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The map label for this gene is trpB1 [H]

Identifier: 38234885

GI number: 38234885

Start: 2456701

End: 2458032

Strand: Direct

Name: trpB1 [H]

Synonym: DIP2351

Alternate gene names: 38234885

Gene position: 2456701-2458032 (Clockwise)

Preceding gene: 38234884

Following gene: 38234886

Centisome position: 98.72

GC content: 54.05

Gene sequence:

>1332_bases
ATGACTGACTCCACCCTCCGTAAGACGTTAATCAGCGCCTACTTTGGTAACTACGGCGGACAGTTTGTTCCCGATGCGCT
CCTGCCGGTTCTCGACGAACTAGAGCAGGCCTATGTGGATGCACTCAACGACCCAGAGTTTTCGCGGGAACTTGCCAGGC
TACAAAAGCACTACCTTGGTCGTCCCACTCCTATTACCGAATGCGCCAATCTTCCCCTAGCAGGTAAGGGGAAGGGTAAG
GTGCGGATCTTCCTCAAACGCGAGGATCTAGTTCACGGTGGCGCACATAAGGGCAATCAGGTGCTTGCCCAGGCGTTGAT
TGCTCGCCGATTGGGTAAAACTCGTTTGATCGCAGAAACCGGTGCTGGTCAGCACGGTACCGCCACTGCAATGGTGGCGG
CTTTGATGGGAATGGACTGCACCATTTATATGGGTGCTAAAGATGTGGCCCGCCAGCAGCCCAATGTGTATCGCATGCGG
CTCATGGGGGCTACGGTGGTGCCAGTAACCAACGAGCATGGTGATTCCATGTCGGATGCTATCGACGTAGCGCTTATGGA
CTGGGTGGAAAACTTGGATACCACGCACTATCTGATTGGTTCTGCGGTCGGCCCACATCCGTTCCCTACGCTGGTCAAGG
AGTTTCAGGCGGTTATTTCGCGGGAATCTCGCGAACAGATGCTGGAGCTAACAGGCAAGCTTCCGGACGCAGTAGTTGCT
GCCGTGGGCGGTGGTTCGAATGCTATTGGTGCGTTTGCTGATTATCTCACGGATAAGCCAGGTAATGAGTCCGTACGCTT
GATTGGTGTGGAGCCAGCAGGCGAAGGTCTTGATTCCGGTAAGCATGGTGCGCCGCTGCAGCGTGGCAAGGTTGGTGTGC
AACACGGCTTCAAGTCGTTCATCTTGGCTGATGAGCAGGGCAATCCGCTGCATTCTTATTCCGTATCGGCTGGTCTTGAT
TATCCAGGCGTGGGTCCAGAACACGCCCACCTCATGGAATCTGGTCGTGCGCAATATTATGGAATCACCGATGCCGAGGC
GCTTCAAGCATTCCGGATGCTGAGCCGCTACGAGGGTATCATTCCAGCTTTGGAGTCTAGCCACGCTCTAGCATATGCGT
TAAAGATGGCAGAAAACGCCGAAGAAACCGATGCTCCCATTAACATTGTGGTCAATCTTTCGGGTCGTGGCGATAAAGAT
GTGGACTATGTTCGTAATATCTTGGGCGATCTTGCTACCGAGGATCCAGCAACCTCACCAGTCACGGATCCTCATGTCGC
CGAAACCCTCGTCACAATGATCGCTGAGTCCAATGCACGAGAGGGAGTCTAA

Upstream 100 bases:

>100_bases
GCGGGCCTTTTTGTTAGCCACCAACAAGCTCACAAGAGTTTCGGCCCACCGCTTGAACACAACAAATATTTGTTTTTCAG
CGAAAGAGGTTAGAAACACC

Downstream 100 bases:

>100_bases
GCATGGCAGATTTCCATGCATCCGCCCGCGAGGTGCGCTATCACGAGGATGCCGCCGACCTCTTTCATCATCTTGGCGGG
ACCTCGGCTAGTGACTCCGT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTDSTLRKTLISAYFGNYGGQFVPDALLPVLDELEQAYVDALNDPEFSRELARLQKHYLGRPTPITECANLPLAGKGKGK
VRIFLKREDLVHGGAHKGNQVLAQALIARRLGKTRLIAETGAGQHGTATAMVAALMGMDCTIYMGAKDVARQQPNVYRMR
LMGATVVPVTNEHGDSMSDAIDVALMDWVENLDTTHYLIGSAVGPHPFPTLVKEFQAVISRESREQMLELTGKLPDAVVA
AVGGGSNAIGAFADYLTDKPGNESVRLIGVEPAGEGLDSGKHGAPLQRGKVGVQHGFKSFILADEQGNPLHSYSVSAGLD
YPGVGPEHAHLMESGRAQYYGITDAEALQAFRMLSRYEGIIPALESSHALAYALKMAENAEETDAPINIVVNLSGRGDKD
VDYVRNILGDLATEDPATSPVTDPHVAETLVTMIAESNAREGV

Sequences:

>Translated_443_residues
MTDSTLRKTLISAYFGNYGGQFVPDALLPVLDELEQAYVDALNDPEFSRELARLQKHYLGRPTPITECANLPLAGKGKGK
VRIFLKREDLVHGGAHKGNQVLAQALIARRLGKTRLIAETGAGQHGTATAMVAALMGMDCTIYMGAKDVARQQPNVYRMR
LMGATVVPVTNEHGDSMSDAIDVALMDWVENLDTTHYLIGSAVGPHPFPTLVKEFQAVISRESREQMLELTGKLPDAVVA
AVGGGSNAIGAFADYLTDKPGNESVRLIGVEPAGEGLDSGKHGAPLQRGKVGVQHGFKSFILADEQGNPLHSYSVSAGLD
YPGVGPEHAHLMESGRAQYYGITDAEALQAFRMLSRYEGIIPALESSHALAYALKMAENAEETDAPINIVVNLSGRGDKD
VDYVRNILGDLATEDPATSPVTDPHVAETLVTMIAESNAREGV
>Mature_442_residues
TDSTLRKTLISAYFGNYGGQFVPDALLPVLDELEQAYVDALNDPEFSRELARLQKHYLGRPTPITECANLPLAGKGKGKV
RIFLKREDLVHGGAHKGNQVLAQALIARRLGKTRLIAETGAGQHGTATAMVAALMGMDCTIYMGAKDVARQQPNVYRMRL
MGATVVPVTNEHGDSMSDAIDVALMDWVENLDTTHYLIGSAVGPHPFPTLVKEFQAVISRESREQMLELTGKLPDAVVAA
VGGGSNAIGAFADYLTDKPGNESVRLIGVEPAGEGLDSGKHGAPLQRGKVGVQHGFKSFILADEQGNPLHSYSVSAGLDY
PGVGPEHAHLMESGRAQYYGITDAEALQAFRMLSRYEGIIPALESSHALAYALKMAENAEETDAPINIVVNLSGRGDKDV
DYVRNILGDLATEDPATSPVTDPHVAETLVTMIAESNAREGV

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=400, Percent_Identity=56.25, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321412, Length=398, Percent_Identity=50.5025125628141, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 47455; Mature: 47324

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSTLRKTLISAYFGNYGGQFVPDALLPVLDELEQAYVDALNDPEFSRELARLQKHYLG
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
RPTPITECANLPLAGKGKGKVRIFLKREDLVHGGAHKGNQVLAQALIARRLGKTRLIAET
CCCCHHHHCCCCCCCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEC
GAGQHGTATAMVAALMGMDCTIYMGAKDVARQQPNVYRMRLMGATVVPVTNEHGDSMSDA
CCCCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCEEEEEECCEEEEEECCCCCCHHHH
IDVALMDWVENLDTTHYLIGSAVGPHPFPTLVKEFQAVISRESREQMLELTGKLPDAVVA
HHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEE
AVGGGSNAIGAFADYLTDKPGNESVRLIGVEPAGEGLDSGKHGAPLQRGKVGVQHGFKSF
EECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCCHHHCCHHE
ILADEQGNPLHSYSVSAGLDYPGVGPEHAHLMESGRAQYYGITDAEALQAFRMLSRYEGI
EEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCC
IPALESSHALAYALKMAENAEETDAPINIVVNLSGRGDKDVDYVRNILGDLATEDPATSP
CCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC
VTDPHVAETLVTMIAESNAREGV
CCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDSTLRKTLISAYFGNYGGQFVPDALLPVLDELEQAYVDALNDPEFSRELARLQKHYLG
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
RPTPITECANLPLAGKGKGKVRIFLKREDLVHGGAHKGNQVLAQALIARRLGKTRLIAET
CCCCHHHHCCCCCCCCCCCCEEEEEEECCHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEC
GAGQHGTATAMVAALMGMDCTIYMGAKDVARQQPNVYRMRLMGATVVPVTNEHGDSMSDA
CCCCCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCCEEEEEECCEEEEEECCCCCCHHHH
IDVALMDWVENLDTTHYLIGSAVGPHPFPTLVKEFQAVISRESREQMLELTGKLPDAVVA
HHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEE
AVGGGSNAIGAFADYLTDKPGNESVRLIGVEPAGEGLDSGKHGAPLQRGKVGVQHGFKSF
EECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHCCCCHHHCCHHE
ILADEQGNPLHSYSVSAGLDYPGVGPEHAHLMESGRAQYYGITDAEALQAFRMLSRYEGI
EEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCC
IPALESSHALAYALKMAENAEETDAPINIVVNLSGRGDKDVDYVRNILGDLATEDPATSP
CCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC
VTDPHVAETLVTMIAESNAREGV
CCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]