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The map label for this gene is polA [H]

Identifier: 38233736

GI number: 38233736

Start: 1129828

End: 1132527

Strand: Direct

Name: polA [H]

Synonym: DIP1146

Alternate gene names: 38233736

Gene position: 1129828-1132527 (Clockwise)

Preceding gene: 38233731

Following gene: 38233738

Centisome position: 45.4

GC content: 49.15

Gene sequence:

>2700_bases
ATGTTAGATGTACTCGTTACACTGGCCATCGTGACTTCCAATGCGAACCGACTCATGCTTATCGACGGCCACTCGATGGC
CTTCCGTGCGTTCTACGCGCTGCCTGCGGAGAACTTCTCTACATCGGGCGGACAGGCGACGAATGCCGTTTACGGATTTT
TGTCGATGTTGTCGTCTCTGCTCGTTGAAGAAAAGCCAACCCACGTAGCAGTTGCGTTCGACGTTGGCCGCCAAACATTT
CGAACCGAGATGTTCCCTGAATACAAAGCGCAGCGTGAGGCTGCGCCACCAGAATTCAAGGGACAGGTGGAAATTATTAA
AGAGGTCCTAGAGACTTTGGGAATCACCACCTTGGAAAAAGAAAATTTTGAGGCGGACGATATCATCGCCACGTTGGCTA
CCGCAGCGGGTCCACTTGGTTTTGATACGTATATCGTTACTGGTGATCGCGATTCTTTCCAGCTAGTAAACGAATCAACC
ACGGTGCTGTATCCCATGCGTGGTGTATCTGTTCTTCATCGTTTCACCCCAGAAGCGGTGGAAGAAAAATATGGATTAAC
TCCAGTACAGTATCCAGATTTCGCGGCATTGCGGGGAGATCCTTCAGATAATTTGCCTAATATCCCAGGTGTTGGTGAGA
AAACCGCTACAAAGTGGATCGTCCAATATGGAAATCTGGATTCCTTGCTTGCTCATGCCGATGAGATTAAGGGCAAAGCT
GGTAATAGCTTCCGAGAGCGTTTAGATCAGGTGCGTATGAATCGCACTTTGACTGAGATGATCAAGGATCTCGAACTTCC
TTATGCTCCAGATCAGCTCGAGCGCAAACCCGCTGATGCGTCGGCAATTGCATCTAAGTTTGATGAACTTGAATTTGGAT
CGAATCTCCGCGATCGAGTAATCCATGCAGTTGATGCGCAAGGGAACGTCTCTGAAGAGTCTGAAGAATACAAGCCTGAA
GTCGTCATCGACCATGAAAAACTAGCATCATGGCTTGCCACTCGTGCTGGGCAGTCACTGGCATTGTATGTGCGAGGCCA
TGGTAGTCCCGCCAGTGGTGATGCTGAGTCGGCGGCGATCGTCGATAAGCAATTCCATGCTGTAGCGTTTGATTTCGGTG
ACCTTGACGCCGATGACGACCAAGCATTTGCGCAGTGGATTGCTAGTGATTCGCCTAAATACCTACATGAAGCTAAAGCT
GTATTTCATATGCTCGCAGGCAGGGGCTACACTCTCAACGGCATCGAGCATGATACCGCAATTGCGGGGTACTTATTGCG
CCCTGGCCAGCGCACGTATGATCTGAAAGACGTGTATCAGCGCCATTTGCAACGTCAACTAGGCGGCGGTTCTAGCGAGA
GCGGTCAACTATCGTTGCTTGACGCACCTGATGCTCAAGAACTTGTTGACAGTGCTGTAGCGATTTTGGAGTTAAGTAAA
TCGCTTACTGCGCAACTACAAGCTATCGACGCATATGAGCTCTATCGGGAAATGGAGCTTCCGCTCGTTGGCGTGTTGGC
GCGCATGGAGGCAACTGGAATTTGCGTTGATGTGGCCACATTGCGGGAACAGCGAGACATATTTGTTGAGCAGGTTAAAG
AAGAAGAAAGCGCTGCCCGGGAACTCGCGGGGGACGAGACTCTAAATCTGTCGAGCCCAAAGCAGCTTCAAGTAGTTTTA
TTTGACACATTAGGGCTTCCTAAGACTAAGAAGACCAAGACTGGATACTCCACGGCAGCAAAAGAAATAGAATCGCTGGC
TGTTAAAAATCCACATCCGTTCCTTGATCATCTCTTGGCGCATCGAGAGTTCCAAAAGATGAAAACCACTCTAGACGGCC
TCATTAAAGCAGTCGGAGATGACGGTCGTATTCACACTACGTTTAATCAAACTGTCGCCTCAACTGGTCGTTTGAGTTCA
ACGGAACCAAACCTGCAAAACATTCCTGTGCGTACTCCAGCAGGAAGGAAAATTCGCTCTGCTTTTGTGGTAGGACAAGG
GTATAAGTCTCTGCTGACGGCAGACTATTCGCAGATTGAGATGCGTGTGATGGCGCATCTTTCTGAGGATCCAGGTTTGA
TTGAGGCTTATCAAACGGGTGAGGACCTCCACAATTTTGTGGGTTCGAAGGTCTTCGATGTGCCAGTTGATCAAGTAACT
CCTGAACTACGTCGCCGAGTTAAAGCGATGAGCTATGGGTTGGTCTATGGGCTTTCTGCATTTGGTCTTTCCCAACAATT
AAACATCCCTGCTGGTGAAGCCAAAGTGATCATGGAAAGCTACTTCGAACGATTCGGAGGGGTAAAACGATATCTTGACC
AAGTGGTCGAACAAGCCCGAAAGGATGGGTTCACATCGACTTTGTTTGGCCGTCGACGTTACTTGCCGGAGCTTTCTTCG
GATAATCGAGTGGCACGTGAAAATGCCGAACGAGCTGCATTGAATGCTCCAATTCAAGGCACAGCAGCAGACATTATTAA
GATTGCAATGCTGCGTGTCGACGCCCGCCTTACTGCAGAAAACTGCCAATCGAGGGTGCTTCTTCAAGTCCACGACGAAT
TGGTGCTCGAGGTAGCTTCAGGTGAACAAGAAAAGGTTCAACAACTTGTAGAAGAAGAAATGGACGCCGCTATTTCACTG
CGCGTACCGCTGGAGGTTTCTGCAGGTGTCGGTACAAACTGGGAAGAAGCTGCGCATTAA

Upstream 100 bases:

>100_bases
GTTTCCTCTGCCAATTGGGATACAGGGGCGCTGGAAAAAACTATAGACCATTAAGGTGGGTTTATGCCAAATTGCCTGTT
CGGAGGGTGGATTCGGGCAA

Downstream 100 bases:

>100_bases
TTTCAAGCTGGAATTGAGCCCGTGGCTGAGTGCGGAATTCATTCACGGGCTAACGGCAATGCGTTACAAAAATAGCTGTT
CCTGGAAAGATACGACCTCT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 899; Mature: 899

Protein sequence:

>899_residues
MLDVLVTLAIVTSNANRLMLIDGHSMAFRAFYALPAENFSTSGGQATNAVYGFLSMLSSLLVEEKPTHVAVAFDVGRQTF
RTEMFPEYKAQREAAPPEFKGQVEIIKEVLETLGITTLEKENFEADDIIATLATAAGPLGFDTYIVTGDRDSFQLVNEST
TVLYPMRGVSVLHRFTPEAVEEKYGLTPVQYPDFAALRGDPSDNLPNIPGVGEKTATKWIVQYGNLDSLLAHADEIKGKA
GNSFRERLDQVRMNRTLTEMIKDLELPYAPDQLERKPADASAIASKFDELEFGSNLRDRVIHAVDAQGNVSEESEEYKPE
VVIDHEKLASWLATRAGQSLALYVRGHGSPASGDAESAAIVDKQFHAVAFDFGDLDADDDQAFAQWIASDSPKYLHEAKA
VFHMLAGRGYTLNGIEHDTAIAGYLLRPGQRTYDLKDVYQRHLQRQLGGGSSESGQLSLLDAPDAQELVDSAVAILELSK
SLTAQLQAIDAYELYREMELPLVGVLARMEATGICVDVATLREQRDIFVEQVKEEESAARELAGDETLNLSSPKQLQVVL
FDTLGLPKTKKTKTGYSTAAKEIESLAVKNPHPFLDHLLAHREFQKMKTTLDGLIKAVGDDGRIHTTFNQTVASTGRLSS
TEPNLQNIPVRTPAGRKIRSAFVVGQGYKSLLTADYSQIEMRVMAHLSEDPGLIEAYQTGEDLHNFVGSKVFDVPVDQVT
PELRRRVKAMSYGLVYGLSAFGLSQQLNIPAGEAKVIMESYFERFGGVKRYLDQVVEQARKDGFTSTLFGRRRYLPELSS
DNRVARENAERAALNAPIQGTAADIIKIAMLRVDARLTAENCQSRVLLQVHDELVLEVASGEQEKVQQLVEEEMDAAISL
RVPLEVSAGVGTNWEEAAH

Sequences:

>Translated_899_residues
MLDVLVTLAIVTSNANRLMLIDGHSMAFRAFYALPAENFSTSGGQATNAVYGFLSMLSSLLVEEKPTHVAVAFDVGRQTF
RTEMFPEYKAQREAAPPEFKGQVEIIKEVLETLGITTLEKENFEADDIIATLATAAGPLGFDTYIVTGDRDSFQLVNEST
TVLYPMRGVSVLHRFTPEAVEEKYGLTPVQYPDFAALRGDPSDNLPNIPGVGEKTATKWIVQYGNLDSLLAHADEIKGKA
GNSFRERLDQVRMNRTLTEMIKDLELPYAPDQLERKPADASAIASKFDELEFGSNLRDRVIHAVDAQGNVSEESEEYKPE
VVIDHEKLASWLATRAGQSLALYVRGHGSPASGDAESAAIVDKQFHAVAFDFGDLDADDDQAFAQWIASDSPKYLHEAKA
VFHMLAGRGYTLNGIEHDTAIAGYLLRPGQRTYDLKDVYQRHLQRQLGGGSSESGQLSLLDAPDAQELVDSAVAILELSK
SLTAQLQAIDAYELYREMELPLVGVLARMEATGICVDVATLREQRDIFVEQVKEEESAARELAGDETLNLSSPKQLQVVL
FDTLGLPKTKKTKTGYSTAAKEIESLAVKNPHPFLDHLLAHREFQKMKTTLDGLIKAVGDDGRIHTTFNQTVASTGRLSS
TEPNLQNIPVRTPAGRKIRSAFVVGQGYKSLLTADYSQIEMRVMAHLSEDPGLIEAYQTGEDLHNFVGSKVFDVPVDQVT
PELRRRVKAMSYGLVYGLSAFGLSQQLNIPAGEAKVIMESYFERFGGVKRYLDQVVEQARKDGFTSTLFGRRRYLPELSS
DNRVARENAERAALNAPIQGTAADIIKIAMLRVDARLTAENCQSRVLLQVHDELVLEVASGEQEKVQQLVEEEMDAAISL
RVPLEVSAGVGTNWEEAAH
>Mature_899_residues
MLDVLVTLAIVTSNANRLMLIDGHSMAFRAFYALPAENFSTSGGQATNAVYGFLSMLSSLLVEEKPTHVAVAFDVGRQTF
RTEMFPEYKAQREAAPPEFKGQVEIIKEVLETLGITTLEKENFEADDIIATLATAAGPLGFDTYIVTGDRDSFQLVNEST
TVLYPMRGVSVLHRFTPEAVEEKYGLTPVQYPDFAALRGDPSDNLPNIPGVGEKTATKWIVQYGNLDSLLAHADEIKGKA
GNSFRERLDQVRMNRTLTEMIKDLELPYAPDQLERKPADASAIASKFDELEFGSNLRDRVIHAVDAQGNVSEESEEYKPE
VVIDHEKLASWLATRAGQSLALYVRGHGSPASGDAESAAIVDKQFHAVAFDFGDLDADDDQAFAQWIASDSPKYLHEAKA
VFHMLAGRGYTLNGIEHDTAIAGYLLRPGQRTYDLKDVYQRHLQRQLGGGSSESGQLSLLDAPDAQELVDSAVAILELSK
SLTAQLQAIDAYELYREMELPLVGVLARMEATGICVDVATLREQRDIFVEQVKEEESAARELAGDETLNLSSPKQLQVVL
FDTLGLPKTKKTKTGYSTAAKEIESLAVKNPHPFLDHLLAHREFQKMKTTLDGLIKAVGDDGRIHTTFNQTVASTGRLSS
TEPNLQNIPVRTPAGRKIRSAFVVGQGYKSLLTADYSQIEMRVMAHLSEDPGLIEAYQTGEDLHNFVGSKVFDVPVDQVT
PELRRRVKAMSYGLVYGLSAFGLSQQLNIPAGEAKVIMESYFERFGGVKRYLDQVVEQARKDGFTSTLFGRRRYLPELSS
DNRVARENAERAALNAPIQGTAADIIKIAMLRVDARLTAENCQSRVLLQVHDELVLEVASGEQEKVQQLVEEEMDAAISL
RVPLEVSAGVGTNWEEAAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=509, Percent_Identity=31.041257367387, Blast_Score=233, Evalue=5e-61,
Organism=Homo sapiens, GI139394648, Length=617, Percent_Identity=26.904376012966, Blast_Score=198, Evalue=2e-50,
Organism=Escherichia coli, GI1790294, Length=939, Percent_Identity=37.1671991480298, Blast_Score=524, Evalue=1e-149,
Organism=Escherichia coli, GI226510970, Length=224, Percent_Identity=24.5535714285714, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI86563272, Length=355, Percent_Identity=32.112676056338, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17933644, Length=532, Percent_Identity=29.3233082706767, Blast_Score=204, Evalue=2e-52,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 99020; Mature: 99020

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDVLVTLAIVTSNANRLMLIDGHSMAFRAFYALPAENFSTSGGQATNAVYGFLSMLSSL
CHHHHEEEHHEECCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LVEEKPTHVAVAFDVGRQTFRTEMFPEYKAQREAAPPEFKGQVEIIKEVLETLGITTLEK
HHCCCCCEEEEEEECCHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCEEECC
ENFEADDIIATLATAAGPLGFDTYIVTGDRDSFQLVNESTTVLYPMRGVSVLHRFTPEAV
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECCCCEEEEECHHHHHHHHCCHHHH
EEKYGLTPVQYPDFAALRGDPSDNLPNIPGVGEKTATKWIVQYGNLDSLLAHADEIKGKA
HHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
GNSFRERLDQVRMNRTLTEMIKDLELPYAPDQLERKPADASAIASKFDELEFGSNLRDRV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
IHAVDAQGNVSEESEEYKPEVVIDHEKLASWLATRAGQSLALYVRGHGSPASGDAESAAI
HHHCCCCCCCCCCHHHCCCCEEECHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHH
VDKQFHAVAFDFGDLDADDDQAFAQWIASDSPKYLHEAKAVFHMLAGRGYTLNGIEHDTA
HHHHHHEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHH
IAGYLLRPGQRTYDLKDVYQRHLQRQLGGGSSESGQLSLLDAPDAQELVDSAVAILELSK
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
SLTAQLQAIDAYELYREMELPLVGVLARMEATGICVDVATLREQRDIFVEQVKEEESAAR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ELAGDETLNLSSPKQLQVVLFDTLGLPKTKKTKTGYSTAAKEIESLAVKNPHPFLDHLLA
HHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
HREFQKMKTTLDGLIKAVGDDGRIHTTFNQTVASTGRLSSTEPNLQNIPVRTPAGRKIRS
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
AFVVGQGYKSLLTADYSQIEMRVMAHLSEDPGLIEAYQTGEDLHNFVGSKVFDVPVDQVT
HHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHCHHHHHHHHCCEEEECCHHHCC
PELRRRVKAMSYGLVYGLSAFGLSQQLNIPAGEAKVIMESYFERFGGVKRYLDQVVEQAR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
KDGFTSTLFGRRRYLPELSSDNRVARENAERAALNAPIQGTAADIIKIAMLRVDARLTAE
HCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
NCQSRVLLQVHDELVLEVASGEQEKVQQLVEEEMDAAISLRVPLEVSAGVGTNWEEAAH
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECEEECCCCCCCHHHHCC
>Mature Secondary Structure
MLDVLVTLAIVTSNANRLMLIDGHSMAFRAFYALPAENFSTSGGQATNAVYGFLSMLSSL
CHHHHEEEHHEECCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LVEEKPTHVAVAFDVGRQTFRTEMFPEYKAQREAAPPEFKGQVEIIKEVLETLGITTLEK
HHCCCCCEEEEEEECCHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCEEECC
ENFEADDIIATLATAAGPLGFDTYIVTGDRDSFQLVNESTTVLYPMRGVSVLHRFTPEAV
CCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEECCCCEEEEECHHHHHHHHCCHHHH
EEKYGLTPVQYPDFAALRGDPSDNLPNIPGVGEKTATKWIVQYGNLDSLLAHADEIKGKA
HHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCC
GNSFRERLDQVRMNRTLTEMIKDLELPYAPDQLERKPADASAIASKFDELEFGSNLRDRV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
IHAVDAQGNVSEESEEYKPEVVIDHEKLASWLATRAGQSLALYVRGHGSPASGDAESAAI
HHHCCCCCCCCCCHHHCCCCEEECHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHH
VDKQFHAVAFDFGDLDADDDQAFAQWIASDSPKYLHEAKAVFHMLAGRGYTLNGIEHDTA
HHHHHHEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHH
IAGYLLRPGQRTYDLKDVYQRHLQRQLGGGSSESGQLSLLDAPDAQELVDSAVAILELSK
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
SLTAQLQAIDAYELYREMELPLVGVLARMEATGICVDVATLREQRDIFVEQVKEEESAAR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ELAGDETLNLSSPKQLQVVLFDTLGLPKTKKTKTGYSTAAKEIESLAVKNPHPFLDHLLA
HHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
HREFQKMKTTLDGLIKAVGDDGRIHTTFNQTVASTGRLSSTEPNLQNIPVRTPAGRKIRS
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
AFVVGQGYKSLLTADYSQIEMRVMAHLSEDPGLIEAYQTGEDLHNFVGSKVFDVPVDQVT
HHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHCHHHHHHHHCCEEEECCHHHCC
PELRRRVKAMSYGLVYGLSAFGLSQQLNIPAGEAKVIMESYFERFGGVKRYLDQVVEQAR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
KDGFTSTLFGRRRYLPELSSDNRVARENAERAALNAPIQGTAADIIKIAMLRVDARLTAE
HCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
NCQSRVLLQVHDELVLEVASGEQEKVQQLVEEEMDAAISLRVPLEVSAGVGTNWEEAAH
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7476188; 11234002 [H]