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The map label for this gene is tyrS

Identifier: 38233766

GI number: 38233766

Start: 1168427

End: 1169689

Strand: Direct

Name: tyrS

Synonym: DIP1176

Alternate gene names: 38233766

Gene position: 1168427-1169689 (Clockwise)

Preceding gene: 38233765

Following gene: 38233767

Centisome position: 46.95

GC content: 46.95

Gene sequence:

>1263_bases
ATGAACATTATCGACGAACTCCAATGGCGTGGCCTGATTAATCAGTCCACCGACATCGACGCGTTGCGAGAAGCATGCGA
AAAACCGATCACACTTTACTGCGGGTTTGATCCAACAGGTGACTCTCTACACGCCGGTCACTTGGTACCGATGATTATGC
TCCGACGTTTCCAAGAAGCTGGTCACCGACCGATTACTCTTGCTGGGGGTGCAACAGGTTTTATTGGGGATCCACGCGAC
GTGGGGGAGCGTTCTATGCTGTCCCAAGATACTATCGAGCACAATCTTGAGTCGATTAAAAAACAACTTCGGTCATTTGT
CCGATTTGATGGTGATAGTCCAGCCATCATGGTGAACAATGCTGACTGGACTATGTCTATGTCAGTAGTTGATTTTTTGC
GCGATGTCGGAAAAAACTTCTCTCTAAATACGATGCTGGATCGTGAAACTGTCAAACGACGCTTGGAAACAGACGGAATC
TCTTATACAGAGTTTTCCTACATGCTGCTCCAAGCTAATGATTTTGTTCAGCTGAATCGTGAATACGACTGTGTCCTGCA
AATCGGTGGTGGAGATCAATGGGGCAACATTGTTTCGGGTGTTGATCTCAATCGTCGAGTATCCGGAACAAAAACTCATG
CACTGACCGTTCCTCTTGTAACTGATGCCCAGGGGCAGAAATTTGGTAAGTCGACGGGCGGCGGAAAGCTTTGGCTTGAT
CCGGAGAAAACCTCTGCATATTCGTGGTATCAGTATTTCCTCAATGCGGGAGATTCTGTCGTCATTGATTACCTTCGATG
GTTTACCTTCTTAACTCAAGAAGAAATCGCTGAATTAGAAACTGAAGTACGGGAGCGTCCTCACCTAAGAGCTGCGCAAC
GCCGACTTGCTCAAGAGATGACTGATTTAGTCCATGGTCACGATGCGACTGTCTCGGTTGAGTTGGCTGCTCAAGCACTT
TTCGGCCGTGCTTCTTTGCAAGATCTAGATGAAGCTACGTTGGATGGTGCATTGTCCGAAACCGTAATTTTTGAAGTTCC
CGCGGGTGAATCGCCTACGATTATCGATCTTCTCATCGGTGCAGGTCTTGTTGATTCGAAGGGAGCTGCGCGTAGAACAA
TTAAAGAAGGCGGCGCATACGTCAATAATGAGCGAATTGAATCAGAAGATTGGCAACCAACTGATGACGTATTGCTACAT
GGAGCATGGTTAGTTCTTCGTAAAGGAAAGAAAAACTTTGCAGGTGTGAAGTACTCCGCGTAA

Upstream 100 bases:

>100_bases
CGCCTCGAGATTGCCTATCGAATTGATGACGGCATTCCAGTAATGCTTGTCGACGAATCCATCGCGTGGCCACCAGCAGA
AAAATAATTAGGGATCTTTT

Downstream 100 bases:

>100_bases
AGATCGATGTTTAAGGTCCAATCTCATGTTTTGTCGTGAGATTGGACCTTTAAGACTTTTAATATCTTCAAAAACCTGTC
TACCTGCGGGTTTGTGTGTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNIIDELQWRGLINQSTDIDALREACEKPITLYCGFDPTGDSLHAGHLVPMIMLRRFQEAGHRPITLAGGATGFIGDPRD
VGERSMLSQDTIEHNLESIKKQLRSFVRFDGDSPAIMVNNADWTMSMSVVDFLRDVGKNFSLNTMLDRETVKRRLETDGI
SYTEFSYMLLQANDFVQLNREYDCVLQIGGGDQWGNIVSGVDLNRRVSGTKTHALTVPLVTDAQGQKFGKSTGGGKLWLD
PEKTSAYSWYQYFLNAGDSVVIDYLRWFTFLTQEEIAELETEVRERPHLRAAQRRLAQEMTDLVHGHDATVSVELAAQAL
FGRASLQDLDEATLDGALSETVIFEVPAGESPTIIDLLIGAGLVDSKGAARRTIKEGGAYVNNERIESEDWQPTDDVLLH
GAWLVLRKGKKNFAGVKYSA

Sequences:

>Translated_420_residues
MNIIDELQWRGLINQSTDIDALREACEKPITLYCGFDPTGDSLHAGHLVPMIMLRRFQEAGHRPITLAGGATGFIGDPRD
VGERSMLSQDTIEHNLESIKKQLRSFVRFDGDSPAIMVNNADWTMSMSVVDFLRDVGKNFSLNTMLDRETVKRRLETDGI
SYTEFSYMLLQANDFVQLNREYDCVLQIGGGDQWGNIVSGVDLNRRVSGTKTHALTVPLVTDAQGQKFGKSTGGGKLWLD
PEKTSAYSWYQYFLNAGDSVVIDYLRWFTFLTQEEIAELETEVRERPHLRAAQRRLAQEMTDLVHGHDATVSVELAAQAL
FGRASLQDLDEATLDGALSETVIFEVPAGESPTIIDLLIGAGLVDSKGAARRTIKEGGAYVNNERIESEDWQPTDDVLLH
GAWLVLRKGKKNFAGVKYSA
>Mature_420_residues
MNIIDELQWRGLINQSTDIDALREACEKPITLYCGFDPTGDSLHAGHLVPMIMLRRFQEAGHRPITLAGGATGFIGDPRD
VGERSMLSQDTIEHNLESIKKQLRSFVRFDGDSPAIMVNNADWTMSMSVVDFLRDVGKNFSLNTMLDRETVKRRLETDGI
SYTEFSYMLLQANDFVQLNREYDCVLQIGGGDQWGNIVSGVDLNRRVSGTKTHALTVPLVTDAQGQKFGKSTGGGKLWLD
PEKTSAYSWYQYFLNAGDSVVIDYLRWFTFLTQEEIAELETEVRERPHLRAAQRRLAQEMTDLVHGHDATVSVELAAQAL
FGRASLQDLDEATLDGALSETVIFEVPAGESPTIIDLLIGAGLVDSKGAARRTIKEGGAYVNNERIESEDWQPTDDVLLH
GAWLVLRKGKKNFAGVKYSA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=409, Percent_Identity=34.9633251833741, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1787925, Length=418, Percent_Identity=45.933014354067, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145802, Length=412, Percent_Identity=34.9514563106796, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25145805, Length=314, Percent_Identity=33.7579617834395, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6325160, Length=437, Percent_Identity=36.3844393592677, Blast_Score=249, Evalue=7e-67,
Organism=Drosophila melanogaster, GI20130383, Length=406, Percent_Identity=37.9310344827586, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CORDI (Q6NHG2)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939533.1
- ProteinModelPortal:   Q6NHG2
- SMR:   Q6NHG2
- GeneID:   2649728
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1176
- NMPDR:   fig|257309.1.peg.1124
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- PhylomeDB:   Q6NHG2
- ProtClustDB:   PRK05912
- BioCyc:   CDIP257309:DIP1176-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46751; Mature: 46751

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 33-33 BINDING 167-167 BINDING 171-171 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDELQWRGLINQSTDIDALREACEKPITLYCGFDPTGDSLHAGHLVPMIMLRRFQEA
CCCHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GHRPITLAGGATGFIGDPRDVGERSMLSQDTIEHNLESIKKQLRSFVRFDGDSPAIMVNN
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
ADWTMSMSVVDFLRDVGKNFSLNTMLDRETVKRRLETDGISYTEFSYMLLQANDFVQLNR
CCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCEEEECC
EYDCVLQIGGGDQWGNIVSGVDLNRRVSGTKTHALTVPLVTDAQGQKFGKSTGGGKLWLD
CEEEEEEECCCCCCCCHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEC
PEKTSAYSWYQYFLNAGDSVVIDYLRWFTFLTQEEIAELETEVRERPHLRAAQRRLAQEM
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TDLVHGHDATVSVELAAQALFGRASLQDLDEATLDGALSETVIFEVPAGESPTIIDLLIG
HHHHCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHC
AGLVDSKGAARRTIKEGGAYVNNERIESEDWQPTDDVLLHGAWLVLRKGKKNFAGVKYSA
CCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHCEEEEEECCCCCCCCCEECC
>Mature Secondary Structure
MNIIDELQWRGLINQSTDIDALREACEKPITLYCGFDPTGDSLHAGHLVPMIMLRRFQEA
CCCHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GHRPITLAGGATGFIGDPRDVGERSMLSQDTIEHNLESIKKQLRSFVRFDGDSPAIMVNN
CCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
ADWTMSMSVVDFLRDVGKNFSLNTMLDRETVKRRLETDGISYTEFSYMLLQANDFVQLNR
CCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCEEEECC
EYDCVLQIGGGDQWGNIVSGVDLNRRVSGTKTHALTVPLVTDAQGQKFGKSTGGGKLWLD
CEEEEEEECCCCCCCCHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEC
PEKTSAYSWYQYFLNAGDSVVIDYLRWFTFLTQEEIAELETEVRERPHLRAAQRRLAQEM
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TDLVHGHDATVSVELAAQALFGRASLQDLDEATLDGALSETVIFEVPAGESPTIIDLLIG
HHHHCCCCCEEEHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHC
AGLVDSKGAARRTIKEGGAYVNNERIESEDWQPTDDVLLHGAWLVLRKGKKNFAGVKYSA
CCCCCCCCHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHCEEEEEECCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910