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The map label for this gene is hycF [C]

Identifier: 39995602

GI number: 39995602

Start: 527317

End: 528447

Strand: Direct

Name: hycF [C]

Synonym: GSU0494

Alternate gene names: 39995602

Gene position: 527317-528447 (Clockwise)

Preceding gene: 39995601

Following gene: 39995603

Centisome position: 13.83

GC content: 65.69

Gene sequence:

>1131_bases
ATGCACACGGTAGCCGTCGAACGGGCCGCACGCTACGATCCTTCAGAGGTTGCCGAAGCCATGGACCGGGCCCTCGCTTC
CCTGGGAGGAATGGATACCTTTGTCCGTCCTGGTGAGCGGGTTCTGATCAAGCCGAACATGCTGGCTGCCAAGGCACCCG
AGCGGGCGGTGACCACCCACCCGGAGGTGCTGCGGGCCGTGATTCGGCTCGTCCGGAAGGCCGGCGGAATCCCGCTGGTG
GGCGATTCTCCCGGTATCGGCGGCTTTCGGGCCGTGGCGGAAAAGAGCGGCATGGCTGCCGTCGTCCGGGAGGCCGGTGC
CGAACTGGTCCCGTTTGACGAGGCTGTTGCCGTTCCGGGCAGCGGGCTCTTCAGGCGTATGGATGTGGCGCGACCCTACC
TGGAAGCCGATCGGCTCATCAACCTTCCCAAGCTCAAGACCCACGAGATGATGACCATGACCTGCGCCGTGAAGAATCTT
TTCGGCGCAGTGGTCGGGACCGCCAAGGCGGGCTGGCATCTCAAGGCCGGCGCCGACCGGGAGCTGTTTGCCCGGCTTCT
CCTGGAAATCTACCTTCTGAGGCCACCGGATCTGACCATCGTTGACGGCATTGTGGCCATGGAGGGTGATGGTCCGGGCA
GCGGAGACCCCAGGCCGATGGGGCTCATTCTGGCTGGAGCCAATGCCGTGGCGGTGGATGTGGTAGCCGCGGAACTGGCG
GGTATTCCGAAGCAGCTCCTCTGGGTCGAGCGTGCCGCCGAACGGCTCGGCATCGACGGCTGGGACCGGTCCCGCATCGC
GACGGTCGGACTCCCGCCCGACGATGCCCGGGTGCCGGACTTTCGCTTGCCGCACCTGTCGGACGTCCAGTTCGGCATCC
CCGGTTTCCTCAAGAACCGGCTTCGTCATCATCTCACTGCGCGGCCGGTGCCGAACCCCGAGGGGTGCCACCTGTGCGGG
GCGTGCCTTGACGCCTGTCCTCCACGGGCCATCTCCGTGCGCGACGGGCGTCTCCATTTCGACTACCACGCCTGCATCCG
CTGCTTCTGCTGCCGCGAACTCTGCCCCGACGGCTCCCTCGGGGTACGGGATGGAGTGCTGCTGAAACTGCTCAAGAAAT
TCAAGGAGTAA

Upstream 100 bases:

>100_bases
TTCGCAACCGTATTGTGGTCATGCTCAACTGGATCTGGTACTACTGGTTCCATGAGCGCCAGGTGCGCCTCATCACGGAG
CGTGAAACACCGGGCAACGG

Downstream 100 bases:

>100_bases
ACTTTAATGGCTCCGCTCCTTGACGTTGCGGGGGGGCAGGATTATCATAACTATAACCTCGCGCACTGAGGAAATCCCCC
TGAAAGGAGACCCCAGGCCA

Product: iron-sulfur cluster-binding protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MHTVAVERAARYDPSEVAEAMDRALASLGGMDTFVRPGERVLIKPNMLAAKAPERAVTTHPEVLRAVIRLVRKAGGIPLV
GDSPGIGGFRAVAEKSGMAAVVREAGAELVPFDEAVAVPGSGLFRRMDVARPYLEADRLINLPKLKTHEMMTMTCAVKNL
FGAVVGTAKAGWHLKAGADRELFARLLLEIYLLRPPDLTIVDGIVAMEGDGPGSGDPRPMGLILAGANAVAVDVVAAELA
GIPKQLLWVERAAERLGIDGWDRSRIATVGLPPDDARVPDFRLPHLSDVQFGIPGFLKNRLRHHLTARPVPNPEGCHLCG
ACLDACPPRAISVRDGRLHFDYHACIRCFCCRELCPDGSLGVRDGVLLKLLKKFKE

Sequences:

>Translated_376_residues
MHTVAVERAARYDPSEVAEAMDRALASLGGMDTFVRPGERVLIKPNMLAAKAPERAVTTHPEVLRAVIRLVRKAGGIPLV
GDSPGIGGFRAVAEKSGMAAVVREAGAELVPFDEAVAVPGSGLFRRMDVARPYLEADRLINLPKLKTHEMMTMTCAVKNL
FGAVVGTAKAGWHLKAGADRELFARLLLEIYLLRPPDLTIVDGIVAMEGDGPGSGDPRPMGLILAGANAVAVDVVAAELA
GIPKQLLWVERAAERLGIDGWDRSRIATVGLPPDDARVPDFRLPHLSDVQFGIPGFLKNRLRHHLTARPVPNPEGCHLCG
ACLDACPPRAISVRDGRLHFDYHACIRCFCCRELCPDGSLGVRDGVLLKLLKKFKE
>Mature_376_residues
MHTVAVERAARYDPSEVAEAMDRALASLGGMDTFVRPGERVLIKPNMLAAKAPERAVTTHPEVLRAVIRLVRKAGGIPLV
GDSPGIGGFRAVAEKSGMAAVVREAGAELVPFDEAVAVPGSGLFRRMDVARPYLEADRLINLPKLKTHEMMTMTCAVKNL
FGAVVGTAKAGWHLKAGADRELFARLLLEIYLLRPPDLTIVDGIVAMEGDGPGSGDPRPMGLILAGANAVAVDVVAAELA
GIPKQLLWVERAAERLGIDGWDRSRIATVGLPPDDARVPDFRLPHLSDVQFGIPGFLKNRLRHHLTARPVPNPEGCHLCG
ACLDACPPRAISVRDGRLHFDYHACIRCFCCRELCPDGSLGVRDGVLLKLLKKFKE

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG2006

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007160 [H]

Pfam domain/function: PF04015 DUF362 [H]

EC number: NA

Molecular weight: Translated: 40520; Mature: 40520

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTVAVERAARYDPSEVAEAMDRALASLGGMDTFVRPGERVLIKPNMLAAKAPERAVTTH
CCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHCCCCCCCCCCC
PEVLRAVIRLVRKAGGIPLVGDSPGIGGFRAVAEKSGMAAVVREAGAELVPFDEAVAVPG
HHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCEECCC
SGLFRRMDVARPYLEADRLINLPKLKTHEMMTMTCAVKNLFGAVVGTAKAGWHLKAGADR
CHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCH
ELFARLLLEIYLLRPPDLTIVDGIVAMEGDGPGSGDPRPMGLILAGANAVAVDVVAAELA
HHHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
GIPKQLLWVERAAERLGIDGWDRSRIATVGLPPDDARVPDFRLPHLSDVQFGIPGFLKNR
CCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LRHHLTARPVPNPEGCHLCGACLDACPPRAISVRDGRLHFDYHACIRCFCCRELCPDGSL
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCEEEECHHHHHHHHHHHHHCCCCCC
GVRDGVLLKLLKKFKE
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHTVAVERAARYDPSEVAEAMDRALASLGGMDTFVRPGERVLIKPNMLAAKAPERAVTTH
CCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHCCCCCCCCCCC
PEVLRAVIRLVRKAGGIPLVGDSPGIGGFRAVAEKSGMAAVVREAGAELVPFDEAVAVPG
HHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCEECCC
SGLFRRMDVARPYLEADRLINLPKLKTHEMMTMTCAVKNLFGAVVGTAKAGWHLKAGADR
CHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCH
ELFARLLLEIYLLRPPDLTIVDGIVAMEGDGPGSGDPRPMGLILAGANAVAVDVVAAELA
HHHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHH
GIPKQLLWVERAAERLGIDGWDRSRIATVGLPPDDARVPDFRLPHLSDVQFGIPGFLKNR
CCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LRHHLTARPVPNPEGCHLCGACLDACPPRAISVRDGRLHFDYHACIRCFCCRELCPDGSL
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCEEEECHHHHHHHHHHHHHCCCCCC
GVRDGVLLKLLKKFKE
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]