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The map label for this gene is metG

Identifier: 38233469

GI number: 38233469

Start: 846041

End: 847873

Strand: Direct

Name: metG

Synonym: DIP0872

Alternate gene names: 38233469

Gene position: 846041-847873 (Clockwise)

Preceding gene: 38233468

Following gene: 38233470

Centisome position: 34.0

GC content: 53.9

Gene sequence:

>1833_bases
ATGAATAACCGTGTTCTCGTTTCTGTTGCTTGGCCTTATGCCAACGGCCCGCGCCATATCGGCCATGTGGCGGGCTTTGG
TGTTCCTTCTGATGTGTTCGCTCGCTACCAGCGAATGTCGGGCGCAGAGGTGCTCATGGTTTCAGGCACGGACGAGCACG
GCACCCCATTGTTGGTACAGGCAGATAAAGAAGGCGTATCAGTCAAGGAGCTTGCTGACCGCTACAACCGGCAGATCGTT
GAAGACCTCGCTGGTCTAGGGTTGTCCTACGATCTGTTCACCCGTACCACCACTCGTAATCACTACGCTGTCGTGCAGGA
CTTGTTCAAGGGCCTGTATGAAAACGGCTACATGATCAAAGAGACAACGATGGGTGCAGTGTCGCCATCAACAGGTAGGA
CGCTGCCGGACCGCTACATTGAAGGAACCTGTCCTATTTGTGGGGCTTCAGGAGCTCGTGGCGATCAATGCGATAACTGT
GGTAACCAGCTTGATCCAGCGGATTTGATCAATCCTGTATCGAAGATCAACGGTGAAACACCAAAGTTCATTGAAACGGA
GCATTTCCTTCTCGATCTTCCTGCTCTTGCGGATGCACTGGCTGCGTGGTTGAAGGATCGCAAAGACTGGCGCCCCAACG
TTTTGAAGTTTTCGCTGAATCTGTTGGAGGACCTGCGTCCTCGTGCTATGAGCCGTGACATCGACTGGGGTATTCCGATT
CCTGTCGAGGGCTGGCAGGACAACGGAGCAAAGAAACTCTACGTGTGGTTTGACGCAGTGGTGGGTTACCTATCTGCTTC
CATTGAGTGGGCTTATCGCACTGGCGATCCTGAGGCATGGAAGAAGTGGTGGAACGATCCGGAGTCCTCTGGTTACTACT
TCATGGGCAAGGACAACATCACGTTCCACTCCCAAATTTGGCCTGCTGAGCTCTTGGGCTACCAAGGCAAGGGAGCTAAA
GCTGGTTCGGTCCACAGCTTGGGTGAGCTGAACCTTCCCACAGAGGTTGTGTCTTCGGAGTTCCTGACGATGTCGGGCTC
GAAATTCTCCTCATCCAAGGGCATTGTCATTTACGTCAAGGACTTCCTCAAGGAATTCGGCGCTGATCCGCTGCGTTACT
TCATCGCCGTTGCGGGACCGGAAAACAACGACACGGACTTCACGTGGGATGAGTTTGTCCGCCGCGTGAACAACGAGCTT
GCTAACGGTTGGGGCAACCTAGTTAACCGTACGGTTTCGATGGCGTATAAGAACTTCGGTGAGGTTCCAACACCTGGTGA
GCTGACGGAATCAGATAAGAAGATTCTCGCTCAGGCCGAGGAAGCCTTTGGTGTTGTTGGTGAGGCGCTGGCACATTCTC
GCTTTAAGCAGGGCATTACGCACGCTATGCATATTGTTGGCGAAGCGAATGCTTACATTGCGGAGCAAGAGCCATGGAAG
CTTGCTAAGGATGAGTCGCAGCGTGAGCGGTTGGCTACAGTGCTGTGGACTGCTTTGCAGGTTGTGTCTGACTGCAACGT
GCTTCTTACCCCGTACTTGCCTCACATTGCCCAGCAGGTGCATGAGACGCTGGGACGCGATGGCGTGTGGGCTGCTAAAC
CACAGATCGTTGAGGTTACTGACGACATGCCCGTTGAACCTATTGGCGTGGGTATTCCAGAGGCTGGTCAGACCTACCCT
GTGATTATGGGTGACTACGCAGCACAGCAGGCACGGTGGGCTCGTATCGACGTTCAACCTGGTACTGCACTATCGAAGCC
GAAGCCGCTCATTGCCAAGTTGGATCCAGAGCTTGGAGAAACCGGCCCTGAGTGGGCTCCAGTTAATCCGTAG

Upstream 100 bases:

>100_bases
GTGGTGAAGTTGCTGCCCACTATGGGGTGAGCAAGAAACAACTGTATGATGCCGTCCTCGAATCACGTGCTGAGTAGGAA
ATAAATTAAGGTATATAACC

Downstream 100 bases:

>100_bases
TTCTTTTCTATACTGGCTAGCATGTCAAAAAAGAAGCCTCGTCCGATTCCTGTTCCAGCCGATACCATTCCAGGGCTTAT
CGACGCCCACACACACATTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MNNRVLVSVAWPYANGPRHIGHVAGFGVPSDVFARYQRMSGAEVLMVSGTDEHGTPLLVQADKEGVSVKELADRYNRQIV
EDLAGLGLSYDLFTRTTTRNHYAVVQDLFKGLYENGYMIKETTMGAVSPSTGRTLPDRYIEGTCPICGASGARGDQCDNC
GNQLDPADLINPVSKINGETPKFIETEHFLLDLPALADALAAWLKDRKDWRPNVLKFSLNLLEDLRPRAMSRDIDWGIPI
PVEGWQDNGAKKLYVWFDAVVGYLSASIEWAYRTGDPEAWKKWWNDPESSGYYFMGKDNITFHSQIWPAELLGYQGKGAK
AGSVHSLGELNLPTEVVSSEFLTMSGSKFSSSKGIVIYVKDFLKEFGADPLRYFIAVAGPENNDTDFTWDEFVRRVNNEL
ANGWGNLVNRTVSMAYKNFGEVPTPGELTESDKKILAQAEEAFGVVGEALAHSRFKQGITHAMHIVGEANAYIAEQEPWK
LAKDESQRERLATVLWTALQVVSDCNVLLTPYLPHIAQQVHETLGRDGVWAAKPQIVEVTDDMPVEPIGVGIPEAGQTYP
VIMGDYAAQQARWARIDVQPGTALSKPKPLIAKLDPELGETGPEWAPVNP

Sequences:

>Translated_610_residues
MNNRVLVSVAWPYANGPRHIGHVAGFGVPSDVFARYQRMSGAEVLMVSGTDEHGTPLLVQADKEGVSVKELADRYNRQIV
EDLAGLGLSYDLFTRTTTRNHYAVVQDLFKGLYENGYMIKETTMGAVSPSTGRTLPDRYIEGTCPICGASGARGDQCDNC
GNQLDPADLINPVSKINGETPKFIETEHFLLDLPALADALAAWLKDRKDWRPNVLKFSLNLLEDLRPRAMSRDIDWGIPI
PVEGWQDNGAKKLYVWFDAVVGYLSASIEWAYRTGDPEAWKKWWNDPESSGYYFMGKDNITFHSQIWPAELLGYQGKGAK
AGSVHSLGELNLPTEVVSSEFLTMSGSKFSSSKGIVIYVKDFLKEFGADPLRYFIAVAGPENNDTDFTWDEFVRRVNNEL
ANGWGNLVNRTVSMAYKNFGEVPTPGELTESDKKILAQAEEAFGVVGEALAHSRFKQGITHAMHIVGEANAYIAEQEPWK
LAKDESQRERLATVLWTALQVVSDCNVLLTPYLPHIAQQVHETLGRDGVWAAKPQIVEVTDDMPVEPIGVGIPEAGQTYP
VIMGDYAAQQARWARIDVQPGTALSKPKPLIAKLDPELGETGPEWAPVNP
>Mature_610_residues
MNNRVLVSVAWPYANGPRHIGHVAGFGVPSDVFARYQRMSGAEVLMVSGTDEHGTPLLVQADKEGVSVKELADRYNRQIV
EDLAGLGLSYDLFTRTTTRNHYAVVQDLFKGLYENGYMIKETTMGAVSPSTGRTLPDRYIEGTCPICGASGARGDQCDNC
GNQLDPADLINPVSKINGETPKFIETEHFLLDLPALADALAAWLKDRKDWRPNVLKFSLNLLEDLRPRAMSRDIDWGIPI
PVEGWQDNGAKKLYVWFDAVVGYLSASIEWAYRTGDPEAWKKWWNDPESSGYYFMGKDNITFHSQIWPAELLGYQGKGAK
AGSVHSLGELNLPTEVVSSEFLTMSGSKFSSSKGIVIYVKDFLKEFGADPLRYFIAVAGPENNDTDFTWDEFVRRVNNEL
ANGWGNLVNRTVSMAYKNFGEVPTPGELTESDKKILAQAEEAFGVVGEALAHSRFKQGITHAMHIVGEANAYIAEQEPWK
LAKDESQRERLATVLWTALQVVSDCNVLLTPYLPHIAQQVHETLGRDGVWAAKPQIVEVTDDMPVEPIGVGIPEAGQTYP
VIMGDYAAQQARWARIDVQPGTALSKPKPLIAKLDPELGETGPEWAPVNP

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=528, Percent_Identity=33.5227272727273, Blast_Score=334, Evalue=2e-91,
Organism=Homo sapiens, GI24308436, Length=583, Percent_Identity=27.2727272727273, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1788432, Length=530, Percent_Identity=30.7547169811321, Blast_Score=280, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17540794, Length=530, Percent_Identity=31.6981132075472, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI212645096, Length=527, Percent_Identity=27.5142314990512, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71992781, Length=363, Percent_Identity=27.5482093663912, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321704, Length=532, Percent_Identity=32.8947368421053, Blast_Score=306, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=24.0437158469945, Blast_Score=123, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24655311, Length=532, Percent_Identity=33.4586466165414, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI21358483, Length=550, Percent_Identity=25.4545454545455, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CORDI (Q6NIA5)

Other databases:

- EMBL:   BX248356
- RefSeq:   NP_939236.1
- ProteinModelPortal:   Q6NIA5
- GeneID:   2648486
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0872
- NMPDR:   fig|257309.1.peg.827
- HOGENOM:   HBG721667
- OMA:   CGNQLDP
- PhylomeDB:   Q6NIA5
- ProtClustDB:   PRK12268
- BioCyc:   CDIP257309:DIP0872-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 67462; Mature: 67462

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS00639 THIOL_PROTEASE_HIS

Important sites: BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRVLVSVAWPYANGPRHIGHVAGFGVPSDVFARYQRMSGAEVLMVSGTDEHGTPLLVQ
CCCEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
ADKEGVSVKELADRYNRQIVEDLAGLGLSYDLFTRTTTRNHYAVVQDLFKGLYENGYMIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHEECCCCCHHHHHHHHHHHHHHCCEEEE
ETTMGAVSPSTGRTLPDRYIEGTCPICGASGARGDQCDNCGNQLDPADLINPVSKINGET
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PKFIETEHFLLDLPALADALAAWLKDRKDWRPNVLKFSLNLLEDLRPRAMSRDIDWGIPI
CCEECCCHHEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCC
PVEGWQDNGAKKLYVWFDAVVGYLSASIEWAYRTGDPEAWKKWWNDPESSGYYFMGKDNI
CCCCCCCCCCEEEEEHHHHHHHHHHHCEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCE
TFHSQIWPAELLGYQGKGAKAGSVHSLGELNLPTEVVSSEFLTMSGSKFSSSKGIVIYVK
EEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEEEHH
DFLKEFGADPLRYFIAVAGPENNDTDFTWDEFVRRVNNELANGWGNLVNRTVSMAYKNFG
HHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVPTPGELTESDKKILAQAEEAFGVVGEALAHSRFKQGITHAMHIVGEANAYIAEQEPWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LAKDESQRERLATVLWTALQVVSDCNVLLTPYLPHIAQQVHETLGRDGVWAAKPQIVEVT
CCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEECCCEEEEEC
DDMPVEPIGVGIPEAGQTYPVIMGDYAAQQARWARIDVQPGTALSKPKPLIAKLDPELGE
CCCCCCCCCCCCCCCCCCCCEEECCHHHHCCCEEEEECCCCCCCCCCCCCCEECCCHHCC
TGPEWAPVNP
CCCCCCCCCC
>Mature Secondary Structure
MNNRVLVSVAWPYANGPRHIGHVAGFGVPSDVFARYQRMSGAEVLMVSGTDEHGTPLLVQ
CCCEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
ADKEGVSVKELADRYNRQIVEDLAGLGLSYDLFTRTTTRNHYAVVQDLFKGLYENGYMIK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHEECCCCCHHHHHHHHHHHHHHCCEEEE
ETTMGAVSPSTGRTLPDRYIEGTCPICGASGARGDQCDNCGNQLDPADLINPVSKINGET
ECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PKFIETEHFLLDLPALADALAAWLKDRKDWRPNVLKFSLNLLEDLRPRAMSRDIDWGIPI
CCEECCCHHEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCCC
PVEGWQDNGAKKLYVWFDAVVGYLSASIEWAYRTGDPEAWKKWWNDPESSGYYFMGKDNI
CCCCCCCCCCEEEEEHHHHHHHHHHHCEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCE
TFHSQIWPAELLGYQGKGAKAGSVHSLGELNLPTEVVSSEFLTMSGSKFSSSKGIVIYVK
EEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEEEHH
DFLKEFGADPLRYFIAVAGPENNDTDFTWDEFVRRVNNELANGWGNLVNRTVSMAYKNFG
HHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVPTPGELTESDKKILAQAEEAFGVVGEALAHSRFKQGITHAMHIVGEANAYIAEQEPWK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
LAKDESQRERLATVLWTALQVVSDCNVLLTPYLPHIAQQVHETLGRDGVWAAKPQIVEVT
CCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCCEEECCCEEEEEC
DDMPVEPIGVGIPEAGQTYPVIMGDYAAQQARWARIDVQPGTALSKPKPLIAKLDPELGE
CCCCCCCCCCCCCCCCCCCCEEECCHHHHCCCEEEEECCCCCCCCCCCCCCEECCCHHCC
TGPEWAPVNP
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910