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The map label for this gene is intZ [H]

Identifier: 39995175

GI number: 39995175

Start: 79725

End: 81044

Strand: Reverse

Name: intZ [H]

Synonym: GSU0064

Alternate gene names: 39995175

Gene position: 81044-79725 (Counterclockwise)

Preceding gene: 39995182

Following gene: 39995170

Centisome position: 2.12

GC content: 55.0

Gene sequence:

>1320_bases
ATGGCACGGCAGTCTACCAAGTTTAGCGATAAGTTTCTCCGGGCTCTAAAGCCGCAGGAAAAGCCCTATGACATTCGCGA
AGGTGATGGCTTCGGAGTGCGAGTCTTCCCCAGCGGGGCAAGATCATTTTTCTACATGTACCACTTTGACGGGAAGCGGC
GCTTCTTGAACCTCGGCAAATACCCCCAGCTCTCATTGGCCGATGCGAGGCAGAAGCACCGAGATGCAGTTTCACAGCAC
GCTAAGGGCATCGACCCCTTGGCTGCCACACAAGAACAGAAGCGGGAGCGACGCGAGGCGATAACCGTGGCCGACCTCAT
CGACGAATACGTTAAGAACTGGGCCAAGGTGTACAAGGTCGAGCGATCCGCCACCGAGGATGAGCGAGCCCTGTCGAAGG
ATGTTCTCCCTTACTGGGGCAAGAGGAAAGCTAAAGACATCAAACGGACCGACGCCATCGCCCTGCTTGAGCGCATAGTC
CGGCGAGGCTCTCCTGGCCAAGCGCGGAACATTCTGAAGGTGGGCCGCAAAATGTATGAGTTCGCCATCGAGAGGAAGTA
CGTGGAAGCTAACCCGTTTTCAAGAATAAGCAAGGCGGTGCCGGCAACTATGCCGCGCACAAGGTCAAGAGTGCTAACCG
AAAGCGAAATTAAATACATCTGGAGCGCCATCGATAAAAGTGCGGGCTCGGACGAGACGAAGCGCGCTTTGAAATTGATC
TTGGTGACCGCGCAGCGGCCCGGAGAAGTCATCGGTATGCATCGCAGAGAGATCGATGGCGAGTGGTGGACAATCCCACC
TGAGAGAATAAAAACAGAAAAGGACCGCACTCGGTCCAATTCGGTAGGCCCACAACCTCATAGGGTCTACCTCTCTCCAC
TGGCGCTTGAGCTCATCGGCGATAAAGATGGCTACATTTTCGAGAGCCCCCGTGGTGGCGCGATGGACTCCAAAGCTCTA
TCCCACGTGGTTCATGCAGCTCCACTGGTGCCTGAGCTCGTCAGAGACACAGAGGGCCATGTTTTCGCAGGCCCCTGTGG
CCCTGTAGCTGCCAAAGCTCTGATGCAGCTACTTGAGAAAGAAATCGCGTACCCCGACAAGGAGAAATACTACGGTCTGA
CTCGATGGACCCCTCACGACCTACGGAGGACAGCACGTACTAGAATGTCCGCCATCAACATTCCCACCAGGCACGCGGAA
AGAGTGCTGAACCACACCCTCCCAGGCATCCAGAAAACATATGATTTGTATCGTTACGATCCTCAAAAAAAAGCCGCCCT
GACCAAATGGGCCGCATATCTCGAAGAGCTACTACGGTAG

Upstream 100 bases:

>100_bases
ATCCTATTGGAGGCCACGGAAGAGGCCACGCGAAAAATTCCGAATGCCTCCCCTTAACACCCTCCGGATATTCCGAATAT
CCACAAAAAGGAGGGTACCA

Downstream 100 bases:

>100_bases
TAATTTACGATCTGAGCAAGGGCGGCTCATAGAGCCGCCCGCACCTCGCCCACCTCGCCTACCGTTGCAGTTGAACAGAG
CGGCCGGGTCCTCCGCCACT

Product: integrative genetic element Gsu32, integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MARQSTKFSDKFLRALKPQEKPYDIREGDGFGVRVFPSGARSFFYMYHFDGKRRFLNLGKYPQLSLADARQKHRDAVSQH
AKGIDPLAATQEQKRERREAITVADLIDEYVKNWAKVYKVERSATEDERALSKDVLPYWGKRKAKDIKRTDAIALLERIV
RRGSPGQARNILKVGRKMYEFAIERKYVEANPFSRISKAVPATMPRTRSRVLTESEIKYIWSAIDKSAGSDETKRALKLI
LVTAQRPGEVIGMHRREIDGEWWTIPPERIKTEKDRTRSNSVGPQPHRVYLSPLALELIGDKDGYIFESPRGGAMDSKAL
SHVVHAAPLVPELVRDTEGHVFAGPCGPVAAKALMQLLEKEIAYPDKEKYYGLTRWTPHDLRRTARTRMSAINIPTRHAE
RVLNHTLPGIQKTYDLYRYDPQKKAALTKWAAYLEELLR

Sequences:

>Translated_439_residues
MARQSTKFSDKFLRALKPQEKPYDIREGDGFGVRVFPSGARSFFYMYHFDGKRRFLNLGKYPQLSLADARQKHRDAVSQH
AKGIDPLAATQEQKRERREAITVADLIDEYVKNWAKVYKVERSATEDERALSKDVLPYWGKRKAKDIKRTDAIALLERIV
RRGSPGQARNILKVGRKMYEFAIERKYVEANPFSRISKAVPATMPRTRSRVLTESEIKYIWSAIDKSAGSDETKRALKLI
LVTAQRPGEVIGMHRREIDGEWWTIPPERIKTEKDRTRSNSVGPQPHRVYLSPLALELIGDKDGYIFESPRGGAMDSKAL
SHVVHAAPLVPELVRDTEGHVFAGPCGPVAAKALMQLLEKEIAYPDKEKYYGLTRWTPHDLRRTARTRMSAINIPTRHAE
RVLNHTLPGIQKTYDLYRYDPQKKAALTKWAAYLEELLR
>Mature_438_residues
ARQSTKFSDKFLRALKPQEKPYDIREGDGFGVRVFPSGARSFFYMYHFDGKRRFLNLGKYPQLSLADARQKHRDAVSQHA
KGIDPLAATQEQKRERREAITVADLIDEYVKNWAKVYKVERSATEDERALSKDVLPYWGKRKAKDIKRTDAIALLERIVR
RGSPGQARNILKVGRKMYEFAIERKYVEANPFSRISKAVPATMPRTRSRVLTESEIKYIWSAIDKSAGSDETKRALKLIL
VTAQRPGEVIGMHRREIDGEWWTIPPERIKTEKDRTRSNSVGPQPHRVYLSPLALELIGDKDGYIFESPRGGAMDSKALS
HVVHAAPLVPELVRDTEGHVFAGPCGPVAAKALMQLLEKEIAYPDKEKYYGLTRWTPHDLRRTARTRMSAINIPTRHAER
VLNHTLPGIQKTYDLYRYDPQKKAALTKWAAYLEELLR

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=448, Percent_Identity=27.2321428571429, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788974, Length=308, Percent_Identity=26.6233766233766, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788690, Length=430, Percent_Identity=22.7906976744186, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 50368; Mature: 50237

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQSTKFSDKFLRALKPQEKPYDIREGDGFGVRVFPSGARSFFYMYHFDGKRRFLNLGK
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHCCCC
YPQLSLADARQKHRDAVSQHAKGIDPLAATQEQKRERREAITVADLIDEYVKNWAKVYKV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSATEDERALSKDVLPYWGKRKAKDIKRTDAIALLERIVRRGSPGQARNILKVGRKMYE
HHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FAIERKYVEANPFSRISKAVPATMPRTRSRVLTESEIKYIWSAIDKSAGSDETKRALKLI
HHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LVTAQRPGEVIGMHRREIDGEWWTIPPERIKTEKDRTRSNSVGPQPHRVYLSPLALELIG
HEECCCCCHHHCCHHHHCCCCEEECCHHHHCCHHHHHHCCCCCCCCCEEEECHHHHHEEC
DKDGYIFESPRGGAMDSKALSHVVHAAPLVPELVRDTEGHVFAGPCGPVAAKALMQLLEK
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
EIAYPDKEKYYGLTRWTPHDLRRTARTRMSAINIPTRHAERVLNHTLPGIQKTYDLYRYD
HHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCC
PQKKAALTKWAAYLEELLR
CHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ARQSTKFSDKFLRALKPQEKPYDIREGDGFGVRVFPSGARSFFYMYHFDGKRRFLNLGK
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHCCCC
YPQLSLADARQKHRDAVSQHAKGIDPLAATQEQKRERREAITVADLIDEYVKNWAKVYKV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSATEDERALSKDVLPYWGKRKAKDIKRTDAIALLERIVRRGSPGQARNILKVGRKMYE
HHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
FAIERKYVEANPFSRISKAVPATMPRTRSRVLTESEIKYIWSAIDKSAGSDETKRALKLI
HHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LVTAQRPGEVIGMHRREIDGEWWTIPPERIKTEKDRTRSNSVGPQPHRVYLSPLALELIG
HEECCCCCHHHCCHHHHCCCCEEECCHHHHCCHHHHHHCCCCCCCCCEEEECHHHHHEEC
DKDGYIFESPRGGAMDSKALSHVVHAAPLVPELVRDTEGHVFAGPCGPVAAKALMQLLEK
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
EIAYPDKEKYYGLTRWTPHDLRRTARTRMSAINIPTRHAERVLNHTLPGIQKTYDLYRYD
HHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCC
PQKKAALTKWAAYLEELLR
CHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]