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The map label for this gene is pleD [H]

Identifier: 39995914

GI number: 39995914

Start: 866291

End: 867253

Strand: Reverse

Name: pleD [H]

Synonym: GSU0808

Alternate gene names: 39995914

Gene position: 867253-866291 (Counterclockwise)

Preceding gene: 39995915

Following gene: 39995913

Centisome position: 22.74

GC content: 67.81

Gene sequence:

>963_bases
ATGTCCAGGAACCACCTGCCGTTCAGCGCCGGCGAACTGGAAGCGCTGCCCCTGTTCCGCTTCGTTCCCCTCGAGTGCAT
CGAAGGCATTCTTGAGCACAGTTGCGTGGTCAGCCTGGAGCGGGGCGAGCGCTTTGAGCCGGCCGGCCCTCTGGGTCGAA
CCATCCACCTGATCCTGGACGGCCGCCTCGCCCTCTACTACGACGTCCGGGAACGGGACGACACGCCCCTGCACCTGGAG
CGGGGCGACATCGTCGGTCAAAGCCTCGTTTCCGGTGAGCCGGAAGTCCCCTGCACCCTCGTGGCCGCTGAAGAAACGCG
GCTCATGATCCTGGAGGAGGACCTGATCTGGTCCCTGGCCCAGGCATCCCACGCAGCCGCCTGCAACCTGCTCGGCATCC
TTCTGCGCGGCAGGGGAGGCTCTCCCCAGGAGAGCTTCTCGGGGACCGTGCGCGACTACGCCCTCCGCAACCAGGCGGTG
GTGGACCCCCTTACCGGTTTCCAGAGCAGACGTTGGCTGGAGGGGATTCTCTCGCGGCAGGTGGATCGCTTCACCGCCAA
CGGCCGGCCCCTGTCCCTGGTGATGATCGACATCGATGGCTTTCGCGCGTTCAACGAGCGCCACGGTCGCGCCGGCGGCG
ACCGGGCCCTGCACGACGTCGCCAAAACCCTCCGGAACTCCCTCCGCCCCGGCGAGCTGGTGGCGCGCTACGGCGGCGAC
ACCTTCGCGGTGCTCCTCCCCGAAACGGAACCATCCACAGCATGGATGATCGCGGACCGGCTCAGGCAACGCCTGATGCG
CACCGTCATCGACATGGGGGACGGCGCCATGCTGCCGCCGCTCTCCATTTCGGCGGGGGTGGCCGAAGCCACCCCGGGCA
TCGACGGACCCACCATTGCGGATAACGCGCTCGCGGCGCTCGACAGGGCAAAGGCAGCGGGGGGCAACACCGTTTCCCGT
TAG

Upstream 100 bases:

>100_bases
CCGCGGGATGTACCGGTCCACGGCCTCATCTTCTGCCCCAACGACGGCCATCTTGAAGTAATCGTCAGGGGATACTGATC
CCCCGGTTCGCGAGGATCCC

Downstream 100 bases:

>100_bases
CCTCCCGACCGGATCGTTCCTTCTCGCAATTTTGTTCTGTGACCGGAGCCGGCAGCGGTCACAAGGGCAGACATTGCGGA
ACGTGCCAGTCAGCCGGACC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSRNHLPFSAGELEALPLFRFVPLECIEGILEHSCVVSLERGERFEPAGPLGRTIHLILDGRLALYYDVRERDDTPLHLE
RGDIVGQSLVSGEPEVPCTLVAAEETRLMILEEDLIWSLAQASHAAACNLLGILLRGRGGSPQESFSGTVRDYALRNQAV
VDPLTGFQSRRWLEGILSRQVDRFTANGRPLSLVMIDIDGFRAFNERHGRAGGDRALHDVAKTLRNSLRPGELVARYGGD
TFAVLLPETEPSTAWMIADRLRQRLMRTVIDMGDGAMLPPLSISAGVAEATPGIDGPTIADNALAALDRAKAAGGNTVSR

Sequences:

>Translated_320_residues
MSRNHLPFSAGELEALPLFRFVPLECIEGILEHSCVVSLERGERFEPAGPLGRTIHLILDGRLALYYDVRERDDTPLHLE
RGDIVGQSLVSGEPEVPCTLVAAEETRLMILEEDLIWSLAQASHAAACNLLGILLRGRGGSPQESFSGTVRDYALRNQAV
VDPLTGFQSRRWLEGILSRQVDRFTANGRPLSLVMIDIDGFRAFNERHGRAGGDRALHDVAKTLRNSLRPGELVARYGGD
TFAVLLPETEPSTAWMIADRLRQRLMRTVIDMGDGAMLPPLSISAGVAEATPGIDGPTIADNALAALDRAKAAGGNTVSR
>Mature_319_residues
SRNHLPFSAGELEALPLFRFVPLECIEGILEHSCVVSLERGERFEPAGPLGRTIHLILDGRLALYYDVRERDDTPLHLER
GDIVGQSLVSGEPEVPCTLVAAEETRLMILEEDLIWSLAQASHAAACNLLGILLRGRGGSPQESFSGTVRDYALRNQAVV
DPLTGFQSRRWLEGILSRQVDRFTANGRPLSLVMIDIDGFRAFNERHGRAGGDRALHDVAKTLRNSLRPGELVARYGGDT
FAVLLPETEPSTAWMIADRLRQRLMRTVIDMGDGAMLPPLSISAGVAEATPGIDGPTIADNALAALDRAKAAGGNTVSR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=32.7272727272727, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=34.3373493975904, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=31.7365269461078, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=28.9156626506024, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786584, Length=156, Percent_Identity=32.0512820512821, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082007, Length=131, Percent_Identity=33.587786259542, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=108, Percent_Identity=26.8518518518519, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=31.2101910828025, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=35, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34808; Mature: 34677

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNHLPFSAGELEALPLFRFVPLECIEGILEHSCVVSLERGERFEPAGPLGRTIHLILD
CCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEEE
GRLALYYDVRERDDTPLHLERGDIVGQSLVSGEPEVPCTLVAAEETRLMILEEDLIWSLA
CCEEEEEEECCCCCCCEEEECCCHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHHH
QASHAAACNLLGILLRGRGGSPQESFSGTVRDYALRNQAVVDPLTGFQSRRWLEGILSRQ
HHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCHHCHHHCHHHHHHHHHHHHHH
VDRFTANGRPLSLVMIDIDGFRAFNERHGRAGGDRALHDVAKTLRNSLRPGELVARYGGD
HHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCC
TFAVLLPETEPSTAWMIADRLRQRLMRTVIDMGDGAMLPPLSISAGVAEATPGIDGPTIA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCH
DNALAALDRAKAAGGNTVSR
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRNHLPFSAGELEALPLFRFVPLECIEGILEHSCVVSLERGERFEPAGPLGRTIHLILD
CCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEEE
GRLALYYDVRERDDTPLHLERGDIVGQSLVSGEPEVPCTLVAAEETRLMILEEDLIWSLA
CCEEEEEEECCCCCCCEEEECCCHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHHH
QASHAAACNLLGILLRGRGGSPQESFSGTVRDYALRNQAVVDPLTGFQSRRWLEGILSRQ
HHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCHHCHHHCHHHHHHHHHHHHHH
VDRFTANGRPLSLVMIDIDGFRAFNERHGRAGGDRALHDVAKTLRNSLRPGELVARYGGD
HHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCC
TFAVLLPETEPSTAWMIADRLRQRLMRTVIDMGDGAMLPPLSISAGVAEATPGIDGPTIA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCH
DNALAALDRAKAAGGNTVSR
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA