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The map label for this gene is macA [H]

Identifier: 39995604

GI number: 39995604

Start: 528747

End: 529955

Strand: Direct

Name: macA [H]

Synonym: GSU0496

Alternate gene names: 39995604

Gene position: 528747-529955 (Clockwise)

Preceding gene: 39995603

Following gene: 39995605

Centisome position: 13.86

GC content: 62.45

Gene sequence:

>1209_bases
ATGAAAAAAGCAGCCATCATCGCGGCGGTTGTCATCGTAGCCGTAGCGGGAATCTATTTCGCCTTCGGCAAGCGTGAACC
CGAACGGACCTACAAGACTGCAAAAGTGGAACGGGGCGATGTCGTTGCCACCGTCTCCGCCACGGGCAACCTGAGCGCCG
TCACTACCGTTCAGGTCGGGACCCAGGTCTCCGGCACCATCTATCGCCTGCTCGTGGACTACAATACCCCCGTGAAAAAG
GGACAGGTCATCGCCGAGATCGATCCGGCACTGTTTCGGGCCGACGTGGAGCAGGCAAGGGGCAACCTTCTGAGCGCGCA
GGCGAATCTCCTGAAAGCCAGGGCCACTGCCGCCGATGCCAAGCGGACCATGGAGCGCAACCGGCAACTGGTCAAGGAGG
GAGTTGTCTCCCAGAGCGACTTCGACACCGCCGAAACCGGTGCCCAAGAGGCCGCCGCCGCCGTCAAGGCCGCTGAAGCC
TCGGTCGTCCAGACCCGGGGTGCCCTGTCCCGGGCGGAAACCAACCTGAAGAACGCCACCATCCGGTCGCCGGTGGATGG
TGTCGTCATATCCCGGGCCGTGGACGTGGGACAGACCGTGGCCGCCTCCTTCCAGACCCCGACCCTGTTCACCATCGCCC
AGGATCTCACCCGGATGCAGATCGAGACCAGCGTTGACGAGGCCGACATCAGCCGCGTGCGGATGGGACAGCAGGTTACC
TTCACCGTGGATGCCTACCCGGACGAGAAGTTCAATGCCCGGGTGGCCCAGATCCGCAATGCCCCGGTCATTACCCAGAA
TGTGGTCACCTACGTGGTGGTTATCACCGTTGACAACCCCGACTTGAAGCTCAAGCCGGGCATGACTGCCAATGTGGCCA
TTGAGGTGGGCAAAAAGGAAAAAGTGCTCAAAGTTCCGATGGCTGCACTGCGGTTCAAGCCCCGCGCGGACGGTGACGAG
GGTGAGCGCCGCGCTCCGTCCGGTCCGGCAAAGGCGAAGCGGGAGGCGGGACAGCAGGTCTTCATCCTGTCACCGGAGAA
GAAGCCGGTGCCGGTGAAGGTACAGACCGGTCTCGGCAACGACAGCCATGTGGAGATCGTCTCCGGAGCCTTGAAGGAGG
GAGACGAGGTGATCGTTCAGGAGACCACTCCCAGGGATAAACAGAAGAGCGGCGGCAGCGGCAGATCTCCCATGGGGATG
CGGTTCTAG

Upstream 100 bases:

>100_bases
CCTGTCGTTTGCGGCAGGGGGCTTTTTCATGTCAACGGTTGGTGCGTTCTGCCGTTTTTGATATATAATCCCCGTGCCAT
CTTTCCCCCATGAGGTGAGA

Downstream 100 bases:

>100_bases
ATGGGCGCGGTCGTCAGGCTCGATAACGTCGCCAAGGTCTACACCATGGGGGACCAGCGGGTGGAGGCCCTGCGGGGCAT
ATCCTTCTCGGTGGAGCAGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKKAAIIAAVVIVAVAGIYFAFGKREPERTYKTAKVERGDVVATVSATGNLSAVTTVQVGTQVSGTIYRLLVDYNTPVKK
GQVIAEIDPALFRADVEQARGNLLSAQANLLKARATAADAKRTMERNRQLVKEGVVSQSDFDTAETGAQEAAAAVKAAEA
SVVQTRGALSRAETNLKNATIRSPVDGVVISRAVDVGQTVAASFQTPTLFTIAQDLTRMQIETSVDEADISRVRMGQQVT
FTVDAYPDEKFNARVAQIRNAPVITQNVVTYVVVITVDNPDLKLKPGMTANVAIEVGKKEKVLKVPMAALRFKPRADGDE
GERRAPSGPAKAKREAGQQVFILSPEKKPVPVKVQTGLGNDSHVEIVSGALKEGDEVIVQETTPRDKQKSGGSGRSPMGM
RF

Sequences:

>Translated_402_residues
MKKAAIIAAVVIVAVAGIYFAFGKREPERTYKTAKVERGDVVATVSATGNLSAVTTVQVGTQVSGTIYRLLVDYNTPVKK
GQVIAEIDPALFRADVEQARGNLLSAQANLLKARATAADAKRTMERNRQLVKEGVVSQSDFDTAETGAQEAAAAVKAAEA
SVVQTRGALSRAETNLKNATIRSPVDGVVISRAVDVGQTVAASFQTPTLFTIAQDLTRMQIETSVDEADISRVRMGQQVT
FTVDAYPDEKFNARVAQIRNAPVITQNVVTYVVVITVDNPDLKLKPGMTANVAIEVGKKEKVLKVPMAALRFKPRADGDE
GERRAPSGPAKAKREAGQQVFILSPEKKPVPVKVQTGLGNDSHVEIVSGALKEGDEVIVQETTPRDKQKSGGSGRSPMGM
RF
>Mature_402_residues
MKKAAIIAAVVIVAVAGIYFAFGKREPERTYKTAKVERGDVVATVSATGNLSAVTTVQVGTQVSGTIYRLLVDYNTPVKK
GQVIAEIDPALFRADVEQARGNLLSAQANLLKARATAADAKRTMERNRQLVKEGVVSQSDFDTAETGAQEAAAAVKAAEA
SVVQTRGALSRAETNLKNATIRSPVDGVVISRAVDVGQTVAASFQTPTLFTIAQDLTRMQIETSVDEADISRVRMGQQVT
FTVDAYPDEKFNARVAQIRNAPVITQNVVTYVVVITVDNPDLKLKPGMTANVAIEVGKKEKVLKVPMAALRFKPRADGDE
GERRAPSGPAKAKREAGQQVFILSPEKKPVPVKVQTGLGNDSHVEIVSGALKEGDEVIVQETTPRDKQKSGGSGRSPMGM
RF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=384, Percent_Identity=31.7708333333333, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87082036, Length=342, Percent_Identity=27.1929824561404, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789929, Length=259, Percent_Identity=29.3436293436293, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790519, Length=241, Percent_Identity=30.7053941908714, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787013, Length=296, Percent_Identity=23.6486486486486, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789041, Length=279, Percent_Identity=26.1648745519713, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42984; Mature: 42984

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAAIIAAVVIVAVAGIYFAFGKREPERTYKTAKVERGDVVATVSATGNLSAVTTVQVG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCEEEEEECCCCCEEEEEEEEC
TQVSGTIYRLLVDYNTPVKKGQVIAEIDPALFRADVEQARGNLLSAQANLLKARATAADA
CCCCCEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
KRTMERNRQLVKEGVVSQSDFDTAETGAQEAAAAVKAAEASVVQTRGALSRAETNLKNAT
HHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IRSPVDGVVISRAVDVGQTVAASFQTPTLFTIAQDLTRMQIETSVDEADISRVRMGQQVT
ECCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
FTVDAYPDEKFNARVAQIRNAPVITQNVVTYVVVITVDNPDLKLKPGMTANVAIEVGKKE
EEEECCCCCCCCHHHHHHCCCCCEEECEEEEEEEEEECCCCEEECCCCEEEEEEEECCCC
KVLKVPMAALRFKPRADGDEGERRAPSGPAKAKREAGQQVFILSPEKKPVPVKVQTGLGN
CHHHCCHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECCCC
DSHVEIVSGALKEGDEVIVQETTPRDKQKSGGSGRSPMGMRF
CCCHHHHHHHHCCCCEEEEECCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKAAIIAAVVIVAVAGIYFAFGKREPERTYKTAKVERGDVVATVSATGNLSAVTTVQVG
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCEEEEEECCCCCEEEEEEEEC
TQVSGTIYRLLVDYNTPVKKGQVIAEIDPALFRADVEQARGNLLSAQANLLKARATAADA
CCCCCEEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
KRTMERNRQLVKEGVVSQSDFDTAETGAQEAAAAVKAAEASVVQTRGALSRAETNLKNAT
HHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IRSPVDGVVISRAVDVGQTVAASFQTPTLFTIAQDLTRMQIETSVDEADISRVRMGQQVT
ECCCCCCHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
FTVDAYPDEKFNARVAQIRNAPVITQNVVTYVVVITVDNPDLKLKPGMTANVAIEVGKKE
EEEECCCCCCCCHHHHHHCCCCCEEECEEEEEEEEEECCCCEEECCCCEEEEEEEECCCC
KVLKVPMAALRFKPRADGDEGERRAPSGPAKAKREAGQQVFILSPEKKPVPVKVQTGLGN
CHHHCCHHHHEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECCCC
DSHVEIVSGALKEGDEVIVQETTPRDKQKSGGSGRSPMGMRF
CCCHHHHHHHHCCCCEEEEECCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]