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The map label for this gene is fliM [H]

Identifier: 39995529

GI number: 39995529

Start: 451662

End: 452648

Strand: Direct

Name: fliM [H]

Synonym: GSU0421

Alternate gene names: 39995529

Gene position: 451662-452648 (Clockwise)

Preceding gene: 39995528

Following gene: 39995530

Centisome position: 11.84

GC content: 59.57

Gene sequence:

>987_bases
ATGGAGAAGATCCTCACCAAGCAAGAGATAGAGGCGCTGCTTGCCGCGGTATTCGAGGGGAAGATCGAACCCGACCGTGA
GCTGGCAAAGGAGGAGGGCACCGTCCACTCCTACGACCTCTTCAACAGCGAAGCCCACAAGGGGCTCGTTCCCAACCTGG
ACATCATTTACGACGGCTTCATCCGCTATCAGCGGGGGACCCTGTCGAACCGGCTGGGGCGCATCGTCGAGATCAAGAAG
CTCGGGGCCGGATCGTACAAGTTCGACGACTTCATCCAGACCCTCCCTTCACCGGTCTGCATGGCGATCTACAAGGCCGA
CCCGCTCAAGGGGGCGGCCCTGATCGCCTTTGACAGCACGCTCGTTTTTACCATCGTGGACTGCATTCTGGGCGGAACCG
GCATGACGTCGGTCCAAACGAGCGCCAACCGGATGTTCACCTCCATTGAGCTGCGGCTGGTCCAGAAGATCGTGCAGGAT
ATGCTCGTCGACCTGGAAAAGGCCTGGGCACCGCTCTATGCAGCCAAGATGAGCCTGCTCCGGATGGAGATGAACCCGCG
GCTCGTGAATATCGTTCCCCCTGAGTACCAGGTGGTAACGATGGAGATGCAGATCCAGATCGACCAGTTAGAGGGGAAGA
TGGTTTTTGCCGTCCCCTATATGACCATCGAGCCGATTCGGGACAAGCTCAAGTCCGGCGCCCAGTTCGACCTCATGGCC
ATTGATCCCCAGTGGTCATTCCGGCTGTCCAAGGAACTGCTGGAGGCTCCCCTGGACGTATCGGTGGAGGTCGGCGGTGC
GGTCATCAGCCTCAACGACCTGCTCAGCCTGGTCCCCGGTGACACCATCATGCTTGATACCCCCTGCACCAGCGATCTCA
CGGTCAAGGTCGGCGGGGTGCCCAAATTCACGGGAATGCCGGGCATACGCCACGGCAACAAGGCACTTCAGATCACGAAC
GTCGTAGGCAAGGGAGAACAGCGGTGA

Upstream 100 bases:

>100_bases
GCCAACAAGATTCTGCCTCCGGGCAAGGTAACCAAGGTCTATTTCACCGATTTCGTGGTCCAGTAGCTGACAGGACGTCA
AGGGAGGCAGAGGAATCTCC

Downstream 100 bases:

>100_bases
GCGACTTCACGAAAGAAGAAACCAAAGACGGTGAACTGGACCGCAAGAACCTGGAGTTCATTCTCGATATACCGCTTCAG
CTCACGGTGGAACTGGGCCG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MEKILTKQEIEALLAAVFEGKIEPDRELAKEEGTVHSYDLFNSEAHKGLVPNLDIIYDGFIRYQRGTLSNRLGRIVEIKK
LGAGSYKFDDFIQTLPSPVCMAIYKADPLKGAALIAFDSTLVFTIVDCILGGTGMTSVQTSANRMFTSIELRLVQKIVQD
MLVDLEKAWAPLYAAKMSLLRMEMNPRLVNIVPPEYQVVTMEMQIQIDQLEGKMVFAVPYMTIEPIRDKLKSGAQFDLMA
IDPQWSFRLSKELLEAPLDVSVEVGGAVISLNDLLSLVPGDTIMLDTPCTSDLTVKVGGVPKFTGMPGIRHGNKALQITN
VVGKGEQR

Sequences:

>Translated_328_residues
MEKILTKQEIEALLAAVFEGKIEPDRELAKEEGTVHSYDLFNSEAHKGLVPNLDIIYDGFIRYQRGTLSNRLGRIVEIKK
LGAGSYKFDDFIQTLPSPVCMAIYKADPLKGAALIAFDSTLVFTIVDCILGGTGMTSVQTSANRMFTSIELRLVQKIVQD
MLVDLEKAWAPLYAAKMSLLRMEMNPRLVNIVPPEYQVVTMEMQIQIDQLEGKMVFAVPYMTIEPIRDKLKSGAQFDLMA
IDPQWSFRLSKELLEAPLDVSVEVGGAVISLNDLLSLVPGDTIMLDTPCTSDLTVKVGGVPKFTGMPGIRHGNKALQITN
VVGKGEQR
>Mature_328_residues
MEKILTKQEIEALLAAVFEGKIEPDRELAKEEGTVHSYDLFNSEAHKGLVPNLDIIYDGFIRYQRGTLSNRLGRIVEIKK
LGAGSYKFDDFIQTLPSPVCMAIYKADPLKGAALIAFDSTLVFTIVDCILGGTGMTSVQTSANRMFTSIELRLVQKIVQD
MLVDLEKAWAPLYAAKMSLLRMEMNPRLVNIVPPEYQVVTMEMQIQIDQLEGKMVFAVPYMTIEPIRDKLKSGAQFDLMA
IDPQWSFRLSKELLEAPLDVSVEVGGAVISLNDLLSLVPGDTIMLDTPCTSDLTVKVGGVPKFTGMPGIRHGNKALQITN
VVGKGEQR

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=325, Percent_Identity=27.3846153846154, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36284; Mature: 36284

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKILTKQEIEALLAAVFEGKIEPDRELAKEEGTVHSYDLFNSEAHKGLVPNLDIIYDGF
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHEEHHH
IRYQRGTLSNRLGRIVEIKKLGAGSYKFDDFIQTLPSPVCMAIYKADPLKGAALIAFDST
HHCCCCCHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCH
LVFTIVDCILGGTGMTSVQTSANRMFTSIELRLVQKIVQDMLVDLEKAWAPLYAAKMSLL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMEMNPRLVNIVPPEYQVVTMEMQIQIDQLEGKMVFAVPYMTIEPIRDKLKSGAQFDLMA
HEECCCEEEEECCCCEEEEEEEEEEEEEECCCEEEEEECCEEHHHHHHHHHCCCCEEEEE
IDPQWSFRLSKELLEAPLDVSVEVGGAVISLNDLLSLVPGDTIMLDTPCTSDLTVKVGGV
ECCCCCHHHHHHHHCCCCCEEEECCCEEEEHHHHHHHCCCCEEEECCCCCCCCEEEECCC
PKFTGMPGIRHGNKALQITNVVGKGEQR
CCCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MEKILTKQEIEALLAAVFEGKIEPDRELAKEEGTVHSYDLFNSEAHKGLVPNLDIIYDGF
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHEEHHH
IRYQRGTLSNRLGRIVEIKKLGAGSYKFDDFIQTLPSPVCMAIYKADPLKGAALIAFDST
HHCCCCCHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCH
LVFTIVDCILGGTGMTSVQTSANRMFTSIELRLVQKIVQDMLVDLEKAWAPLYAAKMSLL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMEMNPRLVNIVPPEYQVVTMEMQIQIDQLEGKMVFAVPYMTIEPIRDKLKSGAQFDLMA
HEECCCEEEEECCCCEEEEEEEEEEEEEECCCEEEEEECCEEHHHHHHHHHCCCCEEEEE
IDPQWSFRLSKELLEAPLDVSVEVGGAVISLNDLLSLVPGDTIMLDTPCTSDLTVKVGGV
ECCCCCHHHHHHHHCCCCCEEEECCCEEEEHHHHHHHCCCCEEEECCCCCCCCEEEECCC
PKFTGMPGIRHGNKALQITNVVGKGEQR
CCCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]