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The map label for this gene is prfA [H]

Identifier: 39995653

GI number: 39995653

Start: 578710

End: 579090

Strand: Direct

Name: prfA [H]

Synonym: GSU0546

Alternate gene names: 39995653

Gene position: 578710-579090 (Clockwise)

Preceding gene: 39995652

Following gene: 39995654

Centisome position: 15.17

GC content: 64.83

Gene sequence:

>381_bases
GTGGCGCCACCGACCTTTGCCGTGAGTGAAGAGAAAAACCGCTGGCTCCGGGACAAGATGGCCGAACTGGGGGTGAGCGA
GGCGGACCTGGAGGAAACCTTTGTCCGCTCCTCCGGCGCCGGCGGCCAGCACGTGAACAAGACCGCCACCTGCGTCCAGA
TCCGGCATCGCCCCACCGGCATCGAAGTGAAGTGCATGAAGGACCGGAGCCAGTCCGTGAACCGCTTTCTGGCCCGGCGC
GAGATCCTGGAGCGGATCGAACGGCTCAACAAGGGGGAGAGCCCCCGGGACGTCCGGGTCGAGAAGCTCCGGAAGCAGAA
GGCGCGGCGGAAACGGCGGGCCACCACGAAATACGACCCAGAGAAGGACAATCAGCAATGA

Upstream 100 bases:

>100_bases
TCATCCTCATGGCCGTGGCCGGGCCGCTCCTGGCCCGGCTCTATCTGGAGCAGCGCCATCGGCGGCTCTACCGGCTCGAT
GGCGCCGAAGGGACCTAGCC

Downstream 100 bases:

>100_bases
TCAGAACCGAAACCCTCCGTACCCTCGAATTCGATAAAATACTTTCGGCCGTGTCGGGCTACGCCCACAGCGGCGCCACG
CGGGACGAAACCGCCCTGAT

Product: peptidyl-tRNA hydrolase domain-containing protein

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MAPPTFAVSEEKNRWLRDKMAELGVSEADLEETFVRSSGAGGQHVNKTATCVQIRHRPTGIEVKCMKDRSQSVNRFLARR
EILERIERLNKGESPRDVRVEKLRKQKARRKRRATTKYDPEKDNQQ

Sequences:

>Translated_126_residues
MAPPTFAVSEEKNRWLRDKMAELGVSEADLEETFVRSSGAGGQHVNKTATCVQIRHRPTGIEVKCMKDRSQSVNRFLARR
EILERIERLNKGESPRDVRVEKLRKQKARRKRRATTKYDPEKDNQQ
>Mature_125_residues
APPTFAVSEEKNRWLRDKMAELGVSEADLEETFVRSSGAGGQHVNKTATCVQIRHRPTGIEVKCMKDRSQSVNRFLARRE
ILERIERLNKGESPRDVRVEKLRKQKARRKRRATTKYDPEKDNQQ

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI303304971, Length=112, Percent_Identity=41.0714285714286, Blast_Score=77, Evalue=5e-15,
Organism=Homo sapiens, GI219879802, Length=112, Percent_Identity=41.0714285714286, Blast_Score=77, Evalue=5e-15,
Organism=Homo sapiens, GI22748627, Length=112, Percent_Identity=41.0714285714286, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI2367172, Length=72, Percent_Identity=41.6666666666667, Blast_Score=62, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24654113, Length=91, Percent_Identity=46.1538461538462, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 14663; Mature: 14532

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPPTFAVSEEKNRWLRDKMAELGVSEADLEETFVRSSGAGGQHVNKTATCVQIRHRPTG
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEECCCCC
IEVKCMKDRSQSVNRFLARREILERIERLNKGESPRDVRVEKLRKQKARRKRRATTKYDP
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EKDNQQ
CCCCCC
>Mature Secondary Structure 
APPTFAVSEEKNRWLRDKMAELGVSEADLEETFVRSSGAGGQHVNKTATCVQIRHRPTG
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEECCCCC
IEVKCMKDRSQSVNRFLARREILERIERLNKGESPRDVRVEKLRKQKARRKRRATTKYDP
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
EKDNQQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA