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The map label for this gene is ppsA [H]

Identifier: 39995909

GI number: 39995909

Start: 861123

End: 863540

Strand: Reverse

Name: ppsA [H]

Synonym: GSU0803

Alternate gene names: 39995909

Gene position: 863540-861123 (Counterclockwise)

Preceding gene: 39995910

Following gene: 39995908

Centisome position: 22.64

GC content: 65.92

Gene sequence:

>2418_bases
ATGTCCTGGATTCGCTGGTTCTCGGAAATAACCATTGATGACGTCCCCCTGGTCGGGGGCAAGAACGCCTCCCTCGGCGA
AATGTTCGGGGAGCTCAGGCCTCTGGGGGTGCGGATTCCCAACGGCTTCGCAATCACGGCGGAGGCCTACTGGCACCTGA
TCCGGTCGGCGGGTATCCTGGAAGAAATGAAACAGGCCCTGGCGGAACTGGTCAAGGAGGACGTGGATGACTTGGCGCTC
CGGGGGCGCCGGCTGCGGGAGCTGGTGTACGGCGCCCCCCTCCCGGGGGAATTGGCGGAAGAGATCAGGGAGGCCTACCG
GGGGCTCTGCGCCGAGTATGGCGATAACTGCGACGTGGCGGTCCGCAGCTCGGCCACGGCAGAAGATCTCCCCACCGCCT
CCTTCGCGGGGCAGCAGGAGACCTACCTGAACGTCCGGGGCGAGGCGCAGCTCCTGGACGCCTGCCGCCGCTGCTTCGCC
TCCCTCTTCACCGACCGTGCCATTTCATACCGCATCGACCAGGGGTTCGACCACTTCAAGGTGGGGCTCTCCGTGGGCGT
CATGAAGATGGTCCGGTCGGATCTCGCCTCCAGCGGGGTCATCTTCACCCTAGACACCGAGACCGGCTTCCGGGACACGG
TTTTGATCACCGGCGCCTGGGGGCTGGGCGAGAACGTGGTCCAGGGGGCCGTGAACCCGGACGAGTTCACGGTTTTCAAG
CCCGCGCTCCGGCAGGGATTCCGCCCCATCGTCCGGCGGCGGCTGGGGGAGAAGAAAATCCGGATGATCTACGGCACCGG
CACCTCCAAGCACCTGACCAAGAACGTGGAGGTGCCCCGGGAGGAGCGGCGCCGCTTCTGCCTCAGCGACGACGAGGTGC
TGGAGTTGGCCCGCCAGGCCCTCATGATCGAGTCGCACTATGAGAAGCGGGCCGGCCGCGAGATGCCCATGGACATCGAA
TGGGCCAAGGACGGGGAGTCGGGCCGGCTCTTCATCGTCCAGGCCCGGCCCGAGACGGTGGAATCCCGCAAGCGGCTCGA
CTTCCTGGAGACCTACATCCTGGAGCAGAAGGGAGAGGTGCTGGCCACCGGTACCAGCGTGGGCGGAAAGATCGCCGCCG
GCCCGGCCCGGGTCATCACCGACATCCACAAGCTGGGCGACTTCAGCCCCGGCGAGGTCCTGGTGGCCGATACCACCACC
CCCGACTGGGAGCCGGTCATGAAGACCGCCGCGGCCATCGTCACCAACAAGGGGGGGCGCACCTGCCACGCCGCCATCGT
CAGTCGCGAGCTGGGCATCCCGTCGGTGGTCGGCACCGGCGACGGGACCGAGCGGATCGCCAGCAACCGGGAGATAACGG
TGAGCTGCGCCGAAGGGGAAGTCGGCAGAATCTACAGCGGCGAACTCCCCTTCCGGGTGGAGCGGGTTGACCTGGCCGGC
ATGCGGCGCCCCCACACCAGGATCATGATGAACCTGGGCAATCCGGAGGAAGCCTTCTCCCTCTGCCGGATTCCCAACGA
CGGGGTGGGGCTCGCCCGGATGGAGTTCATCATCACCAACCACATCAAGATCCATCCCATGGCCCTGGTTCACCCCCTCC
GGGTGGAGGACGAGGCATCGCGGCGCGAGATCGCGCGCCTCACCGCCGGCTATGCCGACCCGCCCGCCTACTTTGTGGAA
AAGCTTGCCGAGGGGATCGGCACCATTGCCGCCGCCTTCCATCCCAAACCGGTCATTGTCCGCATGAGCGACTTCAAGAC
CAACGAGTACGCCTCGCTCCTGGGGGGCCGGGCCTTCGAGCCCGACGAGGAGAACCCCATGATCGGCTTCCGGGGGGCGT
CGCGCTACTACGACGAGCGCTACCGGGAAGGATTCGCCCTGGAGTGCCGGGCCCTGAAGCGGGTCCGGGACGAGATGGGA
CTCGTGAACGTAATCCCCATGATCCCCTTCTGCCGACGCATCGTGGAGGCGGAGAAGGTACTGGCCGAAATGGCGGCAAA
CGGCCTCGTCCGGGGCGAAAACGGCCTGGAAGTCTACGTCATGTGCGAGATCCCCAACAACGTCATCATGATCGACGAGT
TCTCGCGGCTTTTCGACGGTTTTTCCATCGGCTCCAACGACCTGACCCAGCTCACCCTCGGCGTGGACCGGGACTCGGCC
CTGGTGGCCCACGTCTTTGACGAGCGGGACCCGGGGGTCATGGAACTCATCGGCCGGGTCGTGGAGGGGGCGCGCCGCAA
CGGACGCCACAGCGGCATCTGCGGCCAGGCCCCCAGTGACTACCCCGAGTTCGCGGCCTTTCTCGTGGAGCAGGGAATCG
ACTCCATTTCGCTCAACCCCGATTCGATCCTCAAGATCACGATGAGGGTCCTGGAAATGGAGGAGCAGCTGGCGCAGCAG
GGGAACAAGAGGAGTTAG

Upstream 100 bases:

>100_bases
GCCGAGCGAGAACGATCTGGCCGGGGCCCGCTTCCAGGGCAGGTATGTGGCGCAGATCGCCGCCAAACTGAACGGATAGG
AAGCACAGGGAGGTTCTTCC

Downstream 100 bases:

>100_bases
ACATGTCGCTCAACGATGCAAAGGTAGTAGTGATCGGCGGCAGTTCCGGCATGGGGCTCGCCGTGGCGAAGATGGCCGCG
GACGAGGGGGCCCGGGTGGT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSWIRWFSEITIDDVPLVGGKNASLGEMFGELRPLGVRIPNGFAITAEAYWHLIRSAGILEEMKQALAELVKEDVDDLAL
RGRRLRELVYGAPLPGELAEEIREAYRGLCAEYGDNCDVAVRSSATAEDLPTASFAGQQETYLNVRGEAQLLDACRRCFA
SLFTDRAISYRIDQGFDHFKVGLSVGVMKMVRSDLASSGVIFTLDTETGFRDTVLITGAWGLGENVVQGAVNPDEFTVFK
PALRQGFRPIVRRRLGEKKIRMIYGTGTSKHLTKNVEVPREERRRFCLSDDEVLELARQALMIESHYEKRAGREMPMDIE
WAKDGESGRLFIVQARPETVESRKRLDFLETYILEQKGEVLATGTSVGGKIAAGPARVITDIHKLGDFSPGEVLVADTTT
PDWEPVMKTAAAIVTNKGGRTCHAAIVSRELGIPSVVGTGDGTERIASNREITVSCAEGEVGRIYSGELPFRVERVDLAG
MRRPHTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKIHPMALVHPLRVEDEASRREIARLTAGYADPPAYFVE
KLAEGIGTIAAAFHPKPVIVRMSDFKTNEYASLLGGRAFEPDEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMG
LVNVIPMIPFCRRIVEAEKVLAEMAANGLVRGENGLEVYVMCEIPNNVIMIDEFSRLFDGFSIGSNDLTQLTLGVDRDSA
LVAHVFDERDPGVMELIGRVVEGARRNGRHSGICGQAPSDYPEFAAFLVEQGIDSISLNPDSILKITMRVLEMEEQLAQQ
GNKRS

Sequences:

>Translated_805_residues
MSWIRWFSEITIDDVPLVGGKNASLGEMFGELRPLGVRIPNGFAITAEAYWHLIRSAGILEEMKQALAELVKEDVDDLAL
RGRRLRELVYGAPLPGELAEEIREAYRGLCAEYGDNCDVAVRSSATAEDLPTASFAGQQETYLNVRGEAQLLDACRRCFA
SLFTDRAISYRIDQGFDHFKVGLSVGVMKMVRSDLASSGVIFTLDTETGFRDTVLITGAWGLGENVVQGAVNPDEFTVFK
PALRQGFRPIVRRRLGEKKIRMIYGTGTSKHLTKNVEVPREERRRFCLSDDEVLELARQALMIESHYEKRAGREMPMDIE
WAKDGESGRLFIVQARPETVESRKRLDFLETYILEQKGEVLATGTSVGGKIAAGPARVITDIHKLGDFSPGEVLVADTTT
PDWEPVMKTAAAIVTNKGGRTCHAAIVSRELGIPSVVGTGDGTERIASNREITVSCAEGEVGRIYSGELPFRVERVDLAG
MRRPHTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKIHPMALVHPLRVEDEASRREIARLTAGYADPPAYFVE
KLAEGIGTIAAAFHPKPVIVRMSDFKTNEYASLLGGRAFEPDEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMG
LVNVIPMIPFCRRIVEAEKVLAEMAANGLVRGENGLEVYVMCEIPNNVIMIDEFSRLFDGFSIGSNDLTQLTLGVDRDSA
LVAHVFDERDPGVMELIGRVVEGARRNGRHSGICGQAPSDYPEFAAFLVEQGIDSISLNPDSILKITMRVLEMEEQLAQQ
GNKRS
>Mature_804_residues
SWIRWFSEITIDDVPLVGGKNASLGEMFGELRPLGVRIPNGFAITAEAYWHLIRSAGILEEMKQALAELVKEDVDDLALR
GRRLRELVYGAPLPGELAEEIREAYRGLCAEYGDNCDVAVRSSATAEDLPTASFAGQQETYLNVRGEAQLLDACRRCFAS
LFTDRAISYRIDQGFDHFKVGLSVGVMKMVRSDLASSGVIFTLDTETGFRDTVLITGAWGLGENVVQGAVNPDEFTVFKP
ALRQGFRPIVRRRLGEKKIRMIYGTGTSKHLTKNVEVPREERRRFCLSDDEVLELARQALMIESHYEKRAGREMPMDIEW
AKDGESGRLFIVQARPETVESRKRLDFLETYILEQKGEVLATGTSVGGKIAAGPARVITDIHKLGDFSPGEVLVADTTTP
DWEPVMKTAAAIVTNKGGRTCHAAIVSRELGIPSVVGTGDGTERIASNREITVSCAEGEVGRIYSGELPFRVERVDLAGM
RRPHTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKIHPMALVHPLRVEDEASRREIARLTAGYADPPAYFVEK
LAEGIGTIAAAFHPKPVIVRMSDFKTNEYASLLGGRAFEPDEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMGL
VNVIPMIPFCRRIVEAEKVLAEMAANGLVRGENGLEVYVMCEIPNNVIMIDEFSRLFDGFSIGSNDLTQLTLGVDRDSAL
VAHVFDERDPGVMELIGRVVEGARRNGRHSGICGQAPSDYPEFAAFLVEQGIDSISLNPDSILKITMRVLEMEEQLAQQG
NKRS

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=796, Percent_Identity=53.1407035175879, Blast_Score=855, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=417, Percent_Identity=26.378896882494, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789193, Length=423, Percent_Identity=27.1867612293144, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994992, Length=395, Percent_Identity=25.5696202531646, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788726, Length=388, Percent_Identity=27.0618556701031, Blast_Score=93, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17564524, Length=233, Percent_Identity=30.9012875536481, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 89287; Mature: 89156

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWIRWFSEITIDDVPLVGGKNASLGEMFGELRPLGVRIPNGFAITAEAYWHLIRSAGIL
CCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHCHHH
EEMKQALAELVKEDVDDLALRGRRLRELVYGAPLPGELAEEIREAYRGLCAEYGDNCDVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VRSSATAEDLPTASFAGQQETYLNVRGEAQLLDACRRCFASLFTDRAISYRIDQGFDHFK
EECCCCHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHH
VGLSVGVMKMVRSDLASSGVIFTLDTETGFRDTVLITGAWGLGENVVQGAVNPDEFTVFK
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEH
PALRQGFRPIVRRRLGEKKIRMIYGTGTSKHLTKNVEVPREERRRFCLSDDEVLELARQA
HHHHHCHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHH
LMIESHYEKRAGREMPMDIEWAKDGESGRLFIVQARPETVESRKRLDFLETYILEQKGEV
HHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCE
LATGTSVGGKIAAGPARVITDIHKLGDFSPGEVLVADTTTPDWEPVMKTAAAIVTNKGGR
EEECCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
TCHAAIVSRELGIPSVVGTGDGTERIASNREITVSCAEGEVGRIYSGELPFRVERVDLAG
EEHHHHHHHHCCCCCEEECCCCHHHHCCCCCEEEEECCCCCCCEECCCCCEEEEEECCCC
MRRPHTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKIHPMALVHPLRVEDEAS
CCCCCCEEEEECCCHHHHHHHHCCCCCCCCHHHEEEEEECCEEEEEHHHCCCCCCCCHHH
RREIARLTAGYADPPAYFVEKLAEGIGTIAAAFHPKPVIVRMSDFKTNEYASLLGGRAFE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCC
PDEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMGLVNVIPMIPFCRRIVEAEKV
CCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAEMAANGLVRGENGLEVYVMCEIPNNVIMIDEFSRLFDGFSIGSNDLTQLTLGVDRDSA
HHHHHHCCCEECCCCCEEEEEEECCCCEEEEECHHHHHCCCCCCCCCCEEEEEECCCCCH
LVAHVFDERDPGVMELIGRVVEGARRNGRHSGICGQAPSDYPEFAAFLVEQGIDSISLNP
HHEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
DSILKITMRVLEMEEQLAQQGNKRS
HHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SWIRWFSEITIDDVPLVGGKNASLGEMFGELRPLGVRIPNGFAITAEAYWHLIRSAGIL
CHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCEEEEHHHHHHHHHHCHHH
EEMKQALAELVKEDVDDLALRGRRLRELVYGAPLPGELAEEIREAYRGLCAEYGDNCDVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VRSSATAEDLPTASFAGQQETYLNVRGEAQLLDACRRCFASLFTDRAISYRIDQGFDHFK
EECCCCHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHH
VGLSVGVMKMVRSDLASSGVIFTLDTETGFRDTVLITGAWGLGENVVQGAVNPDEFTVFK
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEH
PALRQGFRPIVRRRLGEKKIRMIYGTGTSKHLTKNVEVPREERRRFCLSDDEVLELARQA
HHHHHCHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHH
LMIESHYEKRAGREMPMDIEWAKDGESGRLFIVQARPETVESRKRLDFLETYILEQKGEV
HHHHHHHHHHCCCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCE
LATGTSVGGKIAAGPARVITDIHKLGDFSPGEVLVADTTTPDWEPVMKTAAAIVTNKGGR
EEECCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
TCHAAIVSRELGIPSVVGTGDGTERIASNREITVSCAEGEVGRIYSGELPFRVERVDLAG
EEHHHHHHHHCCCCCEEECCCCHHHHCCCCCEEEEECCCCCCCEECCCCCEEEEEECCCC
MRRPHTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKIHPMALVHPLRVEDEAS
CCCCCCEEEEECCCHHHHHHHHCCCCCCCCHHHEEEEEECCEEEEEHHHCCCCCCCCHHH
RREIARLTAGYADPPAYFVEKLAEGIGTIAAAFHPKPVIVRMSDFKTNEYASLLGGRAFE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCC
PDEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMGLVNVIPMIPFCRRIVEAEKV
CCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAEMAANGLVRGENGLEVYVMCEIPNNVIMIDEFSRLFDGFSIGSNDLTQLTLGVDRDSA
HHHHHHCCCEECCCCCEEEEEEECCCCEEEEECHHHHHCCCCCCCCCCEEEEEECCCCCH
LVAHVFDERDPGVMELIGRVVEGARRNGRHSGICGQAPSDYPEFAAFLVEQGIDSISLNP
HHEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
DSILKITMRVLEMEEQLAQQGNKRS
HHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]