAVVIDVGVNRVGEKKLVG
CCEEEEEECCCCCCCCCEEHHHEEEEECCCCHHHCCCCCCCCCEEEEECCCCCCCHHHCC
DVEFAAAAERASAITPVPGGVGPMTITMLLHNTLEAAKMAGSGNR
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATIIDGKAIAEKIRADLAAQVKELQSRGITPGLATVLVGSDPASEVYVRMKGDACNKLG
CEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCC
MHSVKITRPAETTEEELLALINELNNDPAIHGILVQLPLPPQINADRVLEAISPAKDVDG
CCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHCCHHHCCC
FHPYNVGRLVTGKPTFQPCTPYGVMVMLQEAGVDLAGKEVVVVGRSNIVGKPVALMCLQR
CCCCCCCEEEECCCCCCCCCCHHEEEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
NATVTICHSKTRDLPGRVRAADVVIAAVGVPEMIKGDWIKEGAVVIDVGVNRVGEKKLVG
CCEEEEEECCCCCCCCCEEHHHEEEEECCCCHHHCCCCCCCCCEEEEECCCCCCCHHHCC
DVEFAAAAERASAITPVPGGVGPMTITMLLHNTLEAAKMAGSGNR
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA