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The map label for this gene is pilJ [H]

Identifier: 39995689

GI number: 39995689

Start: 613603

End: 615693

Strand: Direct

Name: pilJ [H]

Synonym: GSU0582

Alternate gene names: 39995689

Gene position: 613603-615693 (Clockwise)

Preceding gene: 39995688

Following gene: 39995702

Centisome position: 16.09

GC content: 61.84

Gene sequence:

>2091_bases
ATGTTTCGAACGAGACTTGCCTTCAAGGTTCTCGCCATTATCGGTATAACTCTGTTTTTAGGGTTTGCCGCCCTCGGAAT
CACCTCTATCTGGCTTGAATACAACGCAATCATGGATCTCCAGACCCGCAACACCCGCGGGCTCTCGACCCTGGTGGTCC
GTGACATCGGCGAACTCATGATGGCGGGTGACATGGCCGTCATTGAGCGCTACGTGGCCGACGTTCGAGGCAAGGGTGCC
GTTCTCGACCTGCGCATCTATGATGCCGCGGGAAGGCCGGCCGGCAAAAAGCAGGATGCGCCCGACGGAGAGGTGCAGGC
GGCCCTGACCTCCGGGGCCACTGCGGAAAAGCGTCACAAGGTGGACGGGCGGCACGTGCTGAGCTTTATCGTTCCCCTGG
CCAATGAGGTGCGCTGTCAGTCGTGCCATGAGCAGGGCGCCCGCTTCAATGGCGCCATGCTTCTCACAACGTCGCTGGAG
GAAGGATACGCCGGCGCCCGGAACCTGACCCTGGCCCTGGCCCTGGTGGGCGTATGCTCGTTCTTTCTCCTCCTTGGCGT
GATGTACCTGTTTTTCAATCGGGTCATCATCAGGAATATCGGTGAGATATCGAGACGGGTGCAGGAAATCGCCCAGGGTG
AGGGGGACCTTACCGCCTCCGTGCCGGTGCGTTCAAGCGACGAACTCGGGGTTCTGGCCGAGGGAATCAACCTTCTTGTA
ACCAAGCTCAGGGAGATTATCTCGGGCCTGTATCACCAGGCCGGACATATCGCCATTTCCGCCTGCCGCACCATCAAGGA
AACCGAGCGGCTGGTGGCCTCCACCCACGAGCAGAAGGATCTGTCCACGTCAGTGGCGGTTGCCTCCGAGGAGATGGCGG
CAACACTCAACGATGTCGCCGTCAATACCCAGCGGGCAGCCCAGCTGTCCCTTTCCGTGGACCGGGCCGCCCATGAGGGG
ATGGCCACCGTGACGGAGACCGCCGAGAGTATCGACCGGATCAAGGACAGCGTCATGGCAACGCTCGACACCATGGACAA
GCTGCAGCAGTCGTCGGGCCAGATCGGTGAGATTGTCGGCATCATCGGGGACATCGCCGACCAGACCAACCTCCTGGCGC
TCAACGCGGCAATAGAAGCGGCCCGGGCGGGAGACTCGGGCAAGGGATTCGCGGTGGTGGCCAATGAGGTGAAGGTACTG
TCGGACCGGACTGCCTCCTCCACCCGGGAAATCGGGACCATCATCAGGAGCATTCAGGCGGAGATCCGCGCGGTGGTGGC
ATCCATTGCCGAAGGGAAGGACAAGGTCGAGGTGGGCGTCGAACGGTCAACTACGGCGCGGCGGCAGCTGGAGGATATCC
TGCGCCTGGCGGCTGAGTCCACGGACATGATCAACCAGATCGCCACGGCAACAGAGGAGCAGAGCGCCACCACCGGCGAG
ATATCCGAAAAGATATCCCAGGTTTCGGGCACGGCTGAACGGGTAAACGGCCAGATGGAGCAGACGGCGGGAATCTTCCG
GGAGCTCTCGGAGACGGCCGAGCAGATTTACGGCACCGTGGGGCGGTTCAAGGTGGGGACCTATCACGATACGGTCAAAG
GGCTGGCGTCGGAGATGCGCGACCGGGTCGTCGCGACGCTTGAGCGGGCCGCGTCGGATCGGCGCGTTACTCTTGATGCG
CTGTTCAGCAGCGAGTACACGCCGATCCCCGACACCTTCCCCCAGAAATACCGCACGCCCTCAGACCGGCTCTTCGACGA
GATTATTTCACCGATCCAGGAGGAGATCCTGGGGCGCGACAGCGGCATGTACTACGCCATCTGCGTTGACCGGCGCGGTT
ACTGTCCTTCCCATAACCTCCGCTATTCGCGCCCTCTTACCGGCAACCGGGAGGCGGACAAGGAGCATAACCGGACCAAA
CGGATTTTTGAGGATCGTACGGGGCTGCGGTGCGCGGGCAATACGGGGAGTTTCCTGCTGCAGACCTACCTGCGGGACAC
GGGCGAGGTGATGAATGACCTTTCAGTGCCGATTGTTATCGGCGGCAGGCACTGGGGTGCCGTGAGGATCGGCTACCGGG
CCGACGATTGA

Upstream 100 bases:

>100_bases
TATTCATCTTGCTAAAACAATTTTATAAATTGTTAAAGGTCTGGGGAGGCCTGCCGATACAATAAGATAAGTACACGCCA
CGGTCAGAAGGAGCCCCGTC

Downstream 100 bases:

>100_bases
GGCCGGCTCCGGTTACCGGACCTTGAAGCCGGCCATGGCCTCGGTGAGCCCTTCTGTCTGGCGCGCAAGTCGGGAGACGG
CCTCTTCCATGAGCCGGGTC

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MFRTRLAFKVLAIIGITLFLGFAALGITSIWLEYNAIMDLQTRNTRGLSTLVVRDIGELMMAGDMAVIERYVADVRGKGA
VLDLRIYDAAGRPAGKKQDAPDGEVQAALTSGATAEKRHKVDGRHVLSFIVPLANEVRCQSCHEQGARFNGAMLLTTSLE
EGYAGARNLTLALALVGVCSFFLLLGVMYLFFNRVIIRNIGEISRRVQEIAQGEGDLTASVPVRSSDELGVLAEGINLLV
TKLREIISGLYHQAGHIAISACRTIKETERLVASTHEQKDLSTSVAVASEEMAATLNDVAVNTQRAAQLSLSVDRAAHEG
MATVTETAESIDRIKDSVMATLDTMDKLQQSSGQIGEIVGIIGDIADQTNLLALNAAIEAARAGDSGKGFAVVANEVKVL
SDRTASSTREIGTIIRSIQAEIRAVVASIAEGKDKVEVGVERSTTARRQLEDILRLAAESTDMINQIATATEEQSATTGE
ISEKISQVSGTAERVNGQMEQTAGIFRELSETAEQIYGTVGRFKVGTYHDTVKGLASEMRDRVVATLERAASDRRVTLDA
LFSSEYTPIPDTFPQKYRTPSDRLFDEIISPIQEEILGRDSGMYYAICVDRRGYCPSHNLRYSRPLTGNREADKEHNRTK
RIFEDRTGLRCAGNTGSFLLQTYLRDTGEVMNDLSVPIVIGGRHWGAVRIGYRADD

Sequences:

>Translated_696_residues
MFRTRLAFKVLAIIGITLFLGFAALGITSIWLEYNAIMDLQTRNTRGLSTLVVRDIGELMMAGDMAVIERYVADVRGKGA
VLDLRIYDAAGRPAGKKQDAPDGEVQAALTSGATAEKRHKVDGRHVLSFIVPLANEVRCQSCHEQGARFNGAMLLTTSLE
EGYAGARNLTLALALVGVCSFFLLLGVMYLFFNRVIIRNIGEISRRVQEIAQGEGDLTASVPVRSSDELGVLAEGINLLV
TKLREIISGLYHQAGHIAISACRTIKETERLVASTHEQKDLSTSVAVASEEMAATLNDVAVNTQRAAQLSLSVDRAAHEG
MATVTETAESIDRIKDSVMATLDTMDKLQQSSGQIGEIVGIIGDIADQTNLLALNAAIEAARAGDSGKGFAVVANEVKVL
SDRTASSTREIGTIIRSIQAEIRAVVASIAEGKDKVEVGVERSTTARRQLEDILRLAAESTDMINQIATATEEQSATTGE
ISEKISQVSGTAERVNGQMEQTAGIFRELSETAEQIYGTVGRFKVGTYHDTVKGLASEMRDRVVATLERAASDRRVTLDA
LFSSEYTPIPDTFPQKYRTPSDRLFDEIISPIQEEILGRDSGMYYAICVDRRGYCPSHNLRYSRPLTGNREADKEHNRTK
RIFEDRTGLRCAGNTGSFLLQTYLRDTGEVMNDLSVPIVIGGRHWGAVRIGYRADD
>Mature_696_residues
MFRTRLAFKVLAIIGITLFLGFAALGITSIWLEYNAIMDLQTRNTRGLSTLVVRDIGELMMAGDMAVIERYVADVRGKGA
VLDLRIYDAAGRPAGKKQDAPDGEVQAALTSGATAEKRHKVDGRHVLSFIVPLANEVRCQSCHEQGARFNGAMLLTTSLE
EGYAGARNLTLALALVGVCSFFLLLGVMYLFFNRVIIRNIGEISRRVQEIAQGEGDLTASVPVRSSDELGVLAEGINLLV
TKLREIISGLYHQAGHIAISACRTIKETERLVASTHEQKDLSTSVAVASEEMAATLNDVAVNTQRAAQLSLSVDRAAHEG
MATVTETAESIDRIKDSVMATLDTMDKLQQSSGQIGEIVGIIGDIADQTNLLALNAAIEAARAGDSGKGFAVVANEVKVL
SDRTASSTREIGTIIRSIQAEIRAVVASIAEGKDKVEVGVERSTTARRQLEDILRLAAESTDMINQIATATEEQSATTGE
ISEKISQVSGTAERVNGQMEQTAGIFRELSETAEQIYGTVGRFKVGTYHDTVKGLASEMRDRVVATLERAASDRRVTLDA
LFSSEYTPIPDTFPQKYRTPSDRLFDEIISPIQEEILGRDSGMYYAICVDRRGYCPSHNLRYSRPLTGNREADKEHNRTK
RIFEDRTGLRCAGNTGSFLLQTYLRDTGEVMNDLSVPIVIGGRHWGAVRIGYRADD

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=355, Percent_Identity=27.0422535211268, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI2367378, Length=302, Percent_Identity=31.1258278145695, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789453, Length=328, Percent_Identity=28.0487804878049, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI1788195, Length=303, Percent_Identity=30.3630363036304, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788194, Length=376, Percent_Identity=25.7978723404255, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 75774; Mature: 75774

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRTRLAFKVLAIIGITLFLGFAALGITSIWLEYNAIMDLQTRNTRGLSTLVVRDIGELM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHH
MAGDMAVIERYVADVRGKGAVLDLRIYDAAGRPAGKKQDAPDGEVQAALTSGATAEKRHK
HHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHC
VDGRHVLSFIVPLANEVRCQSCHEQGARFNGAMLLTTSLEEGYAGARNLTLALALVGVCS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHH
FFLLLGVMYLFFNRVIIRNIGEISRRVQEIAQGEGDLTASVPVRSSDELGVLAEGINLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHH
TKLREIISGLYHQAGHIAISACRTIKETERLVASTHEQKDLSTSVAVASEEMAATLNDVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNTQRAAQLSLSVDRAAHEGMATVTETAESIDRIKDSVMATLDTMDKLQQSSGQIGEIVG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IIGDIADQTNLLALNAAIEAARAGDSGKGFAVVANEVKVLSDRTASSTREIGTIIRSIQA
HHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHHH
EIRAVVASIAEGKDKVEVGVERSTTARRQLEDILRLAAESTDMINQIATATEEQSATTGE
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ISEKISQVSGTAERVNGQMEQTAGIFRELSETAEQIYGTVGRFKVGTYHDTVKGLASEMR
HHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHH
DRVVATLERAASDRRVTLDALFSSEYTPIPDTFPQKYRTPSDRLFDEIISPIQEEILGRD
HHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCC
SGMYYAICVDRRGYCPSHNLRYSRPLTGNREADKEHNRTKRIFEDRTGLRCAGNTGSFLL
CCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHHH
QTYLRDTGEVMNDLSVPIVIGGRHWGAVRIGYRADD
HHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCC
>Mature Secondary Structure
MFRTRLAFKVLAIIGITLFLGFAALGITSIWLEYNAIMDLQTRNTRGLSTLVVRDIGELM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHH
MAGDMAVIERYVADVRGKGAVLDLRIYDAAGRPAGKKQDAPDGEVQAALTSGATAEKRHK
HHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHC
VDGRHVLSFIVPLANEVRCQSCHEQGARFNGAMLLTTSLEEGYAGARNLTLALALVGVCS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHH
FFLLLGVMYLFFNRVIIRNIGEISRRVQEIAQGEGDLTASVPVRSSDELGVLAEGINLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHH
TKLREIISGLYHQAGHIAISACRTIKETERLVASTHEQKDLSTSVAVASEEMAATLNDVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNTQRAAQLSLSVDRAAHEGMATVTETAESIDRIKDSVMATLDTMDKLQQSSGQIGEIVG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IIGDIADQTNLLALNAAIEAARAGDSGKGFAVVANEVKVLSDRTASSTREIGTIIRSIQA
HHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHHH
EIRAVVASIAEGKDKVEVGVERSTTARRQLEDILRLAAESTDMINQIATATEEQSATTGE
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ISEKISQVSGTAERVNGQMEQTAGIFRELSETAEQIYGTVGRFKVGTYHDTVKGLASEMR
HHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHH
DRVVATLERAASDRRVTLDALFSSEYTPIPDTFPQKYRTPSDRLFDEIISPIQEEILGRD
HHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCC
SGMYYAICVDRRGYCPSHNLRYSRPLTGNREADKEHNRTKRIFEDRTGLRCAGNTGSFLL
CCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHHH
QTYLRDTGEVMNDLSVPIVIGGRHWGAVRIGYRADD
HHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]