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The map label for this gene is manB [H]

Identifier: 38233294

GI number: 38233294

Start: 661191

End: 662567

Strand: Direct

Name: manB [H]

Synonym: DIP0687

Alternate gene names: 38233294

Gene position: 661191-662567 (Clockwise)

Preceding gene: 38233293

Following gene: 38233295

Centisome position: 26.57

GC content: 55.92

Gene sequence:

>1377_bases
ATGCGTACCCGTGAATCGGTAACGAAGGTGATTAAGGCATATGACGTCCGCGGCGTCGTCGGCGTAGACATCGACAGCGA
CTTCATTGCAGAGGTTGGTGCAGCGTTTGGTGCGCTGATGCGCGGTGAAGGCGCAGCTAGGGTTGTCATCGGTCATGACA
TGCGGCCATCTTCGCCAGAGCTTGCCGACGCCTTCGCTCGCGGTGTGGTATCTCAGGGCGTCAATGTCATCCAACTGGGG
CTTACCTCTACCGACCAGCTGTACTGCGCCTCGGGCGAGCTGGATTGCCCTGGCGCGATGTTCACGGCGAGCCACAACCC
TGCCGAGTACAACGGCATCAAGATGTGCCGCAGTGGCGCCCGTCCCGTGGGACAAGAAACCGGGTTGGCGCAGATCATCG
ACATGCTTGTCGACGGAATCCCAGCATTTGATGGTCCACAAGGCGAAATCTCACATCGAGACACCTTGGCTGATTACGCA
GCATTCCTGCGCAACTTGGTCGATTTGTCCAACATTCGTCCGCTGGTTGTGGCTGTTGACGCCGCCAATGGCATGGGTGG
CCACACCGTTCCTGAAGTATTTGCGGGTCTGCCACTTGAGATTAAACCGCTCTACTTTGAACTCGACGGCACGTTCCCTA
ACCATGAGGCCAACCCACTAGACCCGAAGAACTTGGTGGATCTGCAAAAGTTCACCCCTGAGGTCGGTGCTGACATCGGT
CTAGCATTCGACGGAGATGCGGATCGCTGCTTTGTTGTCGATGAGCTTGGCCAGCCGGTCAGCCCTTCGGCCATTTGTGC
CATCGTCGCTGAGCGCTATTTGGCACAACACCCAAGCGCAACAGTAATCCACAACCTGATTACATCGAAGTCTGTGCCGG
AAATCATCGCAGAAAACGGTGGTAGCGCCGTGCGTACTCGCGTGGGACACTCCTTTATCAAAGCCCAAATGGCTGAGCAC
TCCGCAGTATTCGGTGGCGAGCATTCCGCGCACTACTACTTCTCGGAGTTCTTCAACGCTGACTCCGGAATCCTTGCTGC
CATGCACGTTTTGGCGGCTCTAGGTGGCCAAGATAAGCCGCTTTCGGAGCTCATGCATCAGTACAACCGTTACCAAGCAT
CCGGAGAGATCAACTCTAGGCTGGCAAGCTCCGAAGCACAGGCAGAGCGTACCCAAGCGGTACTAGATGCCTTTGCCGAT
CGTATCGAAAGCGTTGATCGCCTCGACGGTGTCACTGTAGAACTCAAGGACACCAAGGCTTGGTTTAATGTTCGTGCATC
GAACACCGAACCCCTGCTACGGCTCAATGTTGAGGCTGAAACCGACCAAGAAGTGGCAGCGCTCGTCGAAGAGATTCTCG
CTATCATTCGCACATAA

Upstream 100 bases:

>100_bases
TAGGGCATAAAGCTGGACTGCGGGCGGACTCGTATCCGTCCTAGGGGGTAGAATCCCTGTGAATACTTTTGTGCATCCCT
GAAAGGTGGGATTTTCTTTC

Downstream 100 bases:

>100_bases
TCTTTAGGAGAAGAAGCATCACAGTGGCGAAGCCTATAATGGCCACTGATGCATACTTCTTCGTGGACTACACCAACGGC
TAGTGGGGCATATGACCCAC

Product: phosphomannomutase/phosphoglucomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRTRESVTKVIKAYDVRGVVGVDIDSDFIAEVGAAFGALMRGEGAARVVIGHDMRPSSPELADAFARGVVSQGVNVIQLG
LTSTDQLYCASGELDCPGAMFTASHNPAEYNGIKMCRSGARPVGQETGLAQIIDMLVDGIPAFDGPQGEISHRDTLADYA
AFLRNLVDLSNIRPLVVAVDAANGMGGHTVPEVFAGLPLEIKPLYFELDGTFPNHEANPLDPKNLVDLQKFTPEVGADIG
LAFDGDADRCFVVDELGQPVSPSAICAIVAERYLAQHPSATVIHNLITSKSVPEIIAENGGSAVRTRVGHSFIKAQMAEH
SAVFGGEHSAHYYFSEFFNADSGILAAMHVLAALGGQDKPLSELMHQYNRYQASGEINSRLASSEAQAERTQAVLDAFAD
RIESVDRLDGVTVELKDTKAWFNVRASNTEPLLRLNVEAETDQEVAALVEEILAIIRT

Sequences:

>Translated_458_residues
MRTRESVTKVIKAYDVRGVVGVDIDSDFIAEVGAAFGALMRGEGAARVVIGHDMRPSSPELADAFARGVVSQGVNVIQLG
LTSTDQLYCASGELDCPGAMFTASHNPAEYNGIKMCRSGARPVGQETGLAQIIDMLVDGIPAFDGPQGEISHRDTLADYA
AFLRNLVDLSNIRPLVVAVDAANGMGGHTVPEVFAGLPLEIKPLYFELDGTFPNHEANPLDPKNLVDLQKFTPEVGADIG
LAFDGDADRCFVVDELGQPVSPSAICAIVAERYLAQHPSATVIHNLITSKSVPEIIAENGGSAVRTRVGHSFIKAQMAEH
SAVFGGEHSAHYYFSEFFNADSGILAAMHVLAALGGQDKPLSELMHQYNRYQASGEINSRLASSEAQAERTQAVLDAFAD
RIESVDRLDGVTVELKDTKAWFNVRASNTEPLLRLNVEAETDQEVAALVEEILAIIRT
>Mature_458_residues
MRTRESVTKVIKAYDVRGVVGVDIDSDFIAEVGAAFGALMRGEGAARVVIGHDMRPSSPELADAFARGVVSQGVNVIQLG
LTSTDQLYCASGELDCPGAMFTASHNPAEYNGIKMCRSGARPVGQETGLAQIIDMLVDGIPAFDGPQGEISHRDTLADYA
AFLRNLVDLSNIRPLVVAVDAANGMGGHTVPEVFAGLPLEIKPLYFELDGTFPNHEANPLDPKNLVDLQKFTPEVGADIG
LAFDGDADRCFVVDELGQPVSPSAICAIVAERYLAQHPSATVIHNLITSKSVPEIIAENGGSAVRTRVGHSFIKAQMAEH
SAVFGGEHSAHYYFSEFFNADSGILAAMHVLAALGGQDKPLSELMHQYNRYQASGEINSRLASSEAQAERTQAVLDAFAD
RIESVDRLDGVTVELKDTKAWFNVRASNTEPLLRLNVEAETDQEVAALVEEILAIIRT

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=444, Percent_Identity=36.4864864864865, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1789566, Length=449, Percent_Identity=26.0579064587973, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 49103; Mature: 49103

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRESVTKVIKAYDVRGVVGVDIDSDFIAEVGAAFGALMRGEGAARVVIGHDMRPSSPE
CCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
LADAFARGVVSQGVNVIQLGLTSTDQLYCASGELDCPGAMFTASHNPAEYNGIKMCRSGA
HHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCHHHHHHHCCC
RPVGQETGLAQIIDMLVDGIPAFDGPQGEISHRDTLADYAAFLRNLVDLSNIRPLVVAVD
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
AANGMGGHTVPEVFAGLPLEIKPLYFELDGTFPNHEANPLDPKNLVDLQKFTPEVGADIG
CCCCCCCCCHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHCCCEE
LAFDGDADRCFVVDELGQPVSPSAICAIVAERYLAQHPSATVIHNLITSKSVPEIIAENG
EEECCCCCEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCC
GSAVRTRVGHSFIKAQMAEHSAVFGGEHSAHYYFSEFFNADSGILAAMHVLAALGGQDKP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
LSELMHQYNRYQASGEINSRLASSEAQAERTQAVLDAFADRIESVDRLDGVTVELKDTKA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
WFNVRASNTEPLLRLNVEAETDQEVAALVEEILAIIRT
EEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTRESVTKVIKAYDVRGVVGVDIDSDFIAEVGAAFGALMRGEGAARVVIGHDMRPSSPE
CCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCH
LADAFARGVVSQGVNVIQLGLTSTDQLYCASGELDCPGAMFTASHNPAEYNGIKMCRSGA
HHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCHHHHHHHCCC
RPVGQETGLAQIIDMLVDGIPAFDGPQGEISHRDTLADYAAFLRNLVDLSNIRPLVVAVD
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
AANGMGGHTVPEVFAGLPLEIKPLYFELDGTFPNHEANPLDPKNLVDLQKFTPEVGADIG
CCCCCCCCCHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHCCCEE
LAFDGDADRCFVVDELGQPVSPSAICAIVAERYLAQHPSATVIHNLITSKSVPEIIAENG
EEECCCCCEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCC
GSAVRTRVGHSFIKAQMAEHSAVFGGEHSAHYYFSEFFNADSGILAAMHVLAALGGQDKP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
LSELMHQYNRYQASGEINSRLASSEAQAERTQAVLDAFADRIESVDRLDGVTVELKDTKA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCE
WFNVRASNTEPLLRLNVEAETDQEVAALVEEILAIIRT
EEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]