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The map label for this gene is exoA [H]

Identifier: 38233240

GI number: 38233240

Start: 604219

End: 605124

Strand: Direct

Name: exoA [H]

Synonym: DIP0633

Alternate gene names: 38233240

Gene position: 604219-605124 (Clockwise)

Preceding gene: 38233239

Following gene: 38233241

Centisome position: 24.28

GC content: 53.53

Gene sequence:

>906_bases
ATGCAAATTACAACAGTTAACGTCAATGGAATTCGTGCTGCCGTCAAGCAGCGTAGCGACGCCAACCGCGGAATGCTTCC
GTGGCTAGAGCAGTGTGGCTCCGATGTCGTGTTGCTGCAAGAGGTTCGTGCTTCGGAAAAAGATTCTCTTACTGCTTTGG
CACCTGCCCTCGATGCCGGATGGCACTATGTTGGCGCTCCTGCTGCTGCCAAAGGCCGCGCCGGGGTGGGTATTTTAAGT
CGCCAGCCACTGCGTGATGTTCAGGTGGGGTTGGAAGGATTCGTCGATTCTGGCCGGTACATCCAAGCAACACTGTCTGA
TCCTGATACAGGTCATGACGTGGTGGTGGCGTCGCTCTACTTGCCTTCTGGTTCTGCCGACACTGATAAGCAAGACGAGA
AATACCGTTTCCTTGATGTGTTCGGTCCGTTTCTTGAGCAGGCAGCGCAGGATAATCCGCACATGGTGGTCGGTGGCGAT
TGGAACATTTGTCACCGCATGCAAGATCTGAAGAATTGGAAAACAAACCGAAAGAAGTCTGGATTCCTTCCAGACGAACG
GGCTTTTATGGATTCGGTATTTGGTGTTTTCCCTGATTCTGAAAGCCAGGTGTCCGATGCTGGCCAATGGGCAGGTGCGG
TGGACTACACCTCGCAGCAGCGTCGTACTCCGGCGAACGACCCGAAATGGTTTGACGTGGCGCGTCGATTAGCTCCCGAG
GATGCTGCTTATACTTGGTGGACTTATCGTGGCCAGGCGTTTGATACGAATGCTGGTTGGCGTATTGATTATCAAGCAGC
AACTTCTGCGATGCTGGAGCGTGCGACTCGGTGCTGGGTCGATAAGGCAGCAGCATATGATTTGCGCTGGTCGGATCACT
CACCTTTGACGGTGGTGTATTCCTAG

Upstream 100 bases:

>100_bases
GCAGGCGCTGCTTTAGGTTGTGCCGCCATCATCATTTGGATCCCAGCGCATATCACGCGCAGCAAACAAGAACGCAAACA
GCACAGCTAGGATGGCCAGC

Downstream 100 bases:

>100_bases
TCTTCGAGACGCCATTACGCACTGACGACGCCCTGTTTACTCTAAGGCGTGGGGCTAAGGTCAGACACCTCGTTTCGATT
AATTTTGCTTGCGGTAGTAC

Product: putative exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MQITTVNVNGIRAAVKQRSDANRGMLPWLEQCGSDVVLLQEVRASEKDSLTALAPALDAGWHYVGAPAAAKGRAGVGILS
RQPLRDVQVGLEGFVDSGRYIQATLSDPDTGHDVVVASLYLPSGSADTDKQDEKYRFLDVFGPFLEQAAQDNPHMVVGGD
WNICHRMQDLKNWKTNRKKSGFLPDERAFMDSVFGVFPDSESQVSDAGQWAGAVDYTSQQRRTPANDPKWFDVARRLAPE
DAAYTWWTYRGQAFDTNAGWRIDYQAATSAMLERATRCWVDKAAAYDLRWSDHSPLTVVYS

Sequences:

>Translated_301_residues
MQITTVNVNGIRAAVKQRSDANRGMLPWLEQCGSDVVLLQEVRASEKDSLTALAPALDAGWHYVGAPAAAKGRAGVGILS
RQPLRDVQVGLEGFVDSGRYIQATLSDPDTGHDVVVASLYLPSGSADTDKQDEKYRFLDVFGPFLEQAAQDNPHMVVGGD
WNICHRMQDLKNWKTNRKKSGFLPDERAFMDSVFGVFPDSESQVSDAGQWAGAVDYTSQQRRTPANDPKWFDVARRLAPE
DAAYTWWTYRGQAFDTNAGWRIDYQAATSAMLERATRCWVDKAAAYDLRWSDHSPLTVVYS
>Mature_301_residues
MQITTVNVNGIRAAVKQRSDANRGMLPWLEQCGSDVVLLQEVRASEKDSLTALAPALDAGWHYVGAPAAAKGRAGVGILS
RQPLRDVQVGLEGFVDSGRYIQATLSDPDTGHDVVVASLYLPSGSADTDKQDEKYRFLDVFGPFLEQAAQDNPHMVVGGD
WNICHRMQDLKNWKTNRKKSGFLPDERAFMDSVFGVFPDSESQVSDAGQWAGAVDYTSQQRRTPANDPKWFDVARRLAPE
DAAYTWWTYRGQAFDTNAGWRIDYQAATSAMLERATRCWVDKAAAYDLRWSDHSPLTVVYS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=301, Percent_Identity=28.2392026578073, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI18375503, Length=301, Percent_Identity=28.2392026578073, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI18375501, Length=301, Percent_Identity=28.2392026578073, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 33441; Mature: 33441

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITTVNVNGIRAAVKQRSDANRGMLPWLEQCGSDVVLLQEVRASEKDSLTALAPALDAG
CEEEEEECHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEHHHHHCCCCCCHHHHHHHHHCC
WHYVGAPAAAKGRAGVGILSRQPLRDVQVGLEGFVDSGRYIQATLSDPDTGHDVVVASLY
CEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEE
LPSGSADTDKQDEKYRFLDVFGPFLEQAAQDNPHMVVGGDWNICHRMQDLKNWKTNRKKS
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCC
GFLPDERAFMDSVFGVFPDSESQVSDAGQWAGAVDYTSQQRRTPANDPKWFDVARRLAPE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHCCC
DAAYTWWTYRGQAFDTNAGWRIDYQAATSAMLERATRCWVDKAAAYDLRWSDHSPLTVVY
CCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEE
S
C
>Mature Secondary Structure
MQITTVNVNGIRAAVKQRSDANRGMLPWLEQCGSDVVLLQEVRASEKDSLTALAPALDAG
CEEEEEECHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEHHHHHCCCCCCHHHHHHHHHCC
WHYVGAPAAAKGRAGVGILSRQPLRDVQVGLEGFVDSGRYIQATLSDPDTGHDVVVASLY
CEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEE
LPSGSADTDKQDEKYRFLDVFGPFLEQAAQDNPHMVVGGDWNICHRMQDLKNWKTNRKKS
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCC
GFLPDERAFMDSVFGVFPDSESQVSDAGQWAGAVDYTSQQRRTPANDPKWFDVARRLAPE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHCCC
DAAYTWWTYRGQAFDTNAGWRIDYQAATSAMLERATRCWVDKAAAYDLRWSDHSPLTVVY
CCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEE
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]