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The map label for this gene is folP [H]

Identifier: 38233579

GI number: 38233579

Start: 955524

End: 956381

Strand: Direct

Name: folP [H]

Synonym: DIP0984

Alternate gene names: 38233579

Gene position: 955524-956381 (Clockwise)

Preceding gene: 38233578

Following gene: 38233580

Centisome position: 38.4

GC content: 58.39

Gene sequence:

>858_bases
ATGACTAGGAGTGCGTTTCAACACGATCCCGAGTGCGCAACGGTCATGGCGATCGTTAATCGCACCCCAGATTCCTTTTA
CGACCACGGTGCGACCCATGCGTTAGCAGATGCCATAACACGCGCCAGTGCTGTGATTGCGCAGGGGGCGGGGATCGTCG
ATATTGGTGGGGTCAAGGCAGGTCCTGGCCCTTATGTCTCTCCTAGTGAGGAGATTGAACGGGTGGTACCAGTTATCGAC
GCAGTTCACCGTGCGCACCCCGAGATCACCATTTCGGTAGATACGTGGCGTGCCGAGGTCGCTGATGTGGCGATCCGCGC
CGGGGCGTCGTTAGTAAACGACACGTGGGCCGGCCATGATCCGGAGCTTGCCGAGGTAGCGGGACATTATCGCGTGGGAT
ACGTGTGTTCTCATACGGGAGGCGTAGCGCCACGCACCCGCCCGCACCGCGTGCGTTTCGACGACATCGTTGCCTCTGTT
ATTGAGGAAACAACAGCTCAAGCTGAGCGGGCGGTTCGCCTTGGTGTTCCTGAGGACCGCATTCTTATCGACCCCACCCA
TGATTTTGGCAAAAACACCTTTCATGGACTGGAGCTGCTGCGGCGTATCGATGAGATTGTGGCAACCGGCTGGCCAGTAC
TGATGGCCTTGTCTAACAAAGATTTTGTAGGAGAGACGCTGGCTCGCTCTGTCGACCAGCGTGTAGCCGGAACCCTTGCA
GCTACAGCGTGGGCAGCGGCTCATGGAGTTGCGGCTTTTCGTTCGCACCAAGTCGCAGAAACCGTCGATGTGATCCGCAT
GACCGCCGCAATCCAAGGACGCGTGCCACCTCTGGCTACCACACGGGGGCTTGTATGA

Upstream 100 bases:

>100_bases
ATGATTTCCGAGGAAGATTTGTCCTTGTTCTTAGTCACTGATTCGGTCGACGAGGCGGTGGAGTTCATTCGCCAAGCGCA
CCGCAGAATGATGCACGTTG

Downstream 100 bases:

>100_bases
ACGTCTCCGTAGTAATTCCTGCCCTCAACGAGGAAGCAACCGTGGCCGATGTGGTCGCATTGTCTAGGAGGGGGAGTGAC
GAGGTCATCGTCATCGACTC

Product: dihydropteroate synthase

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2 [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTRSAFQHDPECATVMAIVNRTPDSFYDHGATHALADAITRASAVIAQGAGIVDIGGVKAGPGPYVSPSEEIERVVPVID
AVHRAHPEITISVDTWRAEVADVAIRAGASLVNDTWAGHDPELAEVAGHYRVGYVCSHTGGVAPRTRPHRVRFDDIVASV
IEETTAQAERAVRLGVPEDRILIDPTHDFGKNTFHGLELLRRIDEIVATGWPVLMALSNKDFVGETLARSVDQRVAGTLA
ATAWAAAHGVAAFRSHQVAETVDVIRMTAAIQGRVPPLATTRGLV

Sequences:

>Translated_285_residues
MTRSAFQHDPECATVMAIVNRTPDSFYDHGATHALADAITRASAVIAQGAGIVDIGGVKAGPGPYVSPSEEIERVVPVID
AVHRAHPEITISVDTWRAEVADVAIRAGASLVNDTWAGHDPELAEVAGHYRVGYVCSHTGGVAPRTRPHRVRFDDIVASV
IEETTAQAERAVRLGVPEDRILIDPTHDFGKNTFHGLELLRRIDEIVATGWPVLMALSNKDFVGETLARSVDQRVAGTLA
ATAWAAAHGVAAFRSHQVAETVDVIRMTAAIQGRVPPLATTRGLV
>Mature_284_residues
TRSAFQHDPECATVMAIVNRTPDSFYDHGATHALADAITRASAVIAQGAGIVDIGGVKAGPGPYVSPSEEIERVVPVIDA
VHRAHPEITISVDTWRAEVADVAIRAGASLVNDTWAGHDPELAEVAGHYRVGYVCSHTGGVAPRTRPHRVRFDDIVASVI
EETTAQAERAVRLGVPEDRILIDPTHDFGKNTFHGLELLRRIDEIVATGWPVLMALSNKDFVGETLARSVDQRVAGTLAA
TAWAAAHGVAAFRSHQVAETVDVIRMTAAIQGRVPPLATTRGLV

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism [H]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=257, Percent_Identity=35.7976653696498, Blast_Score=156, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324073, Length=315, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489 [H]

Pfam domain/function: PF00809 Pterin_bind [H]

EC number: 2.5.1.15

Molecular weight: Translated: 30444; Mature: 30312

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00793 DHPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSAFQHDPECATVMAIVNRTPDSFYDHGATHALADAITRASAVIAQGAGIVDIGGVKA
CCCCCCCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
GPGPYVSPSEEIERVVPVIDAVHRAHPEITISVDTWRAEVADVAIRAGASLVNDTWAGHD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCHHHHCCCCCCC
PELAEVAGHYRVGYVCSHTGGVAPRTRPHRVRFDDIVASVIEETTAQAERAVRLGVPEDR
CHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCC
ILIDPTHDFGKNTFHGLELLRRIDEIVATGWPVLMALSNKDFVGETLARSVDQRVAGTLA
EEECCCHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
ATAWAAAHGVAAFRSHQVAETVDVIRMTAAIQGRVPPLATTRGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TRSAFQHDPECATVMAIVNRTPDSFYDHGATHALADAITRASAVIAQGAGIVDIGGVKA
CCCCCCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
GPGPYVSPSEEIERVVPVIDAVHRAHPEITISVDTWRAEVADVAIRAGASLVNDTWAGHD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCHHHHCCCCCCC
PELAEVAGHYRVGYVCSHTGGVAPRTRPHRVRFDDIVASVIEETTAQAERAVRLGVPEDR
CHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCC
ILIDPTHDFGKNTFHGLELLRRIDEIVATGWPVLMALSNKDFVGETLARSVDQRVAGTLA
EEECCCHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
ATAWAAAHGVAAFRSHQVAETVDVIRMTAAIQGRVPPLATTRGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]