Click here to switch to the map view.

The map label for this gene is lepA

Identifier: 38234335

GI number: 38234335

Start: 1809198

End: 1811045

Strand: Reverse

Name: lepA

Synonym: DIP1764

Alternate gene names: 38234335

Gene position: 1811045-1809198 (Counterclockwise)

Preceding gene: 38234338

Following gene: 38234334

Centisome position: 72.77

GC content: 57.03

Gene sequence:

>1848_bases
ATGGCCGAAAAATTCGCGGAGAAGACGTTCACGGATCCACAGCAGATCCGAAACTTCTGCATCATTGCACATATCGACCA
CGGTAAGTCCACGTTGGCTGACCGTATTTTGCAGCTGTCTAATGTTGTAGACGCACGCGACATGCGCGACCAGTACCTCG
ACAACATGGACATCGAACGCGAACGCGGCATTACCATCAAGGCCCAAAACGTCCGACTGCCATGGATCCCACGTACTGGC
CCCTACGCTGGCGAACAGATCGTCATGCAGATGATCGACACCCCAGGTCACGTGGACTTCACCTACGAAGTCTCTCGCGC
CTTGGAAGCTTGCGAAGGCGCAATCCTGCTTGTCGACGCAGCCCAAGGCATCGAAGCTCAAACACTGGCCAACCTCTACC
TGGCCATGGAAAACGATCTTGAGATCATCCCTGTTTTGAACAAGATCGACCTGCCAGCAGCCGATCCCGAAAAATACTCT
CTGGAAATCGCCAACATCATCGGCTGTGAACCAGAAGACGTATTGCGCGTGTCCGGCAAAACCGGTGAAGGCGTGGAAGA
ACTCCTCGACAAAGTCGCCGAGCTCATCCCCGCACCAACCACCGACTACCCAGACGATGCGCCCGCCCGCGCCATGATTT
TCGACTCGGTCTACGACACCTACCGTGGCGTAGTCACCTACATCCGCATGATCGACGGCAAGCTCACCCCACGCCAAAAG
ATCAAAATGATGTCTACCGGTGCCGTCCACGAACTCCTCGAAATTGGCATCGTCTCCCCAACACCAAAGAAGTGCTCCGG
CCTAGGACCTGGCGAAGTAGGCTACCTGATCACCGGTGTGAAAGACGTGCGCCAATCCAAGGTTGGTGACACCGTCACCT
GGGCACACAACGGCGCCGAAGAAGCACTCCAAGGCTACGAAGAGCCCAAGCCCATGGTCTACTCGGGTCTGTTCCCAATC
TCCCAGGCGGACTTCCCAGACCTACGCGACGCACTAGAAAAACTCCAGCTTAACGACGCCTCCCTCACCTACGAGCCAGA
AACCTCCGTAGCACTCGGCTTCGGCTTCCGCTGCGGCTTCCTCGGCCTGCTCCACATGGAGATCACTCGCGACCGCCTTC
AGCGCGAATTCGACCTCGACCTCATTTCCACCGCACCATCGGTGAACTACCGCGTTGTCGCCGAAGACGGCAGCGAACAC
CGCGTGCACAACCCATCCGACTGGCCAGCAGGCAAACTGCGCGAAGTCTACGAACCCATCGTGAAGACCACCATCATCGT
GCCTTCCGACTTCGTAGGCACCACCATGGAACTATGCCAAACCAAACGCGGCCAGATGGACGGCATGGACTACCTCTCCG
AGGACCGCGTAGAACTGCGCTACACCATGCCACTCGGCGAAATCATCTTCGACTTCTTCGACCAGCTGAAATCCCGCACC
AAGGGCTACGCATCCCTCAACTACGAAGAAGCCGGCGAACAACTCGCCGACCTAGTCAAGGTAGACATCCTGCTCCAAGG
CGACCCAGTGGACGCCTTCTCCGCCATCGTCCACCGCGACAACGCCCAGTGGTACGGCAACAAGATGACCAAGAAACTCA
AAGAGCTCATCCCACGCCAACAATTCGAAGTGCCCGTCCAGGCAGCCATCGGCTCCAAAGTGATCGCACGTGAAAACATC
CGCGCCCTGCGCAAAGACGTTCTCGCCAAATGCTACGGCGGCGACATCTCCCGAAAGCGCAAACTGCTAGAAAAGCAGAA
AGAAGGCAAGAAGCGCATGAAGAACATTGGTTCCGTTTCCGTGCCACAAGAGGCCTTCGTTGCGGCATTGAGCACCGACG
AAGGGTAG

Upstream 100 bases:

>100_bases
ATAGGGTCGATGGTGTGGGGGTCGTGTGTGGTCGGGGATGAGCGAAAAGTCGGGTAATGTAAGAGGGACTGTTTTGAGAA
AGGTACGAGTAAAAACAACC

Downstream 100 bases:

>100_bases
TTGCTTTTCGACGCACCCCCGCCTCCACATGAGCGTTCTGCTCAAAGTGGAGGCGGGGTTTCTTTGTTATCAGTGTTTTC
GCTTGAGATGAAATACACAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MAEKFAEKTFTDPQQIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIERERGITIKAQNVRLPWIPRTG
PYAGEQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPEKYS
LEIANIIGCEPEDVLRVSGKTGEGVEELLDKVAELIPAPTTDYPDDAPARAMIFDSVYDTYRGVVTYIRMIDGKLTPRQK
IKMMSTGAVHELLEIGIVSPTPKKCSGLGPGEVGYLITGVKDVRQSKVGDTVTWAHNGAEEALQGYEEPKPMVYSGLFPI
SQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGFLGLLHMEITRDRLQREFDLDLISTAPSVNYRVVAEDGSEH
RVHNPSDWPAGKLREVYEPIVKTTIIVPSDFVGTTMELCQTKRGQMDGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSRT
KGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRDNAQWYGNKMTKKLKELIPRQQFEVPVQAAIGSKVIARENI
RALRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKNIGSVSVPQEAFVAALSTDEG

Sequences:

>Translated_615_residues
MAEKFAEKTFTDPQQIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIERERGITIKAQNVRLPWIPRTG
PYAGEQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPEKYS
LEIANIIGCEPEDVLRVSGKTGEGVEELLDKVAELIPAPTTDYPDDAPARAMIFDSVYDTYRGVVTYIRMIDGKLTPRQK
IKMMSTGAVHELLEIGIVSPTPKKCSGLGPGEVGYLITGVKDVRQSKVGDTVTWAHNGAEEALQGYEEPKPMVYSGLFPI
SQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGFLGLLHMEITRDRLQREFDLDLISTAPSVNYRVVAEDGSEH
RVHNPSDWPAGKLREVYEPIVKTTIIVPSDFVGTTMELCQTKRGQMDGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSRT
KGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRDNAQWYGNKMTKKLKELIPRQQFEVPVQAAIGSKVIARENI
RALRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKNIGSVSVPQEAFVAALSTDEG
>Mature_614_residues
AEKFAEKTFTDPQQIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIERERGITIKAQNVRLPWIPRTGP
YAGEQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPEKYSL
EIANIIGCEPEDVLRVSGKTGEGVEELLDKVAELIPAPTTDYPDDAPARAMIFDSVYDTYRGVVTYIRMIDGKLTPRQKI
KMMSTGAVHELLEIGIVSPTPKKCSGLGPGEVGYLITGVKDVRQSKVGDTVTWAHNGAEEALQGYEEPKPMVYSGLFPIS
QADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGFLGLLHMEITRDRLQREFDLDLISTAPSVNYRVVAEDGSEHR
VHNPSDWPAGKLREVYEPIVKTTIIVPSDFVGTTMELCQTKRGQMDGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSRTK
GYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRDNAQWYGNKMTKKLKELIPRQQFEVPVQAAIGSKVIARENIR
ALRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKNIGSVSVPQEAFVAALSTDEG

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=46.0526315789474, Blast_Score=582, Evalue=1e-166,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=41.1764705882353, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI18390331, Length=151, Percent_Identity=34.4370860927152, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI25306283, Length=151, Percent_Identity=41.0596026490066, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI19923640, Length=151, Percent_Identity=41.0596026490066, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI25306287, Length=151, Percent_Identity=41.0596026490066, Blast_Score=93, Evalue=1e-18,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=35.6164383561644, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310132016, Length=114, Percent_Identity=37.719298245614, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI310110807, Length=114, Percent_Identity=37.719298245614, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI310123363, Length=114, Percent_Identity=37.719298245614, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI217272894, Length=149, Percent_Identity=31.5436241610738, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI217272892, Length=149, Percent_Identity=31.5436241610738, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI53729339, Length=211, Percent_Identity=27.9620853080569, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI53729337, Length=211, Percent_Identity=27.9620853080569, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=54.3478260869565, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI48994988, Length=517, Percent_Identity=27.0793036750484, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790835, Length=181, Percent_Identity=30.3867403314917, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789738, Length=192, Percent_Identity=32.2916666666667, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789559, Length=287, Percent_Identity=25.4355400696864, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=619, Percent_Identity=40.5492730210016, Blast_Score=463, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71988811, Length=138, Percent_Identity=37.6811594202899, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=138, Percent_Identity=37.6811594202899, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=152, Percent_Identity=40.1315789473684, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556745, Length=146, Percent_Identity=36.3013698630137, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=29.9363057324841, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17552882, Length=153, Percent_Identity=30.718954248366, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=605, Percent_Identity=43.4710743801653, Blast_Score=511, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6323098, Length=189, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324707, Length=206, Percent_Identity=30.0970873786408, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320593, Length=206, Percent_Identity=30.0970873786408, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322359, Length=118, Percent_Identity=39.8305084745763, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=147, Percent_Identity=39.4557823129252, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=247, Percent_Identity=27.5303643724696, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI78706572, Length=606, Percent_Identity=42.9042904290429, Blast_Score=516, Evalue=1e-146,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=42.7536231884058, Blast_Score=107, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=36.241610738255, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=34.2281879194631, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=34.2281879194631, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=34.2281879194631, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221458488, Length=160, Percent_Identity=34.375, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357743, Length=151, Percent_Identity=31.1258278145695, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_CORDI (P60931)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940102.1
- ProteinModelPortal:   P60931
- SMR:   P60931
- GeneID:   2649389
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1764
- NMPDR:   fig|257309.1.peg.1693
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   CDIP257309:DIP1764-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 68641; Mature: 68510

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKFAEKTFTDPQQIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIER
CCCHHHHHCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ERGITIKAQNVRLPWIPRTGPYAGEQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDA
HCCCEEEECCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCEEEEEC
AQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPEKYSLEIANIIGCEPEDVLRVSGK
CCCCCHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCHHEEEEHHHHCCCHHHHEEECCC
TGEGVEELLDKVAELIPAPTTDYPDDAPARAMIFDSVYDTYRGVVTYIRMIDGKLTPRQK
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IKMMSTGAVHELLEIGIVSPTPKKCSGLGPGEVGYLITGVKDVRQSKVGDTVTWAHNGAE
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHCHHHHHHHHCCCCEEECCCCHH
EALQGYEEPKPMVYSGLFPISQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGF
HHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHH
LGLLHMEITRDRLQREFDLDLISTAPSVNYRVVAEDGSEHRVHNPSDWPAGKLREVYEPI
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
VKTTIIVPSDFVGTTMELCQTKRGQMDGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSRT
HHEEEECCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHC
KGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRDNAQWYGNKMTKKLKELIPRQ
CCCEECCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHH
QFEVPVQAAIGSKVIARENIRALRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKNIGSVS
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
VPQEAFVAALSTDEG
CCHHHHHHHHCCCCC
>Mature Secondary Structure 
AEKFAEKTFTDPQQIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIER
CCHHHHHCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ERGITIKAQNVRLPWIPRTGPYAGEQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDA
HCCCEEEECCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCEEEEEC
AQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPEKYSLEIANIIGCEPEDVLRVSGK
CCCCCHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCHHEEEEHHHHCCCHHHHEEECCC
TGEGVEELLDKVAELIPAPTTDYPDDAPARAMIFDSVYDTYRGVVTYIRMIDGKLTPRQK
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IKMMSTGAVHELLEIGIVSPTPKKCSGLGPGEVGYLITGVKDVRQSKVGDTVTWAHNGAE
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHCHHHHHHHHCCCCEEECCCCHH
EALQGYEEPKPMVYSGLFPISQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGF
HHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHH
LGLLHMEITRDRLQREFDLDLISTAPSVNYRVVAEDGSEHRVHNPSDWPAGKLREVYEPI
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
VKTTIIVPSDFVGTTMELCQTKRGQMDGMDYLSEDRVELRYTMPLGEIIFDFFDQLKSRT
HHEEEECCCCHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHC
KGYASLNYEEAGEQLADLVKVDILLQGDPVDAFSAIVHRDNAQWYGNKMTKKLKELIPRQ
CCCEECCHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHH
QFEVPVQAAIGSKVIARENIRALRKDVLAKCYGGDISRKRKLLEKQKEGKKRMKNIGSVS
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
VPQEAFVAALSTDEG
CCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14602910