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The map label for this gene is ligA

Identifier: 38233671

GI number: 38233671

Start: 1058711

End: 1060744

Strand: Direct

Name: ligA

Synonym: DIP1077

Alternate gene names: 38233671

Gene position: 1058711-1060744 (Clockwise)

Preceding gene: 38233669

Following gene: 38233673

Centisome position: 42.54

GC content: 51.47

Gene sequence:

>2034_bases
GTGACTGATAATTTTGCTGATCTACGCCGCCAGTGGGACGACCTTGCCGAACAAGTTCGTCACCATAGAGATGCCTATTA
CAACCACACCCCTGAAATCTCAGACGCGGAGTTTGATCAGCTTTTCCGCCAGCTGCAGCAATTAGAACAGGAACATCCAG
AATTAGCTGTGCCAGAGAGCCCCACTTTGAGAGTCGGTGCTCCAGTTGAACAGAGTTCTTTCGACAATGTTGAGCACCTT
GAGCGCATGCTCAGTCTGGACAATGTTTTTGACGCTGCCGAGCTTGATGATTGGTTGCAGCGAACCCCCAGTGCTACGTA
TCTTACTGAGCTTAAAATCGATGGTCTGTCTATCGATTTGGTCTATCGTTCTGGGCGGCTTGAGCGTGCGGCTACTCGTG
GCGATGGTCGGGTAGGAGAAGATGTTACTGCTAACGCTAAAGTTATTGAGGACATCCCGCATCGTCTTCAGCATTCTGAT
GCATACCCAGTGCCAGAACTTGTAGAAATCCGTGGCGAGGTATTCATTGCCGTAGAAGATTTCGCTTTAGTCAATGAACA
ACGCCAAAAAGAAGGCGGAAAGCCGTTCGCAAACCCACGTAACGCCGCTGCTGGATCGCTGCGACAAAAAGATACTGAGG
CCGTTCGCAAGCGTCGTCTGAAGATGATTTGTCATGGAATCGGTGCTTCAGAAGGATTTGAAGCAGATACCCAGTTCGAT
GCATATAAAGCCCTAGAAGCATGGGGTCTGCCAGTATCGCCGTATACAAAACGTGTCCACTCGGCTCAAGAAGTTCAGGA
GCGCGTGACCTACTGGGCACAGCATCGCCACGATGCGACTCATGAGATGGACGGGTTAGTTATCAAGATCGATTCCTTTG
CAGAACAGCGCGCGTTAGGATCTACTGCCCGCGCGCCTCGCTGGGCAATCGCCTACAAGTATCCTCCAGAAGAAGTGACC
ACCAAGCTTCTTGATATTCAGGTCGGCGTTGGGCGTACCGGCAGGGTGACACCCTTTGCCGTTATGGACCCTGTGTTCGT
TGCTGGATCCACCGTAGAGATGGCTACGTTGCATAACCAGACCGAAGTAAAACGAAAAGGCGTTCTTATCGGCGATACCG
TGGTTATCCGCAAAGCCGGCGAAGTGATCCCAGAGGTTCTCGGTCCTGTTGTTGAAAAACGTGACGGTAGCGAACGCGAA
TTTATTTTCCCGACACTATGCCCAGCATGTGGAACACGCCTAGCGCCACAAAAAGAAGACGACGCCGACTGGCGATGCCC
GAACTCCCAATCATGTCCAGCGCAGTTGTCCTCACGTCTTACTTATCTAGCTGGACGAGGCGCATTTGATATCGAAGCCC
TCGGAGAAAAAGGAGCTGAGGATCTTATTGCCTCCGGCGTGCTTATCGACGAAGCCCAGCTGTTTAACCTGACGGAAGAC
GATCTTAAACGCACCAAGGTGTACACCACCAAAGCCGGTGCGCTTAACGCAACTGGTGAAAAGTTGCTGGCCAACCTTGA
GTCTGCAAAGCATACGGATCTGTGGCGTGTTCTCGTTGCACTGTCAATCCGACACGTTGGACCAACCGCAGCCAGAGCTC
TTGCGGTACGTTATCGAAGCCTCGAAGCATTACGGGCTGCTGATGTGGAGGACATTGCTAATACTGAGGGCGTTGGTGCG
ATTATCGCACAGAGCTTCGCGCAGTGGTTTGACGTGCCATGGCACAGAAATATCGTAGAAGTATGGGCTGATGCTGGTGT
CACAATGGCGGATTCGGAGGCAGATATCCCTGACCAAGTTCTAGAAGGATTGACCATTGTAGTGACCGGTTCTTTGGTCG
ATTTTTCTAGGGATTCCGCTAAAGAGGCAATTGTTTCTCGTGGTGGTAAAGCAAGCGGCTCGGTTTCCAAGAAGACCAGT
TACGTGGTAGTGGGTGAGAACGCAGGTAGCAAGGAAACGAAAGCCCGTGATCTGGGATTGCGTATCCTCAACGAAGACGA
GTTTAAACAGTTGTTAGCAAACGGCACCGTCTAG

Upstream 100 bases:

>100_bases
TCATAACGCTTACCCTAGAGAATAACCGTGACATGGGGGTAACTGGGCGTCGAAAAGCGTTGTTAACACGGCAATTGCCT
ACCCCGCTACACTGGGCTGC

Downstream 100 bases:

>100_bases
GACATAAAACAGTACGCCCTCCGAAGTGATGAAAAGTGCTTCGGAGGGCGTACTGAGTTGTTTTAGTGTTTGAGCAGGCT
GCCGAGGATCTTTCCTAGGT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MTDNFADLRRQWDDLAEQVRHHRDAYYNHTPEISDAEFDQLFRQLQQLEQEHPELAVPESPTLRVGAPVEQSSFDNVEHL
ERMLSLDNVFDAAELDDWLQRTPSATYLTELKIDGLSIDLVYRSGRLERAATRGDGRVGEDVTANAKVIEDIPHRLQHSD
AYPVPELVEIRGEVFIAVEDFALVNEQRQKEGGKPFANPRNAAAGSLRQKDTEAVRKRRLKMICHGIGASEGFEADTQFD
AYKALEAWGLPVSPYTKRVHSAQEVQERVTYWAQHRHDATHEMDGLVIKIDSFAEQRALGSTARAPRWAIAYKYPPEEVT
TKLLDIQVGVGRTGRVTPFAVMDPVFVAGSTVEMATLHNQTEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVEKRDGSERE
FIFPTLCPACGTRLAPQKEDDADWRCPNSQSCPAQLSSRLTYLAGRGAFDIEALGEKGAEDLIASGVLIDEAQLFNLTED
DLKRTKVYTTKAGALNATGEKLLANLESAKHTDLWRVLVALSIRHVGPTAARALAVRYRSLEALRAADVEDIANTEGVGA
IIAQSFAQWFDVPWHRNIVEVWADAGVTMADSEADIPDQVLEGLTIVVTGSLVDFSRDSAKEAIVSRGGKASGSVSKKTS
YVVVGENAGSKETKARDLGLRILNEDEFKQLLANGTV

Sequences:

>Translated_677_residues
MTDNFADLRRQWDDLAEQVRHHRDAYYNHTPEISDAEFDQLFRQLQQLEQEHPELAVPESPTLRVGAPVEQSSFDNVEHL
ERMLSLDNVFDAAELDDWLQRTPSATYLTELKIDGLSIDLVYRSGRLERAATRGDGRVGEDVTANAKVIEDIPHRLQHSD
AYPVPELVEIRGEVFIAVEDFALVNEQRQKEGGKPFANPRNAAAGSLRQKDTEAVRKRRLKMICHGIGASEGFEADTQFD
AYKALEAWGLPVSPYTKRVHSAQEVQERVTYWAQHRHDATHEMDGLVIKIDSFAEQRALGSTARAPRWAIAYKYPPEEVT
TKLLDIQVGVGRTGRVTPFAVMDPVFVAGSTVEMATLHNQTEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVEKRDGSERE
FIFPTLCPACGTRLAPQKEDDADWRCPNSQSCPAQLSSRLTYLAGRGAFDIEALGEKGAEDLIASGVLIDEAQLFNLTED
DLKRTKVYTTKAGALNATGEKLLANLESAKHTDLWRVLVALSIRHVGPTAARALAVRYRSLEALRAADVEDIANTEGVGA
IIAQSFAQWFDVPWHRNIVEVWADAGVTMADSEADIPDQVLEGLTIVVTGSLVDFSRDSAKEAIVSRGGKASGSVSKKTS
YVVVGENAGSKETKARDLGLRILNEDEFKQLLANGTV
>Mature_676_residues
TDNFADLRRQWDDLAEQVRHHRDAYYNHTPEISDAEFDQLFRQLQQLEQEHPELAVPESPTLRVGAPVEQSSFDNVEHLE
RMLSLDNVFDAAELDDWLQRTPSATYLTELKIDGLSIDLVYRSGRLERAATRGDGRVGEDVTANAKVIEDIPHRLQHSDA
YPVPELVEIRGEVFIAVEDFALVNEQRQKEGGKPFANPRNAAAGSLRQKDTEAVRKRRLKMICHGIGASEGFEADTQFDA
YKALEAWGLPVSPYTKRVHSAQEVQERVTYWAQHRHDATHEMDGLVIKIDSFAEQRALGSTARAPRWAIAYKYPPEEVTT
KLLDIQVGVGRTGRVTPFAVMDPVFVAGSTVEMATLHNQTEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVEKRDGSEREF
IFPTLCPACGTRLAPQKEDDADWRCPNSQSCPAQLSSRLTYLAGRGAFDIEALGEKGAEDLIASGVLIDEAQLFNLTEDD
LKRTKVYTTKAGALNATGEKLLANLESAKHTDLWRVLVALSIRHVGPTAARALAVRYRSLEALRAADVEDIANTEGVGAI
IAQSFAQWFDVPWHRNIVEVWADAGVTMADSEADIPDQVLEGLTIVVTGSLVDFSRDSAKEAIVSRGGKASGSVSKKTSY
VVVGENAGSKETKARDLGLRILNEDEFKQLLANGTV

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=76, Percent_Identity=47.3684210526316, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1788750, Length=682, Percent_Identity=41.2023460410557, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI87082305, Length=475, Percent_Identity=24.2105263157895, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CORDI (Q6NHQ4)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939438.1
- HSSP:   P63973
- ProteinModelPortal:   Q6NHQ4
- GeneID:   2649337
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1077
- NMPDR:   fig|257309.1.peg.1029
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q6NHQ4
- ProtClustDB:   PRK07956
- BioCyc:   CDIP257309:DIP1077-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74767; Mature: 74636

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 112-112 BINDING 110-110 BINDING 133-133 BINDING 173-173 BINDING 289-289 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNFADLRRQWDDLAEQVRHHRDAYYNHTPEISDAEFDQLFRQLQQLEQEHPELAVPES
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCC
PTLRVGAPVEQSSFDNVEHLERMLSLDNVFDAAELDDWLQRTPSATYLTELKIDGLSIDL
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEE
VYRSGRLERAATRGDGRVGEDVTANAKVIEDIPHRLQHSDAYPVPELVEIRGEVFIAVED
EEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEEH
FALVNEQRQKEGGKPFANPRNAAAGSLRQKDTEAVRKRRLKMICHGIGASEGFEADTQFD
HHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
AYKALEAWGLPVSPYTKRVHSAQEVQERVTYWAQHRHDATHEMDGLVIKIDSFAEQRALG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCHHHHHHCC
STARAPRWAIAYKYPPEEVTTKLLDIQVGVGRTGRVTPFAVMDPVFVAGSTVEMATLHNQ
CCCCCCCEEEEEECCHHHHHHHHHHEEECCCCCCCCCCHHHHCCHHHCCCCEEEHHHCCH
TEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVEKRDGSEREFIFPTLCPACGTRLAPQKED
HHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHCCCCCCCCEECHHHCHHHCCCCCCCCCC
DADWRCPNSQSCPAQLSSRLTYLAGRGAFDIEALGEKGAEDLIASGVLIDEAQLFNLTED
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHCCCCHH
DLKRTKVYTTKAGALNATGEKLLANLESAKHTDLWRVLVALSIRHVGPTAARALAVRYRS
HHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LEALRAADVEDIANTEGVGAIIAQSFAQWFDVPWHRNIVEVWADAGVTMADSEADIPDQV
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCHHHH
LEGLTIVVTGSLVDFSRDSAKEAIVSRGGKASGSVSKKTSYVVVGENAGSKETKARDLGL
HCCEEEEEECCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCC
RILNEDEFKQLLANGTV
EECCHHHHHHHHHCCCC
>Mature Secondary Structure 
TDNFADLRRQWDDLAEQVRHHRDAYYNHTPEISDAEFDQLFRQLQQLEQEHPELAVPES
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCC
PTLRVGAPVEQSSFDNVEHLERMLSLDNVFDAAELDDWLQRTPSATYLTELKIDGLSIDL
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEE
VYRSGRLERAATRGDGRVGEDVTANAKVIEDIPHRLQHSDAYPVPELVEIRGEVFIAVED
EEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEEH
FALVNEQRQKEGGKPFANPRNAAAGSLRQKDTEAVRKRRLKMICHGIGASEGFEADTQFD
HHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
AYKALEAWGLPVSPYTKRVHSAQEVQERVTYWAQHRHDATHEMDGLVIKIDSFAEQRALG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCHHHHHHCC
STARAPRWAIAYKYPPEEVTTKLLDIQVGVGRTGRVTPFAVMDPVFVAGSTVEMATLHNQ
CCCCCCCEEEEEECCHHHHHHHHHHEEECCCCCCCCCCHHHHCCHHHCCCCEEEHHHCCH
TEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVEKRDGSEREFIFPTLCPACGTRLAPQKED
HHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHCCCCCCCCEECHHHCHHHCCCCCCCCCC
DADWRCPNSQSCPAQLSSRLTYLAGRGAFDIEALGEKGAEDLIASGVLIDEAQLFNLTED
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHCCCCHH
DLKRTKVYTTKAGALNATGEKLLANLESAKHTDLWRVLVALSIRHVGPTAARALAVRYRS
HHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LEALRAADVEDIANTEGVGAIIAQSFAQWFDVPWHRNIVEVWADAGVTMADSEADIPDQV
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEECCCCCCHHHH
LEGLTIVVTGSLVDFSRDSAKEAIVSRGGKASGSVSKKTSYVVVGENAGSKETKARDLGL
HCCEEEEEECCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCC
RILNEDEFKQLLANGTV
EECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910