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The map label for this gene is citE [H]

Identifier: 38233442

GI number: 38233442

Start: 822822

End: 823655

Strand: Direct

Name: citE [H]

Synonym: DIP0845

Alternate gene names: 38233442

Gene position: 822822-823655 (Clockwise)

Preceding gene: 38233441

Following gene: 38233449

Centisome position: 33.06

GC content: 54.68

Gene sequence:

>834_bases
ATGACGAATCGAGAGCTGATATTACGGCCTGCGGTCCTGTTTGCCCCAGCGAATAGGCCTGAGATTATTCCCAAAGCTGC
CGCCCGTGCAGATATGGTCATCCTAGATTTAGAGGATGGGGCGGGCTCTATTGATCGCAACCAAGCGTACGCCAATATTC
GCAACGCGGGTTTGGATCCGACAACAACATTTGTTCGGATTGTAGGACCAGAAGATCCTCACCATGCTGCGGATCTCGCG
TTTGTTAAGACCACAGAGTATTACAACGTGATCGTGCCGAAGATCGGCGCTGCTCTCCCCGAAGGCCTTGATGGCCTCAA
TGTTGTACCGATTATTGAAACCCCATTGGCTGTTATCAATATAGCCTCGATTGCTGCGGATCCCCACGTGGTCGGGTTGT
ACTGGGGTGCTGATGATCTTACGATCGCGCTCGGCGGGTTGTATTCGCGTCGGCGTATCGACGAGCCCCAGCCTTCCTCC
TACAGGGCACCCATCGAGTATGCACGCGTGCAGACGCTGCTGCACGCTGCTGCGAATGGTAAATGGGCATTGGATGCGGT
CTATCAGGATTTCAAAGACCTAGATGGCCTCTATACAGAGGCTTTGGACTCTGCACGCATGGGATTTGCTGCGTATCCAT
GCATTCATCCGAACCAAGTTGATGCGGTCCGGCGGGCTTTCGCACCTAGCGCAGAGCAGTTGGAGTGGGCGCAGCGTATC
GCTGAGGAAACGAAAAAACACCCCGGAGCTTTTCAGCTCGACGGGGAGATGGTAGATACGCCGCTCATTGCGTTGGCACA
TCGGTTGCTGCAGCGTCACGCTGCAGTGGATTAG

Upstream 100 bases:

>100_bases
GCGACCAAGCTCGCTAGCTTTAATGGAAATGTTCCGTCAGCGGAATGTGTGTGATCTTTTGGGCATCATTGGCTAACAGT
TCGTCGCTAGGATTGTCGTT

Downstream 100 bases:

>100_bases
TCGTTTAGGGTGCGAATTAGGTTCAGTGTCCATTCCACGCGGTCATCGGGCAGTTCCGCACCTACGACGGCGAGTGCGGC
GTCGGCAAGCATGATTGCTA

Product: putative citrate lyase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTNRELILRPAVLFAPANRPEIIPKAAARADMVILDLEDGAGSIDRNQAYANIRNAGLDPTTTFVRIVGPEDPHHAADLA
FVKTTEYYNVIVPKIGAALPEGLDGLNVVPIIETPLAVINIASIAADPHVVGLYWGADDLTIALGGLYSRRRIDEPQPSS
YRAPIEYARVQTLLHAAANGKWALDAVYQDFKDLDGLYTEALDSARMGFAAYPCIHPNQVDAVRRAFAPSAEQLEWAQRI
AEETKKHPGAFQLDGEMVDTPLIALAHRLLQRHAAVD

Sequences:

>Translated_277_residues
MTNRELILRPAVLFAPANRPEIIPKAAARADMVILDLEDGAGSIDRNQAYANIRNAGLDPTTTFVRIVGPEDPHHAADLA
FVKTTEYYNVIVPKIGAALPEGLDGLNVVPIIETPLAVINIASIAADPHVVGLYWGADDLTIALGGLYSRRRIDEPQPSS
YRAPIEYARVQTLLHAAANGKWALDAVYQDFKDLDGLYTEALDSARMGFAAYPCIHPNQVDAVRRAFAPSAEQLEWAQRI
AEETKKHPGAFQLDGEMVDTPLIALAHRLLQRHAAVD
>Mature_276_residues
TNRELILRPAVLFAPANRPEIIPKAAARADMVILDLEDGAGSIDRNQAYANIRNAGLDPTTTFVRIVGPEDPHHAADLAF
VKTTEYYNVIVPKIGAALPEGLDGLNVVPIIETPLAVINIASIAADPHVVGLYWGADDLTIALGGLYSRRRIDEPQPSSY
RAPIEYARVQTLLHAAANGKWALDAVYQDFKDLDGLYTEALDSARMGFAAYPCIHPNQVDAVRRAFAPSAEQLEWAQRIA
EETKKHPGAFQLDGEMVDTPLIALAHRLLQRHAAVD

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=303, Percent_Identity=28.3828382838284, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI87081764, Length=298, Percent_Identity=25.1677852348993, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557458, Length=159, Percent_Identity=30.188679245283, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30229; Mature: 30097

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRELILRPAVLFAPANRPEIIPKAAARADMVILDLEDGAGSIDRNQAYANIRNAGLDP
CCCCCEEEECEEEEECCCCCCCCCHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCC
TTTFVRIVGPEDPHHAADLAFVKTTEYYNVIVPKIGAALPEGLDGLNVVPIIETPLAVIN
CCEEEEEECCCCCCCHHCEEEEEECCHHEEEHHHHHHHCCCCCCCCEEEEEECCCHHHHH
IASIAADPHVVGLYWGADDLTIALGGLYSRRRIDEPQPSSYRAPIEYARVQTLLHAAANG
HHHHCCCCCEEEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KWALDAVYQDFKDLDGLYTEALDSARMGFAAYPCIHPNQVDAVRRAFAPSAEQLEWAQRI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AEETKKHPGAFQLDGEMVDTPLIALAHRLLQRHAAVD
HHHHHCCCCCEEECCCHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNRELILRPAVLFAPANRPEIIPKAAARADMVILDLEDGAGSIDRNQAYANIRNAGLDP
CCCCEEEECEEEEECCCCCCCCCHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCC
TTTFVRIVGPEDPHHAADLAFVKTTEYYNVIVPKIGAALPEGLDGLNVVPIIETPLAVIN
CCEEEEEECCCCCCCHHCEEEEEECCHHEEEHHHHHHHCCCCCCCCEEEEEECCCHHHHH
IASIAADPHVVGLYWGADDLTIALGGLYSRRRIDEPQPSSYRAPIEYARVQTLLHAAANG
HHHHCCCCCEEEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
KWALDAVYQDFKDLDGLYTEALDSARMGFAAYPCIHPNQVDAVRRAFAPSAEQLEWAQRI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AEETKKHPGAFQLDGEMVDTPLIALAHRLLQRHAAVD
HHHHHCCCCCEEECCCHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]