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The map label for this gene is rihA [H]

Identifier: 38234874

GI number: 38234874

Start: 2446717

End: 2447712

Strand: Direct

Name: rihA [H]

Synonym: DIP2340

Alternate gene names: 38234874

Gene position: 2446717-2447712 (Clockwise)

Preceding gene: 38234872

Following gene: 38234875

Centisome position: 98.32

GC content: 59.54

Gene sequence:

>996_bases
ATGTTTCACGTGAAACTCACACTGACCACTATGTTGGACACCATGAATAGCTCAGCACATCGCCCCGTAGTTGTTGCCGA
CGTTGATACCGGCATTGACGACGCCCTCGCCATCACATACCTCGGCTACCTGCACCGACGCGGACTCATCGAGCTACGCA
TTACCACCTCGGCCGGCAACTGCACCGCCGAGCAAGCCGCAGCCAACTCAGCAGAGATCATGAACTCGCTCACACTCAGC
GACGTACCGATCACACCCGGCGCGCCGAAACCACGCGCACTGCCGCTAACCACCACCCCAGAAACCCACGGCCCCACGGG
TTTGGGTTATCACACCACCCGCGCGACCATACCGCAGCTTAGCAACGCAGCGGCCGCCGTCGATCTGTGGAAAGGTGCTG
ATTATCTCCTCGTAGCGGGCCCTGCCACCAACGTCGCGTGGGCAGCAGAAAACGCCCCTGAGGTTCTCAACGCCATCCCG
CACGTGACCTTTATGACGGGCGCTTTCCATTACCCAGGCAACACCACACCCACCGCCGAGTGGAATGCGTGGGTTGATCC
GCATGCACTCAAAGAGGCGCTCGCGCATTACCGCGGCCACGCTGTCCGGCCCACGATATGTCCACTCAACGTTACGGAGT
CCGTGCTCCTTACCCCACAACGCCTTAAGCAATGGTGCCAGCGCAGCCAGGAACCAGTCCTTCCGATACTTAGCGACGCC
CTCCGCTTCTACTTCGAATTCCATGAATCGGTCGGGGTGGGGTACATGGCCCAAATCCATGATCTCGCCGCTGCGATGGT
GCTCCTCAACCGCGTCCCACACACCACCCGCCCAGGGTTTGTGGATGTGGAAGCAGACGCGGAACTGACCCGCGGCACCA
CCGTGGTGGATTGGGACAGTAACTATGGCGATCATCCTGCCAACGCTCAGATTCTCACAGCCCTCCAATCGGAGGATGTG
TTCGCAGAGTTCGAACGGGTTGTATTAGAGTACTAA

Upstream 100 bases:

>100_bases
CGATGACTGCAGTGGCTGTGGCACAGGCACGACGCACGCTGGAAGAAATAGAGGGGAATCGCATGCAAAGAATCCTAATG
GGTTAGGTAGCAAAACCGTA

Downstream 100 bases:

>100_bases
CTGCTTTTGCGCAGCACTGCTCCGAGGTGACATAGGAGGAGTGTCCTTTTTGCAGCGCTTTGCTTTGTACCCCTTCGACA
CTCCTAGGAATACCTATGCA

Product: putative nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MFHVKLTLTTMLDTMNSSAHRPVVVADVDTGIDDALAITYLGYLHRRGLIELRITTSAGNCTAEQAAANSAEIMNSLTLS
DVPITPGAPKPRALPLTTTPETHGPTGLGYHTTRATIPQLSNAAAAVDLWKGADYLLVAGPATNVAWAAENAPEVLNAIP
HVTFMTGAFHYPGNTTPTAEWNAWVDPHALKEALAHYRGHAVRPTICPLNVTESVLLTPQRLKQWCQRSQEPVLPILSDA
LRFYFEFHESVGVGYMAQIHDLAAAMVLLNRVPHTTRPGFVDVEADAELTRGTTVVDWDSNYGDHPANAQILTALQSEDV
FAEFERVVLEY

Sequences:

>Translated_331_residues
MFHVKLTLTTMLDTMNSSAHRPVVVADVDTGIDDALAITYLGYLHRRGLIELRITTSAGNCTAEQAAANSAEIMNSLTLS
DVPITPGAPKPRALPLTTTPETHGPTGLGYHTTRATIPQLSNAAAAVDLWKGADYLLVAGPATNVAWAAENAPEVLNAIP
HVTFMTGAFHYPGNTTPTAEWNAWVDPHALKEALAHYRGHAVRPTICPLNVTESVLLTPQRLKQWCQRSQEPVLPILSDA
LRFYFEFHESVGVGYMAQIHDLAAAMVLLNRVPHTTRPGFVDVEADAELTRGTTVVDWDSNYGDHPANAQILTALQSEDV
FAEFERVVLEY
>Mature_331_residues
MFHVKLTLTTMLDTMNSSAHRPVVVADVDTGIDDALAITYLGYLHRRGLIELRITTSAGNCTAEQAAANSAEIMNSLTLS
DVPITPGAPKPRALPLTTTPETHGPTGLGYHTTRATIPQLSNAAAAVDLWKGADYLLVAGPATNVAWAAENAPEVLNAIP
HVTFMTGAFHYPGNTTPTAEWNAWVDPHALKEALAHYRGHAVRPTICPLNVTESVLLTPQRLKQWCQRSQEPVLPILSDA
LRFYFEFHESVGVGYMAQIHDLAAAMVLLNRVPHTTRPGFVDVEADAELTRGTTVVDWDSNYGDHPANAQILTALQSEDV
FAEFERVVLEY

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=302, Percent_Identity=31.1258278145695, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786213, Length=268, Percent_Identity=31.7164179104478, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788486, Length=315, Percent_Identity=26.3492063492064, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32565474, Length=303, Percent_Identity=28.3828382838284, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=261, Percent_Identity=25.2873563218391, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 36023; Mature: 36023

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHVKLTLTTMLDTMNSSAHRPVVVADVDTGIDDALAITYLGYLHRRGLIELRITTSAGN
CEEEEEEHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCC
CTAEQAAANSAEIMNSLTLSDVPITPGAPKPRALPLTTTPETHGPTGLGYHTTRATIPQL
CCHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHH
SNAAAAVDLWKGADYLLVAGPATNVAWAAENAPEVLNAIPHVTFMTGAFHYPGNTTPTAE
CCCHHHEEHCCCCCEEEEECCCCCEEECCCCCHHHHHHCCCEEEEECEEECCCCCCCCCC
WNAWVDPHALKEALAHYRGHAVRPTICPLNVTESVLLTPQRLKQWCQRSQEPVLPILSDA
CCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCHHHEECHHHHHHHHHCCCCCCHHHHHHH
LRFYFEFHESVGVGYMAQIHDLAAAMVLLNRVPHTTRPGFVDVEADAELTRGTTVVDWDS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCEEEECCC
NYGDHPANAQILTALQSEDVFAEFERVVLEY
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFHVKLTLTTMLDTMNSSAHRPVVVADVDTGIDDALAITYLGYLHRRGLIELRITTSAGN
CEEEEEEHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCC
CTAEQAAANSAEIMNSLTLSDVPITPGAPKPRALPLTTTPETHGPTGLGYHTTRATIPQL
CCHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHH
SNAAAAVDLWKGADYLLVAGPATNVAWAAENAPEVLNAIPHVTFMTGAFHYPGNTTPTAE
CCCHHHEEHCCCCCEEEEECCCCCEEECCCCCHHHHHHCCCEEEEECEEECCCCCCCCCC
WNAWVDPHALKEALAHYRGHAVRPTICPLNVTESVLLTPQRLKQWCQRSQEPVLPILSDA
CCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCHHHEECHHHHHHHHHCCCCCCHHHHHHH
LRFYFEFHESVGVGYMAQIHDLAAAMVLLNRVPHTTRPGFVDVEADAELTRGTTVVDWDS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCEEEECCC
NYGDHPANAQILTALQSEDVFAEFERVVLEY
CCCCCCCCCCEEEEHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA