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The map label for this gene is dacD [H]

Identifier: 38233244

GI number: 38233244

Start: 608070

End: 609302

Strand: Reverse

Name: dacD [H]

Synonym: DIP0637

Alternate gene names: 38233244

Gene position: 609302-608070 (Counterclockwise)

Preceding gene: 38233246

Following gene: 38233238

Centisome position: 24.48

GC content: 55.72

Gene sequence:

>1233_bases
ATGAAACGTGTGCCAAAAACCCTCATCGCGCTGACCGCCATCACTGCAACACTCTCACCCACCGCACTTAGCGCAACCAT
GGCACCAGTATTAGCTGATCCCGCACCGCGTTCCTCCGCGCCGAACACCGACAAATGCGACAACGCTACCACCCCACCAT
CGGCCGAGACGACCTCCGAAAAACTCGCACCAGGCCGTACTTCAATCGCACCTCTTCCCACCGTCGACAACGAATTTGGC
AGCTGTGGGATAACAAAAGCAACAGGATTCGACGTCCCCACCAATACCGCTTCGGCATGGATGGTTTTTGACCTCGATAC
CGGTGCTGTCATCGCCACCAAAGACCCCCACGGACGCTACCGTCCTGCATCCGTCATCAAAGCCCTCATCGCGCTCGTCG
CAATCGACGAACTCGACCTTTCGAAAAAAGTCACCATCACCAATGACGACGTGAACCAAGAAGGCTCCGCTGTCGGATTT
GTGGAAGGAATCGACTATTCCGTCCAACAACTACTCGACGGACTACTCCTCGGCAGCGGAAACGATGCCGCCAGTGCACT
CGCACGCGCCCTCGGCGGCGAAGACACCACCATCAAAAAGATCAACGATCTTGCGCAGGAATTGGGAACCGTCGACACGT
ACGCCGCCTCGTACACTGGCCTGGATGCTCAAGGCATGTCCACTTCTGTGCGCGACCTTGCTGTGATCTATCAAGCAGCA
TGGCAAAACCCCGTCTTCGCAGACACCGTGAAAAAGGAATACTCAGAACTCCCCGCCGATAAAACCGGCACAACATTCCA
AGTCTGGAATGACAACGGCATCCTCATGAACACCGAACACGGCATCGGCGGTAAAACCGGCTTTACTGACGACGCCCACC
ACACCTTCGTCGGCGCCAAAAACATCAACGGTCGCCGCATCGCCGCCGTCATCCTCGACACCACCATCGACCTCGGACGT
CCATGGGAACAAGCCAACAAGCTTATCGACGCCGGCTACAAAGTCGCCGAAAACCAAGCAGTAGGGTCGATTCTTCGGAA
GGAATCGTCGATTCCTTCCGAAGAATCGCAACCAAAAGAACTCTCGCGCCCATCGTTTACTAATCCTTCTGATAACCGCG
ACACGACACCTCGTTTTGTGATTCTGGGGGCGTTGGTGGCGCTGGTGGTGCTCGGGGCACTGGCATGGATGTTCGGTAAA
CGCGAAGATCGTGAAGATCGTGTAGATCGTTAA

Upstream 100 bases:

>100_bases
TAGTGCGTGAATTAGCTCATGCGAAATTACTTTAAATTCTGAACGGAAAATAGGGGTGTCATACATGAACTACACCTTAA
GCCACGGCACCATCATAAGT

Downstream 100 bases:

>100_bases
AACAGGAAACCTCTGGGTACGCAGACGATGTGTATCATCGTCTGCGTACCCAGAGGGTTGTTTTTATTTTGTTGAATCAG
GCGGATTCTAGGAGTCGTTG

Product: putative peptidoglycan synthesis-like protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKRVPKTLIALTAITATLSPTALSATMAPVLADPAPRSSAPNTDKCDNATTPPSAETTSEKLAPGRTSIAPLPTVDNEFG
SCGITKATGFDVPTNTASAWMVFDLDTGAVIATKDPHGRYRPASVIKALIALVAIDELDLSKKVTITNDDVNQEGSAVGF
VEGIDYSVQQLLDGLLLGSGNDAASALARALGGEDTTIKKINDLAQELGTVDTYAASYTGLDAQGMSTSVRDLAVIYQAA
WQNPVFADTVKKEYSELPADKTGTTFQVWNDNGILMNTEHGIGGKTGFTDDAHHTFVGAKNINGRRIAAVILDTTIDLGR
PWEQANKLIDAGYKVAENQAVGSILRKESSIPSEESQPKELSRPSFTNPSDNRDTTPRFVILGALVALVVLGALAWMFGK
REDREDRVDR

Sequences:

>Translated_410_residues
MKRVPKTLIALTAITATLSPTALSATMAPVLADPAPRSSAPNTDKCDNATTPPSAETTSEKLAPGRTSIAPLPTVDNEFG
SCGITKATGFDVPTNTASAWMVFDLDTGAVIATKDPHGRYRPASVIKALIALVAIDELDLSKKVTITNDDVNQEGSAVGF
VEGIDYSVQQLLDGLLLGSGNDAASALARALGGEDTTIKKINDLAQELGTVDTYAASYTGLDAQGMSTSVRDLAVIYQAA
WQNPVFADTVKKEYSELPADKTGTTFQVWNDNGILMNTEHGIGGKTGFTDDAHHTFVGAKNINGRRIAAVILDTTIDLGR
PWEQANKLIDAGYKVAENQAVGSILRKESSIPSEESQPKELSRPSFTNPSDNRDTTPRFVILGALVALVVLGALAWMFGK
REDREDRVDR
>Mature_410_residues
MKRVPKTLIALTAITATLSPTALSATMAPVLADPAPRSSAPNTDKCDNATTPPSAETTSEKLAPGRTSIAPLPTVDNEFG
SCGITKATGFDVPTNTASAWMVFDLDTGAVIATKDPHGRYRPASVIKALIALVAIDELDLSKKVTITNDDVNQEGSAVGF
VEGIDYSVQQLLDGLLLGSGNDAASALARALGGEDTTIKKINDLAQELGTVDTYAASYTGLDAQGMSTSVRDLAVIYQAA
WQNPVFADTVKKEYSELPADKTGTTFQVWNDNGILMNTEHGIGGKTGFTDDAHHTFVGAKNINGRRIAAVILDTTIDLGR
PWEQANKLIDAGYKVAENQAVGSILRKESSIPSEESQPKELSRPSFTNPSDNRDTTPRFVILGALVALVVLGALAWMFGK
REDREDRVDR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=276, Percent_Identity=31.5217391304348, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786851, Length=263, Percent_Identity=30.7984790874525, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787062, Length=261, Percent_Identity=27.9693486590038, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082054, Length=221, Percent_Identity=30.316742081448, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43592; Mature: 43592

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVPKTLIALTAITATLSPTALSATMAPVLADPAPRSSAPNTDKCDNATTPPSAETTSE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KLAPGRTSIAPLPTVDNEFGSCGITKATGFDVPTNTASAWMVFDLDTGAVIATKDPHGRY
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCC
RPASVIKALIALVAIDELDLSKKVTITNDDVNQEGSAVGFVEGIDYSVQQLLDGLLLGSG
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHEECCC
NDAASALARALGGEDTTIKKINDLAQELGTVDTYAASYTGLDAQGMSTSVRDLAVIYQAA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
WQNPVFADTVKKEYSELPADKTGTTFQVWNDNGILMNTEHGIGGKTGFTDDAHHTFVGAK
HCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEEEEECC
NINGRRIAAVILDTTIDLGRPWEQANKLIDAGYKVAENQAVGSILRKESSIPSEESQPKE
CCCCCEEEEEEEECHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
LSRPSFTNPSDNRDTTPRFVILGALVALVVLGALAWMFGKREDREDRVDR
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MKRVPKTLIALTAITATLSPTALSATMAPVLADPAPRSSAPNTDKCDNATTPPSAETTSE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KLAPGRTSIAPLPTVDNEFGSCGITKATGFDVPTNTASAWMVFDLDTGAVIATKDPHGRY
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCC
RPASVIKALIALVAIDELDLSKKVTITNDDVNQEGSAVGFVEGIDYSVQQLLDGLLLGSG
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHEECCC
NDAASALARALGGEDTTIKKINDLAQELGTVDTYAASYTGLDAQGMSTSVRDLAVIYQAA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
WQNPVFADTVKKEYSELPADKTGTTFQVWNDNGILMNTEHGIGGKTGFTDDAHHTFVGAK
HCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCEEEEECC
NINGRRIAAVILDTTIDLGRPWEQANKLIDAGYKVAENQAVGSILRKESSIPSEESQPKE
CCCCCEEEEEEEECHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
LSRPSFTNPSDNRDTTPRFVILGALVALVVLGALAWMFGKREDREDRVDR
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]