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The map label for this gene is paaF [C]

Identifier: 38233482

GI number: 38233482

Start: 858962

End: 860014

Strand: Direct

Name: paaF [C]

Synonym: DIP0885

Alternate gene names: 38233482

Gene position: 858962-860014 (Clockwise)

Preceding gene: 38233479

Following gene: 38233489

Centisome position: 34.52

GC content: 55.65

Gene sequence:

>1053_bases
ATGAATAACAAAACCTCAGCAGCGCCCGTACGCGCCTACGTAGAAAACTCCACAGGAGTGTTAGAACTCAACCGCCCCCA
AGCACTCAACTCGCTAACTCACGAGATGGTCAACATCATCGACCAAGCGCTCGATCAATGGTGGGACGACGACGCAGTCC
ACCGAGTGCTCGTTTATTCCAATTCGCCCAAAGCCTTTTGCGCAGGTGGCGACGTCCGCGCAGCTCGAGAAGCAATCCAA
GCCGGCCGCGGTGGGGAAGCAGACCAATTCTTCGTCGACGAATACGACATGAATAACAACATCTCCGAGTTCCCCAAGCC
CTACATTTCGCTTATCGACGGCTTCGTCATGGGCGGTGGACTCGGACTTTCTGCCCACGGATCTCACCGAGTAATTAGCG
AAAAAGCCAGTGCCGCCATGCCCGAAATGGCTATCGGATTCACCCCCGATGTCGGTATGGCCTACATGTTCCAACGAATG
ACCAACGCAACAGGAAGTACCTCCCACGCGCTCGCCGCCTTCCTCGTGACCACCGGATGGCGTATGTCAGCCGCCGATAT
GATCTGGTCCGGACTAGCGACCGATATAGTCCCCAGCGCTGACCACGACCTCTTCCGCGAAACTGTCTACGCAGAATCTC
TCGATGAAGCACTCGAACGCTACAGTGTAGACACTGTCGGGGACAGCGAACTCGCGACACTGCTACCGCACATCGAGAAA
ACCTTCGGTTTTCCCACGTGGGCACAAATCAACGAAGCACTCGACACCTACCCAGACCGCGAGTTCGTCAACACCGTAAC
CGAGCTACTTAGCACTGCAAACCCAGAATCACTCGTCGCAGCAGTAGAACTCATGGTTGCTTCTGCACGCAGTACCACCT
TGCGCGAAGAACTCGATCACGAAGTCGTACTCGGGGAATACATCCGCAGCCGACCCAACTTCGCTGAAGGAGTACGCGCA
GTCCTCGTAGACAAAGACCGCAACGCCGACTTCACACCAGCAACCACTGGGGAAGTCGACGTCACGCCCATGAGGAAACT
TCTCAAGAACTAG

Upstream 100 bases:

>100_bases
TAAGAAACCACTTTACCTATCTGCGTGGGGACTCGAGCGTTCGCCGTCCGGTGTCGGAAACCACATGCACAATAGCGGGT
GATGTGGAACACTGGCGGAC

Downstream 100 bases:

>100_bases
ACGATGCTGCACACCCTGCCACATGCGTCCATCGATACCTGAATAGGTGCCGATGGCGCATGCACGCGTACCCTACGATA
ATCCACATCATCGATAGTGA

Product: 3-hydroxyisobutyryl-CoA hydrolase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNNKTSAAPVRAYVENSTGVLELNRPQALNSLTHEMVNIIDQALDQWWDDDAVHRVLVYSNSPKAFCAGGDVRAAREAIQ
AGRGGEADQFFVDEYDMNNNISEFPKPYISLIDGFVMGGGLGLSAHGSHRVISEKASAAMPEMAIGFTPDVGMAYMFQRM
TNATGSTSHALAAFLVTTGWRMSAADMIWSGLATDIVPSADHDLFRETVYAESLDEALERYSVDTVGDSELATLLPHIEK
TFGFPTWAQINEALDTYPDREFVNTVTELLSTANPESLVAAVELMVASARSTTLREELDHEVVLGEYIRSRPNFAEGVRA
VLVDKDRNADFTPATTGEVDVTPMRKLLKN

Sequences:

>Translated_350_residues
MNNKTSAAPVRAYVENSTGVLELNRPQALNSLTHEMVNIIDQALDQWWDDDAVHRVLVYSNSPKAFCAGGDVRAAREAIQ
AGRGGEADQFFVDEYDMNNNISEFPKPYISLIDGFVMGGGLGLSAHGSHRVISEKASAAMPEMAIGFTPDVGMAYMFQRM
TNATGSTSHALAAFLVTTGWRMSAADMIWSGLATDIVPSADHDLFRETVYAESLDEALERYSVDTVGDSELATLLPHIEK
TFGFPTWAQINEALDTYPDREFVNTVTELLSTANPESLVAAVELMVASARSTTLREELDHEVVLGEYIRSRPNFAEGVRA
VLVDKDRNADFTPATTGEVDVTPMRKLLKN
>Mature_350_residues
MNNKTSAAPVRAYVENSTGVLELNRPQALNSLTHEMVNIIDQALDQWWDDDAVHRVLVYSNSPKAFCAGGDVRAAREAIQ
AGRGGEADQFFVDEYDMNNNISEFPKPYISLIDGFVMGGGLGLSAHGSHRVISEKASAAMPEMAIGFTPDVGMAYMFQRM
TNATGSTSHALAAFLVTTGWRMSAADMIWSGLATDIVPSADHDLFRETVYAESLDEALERYSVDTVGDSELATLLPHIEK
TFGFPTWAQINEALDTYPDREFVNTVTELLSTANPESLVAAVELMVASARSTTLREELDHEVVLGEYIRSRPNFAEGVRA
VLVDKDRNADFTPATTGEVDVTPMRKLLKN

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=351, Percent_Identity=36.4672364672365, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI37594469, Length=318, Percent_Identity=35.5345911949685, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194097323, Length=188, Percent_Identity=32.9787234042553, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1787659, Length=204, Percent_Identity=27.4509803921569, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=333, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25144160, Length=333, Percent_Identity=33.3333333333333, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17540714, Length=191, Percent_Identity=28.7958115183246, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=188, Percent_Identity=28.1914893617021, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=160, Percent_Identity=31.25, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=201, Percent_Identity=27.8606965174129, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=354, Percent_Identity=29.9435028248588, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI28571729, Length=346, Percent_Identity=35.5491329479769, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28571730, Length=346, Percent_Identity=35.5491329479769, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI20129971, Length=235, Percent_Identity=27.6595744680851, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653477, Length=235, Percent_Identity=27.6595744680851, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653139, Length=200, Percent_Identity=26.5, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 38319; Mature: 38319

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKTSAAPVRAYVENSTGVLELNRPQALNSLTHEMVNIIDQALDQWWDDDAVHRVLVYS
CCCCCCCCHHHHEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEE
NSPKAFCAGGDVRAAREAIQAGRGGEADQFFVDEYDMNNNISEFPKPYISLIDGFVMGGG
CCCCEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHCCC
LGLSAHGSHRVISEKASAAMPEMAIGFTPDVGMAYMFQRMTNATGSTSHALAAFLVTTGW
CCCCCCCCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
RMSAADMIWSGLATDIVPSADHDLFRETVYAESLDEALERYSVDTVGDSELATLLPHIEK
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TFGFPTWAQINEALDTYPDREFVNTVTELLSTANPESLVAAVELMVASARSTTLREELDH
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVLGEYIRSRPNFAEGVRAVLVDKDRNADFTPATTGEVDVTPMRKLLKN
HHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNNKTSAAPVRAYVENSTGVLELNRPQALNSLTHEMVNIIDQALDQWWDDDAVHRVLVYS
CCCCCCCCHHHHEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEE
NSPKAFCAGGDVRAAREAIQAGRGGEADQFFVDEYDMNNNISEFPKPYISLIDGFVMGGG
CCCCEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHCCC
LGLSAHGSHRVISEKASAAMPEMAIGFTPDVGMAYMFQRMTNATGSTSHALAAFLVTTGW
CCCCCCCCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
RMSAADMIWSGLATDIVPSADHDLFRETVYAESLDEALERYSVDTVGDSELATLLPHIEK
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TFGFPTWAQINEALDTYPDREFVNTVTELLSTANPESLVAAVELMVASARSTTLREELDH
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVLGEYIRSRPNFAEGVRAVLVDKDRNADFTPATTGEVDVTPMRKLLKN
HHHHHHHHHCCCCHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA