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The map label for this gene is metZ [H]

Identifier: 38233237

GI number: 38233237

Start: 598958

End: 600274

Strand: Reverse

Name: metZ [H]

Synonym: DIP0630

Alternate gene names: 38233237

Gene position: 600274-598958 (Counterclockwise)

Preceding gene: 38233238

Following gene: 38233231

Centisome position: 24.12

GC content: 55.66

Gene sequence:

>1317_bases
ATGCCAACAAAATACGATAATTCCAATGCCAACAAATGGGGTTTCGAGACTCGCTCCATCCACGCAGGACAAAGCGTCGA
TAGTGATACCAGTGCCCGCAACCTACCGATTTACCTGACATCATCGTACGTTTTTAATGACGCCGAACACGCAGCAAACC
GCTTCAACCTTTCCGACGCCGGCCCGGTTTACTCTCGCCTGACCAACCCAACTGTCGCGGCAGTCGAAGAACGCCTAGCC
AATCTTGAAGGTGGCGTACACGCCGTACTTTTCGCTTCCGGAATGGCCGCCGAAACCGCCGCAATCCTCAACATCGCCCG
CGCGGGTTCCCACATCGTGTCCAGTCCTCGCATTTACGGCGGCACCGAAACACTCTTTGCCGTCACATTGGCACGCCTGG
GCATCGAAACCACTTTCGTAGAAAATCCTGACGACCCAGCCTCATGGGAGGCTGCAGTTCAAGACAACACGGTAGCTCTC
TACGGAGAAACCTTCGCTAATCCACAAGCAGACGTGCTTGATATTCCCGCAATCGCAGAGGTTGCCCATAAACATCAAGT
ACCACTGATCGTCGACAACACCCTCGCAACCGCAGCCCTTGTACGCCCCCTCGAACTCGGTGCAGACGTCGTCGTGGCAT
CCCTAACCAAGTTCTACACCGGAAATGGCTCCGGACTCGGCGGAGTGCTTATCGACGGCGGAAACTTCGACTGGACCGTC
ACACGCAACGGCGAACCGATCTTCCCCGACTTTGTCACCCCAGATCCCGCCTATCACGGTCTCAAGTATTCCGATCTTGG
TGCCCCCGCCTTCGGACTAAAGGCTCGCGTCGGACTCCTGCGCGACACCGGCGCAGCCCCATCACCACTCAACGCATGGA
TCACCGCACAAGGGCTCGACACCCTCTCGCTACGAGTACAACGCCACAACGAAAACGCACTCGCAGTAGCACAATTCCTC
GCCAACCACGAGAAAGTAGCCAAGGTTAACTACGCAGGCCTTCCCGACTCCCCTTGGTACCCAGTCAAAGAAAAACTCGG
ATTCGACTACACCGGCTCCGTACTTTCCTTTGACGTTAAAGGTGGAAAAAACGAAGCATGGCGCTTTATCGACGCACTCA
AACTACACTCGAACCTCGCCAACGTCGGAGACGTACGTTCCCTCGTAGTCCACCCAGCGACCACCACGCACTCACAATCG
GAAGAATCGGCACTTCTAGCCGCAGGAATTAATCAAGCAACCATCCGACTCTCCGTCGGCATCGAATCCATCGACGACAT
CATCGCCGACCTCACAGCAGGTTTCGACGCAATCTAA

Upstream 100 bases:

>100_bases
TGGACTTTTAGAATTTTATAGACCGATCGGTTTCTTTTTCCCTGAAGTCGCCCTAGCGTTGCTTGCAACGAAAACATCTT
AGGGAAGGAATCGACGATTC

Downstream 100 bases:

>100_bases
ACCTAGACCCGAATGACTAAGAGGGGGTCTGCTGCCACCAACGACAGCAGACCCCCCTCTTAGTCATTCACGCATGGGAG
ATTCTGGAAGAAATCGACAA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: L-methionine; acetate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPTKYDNSNANKWGFETRSIHAGQSVDSDTSARNLPIYLTSSYVFNDAEHAANRFNLSDAGPVYSRLTNPTVAAVEERLA
NLEGGVHAVLFASGMAAETAAILNIARAGSHIVSSPRIYGGTETLFAVTLARLGIETTFVENPDDPASWEAAVQDNTVAL
YGETFANPQADVLDIPAIAEVAHKHQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGNFDWTV
TRNGEPIFPDFVTPDPAYHGLKYSDLGAPAFGLKARVGLLRDTGAAPSPLNAWITAQGLDTLSLRVQRHNENALAVAQFL
ANHEKVAKVNYAGLPDSPWYPVKEKLGFDYTGSVLSFDVKGGKNEAWRFIDALKLHSNLANVGDVRSLVVHPATTTHSQS
EESALLAAGINQATIRLSVGIESIDDIIADLTAGFDAI

Sequences:

>Translated_438_residues
MPTKYDNSNANKWGFETRSIHAGQSVDSDTSARNLPIYLTSSYVFNDAEHAANRFNLSDAGPVYSRLTNPTVAAVEERLA
NLEGGVHAVLFASGMAAETAAILNIARAGSHIVSSPRIYGGTETLFAVTLARLGIETTFVENPDDPASWEAAVQDNTVAL
YGETFANPQADVLDIPAIAEVAHKHQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGNFDWTV
TRNGEPIFPDFVTPDPAYHGLKYSDLGAPAFGLKARVGLLRDTGAAPSPLNAWITAQGLDTLSLRVQRHNENALAVAQFL
ANHEKVAKVNYAGLPDSPWYPVKEKLGFDYTGSVLSFDVKGGKNEAWRFIDALKLHSNLANVGDVRSLVVHPATTTHSQS
EESALLAAGINQATIRLSVGIESIDDIIADLTAGFDAI
>Mature_437_residues
PTKYDNSNANKWGFETRSIHAGQSVDSDTSARNLPIYLTSSYVFNDAEHAANRFNLSDAGPVYSRLTNPTVAAVEERLAN
LEGGVHAVLFASGMAAETAAILNIARAGSHIVSSPRIYGGTETLFAVTLARLGIETTFVENPDDPASWEAAVQDNTVALY
GETFANPQADVLDIPAIAEVAHKHQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGNFDWTVT
RNGEPIFPDFVTPDPAYHGLKYSDLGAPAFGLKARVGLLRDTGAAPSPLNAWITAQGLDTLSLRVQRHNENALAVAQFLA
NHEKVAKVNYAGLPDSPWYPVKEKLGFDYTGSVLSFDVKGGKNEAWRFIDALKLHSNLANVGDVRSLVVHPATTTHSQSE
ESALLAAGINQATIRLSVGIESIDDIIADLTAGFDAI

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=409, Percent_Identity=32.2738386308068, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI299473759, Length=409, Percent_Identity=29.0953545232274, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI25453487, Length=408, Percent_Identity=28.4313725490196, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1790375, Length=423, Percent_Identity=30.9692671394799, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789383, Length=437, Percent_Identity=28.604118993135, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=409, Percent_Identity=31.7848410757946, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17538055, Length=413, Percent_Identity=32.6876513317191, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=412, Percent_Identity=31.3106796116505, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=41.5525114155251, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6319307, Length=427, Percent_Identity=33.9578454332553, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321170, Length=361, Percent_Identity=27.1468144044321, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321254, Length=426, Percent_Identity=24.6478873239437, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321904, Length=411, Percent_Identity=26.7639902676399, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=33.9578454332553, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.49

Molecular weight: Translated: 46575; Mature: 46444

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTKYDNSNANKWGFETRSIHAGQSVDSDTSARNLPIYLTSSYVFNDAEHAANRFNLSDA
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCEEECCHHHHHHHCCCCCC
GPVYSRLTNPTVAAVEERLANLEGGVHAVLFASGMAAETAAILNIARAGSHIVSSPRIYG
CHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCEEEC
GTETLFAVTLARLGIETTFVENPDDPASWEAAVQDNTVALYGETFANPQADVLDIPAIAE
CCHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEECCHHHH
VAHKHQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGNFDWTV
HHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEE
TRNGEPIFPDFVTPDPAYHGLKYSDLGAPAFGLKARVGLLRDTGAAPSPLNAWITAQGLD
ECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHEEEECCCCCCCCCCEEEEECCCC
TLSLRVQRHNENALAVAQFLANHEKVAKVNYAGLPDSPWYPVKEKLGFDYTGSVLSFDVK
EEEEEEEECCCCHHHHHHHHHCCHHEEEEEECCCCCCCCCCHHHHCCCCCCCCEEEEEEC
GGKNEAWRFIDALKLHSNLANVGDVRSLVVHPATTTHSQSEESALLAAGINQATIRLSVG
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEC
IESIDDIIADLTAGFDAI
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PTKYDNSNANKWGFETRSIHAGQSVDSDTSARNLPIYLTSSYVFNDAEHAANRFNLSDA
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCEEECCHHHHHHHCCCCCC
GPVYSRLTNPTVAAVEERLANLEGGVHAVLFASGMAAETAAILNIARAGSHIVSSPRIYG
CHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCEEEC
GTETLFAVTLARLGIETTFVENPDDPASWEAAVQDNTVALYGETFANPQADVLDIPAIAE
CCHHHHHHHHHHHCCEEEEECCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEECCHHHH
VAHKHQVPLIVDNTLATAALVRPLELGADVVVASLTKFYTGNGSGLGGVLIDGGNFDWTV
HHHHCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEE
TRNGEPIFPDFVTPDPAYHGLKYSDLGAPAFGLKARVGLLRDTGAAPSPLNAWITAQGLD
ECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHEEEECCCCCCCCCCEEEEECCCC
TLSLRVQRHNENALAVAQFLANHEKVAKVNYAGLPDSPWYPVKEKLGFDYTGSVLSFDVK
EEEEEEEECCCCHHHHHHHHHCCHHEEEEEECCCCCCCCCCHHHHCCCCCCCCEEEEEEC
GGKNEAWRFIDALKLHSNLANVGDVRSLVVHPATTTHSQSEESALLAAGINQATIRLSVG
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEC
IESIDDIIADLTAGFDAI
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: O-acetyl-L-homoserine; methanethiol

Specific reaction: O-acetyl-L-homoserine + methanethiol = L-methionine + acetate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]