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The map label for this gene is folA [H]

Identifier: 39995678

GI number: 39995678

Start: 599582

End: 600064

Strand: Direct

Name: folA [H]

Synonym: GSU0571

Alternate gene names: 39995678

Gene position: 599582-600064 (Clockwise)

Preceding gene: 39995677

Following gene: 308513283

Centisome position: 15.72

GC content: 69.36

Gene sequence:

>483_bases
ATGCGCATCACCCTTATCGCGGCCATGGCCGAAAACCGCGTCATCGGCAACCGGGGGGCCATCCCCTGGCACCTGCCGGA
CGACCTGGCCCGCTTCAGGGCCATCACCCTGGGGCACCCGGTCCTCATGGGGCGCAGGACCTTCGAGTCCATCGGTCGGC
CGCTGCCGGGGCGGCTGAACATCGTCCTTTCCCATCGGGAAGGCTATGCCCCGCCGGGGTGCTTGGTGGCCCGGGACCTG
GCCGCGGCGCTGGAGCTGGCCCGGGACGCGGCGGAGCTCTTTGTCTGCGGCGGCGGCGAGCTCTACCGGGAAGCGCTGCC
CCTGGCCGACCGGATTCTTCTCACTCTGGTGCAGGGGGAATTTCCCGGCGACGTCACCTTTCCGGAGGTTCCGGCTGATT
TCGTGGAAACGGCGCGGGAGGAGGGGGTAGGGGGGCCGGCCCACCTGTTCCTGACCTATGAGCGGAGGAAAGAGGCCCCA
TGA

Upstream 100 bases:

>100_bases
CCCGGGAAATCGAGCGGGACGTGGTGGAAGGGCGGCTCCACACGGGCCGGGGCGCCGTGGTGCAGATCGTGGCGCGCAAG
CCGTGAGGGGGCCGGATTCC

Downstream 100 bases:

>100_bases
GCACCGAACCACGCAGGTTCTCCGTCTTCCTCGTCCCCGCGGCCGGGGACCGCCGGTGGGCGGATGGAGTGATCCACGAG
CTGGCCGACCGCTACGATAC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRITLIAAMAENRVIGNRGAIPWHLPDDLARFRAITLGHPVLMGRRTFESIGRPLPGRLNIVLSHREGYAPPGCLVARDL
AAALELARDAAELFVCGGGELYREALPLADRILLTLVQGEFPGDVTFPEVPADFVETAREEGVGGPAHLFLTYERRKEAP

Sequences:

>Translated_160_residues
MRITLIAAMAENRVIGNRGAIPWHLPDDLARFRAITLGHPVLMGRRTFESIGRPLPGRLNIVLSHREGYAPPGCLVARDL
AAALELARDAAELFVCGGGELYREALPLADRILLTLVQGEFPGDVTFPEVPADFVETAREEGVGGPAHLFLTYERRKEAP
>Mature_160_residues
MRITLIAAMAENRVIGNRGAIPWHLPDDLARFRAITLGHPVLMGRRTFESIGRPLPGRLNIVLSHREGYAPPGCLVARDL
AAALELARDAAELFVCGGGELYREALPLADRILLTLVQGEFPGDVTFPEVPADFVETAREEGVGGPAHLFLTYERRKEAP

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=186, Percent_Identity=29.5698924731183, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=42.7672955974843, Blast_Score=121, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17505899, Length=163, Percent_Identity=33.1288343558282, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324810, Length=136, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI116008026, Length=155, Percent_Identity=34.8387096774194, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647458, Length=155, Percent_Identity=34.8387096774194, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17491; Mature: 17491

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITLIAAMAENRVIGNRGAIPWHLPDDLARFRAITLGHPVLMGRRTFESIGRPLPGRLN
CEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCCEEE
IVLSHREGYAPPGCLVARDLAAALELARDAAELFVCGGGELYREALPLADRILLTLVQGE
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCHHHHHHHHHHCCC
FPGDVTFPEVPADFVETAREEGVGGPAHLFLTYERRKEAP
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEHHCCCCC
>Mature Secondary Structure
MRITLIAAMAENRVIGNRGAIPWHLPDDLARFRAITLGHPVLMGRRTFESIGRPLPGRLN
CEEEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHCCCCCCEEE
IVLSHREGYAPPGCLVARDLAAALELARDAAELFVCGGGELYREALPLADRILLTLVQGE
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCHHHHHHHHHHCCC
FPGDVTFPEVPADFVETAREEGVGGPAHLFLTYERRKEAP
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]