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The map label for this gene is folD

Identifier: 38233227

GI number: 38233227

Start: 588393

End: 589238

Strand: Direct

Name: folD

Synonym: DIP0620

Alternate gene names: 38233227

Gene position: 588393-589238 (Clockwise)

Preceding gene: 38233226

Following gene: 38233228

Centisome position: 23.64

GC content: 50.24

Gene sequence:

>846_bases
GTGAGTGCTATCAAACTTGACGGAAACTTGTATCGCGACGAAATCTTCGAAGATCTTAAAGGACGTGTCAATTCTCTCCG
AGAGAAGGGGATCGTGCCAGGTCTTGCGACTGTTCTTGTCGGCGATGACCCTGCCAGCCACGCCTATGTCCGTATGAAGC
ACAAGGATTGTGAAATTGTTGGTGTGAAGTCGATCCGTAAGGATCTTCCCGCTGATGTGACTCAAGAAGAGCTGTTAGCT
GTTATTGATGATCTAAATGCTGATCCTGAGTGCACTGGCTACATTGTGCAGCTCCCATTGCCAAAGCACTTGGATGAGAA
TGCGGTGCTAGAGCGCATTGATCCTGCGAAAGACGCCGATGGTTTGCACCCAGTGAATCTGGGTAAATTGGTTTTGAATG
AGCCTGCTCCGTTGCCATGTACCCCTAATGGTGCAATTCATCTGTTGCGTCGTTTTGGAGTTGAGCTAGATGGCAAAAAA
GTAGTTGTAATTGGACGTGGTGTGACTGTTGGGCGTCCTATTGGTTTGATGTTGACGCGGAGAAGTGAGAACTCTACCGT
TACGCTGTGTCATACGGGTACTCGTGATCTTGCTGCGGAGACTCGTGAAGCTGATGTCATTGTTGCTGCTGCGGGTAAAG
CGCACATGCTAACGGCTGATATGGTCAAACAGGGTGCTGCGATTCTTGATGTGGGAGTATCGCGTGTTGACGGCAAACTG
CTTGGCGACGTTCACCCTGATGTCTGGGATGTTGCAGGTGCAGTTTCTCCAAATCCTGGTGGGGTTGGACCGCTGACTCG
CGCTTTCTTGATTCGTAACGTAGTAGAGCGTGCTGAGCGTGCCTAG

Upstream 100 bases:

>100_bases
ATTAAATCACCAAAAGTATTTTGCGTATTTTTAACAATGGACCTGCGGTTATGGGAAGGAGTCGTCGATTCTTTCCTCAA
CACATGCAATAATCAAGGCC

Downstream 100 bases:

>100_bases
GCTTTCGCCTTTTGACAATCCGCATGATGTGGGTAGGAAAGAATCGCCGATTCCTTCCTACCTCCAGTACATCGCGGTAG
CGGCGTTTGTAGGAATTGTC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSAIKLDGNLYRDEIFEDLKGRVNSLREKGIVPGLATVLVGDDPASHAYVRMKHKDCEIVGVKSIRKDLPADVTQEELLA
VIDDLNADPECTGYIVQLPLPKHLDENAVLERIDPAKDADGLHPVNLGKLVLNEPAPLPCTPNGAIHLLRRFGVELDGKK
VVVIGRGVTVGRPIGLMLTRRSENSTVTLCHTGTRDLAAETREADVIVAAAGKAHMLTADMVKQGAAILDVGVSRVDGKL
LGDVHPDVWDVAGAVSPNPGGVGPLTRAFLIRNVVERAERA

Sequences:

>Translated_281_residues
MSAIKLDGNLYRDEIFEDLKGRVNSLREKGIVPGLATVLVGDDPASHAYVRMKHKDCEIVGVKSIRKDLPADVTQEELLA
VIDDLNADPECTGYIVQLPLPKHLDENAVLERIDPAKDADGLHPVNLGKLVLNEPAPLPCTPNGAIHLLRRFGVELDGKK
VVVIGRGVTVGRPIGLMLTRRSENSTVTLCHTGTRDLAAETREADVIVAAAGKAHMLTADMVKQGAAILDVGVSRVDGKL
LGDVHPDVWDVAGAVSPNPGGVGPLTRAFLIRNVVERAERA
>Mature_280_residues
SAIKLDGNLYRDEIFEDLKGRVNSLREKGIVPGLATVLVGDDPASHAYVRMKHKDCEIVGVKSIRKDLPADVTQEELLAV
IDDLNADPECTGYIVQLPLPKHLDENAVLERIDPAKDADGLHPVNLGKLVLNEPAPLPCTPNGAIHLLRRFGVELDGKKV
VVIGRGVTVGRPIGLMLTRRSENSTVTLCHTGTRDLAAETREADVIVAAAGKAHMLTADMVKQGAAILDVGVSRVDGKLL
GDVHPDVWDVAGAVSPNPGGVGPLTRAFLIRNVVERAERA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=41.156462585034, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=38.7755102040816, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=36.551724137931, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI36796743, Length=231, Percent_Identity=27.7056277056277, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=42.2939068100358, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=36.734693877551, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319558, Length=265, Percent_Identity=41.5094339622642, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=36.6666666666667, Blast_Score=177, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=40.893470790378, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=40.893470790378, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI62472483, Length=294, Percent_Identity=40.4761904761905, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI45551871, Length=294, Percent_Identity=40.4761904761905, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24645718, Length=294, Percent_Identity=40.4761904761905, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI17137370, Length=294, Percent_Identity=40.4761904761905, Blast_Score=194, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CORDI (Q6NIZ6)

Other databases:

- EMBL:   BX248355
- RefSeq:   NP_938994.1
- HSSP:   O50385
- ProteinModelPortal:   Q6NIZ6
- SMR:   Q6NIZ6
- GeneID:   2650470
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0620
- NMPDR:   fig|257309.1.peg.585
- HOGENOM:   HBG328751
- OMA:   CTPLGCI
- ProtClustDB:   PRK14193
- BioCyc:   CDIP257309:DIP0620-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30086; Mature: 29955

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIKLDGNLYRDEIFEDLKGRVNSLREKGIVPGLATVLVGDDPASHAYVRMKHKDCEIV
CCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEE
GVKSIRKDLPADVTQEELLAVIDDLNADPECTGYIVQLPLPKHLDENAVLERIDPAKDAD
EHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
GLHPVNLGKLVLNEPAPLPCTPNGAIHLLRRFGVELDGKKVVVIGRGVTVGRPIGLMLTR
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEE
RSENSTVTLCHTGTRDLAAETREADVIVAAAGKAHMLTADMVKQGAAILDVGVSRVDGKL
CCCCCEEEEEECCCHHHHHCCCCCCEEEEECCCCCEEEHHHHHCCCEEEECCHHHHCCCC
LGDVHPDVWDVAGAVSPNPGGVGPLTRAFLIRNVVERAERA
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAIKLDGNLYRDEIFEDLKGRVNSLREKGIVPGLATVLVGDDPASHAYVRMKHKDCEIV
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCEEE
GVKSIRKDLPADVTQEELLAVIDDLNADPECTGYIVQLPLPKHLDENAVLERIDPAKDAD
EHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCC
GLHPVNLGKLVLNEPAPLPCTPNGAIHLLRRFGVELDGKKVVVIGRGVTVGRPIGLMLTR
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEE
RSENSTVTLCHTGTRDLAAETREADVIVAAAGKAHMLTADMVKQGAAILDVGVSRVDGKL
CCCCCEEEEEECCCHHHHHCCCCCCEEEEECCCCCEEEHHHHHCCCEEEECCHHHHCCCC
LGDVHPDVWDVAGAVSPNPGGVGPLTRAFLIRNVVERAERA
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910