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The map label for this gene is aroE [C]

Identifier: 38233601

GI number: 38233601

Start: 979492

End: 980304

Strand: Reverse

Name: aroE [C]

Synonym: DIP1006

Alternate gene names: 38233601

Gene position: 980304-979492 (Counterclockwise)

Preceding gene: 38233605

Following gene: 38233600

Centisome position: 39.39

GC content: 52.15

Gene sequence:

>813_bases
ATGGTAAATCTCGTCGACCGAAATACCGTTTTGTGTGTCTCATTGGCCGCACGGCCCTCAAATCATGGGGTGCGCTATCA
TAATTGGCTATATGACCAGCTGGGATTGAACTTTATCTATAAGGCAATCGCACCGGCAGATATTACTGCTGCCGTGGCTG
GAATTCGTGGATTGAATATTCGTGGCGCCGGTGTGTCCATGCCCTATAAGCAGGACGTTATCCCGCTGATAGATGCGCTT
CACCCCTCGGCACAACGCATTCATGCTGTGAACACGATTGTTAATGACGACGGTGTACTCACCGGATATAACACCGATTA
TGTCGCAGTTGCCTCGTTGCTTAAGTCCCATGAGATTCCCGCTGATATGAGCGTTGCAGTCCGTGGTTCAGGTGGCATGG
CCAATGCGGTTGTGGCAGCACTAGCTGACCATGGCATGACGGGCACTGTGGTTGCGCGCAATGCAGAAACTGGTTCCGCA
TTGGCACAGCGCTATGGTTGGGAGCATTCCACCGAGGTACCGGCTAAAACCAGCATGTTGGTTAATGTGACTCCCCTTGG
CATGTGCGGCGATCATGAACATGTGCAGGCGTTTAGCAATGCCGAGATCGATGCTGCGCAGGTGATCTTCGATGTCGTTG
CCTATCCGGTAGAAACTCCACTGATCAAGGCAGCTGAGTCTAAGGGCAAAAAGATTATTAACGGTGGTGAGGTGATCTGT
TTGCAGGCTGCTGAGCAGTTTGCGCTCTACACCGGAGTAACTCCTTCTGCTGCGTTTGTGCGCGCGGCAGAAGATTACGC
ACAGCAGGGTTAA

Upstream 100 bases:

>100_bases
GGTGTCGGTGGCTGTGGTGCTTAACCCTCCTACTCGGCCAGACGTGGTACGTACTACGTCGTTGGGGGTCCAATCACTCA
TATGTTTTAGAGTAGGTGAC

Downstream 100 bases:

>100_bases
CCGGCTCTTCCGGTTTTAGAGTGCATTGATTAGCTCGCTGGGGGTTCGGGCGTGGCACAAGATACTAGGGTCCTTGGCTT
TGTCCGTACAAAACCAAGGA

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MVNLVDRNTVLCVSLAARPSNHGVRYHNWLYDQLGLNFIYKAIAPADITAAVAGIRGLNIRGAGVSMPYKQDVIPLIDAL
HPSAQRIHAVNTIVNDDGVLTGYNTDYVAVASLLKSHEIPADMSVAVRGSGGMANAVVAALADHGMTGTVVARNAETGSA
LAQRYGWEHSTEVPAKTSMLVNVTPLGMCGDHEHVQAFSNAEIDAAQVIFDVVAYPVETPLIKAAESKGKKIINGGEVIC
LQAAEQFALYTGVTPSAAFVRAAEDYAQQG

Sequences:

>Translated_270_residues
MVNLVDRNTVLCVSLAARPSNHGVRYHNWLYDQLGLNFIYKAIAPADITAAVAGIRGLNIRGAGVSMPYKQDVIPLIDAL
HPSAQRIHAVNTIVNDDGVLTGYNTDYVAVASLLKSHEIPADMSVAVRGSGGMANAVVAALADHGMTGTVVARNAETGSA
LAQRYGWEHSTEVPAKTSMLVNVTPLGMCGDHEHVQAFSNAEIDAAQVIFDVVAYPVETPLIKAAESKGKKIINGGEVIC
LQAAEQFALYTGVTPSAAFVRAAEDYAQQG
>Mature_270_residues
MVNLVDRNTVLCVSLAARPSNHGVRYHNWLYDQLGLNFIYKAIAPADITAAVAGIRGLNIRGAGVSMPYKQDVIPLIDAL
HPSAQRIHAVNTIVNDDGVLTGYNTDYVAVASLLKSHEIPADMSVAVRGSGGMANAVVAALADHGMTGTVVARNAETGSA
LAQRYGWEHSTEVPAKTSMLVNVTPLGMCGDHEHVQAFSNAEIDAAQVIFDVVAYPVETPLIKAAESKGKKIINGGEVIC
LQAAEQFALYTGVTPSAAFVRAAEDYAQQG

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=272, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789675, Length=243, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 28525; Mature: 28525

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLVDRNTVLCVSLAARPSNHGVRYHNWLYDQLGLNFIYKAIAPADITAAVAGIRGLNI
CCEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
RGAGVSMPYKQDVIPLIDALHPSAQRIHAVNTIVNDDGVLTGYNTDYVAVASLLKSHEIP
ECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCEEECCCCCHHHHHHHHHHCCCC
ADMSVAVRGSGGMANAVVAALADHGMTGTVVARNAETGSALAQRYGWEHSTEVPAKTSML
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEE
VNVTPLGMCGDHEHVQAFSNAEIDAAQVIFDVVAYPVETPLIKAAESKGKKIINGGEVIC
EEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCEEE
LQAAEQFALYTGVTPSAAFVRAAEDYAQQG
EECHHHHHEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVNLVDRNTVLCVSLAARPSNHGVRYHNWLYDQLGLNFIYKAIAPADITAAVAGIRGLNI
CCEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
RGAGVSMPYKQDVIPLIDALHPSAQRIHAVNTIVNDDGVLTGYNTDYVAVASLLKSHEIP
ECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCEEECCCCCHHHHHHHHHHCCCC
ADMSVAVRGSGGMANAVVAALADHGMTGTVVARNAETGSALAQRYGWEHSTEVPAKTSML
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEE
VNVTPLGMCGDHEHVQAFSNAEIDAAQVIFDVVAYPVETPLIKAAESKGKKIINGGEVIC
EEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCEEE
LQAAEQFALYTGVTPSAAFVRAAEDYAQQG
EECHHHHHEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]