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The map label for this gene is eno [H]

Identifier: 38233513

GI number: 38233513

Start: 891487

End: 892764

Strand: Direct

Name: eno [H]

Synonym: DIP0917

Alternate gene names: 38233513

Gene position: 891487-892764 (Clockwise)

Preceding gene: 38233512

Following gene: 38233514

Centisome position: 35.82

GC content: 57.28

Gene sequence:

>1278_bases
GTGGCTGACATTATGCACGTATTTGCTCGTGAAATCTTGGACTCCCGCGGTAACCCAACCGTTGAAGCCGAAGTTTTCTT
GGATGACGGATCCCACGGCGTTGCGGGCGTTCCTTCCGGTGCGTCCACCGGTGTTCACGAGGCTCACGAGCTTCGCGACG
GTGGCGAGCGCTACCTCGGCAAGGGCGTTCTCAATGCAGTTAACAACGTGAACGAGGAAATCGCTGACGCAATCGCTGGT
GCAGAAGCCGACGATCAGCGCCTGATCGATCAGGCGATGATTGCTCTCGACGGCACTGAGAACAAGTCTCGTCTCGGCGC
AAACGCTATCTTGGGTGTATCCATCGCCGTAGCTAAGGCTGCCGCAGAGTCTGCTGGCCTACCTTTGTACCGCTACATCG
GCGGCCCTAACGCTCACGTTCTTCCAGTTCCTATGATGAACATTGTTAACGGTGGCGCACACGCTGACTCCGGCGTTGAT
GTTCAGGAGTTCATGATTGCTCCTATCGGTGCCGAGTCCTTCTCTGAGGCTCTGCGCATGGGTGCAGAGGTCTACCACTC
CTTGAAGTCCGTGATTAAGTCCAAGGGACTTTCCACCGGCCTCGGCGACGAAGGTGGTTTCGCACCTTCTGTTGAGTCCA
CCAAGGCAGCTCTCGACCTCATCGTTGAGGCAATTGAGAAGGCTGGCTTCAAGCCAGGTGCTGACATCGCACTTGCACTC
GACGTTGCTTCCTCCGAGTTCTACAAGGATGGCAAGTACCACTTCGAGGGGGGCGAGCACACCGCTGAGGAGATGGCAAA
GGTCTACGAGCAGCTCATCGCTGAGTACCCAATTGTTTCCATCGAGGACCCACTGCAGGAAGACGACTGGGAGGGCTACA
CCGCCCTGACCGCCGCAATCGGTGACAAGGTTCAGATCGTCGGCGACGACTTCTTCGTCACCAACCCAGCACGCCTCAAG
GAAGGCATCGAGAAGAAGGCTGCCAATGCCTTGCTGGTAAAGGTCAACCAGATCGGTACCCTGACCGAGACCTTCGACGC
TGTTGATCTCGCACACCGCAACGGCTACCGCACCATGATGTCCCACCGCTCCGGCGAGACTGAAGACACCACCATTGCTG
ACCTTGCAGTCGCATTGGGCTGTGGCCAGATCAAGACCGGTGCACCAGCTCGTTCCGAGCGCGTTGCCAAGTACAATCAG
CTTCTGCGCATCGAGCAGCAGCTTGACGATGCAGCAGTCTACGCAGGCCGTTCAGCATTTCCACGTTTTCAGGGCTAA

Upstream 100 bases:

>100_bases
AACGGACAAAGTAGTTTTTGTTTGTTCAACATTTTTAGCGTGTGTAACTTGCAATGGATGAATAATCTTGCATCCACACA
ACGTCAATAGGAGACACACA

Downstream 100 bases:

>100_bases
TAACAACTCGTAATTAACCCCGAAACACGTTTCAGTCCCCACGAAGATATTTTTATCTCCGTGGGGACTGAAATTTTCTT
CACCACCCTTGATTGACAAC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MADIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGERYLGKGVLNAVNNVNEEIADAIAG
AEADDQRLIDQAMIALDGTENKSRLGANAILGVSIAVAKAAAESAGLPLYRYIGGPNAHVLPVPMMNIVNGGAHADSGVD
VQEFMIAPIGAESFSEALRMGAEVYHSLKSVIKSKGLSTGLGDEGGFAPSVESTKAALDLIVEAIEKAGFKPGADIALAL
DVASSEFYKDGKYHFEGGEHTAEEMAKVYEQLIAEYPIVSIEDPLQEDDWEGYTALTAAIGDKVQIVGDDFFVTNPARLK
EGIEKKAANALLVKVNQIGTLTETFDAVDLAHRNGYRTMMSHRSGETEDTTIADLAVALGCGQIKTGAPARSERVAKYNQ
LLRIEQQLDDAAVYAGRSAFPRFQG

Sequences:

>Translated_425_residues
MADIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGERYLGKGVLNAVNNVNEEIADAIAG
AEADDQRLIDQAMIALDGTENKSRLGANAILGVSIAVAKAAAESAGLPLYRYIGGPNAHVLPVPMMNIVNGGAHADSGVD
VQEFMIAPIGAESFSEALRMGAEVYHSLKSVIKSKGLSTGLGDEGGFAPSVESTKAALDLIVEAIEKAGFKPGADIALAL
DVASSEFYKDGKYHFEGGEHTAEEMAKVYEQLIAEYPIVSIEDPLQEDDWEGYTALTAAIGDKVQIVGDDFFVTNPARLK
EGIEKKAANALLVKVNQIGTLTETFDAVDLAHRNGYRTMMSHRSGETEDTTIADLAVALGCGQIKTGAPARSERVAKYNQ
LLRIEQQLDDAAVYAGRSAFPRFQG
>Mature_424_residues
ADIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGERYLGKGVLNAVNNVNEEIADAIAGA
EADDQRLIDQAMIALDGTENKSRLGANAILGVSIAVAKAAAESAGLPLYRYIGGPNAHVLPVPMMNIVNGGAHADSGVDV
QEFMIAPIGAESFSEALRMGAEVYHSLKSVIKSKGLSTGLGDEGGFAPSVESTKAALDLIVEAIEKAGFKPGADIALALD
VASSEFYKDGKYHFEGGEHTAEEMAKVYEQLIAEYPIVSIEDPLQEDDWEGYTALTAAIGDKVQIVGDDFFVTNPARLKE
GIEKKAANALLVKVNQIGTLTETFDAVDLAHRNGYRTMMSHRSGETEDTTIADLAVALGCGQIKTGAPARSERVAKYNQL
LRIEQQLDDAAVYAGRSAFPRFQG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=54.1860465116279, Blast_Score=450, Evalue=1e-127,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=54.1860465116279, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=54.1860465116279, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=52.093023255814, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=49.5327102803738, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=27.1641791044776, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=27.1641791044776, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=27.1641791044776, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=58.3529411764706, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=54.9883990719258, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=54.9883990719258, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=52.3560209424084, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=54.0415704387991, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=51.7401392111369, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=51.7401392111369, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=51.2761020881671, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=54.5034642032333, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=54.292343387471, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=54.292343387471, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=54.292343387471, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=54.292343387471, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=54.292343387471, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=54.292343387471, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45030; Mature: 44899

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGERYLG
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
KGVLNAVNNVNEEIADAIAGAEADDQRLIDQAMIALDGTENKSRLGANAILGVSIAVAKA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHH
AAESAGLPLYRYIGGPNAHVLPVPMMNIVNGGAHADSGVDVQEFMIAPIGAESFSEALRM
HHHHCCCCEEEECCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHH
GAEVYHSLKSVIKSKGLSTGLGDEGGFAPSVESTKAALDLIVEAIEKAGFKPGADIALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DVASSEFYKDGKYHFEGGEHTAEEMAKVYEQLIAEYPIVSIEDPLQEDDWEGYTALTAAI
EEHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHH
GDKVQIVGDDFFVTNPARLKEGIEKKAANALLVKVNQIGTLTETFDAVDLAHRNGYRTMM
CCEEEEEECCEEECCHHHHHHHHHHHHCCEEEEEEHHCCCHHHHHHHHHHHHCCCHHHHH
SHRSGETEDTTIADLAVALGCGQIKTGAPARSERVAKYNQLLRIEQQLDDAAVYAGRSAF
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRFQG
CCCCC
>Mature Secondary Structure 
ADIMHVFAREILDSRGNPTVEAEVFLDDGSHGVAGVPSGASTGVHEAHELRDGGERYLG
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
KGVLNAVNNVNEEIADAIAGAEADDQRLIDQAMIALDGTENKSRLGANAILGVSIAVAKA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHH
AAESAGLPLYRYIGGPNAHVLPVPMMNIVNGGAHADSGVDVQEFMIAPIGAESFSEALRM
HHHHCCCCEEEECCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHH
GAEVYHSLKSVIKSKGLSTGLGDEGGFAPSVESTKAALDLIVEAIEKAGFKPGADIALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DVASSEFYKDGKYHFEGGEHTAEEMAKVYEQLIAEYPIVSIEDPLQEDDWEGYTALTAAI
EEHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHH
GDKVQIVGDDFFVTNPARLKEGIEKKAANALLVKVNQIGTLTETFDAVDLAHRNGYRTMM
CCEEEEEECCEEECCHHHHHHHHHHHHCCEEEEEEHHCCCHHHHHHHHHHHHCCCHHHHH
SHRSGETEDTTIADLAVALGCGQIKTGAPARSERVAKYNQLLRIEQQLDDAAVYAGRSAF
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PRFQG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA