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The map label for this gene is fliF [H]

Identifier: 39995518

GI number: 39995518

Start: 440788

End: 442371

Strand: Direct

Name: fliF [H]

Synonym: GSU0410

Alternate gene names: 39995518

Gene position: 440788-442371 (Clockwise)

Preceding gene: 39995517

Following gene: 39995519

Centisome position: 11.56

GC content: 61.11

Gene sequence:

>1584_bases
ATGCCTGAAGCCCTGAACAAACTGATCCAGCCTTTCATGGCACTGCCACCGGCCAAGCGTTGGGTGGTGGGAGGCGTTGT
CGGCCTGTCGGTCATCGCCTTCACCATCCTGATTCTGGTGGCGAACCGGACGGACTATCGCCCCCTCTTCACGAACCTGA
CGAGCGAGGATGCCGGCGAGATCGTGACCAAGCTCAAGGAGCAGAAGGTCCCCTATCGTATCGCCGCCGACGGCAAGGCG
ATCCTTGTCCCCTCCGACAAGGTGTACGATCTGCGGCTTTCGTTGGCCTCCGACGGCCTGCCCCAGGGGGGAGGAGTCGG
GTTCGAGATATTCGACCGGAAGAATTTCGGCATGACCGATTTCGTTCAGAAACTCAATTACCAGCGGGCTCTCCAAGGTG
AATTGTCTCGCACCATCAGCCAGATTTCAGGGGTGGAGCAGGCCCGGGTGCACCTGGTGATTCCCGAGAAGTCGCTTTTT
AAGGAGGACGAAAAGCCGGCCACGGCTTCGGTGGTCCTGAAGGTGAAGGGGCAGCGCCAGCTTCGTGAAAACGACGTGCA
GGGGATCGTCCACCTGGTTGCGTCCGCCATCGAGGGGATGAACCCGGAGCATGTGACGGTTCTCGATCAGAAGGGCAAGC
TCCTGTCCAAAAACACCCCCGGGGATGCAGCAGGCAAGATGACCGCCTCCATGCAGGAGGTCCAGCGGGCCTATGAACGG
AGTACCGAGGAACGCCTCCAGTCGCTGCTGGACAAAGCAGTGGGTGCCGGCAAGTCGGTGGCCCGGGTTTCGGCGGTCTT
CGATTTCCGCCAAGTGGAGCGCTACGAGGAAAAATACGACCCCGAGACCGTGGTCCGCAGTGAGCAGCGGTCCGAGGAGA
AGCAAGATGGCTCGACGGTCACGGGCGGGGTGCCCGGCGTGCAGACCAACCTGGGGCGCACGGCCGGCCAGCCCGCCGGA
ACCTCCGGCGGCGGCAGCAAGAACGACGAAACCCTCAACTATGAGGTGAGCCGCGCCACCGCCCGCACCATCGAACCGGT
GGGGACCCTCTCCAAGGTTTCTGTGGCGATTCTGGTGGACGGCAAGTATGATGCTGCCGCAGCCGGCAAGGACGGGAAAG
AGGCAAAACCCAAGTACACGCCCCGTTCGCCGGACGAACTCCAGAAGATCGATGCGCTGGTGAAAAGCTCGGTCGGCTTC
AATGTCGAGCGGGGCGATCAGGTGACGGTCGTCAATATACCGTTCCAGGATACGGGTGATGTCGGGGCTGGTGAGGCCGA
CAAATGGTGGAACGCCCCCATTTTCCTTTCCCTGCTGAAGAACGGCCTGATCGGCTTCGGCTTCCTGGCGCTCCTCCTTT
TCGTGGTGCGGCCGCTCCTGAAGACCCTCAAGCCGGAAAAGAGCACGAGCTTCGAGCCGATCCCCTCGGCCGAGGATGCC
CTCAACCAGATTGCAGAGATCCATCGGTTGCAGATCGGCAACCAGACCGTGAGCCAGATGGAGCTCATCAACAAGATCAA
GCAGGAACCCTATCAAGCGGCCCAGATCATTCAGAACTGGCTGCGAGACAAGGGTGAGGAATAG

Upstream 100 bases:

>100_bases
CTCGTTCCAGTTCCTGAGCCAGGTGCGGAACAAGGCGGTCGAGGCCTACCAGGAAGTCATGCGGATGCAGGTCTAGCCCT
GACACCCGCCGGAGTACTTC

Downstream 100 bases:

>100_bases
GGCTCGTATCCATGACAGGAACCGACAAGGCGGCCATACTGCTCCTCTACCTGGGTCCCGAAGCGACCTCAAAGGTATTC
GAGCACCTGGACGACGACGA

Product: flagellar M-ring protein FliF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MPEALNKLIQPFMALPPAKRWVVGGVVGLSVIAFTILILVANRTDYRPLFTNLTSEDAGEIVTKLKEQKVPYRIAADGKA
ILVPSDKVYDLRLSLASDGLPQGGGVGFEIFDRKNFGMTDFVQKLNYQRALQGELSRTISQISGVEQARVHLVIPEKSLF
KEDEKPATASVVLKVKGQRQLRENDVQGIVHLVASAIEGMNPEHVTVLDQKGKLLSKNTPGDAAGKMTASMQEVQRAYER
STEERLQSLLDKAVGAGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEEKQDGSTVTGGVPGVQTNLGRTAGQPAG
TSGGGSKNDETLNYEVSRATARTIEPVGTLSKVSVAILVDGKYDAAAAGKDGKEAKPKYTPRSPDELQKIDALVKSSVGF
NVERGDQVTVVNIPFQDTGDVGAGEADKWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLLKTLKPEKSTSFEPIPSAEDA
LNQIAEIHRLQIGNQTVSQMELINKIKQEPYQAAQIIQNWLRDKGEE

Sequences:

>Translated_527_residues
MPEALNKLIQPFMALPPAKRWVVGGVVGLSVIAFTILILVANRTDYRPLFTNLTSEDAGEIVTKLKEQKVPYRIAADGKA
ILVPSDKVYDLRLSLASDGLPQGGGVGFEIFDRKNFGMTDFVQKLNYQRALQGELSRTISQISGVEQARVHLVIPEKSLF
KEDEKPATASVVLKVKGQRQLRENDVQGIVHLVASAIEGMNPEHVTVLDQKGKLLSKNTPGDAAGKMTASMQEVQRAYER
STEERLQSLLDKAVGAGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEEKQDGSTVTGGVPGVQTNLGRTAGQPAG
TSGGGSKNDETLNYEVSRATARTIEPVGTLSKVSVAILVDGKYDAAAAGKDGKEAKPKYTPRSPDELQKIDALVKSSVGF
NVERGDQVTVVNIPFQDTGDVGAGEADKWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLLKTLKPEKSTSFEPIPSAEDA
LNQIAEIHRLQIGNQTVSQMELINKIKQEPYQAAQIIQNWLRDKGEE
>Mature_526_residues
PEALNKLIQPFMALPPAKRWVVGGVVGLSVIAFTILILVANRTDYRPLFTNLTSEDAGEIVTKLKEQKVPYRIAADGKAI
LVPSDKVYDLRLSLASDGLPQGGGVGFEIFDRKNFGMTDFVQKLNYQRALQGELSRTISQISGVEQARVHLVIPEKSLFK
EDEKPATASVVLKVKGQRQLRENDVQGIVHLVASAIEGMNPEHVTVLDQKGKLLSKNTPGDAAGKMTASMQEVQRAYERS
TEERLQSLLDKAVGAGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEEKQDGSTVTGGVPGVQTNLGRTAGQPAGT
SGGGSKNDETLNYEVSRATARTIEPVGTLSKVSVAILVDGKYDAAAAGKDGKEAKPKYTPRSPDELQKIDALVKSSVGFN
VERGDQVTVVNIPFQDTGDVGAGEADKWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLLKTLKPEKSTSFEPIPSAEDAL
NQIAEIHRLQIGNQTVSQMELINKIKQEPYQAAQIIQNWLRDKGEE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=447, Percent_Identity=38.7024608501119, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 57523; Mature: 57392

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEALNKLIQPFMALPPAKRWVVGGVVGLSVIAFTILILVANRTDYRPLFTNLTSEDAGE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHH
IVTKLKEQKVPYRIAADGKAILVPSDKVYDLRLSLASDGLPQGGGVGFEIFDRKNFGMTD
HHHHHHHHCCCEEEECCCCEEEECCCCEEEEEEEHHCCCCCCCCCCCEEEECCCCCCHHH
FVQKLNYQRALQGELSRTISQISGVEQARVHLVIPEKSLFKEDEKPATASVVLKVKGQRQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCCCCCEEEEEEECCHHH
LRENDVQGIVHLVASAIEGMNPEHVTVLDQKGKLLSKNTPGDAAGKMTASMQEVQRAYER
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHH
STEERLQSLLDKAVGAGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEEKQDGSTV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
TGGVPGVQTNLGRTAGQPAGTSGGGSKNDETLNYEVSRATARTIEPVGTLSKVSVAILVD
ECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHEEEEEEEC
GKYDAAAAGKDGKEAKPKYTPRSPDELQKIDALVKSSVGFNVERGDQVTVVNIPFQDTGD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC
VGAGEADKWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLLKTLKPEKSTSFEPIPSAEDA
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LNQIAEIHRLQIGNQTVSQMELINKIKQEPYQAAQIIQNWLRDKGEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PEALNKLIQPFMALPPAKRWVVGGVVGLSVIAFTILILVANRTDYRPLFTNLTSEDAGE
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHH
IVTKLKEQKVPYRIAADGKAILVPSDKVYDLRLSLASDGLPQGGGVGFEIFDRKNFGMTD
HHHHHHHHCCCEEEECCCCEEEECCCCEEEEEEEHHCCCCCCCCCCCEEEECCCCCCHHH
FVQKLNYQRALQGELSRTISQISGVEQARVHLVIPEKSLFKEDEKPATASVVLKVKGQRQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCCCCCEEEEEEECCHHH
LRENDVQGIVHLVASAIEGMNPEHVTVLDQKGKLLSKNTPGDAAGKMTASMQEVQRAYER
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHH
STEERLQSLLDKAVGAGKSVARVSAVFDFRQVERYEEKYDPETVVRSEQRSEEKQDGSTV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
TGGVPGVQTNLGRTAGQPAGTSGGGSKNDETLNYEVSRATARTIEPVGTLSKVSVAILVD
ECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHEEEEEEEC
GKYDAAAAGKDGKEAKPKYTPRSPDELQKIDALVKSSVGFNVERGDQVTVVNIPFQDTGD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC
VGAGEADKWWNAPIFLSLLKNGLIGFGFLALLLFVVRPLLKTLKPEKSTSFEPIPSAEDA
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LNQIAEIHRLQIGNQTVSQMELINKIKQEPYQAAQIIQNWLRDKGEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1551848; 8224881 [H]