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The map label for this gene is gatA

Identifier: 38233674

GI number: 38233674

Start: 1062018

End: 1063502

Strand: Direct

Name: gatA

Synonym: DIP1080

Alternate gene names: 38233674

Gene position: 1062018-1063502 (Clockwise)

Preceding gene: 38233673

Following gene: 38233676

Centisome position: 42.67

GC content: 53.13

Gene sequence:

>1485_bases
ATGAATAAATACCTCGTACCAGATTCCGGACTTACTTCCTTGTCCGCTGCCGAACTGGCAGAAAAAATCCACAGCCGCGA
AGTAACCTCTCGTGAAGTTACCCAAGCGCACTTGGATCGCATTAATGACGTTGATGGAACTCTCCATGCGTTTCTCCACG
TAGGTGTTGAGGAAGCACTGGCTGCAGCCGATGCCGTCGATGAGTCCTTGGATAAGGGAGAAGCCCCAGCATCAGCACTT
GCAGGTGTCCCACTAGCGTTGAAAGACGTCTTCGTTACTACCGACGCACCCACCACCTGTGCTTCCAAGATGCTCGAAGG
CTATGTTGCTCCTTACGACGCCACCGTTACCAAGAAGATCCGCGAGGCGGGAATTCCTATCTTGGGTAAGACCAACATGG
ACGAATTTGCCATGGGCTCTTCTACGGAAAACTCCGCCTATGGTCCAACATTGAACCCATGGGATATCGAGCGCACTCCA
GGCGGCTCCGGTGGCGGCACCTCAGCTGCACTTGCATCTGGCGAGGCGCCATTGGGTATCGGCACTGATACCGGTGGATC
GATTCGCCAGCCAGCAGCCTTGACTAACACTGTGGGTGTCAAACCAACCTATGGCACGGTTTCTCGCTACGGCCTAGTGG
CTTGTGCATCTTCTCTCGACCAAGGTGGCCCTACTGCGCGTACCGTTTTGGATACAGCATTGCTGCACGAAGTCATTGCT
GGTCACGATAAGTACGATGCAACCAGTGTTAATCGTGCGGTAGCTCCTGTGGTAGCTGCTGCACGCGAAGGTGCTCGCGG
TGATCTTAAAGGCACCAAGATCGGTGTGGTCAAGCAATTTGATCGTGAAGGTTACCAGCCAGGCGTTTTGGAGCAGTTCC
ACAAGTCTGTTGAGCAGCTTACTGCTCAGGGAGCAGAAATCGTTGAGGTTGATTGCCCTCACTTTGATGACGCATTGGCA
GCGTATTACTTGATTCTTCCATGTGAAGTTTCCTCCAACCTCGCGCGTTTTGACGGTATGCGTTACGGTTTGCGTGAGGG
CGATGACGGAACGCACTCTGCGGAAGAAGTCATGTCCTTGTCTCGTGCGGCAGGTTTCGGCCCAGAGGTTAAGCGTCGAA
TCATCTTGGGCACCTATGCCTTGTCTGTTGGCTACTACGATGCGTATTACTTGCAGGCACAGCGCGTACGTACCCTTATT
GCACAAGACTTCGCAGTGGCCTATGACAAGTGCGATGTGTTGGTCTCCCCAACGACTCCGACCACTGCTTTCAAGCTCGG
CGAGAAGGTATCTGATCCATTGGCTATGTACAACTTTGACTTGTGCACCTTGCCGCTCAACCTTGCTGGCTTGTGCGGTA
TGTCCTTGCCATCTGGTTTGGCACCTGATTCTCAGCTTCCAACTGGTTTGCAGATTATGGCTCCTGCCTTCCAAGACGAT
CGTCTTTACAAGGTAGGCGCGGCATTCGAGGTTGGTCAGAAGTAA

Upstream 100 bases:

>100_bases
TCGTTGAACAGATCCTCACTCCAGAACAGGCGCTTGATCAAGCACCAGCCGTCGACCAGCAGCGCTTCGTCGTGCCACAG
ATTTTGGGGGAGCAAGATTA

Downstream 100 bases:

>100_bases
TTTTTTAGTATTTCGTAGCTAGGAGTTCTTAATACTTAAAGGCAGCGCTCGTAAAAACTGTGAGCGCTGCCTTGTGGTAT
TTCCGCTAACAATAATGGTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNKYLVPDSGLTSLSAAELAEKIHSREVTSREVTQAHLDRINDVDGTLHAFLHVGVEEALAAADAVDESLDKGEAPASAL
AGVPLALKDVFVTTDAPTTCASKMLEGYVAPYDATVTKKIREAGIPILGKTNMDEFAMGSSTENSAYGPTLNPWDIERTP
GGSGGGTSAALASGEAPLGIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLVACASSLDQGGPTARTVLDTALLHEVIA
GHDKYDATSVNRAVAPVVAAAREGARGDLKGTKIGVVKQFDREGYQPGVLEQFHKSVEQLTAQGAEIVEVDCPHFDDALA
AYYLILPCEVSSNLARFDGMRYGLREGDDGTHSAEEVMSLSRAAGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLI
AQDFAVAYDKCDVLVSPTTPTTAFKLGEKVSDPLAMYNFDLCTLPLNLAGLCGMSLPSGLAPDSQLPTGLQIMAPAFQDD
RLYKVGAAFEVGQK

Sequences:

>Translated_494_residues
MNKYLVPDSGLTSLSAAELAEKIHSREVTSREVTQAHLDRINDVDGTLHAFLHVGVEEALAAADAVDESLDKGEAPASAL
AGVPLALKDVFVTTDAPTTCASKMLEGYVAPYDATVTKKIREAGIPILGKTNMDEFAMGSSTENSAYGPTLNPWDIERTP
GGSGGGTSAALASGEAPLGIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLVACASSLDQGGPTARTVLDTALLHEVIA
GHDKYDATSVNRAVAPVVAAAREGARGDLKGTKIGVVKQFDREGYQPGVLEQFHKSVEQLTAQGAEIVEVDCPHFDDALA
AYYLILPCEVSSNLARFDGMRYGLREGDDGTHSAEEVMSLSRAAGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLI
AQDFAVAYDKCDVLVSPTTPTTAFKLGEKVSDPLAMYNFDLCTLPLNLAGLCGMSLPSGLAPDSQLPTGLQIMAPAFQDD
RLYKVGAAFEVGQK
>Mature_494_residues
MNKYLVPDSGLTSLSAAELAEKIHSREVTSREVTQAHLDRINDVDGTLHAFLHVGVEEALAAADAVDESLDKGEAPASAL
AGVPLALKDVFVTTDAPTTCASKMLEGYVAPYDATVTKKIREAGIPILGKTNMDEFAMGSSTENSAYGPTLNPWDIERTP
GGSGGGTSAALASGEAPLGIGTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLVACASSLDQGGPTARTVLDTALLHEVIA
GHDKYDATSVNRAVAPVVAAAREGARGDLKGTKIGVVKQFDREGYQPGVLEQFHKSVEQLTAQGAEIVEVDCPHFDDALA
AYYLILPCEVSSNLARFDGMRYGLREGDDGTHSAEEVMSLSRAAGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLI
AQDFAVAYDKCDVLVSPTTPTTAFKLGEKVSDPLAMYNFDLCTLPLNLAGLCGMSLPSGLAPDSQLPTGLQIMAPAFQDD
RLYKVGAAFEVGQK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=34.0681362725451, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI195972892, Length=241, Percent_Identity=34.8547717842324, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI166795287, Length=214, Percent_Identity=32.2429906542056, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=418, Percent_Identity=36.8421052631579, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17537465, Length=256, Percent_Identity=30.46875, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=257, Percent_Identity=28.4046692607004, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=191, Percent_Identity=29.3193717277487, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=281, Percent_Identity=28.1138790035587, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=314, Percent_Identity=27.7070063694268, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=314, Percent_Identity=27.7070063694268, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=435, Percent_Identity=34.0229885057471, Blast_Score=226, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6319685, Length=420, Percent_Identity=29.2857142857143, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=202, Percent_Identity=26.2376237623762, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=459, Percent_Identity=40.7407407407407, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI24644968, Length=513, Percent_Identity=27.4853801169591, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550774, Length=209, Percent_Identity=33.9712918660287, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648435, Length=209, Percent_Identity=33.9712918660287, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648437, Length=209, Percent_Identity=33.9712918660287, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=25.5102040816327, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=25.5102040816327, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=25.5102040816327, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=25.5102040816327, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=234, Percent_Identity=31.1965811965812, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161078093, Length=517, Percent_Identity=24.9516441005803, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648441, Length=134, Percent_Identity=37.3134328358209, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648439, Length=134, Percent_Identity=37.3134328358209, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CORDI (Q6NHQ1)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939441.1
- ProteinModelPortal:   Q6NHQ1
- SMR:   Q6NHQ1
- GeneID:   2649340
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1080
- NMPDR:   fig|257309.1.peg.1032
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- PhylomeDB:   Q6NHQ1
- ProtClustDB:   PRK00012
- BioCyc:   CDIP257309:DIP1080-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52092; Mature: 52092

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 88-88 ACT_SITE 163-163 ACT_SITE 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYLVPDSGLTSLSAAELAEKIHSREVTSREVTQAHLDRINDVDGTLHAFLHVGVEEAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAADAVDESLDKGEAPASALAGVPLALKDVFVTTDAPTTCASKMLEGYVAPYDATVTKKI
HHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
REAGIPILGKTNMDEFAMGSSTENSAYGPTLNPWDIERTPGGSGGGTSAALASGEAPLGI
HHCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCC
GTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLVACASSLDQGGPTARTVLDTALLHEVIA
CCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GHDKYDATSVNRAVAPVVAAAREGARGDLKGTKIGVVKQFDREGYQPGVLEQFHKSVEQL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHH
TAQGAEIVEVDCPHFDDALAAYYLILPCEVSSNLARFDGMRYGLREGDDGTHSAEEVMSL
HHCCCEEEEECCCCCHHHHHHEEEEEECCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHH
SRAAGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLIAQDFAVAYDKCDVLVSPTTP
HHHCCCCHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
TTAFKLGEKVSDPLAMYNFDLCTLPLNLAGLCGMSLPSGLAPDSQLPTGLQIMAPAFQDD
HHHHHHHHHHCCCHHHEECCCEECCHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCC
RLYKVGAAFEVGQK
CEEEECCCCCCCCC
>Mature Secondary Structure
MNKYLVPDSGLTSLSAAELAEKIHSREVTSREVTQAHLDRINDVDGTLHAFLHVGVEEAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAADAVDESLDKGEAPASALAGVPLALKDVFVTTDAPTTCASKMLEGYVAPYDATVTKKI
HHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHH
REAGIPILGKTNMDEFAMGSSTENSAYGPTLNPWDIERTPGGSGGGTSAALASGEAPLGI
HHCCCCEEECCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCC
GTDTGGSIRQPAALTNTVGVKPTYGTVSRYGLVACASSLDQGGPTARTVLDTALLHEVIA
CCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GHDKYDATSVNRAVAPVVAAAREGARGDLKGTKIGVVKQFDREGYQPGVLEQFHKSVEQL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHH
TAQGAEIVEVDCPHFDDALAAYYLILPCEVSSNLARFDGMRYGLREGDDGTHSAEEVMSL
HHCCCEEEEECCCCCHHHHHHEEEEEECCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHH
SRAAGFGPEVKRRIILGTYALSVGYYDAYYLQAQRVRTLIAQDFAVAYDKCDVLVSPTTP
HHHCCCCHHHHHEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
TTAFKLGEKVSDPLAMYNFDLCTLPLNLAGLCGMSLPSGLAPDSQLPTGLQIMAPAFQDD
HHHHHHHHHHCCCHHHEECCCEECCHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCC
RLYKVGAAFEVGQK
CEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910