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The map label for this gene is dhaK [H]

Identifier: 38234868

GI number: 38234868

Start: 2440685

End: 2441680

Strand: Direct

Name: dhaK [H]

Synonym: DIP2334

Alternate gene names: 38234868

Gene position: 2440685-2441680 (Clockwise)

Preceding gene: 38234867

Following gene: 38234869

Centisome position: 98.07

GC content: 54.82

Gene sequence:

>996_bases
ATGAAGAAACTCATTAATGATCCACATGATGTGGTCGCGGAAACCATTGCGGGTTTCGTGGCCGCACATAAGGATGTGGT
GACCTCCGATGGCCAGTTTGTTGAGCGTGCTGAACCTAAGGCACAGGGCAAGGTCGGGATCATTTCGGGTGGTGGTTCTG
GGCATGAGCCGCTGCATGCTGGGTTTGTTGGCTATGGCATGTTGGATGCCGCGGTGCCAGGGCCTGTGTTTACGTCTCCG
CCACCGGATCCGATTGTGGCTGCTACTAAGGCTGCGGATCATGGGGCGGGTGTGCTTTATATTGTGAAGAATTACACCGG
CGATGTACTGAATTTTGATACGGCTGCGGAGCTTGCTGAGTTTGATGACATTGAGGTGTCGCAGGTGATTGTCGATGATG
ATGCTGCGGTGGAGGATTCTCTTTATACTGCGGGTCGTCGTGGTGTTGCTGGCACGGTGTTGGTGGAGAAGATTGCGGGT
GCTGCGGCGGAGCGGGGCGATAATCTTGCGGAGGTTACTGCGGTTGCTACTGAGGTGGTGAAGAATACGCGGTCGATGGG
GGTTGCGCTTACGTCGTGCACGGTTCCGCATGTGGGTAAGCCGTCTTTTGATCTTGGTGATTCTGAGGTTGAGATTGGTA
TTGGTATTCATGGTGAGCCGGGTAGGCGTCGTGAGCCGATGAGTTCTGCGGATGCCATTACTGATCAGTTGTTGGATCCT
GTTGTGGAGGATCTGGGGCTTTGTGCGGGGGAGCGGGTTATCGCTTTGGTTAATGGTATGGGCGGTACGCCGTCGTCGGA
GTTGTATATCGTGTATCGCCGAGTGGCGGAGCGTCTTGGCCAGTTGGGCGTGGTTGTTGAGCGTTCGTTGGTGGGGAATT
ATGTGACGTCGTTGGATATGCAGGGGGCGTCGGTGACGTTGATGCGGGTGTCTGATGAGTTGCTTGAGCTTTTCGACGCC
CCGGTTAATACTGTGGCTTTGAGGAAGGGAATGTAG

Upstream 100 bases:

>100_bases
CGCGTAGTTCCACGTTAGGTATAGTGGGCACCATCCAGAATGCTTGTGACGTATATTCGCAGGTTAAGCACCATTTTTAG
GTGAAGGAGTGGTTGACAAA

Downstream 100 bases:

>100_bases
ATCATGGTTTTGGATACTCAATGGGCTTTGGCGTGGATTCGCGGTTGCGCGCGGTTGGCTTCTGAGCACCGTGAGGAGCT
GATTGATTTGGATCGTGCGA

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKLINDPHDVVAETIAGFVAAHKDVVTSDGQFVERAEPKAQGKVGIISGGGSGHEPLHAGFVGYGMLDAAVPGPVFTSP
PPDPIVAATKAADHGAGVLYIVKNYTGDVLNFDTAAELAEFDDIEVSQVIVDDDAAVEDSLYTAGRRGVAGTVLVEKIAG
AAAERGDNLAEVTAVATEVVKNTRSMGVALTSCTVPHVGKPSFDLGDSEVEIGIGIHGEPGRRREPMSSADAITDQLLDP
VVEDLGLCAGERVIALVNGMGGTPSSELYIVYRRVAERLGQLGVVVERSLVGNYVTSLDMQGASVTLMRVSDELLELFDA
PVNTVALRKGM

Sequences:

>Translated_331_residues
MKKLINDPHDVVAETIAGFVAAHKDVVTSDGQFVERAEPKAQGKVGIISGGGSGHEPLHAGFVGYGMLDAAVPGPVFTSP
PPDPIVAATKAADHGAGVLYIVKNYTGDVLNFDTAAELAEFDDIEVSQVIVDDDAAVEDSLYTAGRRGVAGTVLVEKIAG
AAAERGDNLAEVTAVATEVVKNTRSMGVALTSCTVPHVGKPSFDLGDSEVEIGIGIHGEPGRRREPMSSADAITDQLLDP
VVEDLGLCAGERVIALVNGMGGTPSSELYIVYRRVAERLGQLGVVVERSLVGNYVTSLDMQGASVTLMRVSDELLELFDA
PVNTVALRKGM
>Mature_331_residues
MKKLINDPHDVVAETIAGFVAAHKDVVTSDGQFVERAEPKAQGKVGIISGGGSGHEPLHAGFVGYGMLDAAVPGPVFTSP
PPDPIVAATKAADHGAGVLYIVKNYTGDVLNFDTAAELAEFDDIEVSQVIVDDDAAVEDSLYTAGRRGVAGTVLVEKIAG
AAAERGDNLAEVTAVATEVVKNTRSMGVALTSCTVPHVGKPSFDLGDSEVEIGIGIHGEPGRRREPMSSADAITDQLLDP
VVEDLGLCAGERVIALVNGMGGTPSSELYIVYRRVAERLGQLGVVVERSLVGNYVTSLDMQGASVTLMRVSDELLELFDA
PVNTVALRKGM

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=335, Percent_Identity=42.089552238806, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI87081857, Length=355, Percent_Identity=50.4225352112676, Blast_Score=348, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17565018, Length=322, Percent_Identity=37.888198757764, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321055, Length=293, Percent_Identity=41.9795221843003, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323570, Length=283, Percent_Identity=37.8091872791519, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34518; Mature: 34518

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINDPHDVVAETIAGFVAAHKDVVTSDGQFVERAEPKAQGKVGIISGGGSGHEPLHA
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEECCCCCCCCHHH
GFVGYGMLDAAVPGPVFTSPPPDPIVAATKAADHGAGVLYIVKNYTGDVLNFDTAAELAE
CHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEECCCHHHHHHH
FDDIEVSQVIVDDDAAVEDSLYTAGRRGVAGTVLVEKIAGAAAERGDNLAEVTAVATEVV
CCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KNTRSMGVALTSCTVPHVGKPSFDLGDSEVEIGIGIHGEPGRRREPMSSADAITDQLLDP
HHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
VVEDLGLCAGERVIALVNGMGGTPSSELYIVYRRVAERLGQLGVVVERSLVGNYVTSLDM
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
QGASVTLMRVSDELLELFDAPVNTVALRKGM
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MKKLINDPHDVVAETIAGFVAAHKDVVTSDGQFVERAEPKAQGKVGIISGGGSGHEPLHA
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEECCCCCCCCHHH
GFVGYGMLDAAVPGPVFTSPPPDPIVAATKAADHGAGVLYIVKNYTGDVLNFDTAAELAE
CHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEECCCHHHHHHH
FDDIEVSQVIVDDDAAVEDSLYTAGRRGVAGTVLVEKIAGAAAERGDNLAEVTAVATEVV
CCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KNTRSMGVALTSCTVPHVGKPSFDLGDSEVEIGIGIHGEPGRRREPMSSADAITDQLLDP
HHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
VVEDLGLCAGERVIALVNGMGGTPSSELYIVYRRVAERLGQLGVVVERSLVGNYVTSLDM
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
QGASVTLMRVSDELLELFDAPVNTVALRKGM
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]