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The map label for this gene is aglA [H]

Identifier: 38233141

GI number: 38233141

Start: 498593

End: 500293

Strand: Reverse

Name: aglA [H]

Synonym: DIP0532

Alternate gene names: 38233141

Gene position: 500293-498593 (Counterclockwise)

Preceding gene: 38233142

Following gene: 38233140

Centisome position: 20.1

GC content: 55.91

Gene sequence:

>1701_bases
ATGGATACTAGAGACCCCCATGCTTGGTGGCGTGACGCTGCCATTTACCAGATTTACCCCAAGTCCTTCGCTTCGTCGGG
TGGACCTATGGGAACGCTTCGGGGAATCACCTCTCGGTTGGATTATGTTCGTGACCTTGGCGTAGACGCCATCTGGTTAT
CACCGTTTTACACTTCTCCGCAGCGTGATGGCGGTTATGATGTTGCTGATTATTTCAGCGTTGATCCGCTGTTCGGTTCC
AACGCCGATGCGGAGGAGCTCATTTCTGAGGCTCATGACCGTGGCCTGCGGGTGATTTTCGATCTCGTTCCTAATCACAC
TTCCGATCAGCACGTGTGGTTTCGTGAAGCATTACAAGCGGGGCCTGGTTCTCCGAAGCGCAATCATTATTGGTTCCGCG
AGGGCAAGGGGCCGCAAGGCTGCGAGCCTCCTAACGATTGGTTGTCTATTTTCGGCGGGAGCGCATGGACTCAGGTATGT
GCGCGTGACGACGCCCCCGATTCCCCGTGGGAGCACGATACCTCGTGGTACCTGCACTTATTTGACTCTTCACAGCCGGA
TCTTAATTGGTCAAACAAAGATGTGGTCGAGTTTTTTGATAGCATTCTTCGTTTTTGGCTTGATCGCGGAGTCGATGGTT
TCCGCGTTGATGTAGCCCATGGCCTTGCCAAGGACCCAGAGCTGCCCGACTGGCAGTTCCACTGGACAATGGTTGATGGT
GGGCACGACAGCCCCGAGGACGTTCCGCCACCACCGATGTGGAACCGCGAAGAAGTCCATCAGATCTACCGCCATTGGCG
CACGGTTCTGGATGAATACCCAGGTAATCGCGCCTTGGTTGCTGAGGCTTGGGTTGATGCGACAACTGATATTGCTCATT
ATGTTCAGCCGGATGAGATGAACCAAGCGTTTAATTTTGATTTCCTCATCTGTCCATGGCGTTCCGAGGATCTTGGGCGT
GTTATTCATACGTCCTTGGAGGCTCTTGCTCCTACGGGGTCGCCGGCGACGTGGGTGATGTCGAACCATGACGTTGTTCG
CGCGTCGTCACGCTTGGGACTTAGTACCCCAGGTGCACGCCCTAACGGTATCCGCGCTACCGACGAGCAACCCGACGCTG
ACCTCGGTGCGCGTCGCGCCCGCGCTGCGCATATGCTGATGTATTCGTTGCCAGGTTCGGTGTACATCTATCAGGGCGAG
GAACTTAATCTGCCAGAGCACACCACGCTTGACGACGCCCTGCGCCAGGATCCCACGTATTTCCGCACCGAGCACCGCGA
AGCCGGCCGTGATGGCTGCCGCATCCCCCTGCCGTGGACGTCGCAACGCCCAGGCCTTGGGTTTTCCCCCACCGGACAAA
CCTGGTTGCCACAGCCAGAAGGCTGGGAGAATCGGGCAGTGTCACATCAAGAGTCGGATCCCCATTCCGATCTCATGCTT
TTTAGGCGCATGCTGCAGGTCCGAAAGTCCTTGAACTTTGGCCGAGGTCGGCTGTCTCCTGTGTGGTTGAAGCAGGATTG
TTTGGCGTACATCAATTCTGATGATGGACGTTGTGACGTGATGCTGATCGTCGCTTTTGACCAGGATGTCGCAGTCCCCG
AAGGCTGGCACATCGCGTTGTCCACAGAGGAATGCCACGATGTTGTTGCAGCTAATAGTGCTGCTTGGCTGCTGCGTGAA
GGAGACTCATGGCACGTATAA

Upstream 100 bases:

>100_bases
TCCGTTGTGGTGATGTGACATATCCCGATCTCTCAGATCACAGCGTGGTTGAGTTGGGTCCTACTTCTGGCGTCATCTTG
TCCGTGTAACAATGTTGTCT

Downstream 100 bases:

>100_bases
CTTCGCTGACCGCTGTTGATTCATTCTTTTGCGAAGACGATCAGTGGCAGCATGGCACACGCCAGCGCACTGTGTTAAAC
GATGAGCAGCTGAGCGTGCT

Product: putative amylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MDTRDPHAWWRDAAIYQIYPKSFASSGGPMGTLRGITSRLDYVRDLGVDAIWLSPFYTSPQRDGGYDVADYFSVDPLFGS
NADAEELISEAHDRGLRVIFDLVPNHTSDQHVWFREALQAGPGSPKRNHYWFREGKGPQGCEPPNDWLSIFGGSAWTQVC
ARDDAPDSPWEHDTSWYLHLFDSSQPDLNWSNKDVVEFFDSILRFWLDRGVDGFRVDVAHGLAKDPELPDWQFHWTMVDG
GHDSPEDVPPPPMWNREEVHQIYRHWRTVLDEYPGNRALVAEAWVDATTDIAHYVQPDEMNQAFNFDFLICPWRSEDLGR
VIHTSLEALAPTGSPATWVMSNHDVVRASSRLGLSTPGARPNGIRATDEQPDADLGARRARAAHMLMYSLPGSVYIYQGE
ELNLPEHTTLDDALRQDPTYFRTEHREAGRDGCRIPLPWTSQRPGLGFSPTGQTWLPQPEGWENRAVSHQESDPHSDLML
FRRMLQVRKSLNFGRGRLSPVWLKQDCLAYINSDDGRCDVMLIVAFDQDVAVPEGWHIALSTEECHDVVAANSAAWLLRE
GDSWHV

Sequences:

>Translated_566_residues
MDTRDPHAWWRDAAIYQIYPKSFASSGGPMGTLRGITSRLDYVRDLGVDAIWLSPFYTSPQRDGGYDVADYFSVDPLFGS
NADAEELISEAHDRGLRVIFDLVPNHTSDQHVWFREALQAGPGSPKRNHYWFREGKGPQGCEPPNDWLSIFGGSAWTQVC
ARDDAPDSPWEHDTSWYLHLFDSSQPDLNWSNKDVVEFFDSILRFWLDRGVDGFRVDVAHGLAKDPELPDWQFHWTMVDG
GHDSPEDVPPPPMWNREEVHQIYRHWRTVLDEYPGNRALVAEAWVDATTDIAHYVQPDEMNQAFNFDFLICPWRSEDLGR
VIHTSLEALAPTGSPATWVMSNHDVVRASSRLGLSTPGARPNGIRATDEQPDADLGARRARAAHMLMYSLPGSVYIYQGE
ELNLPEHTTLDDALRQDPTYFRTEHREAGRDGCRIPLPWTSQRPGLGFSPTGQTWLPQPEGWENRAVSHQESDPHSDLML
FRRMLQVRKSLNFGRGRLSPVWLKQDCLAYINSDDGRCDVMLIVAFDQDVAVPEGWHIALSTEECHDVVAANSAAWLLRE
GDSWHV
>Mature_566_residues
MDTRDPHAWWRDAAIYQIYPKSFASSGGPMGTLRGITSRLDYVRDLGVDAIWLSPFYTSPQRDGGYDVADYFSVDPLFGS
NADAEELISEAHDRGLRVIFDLVPNHTSDQHVWFREALQAGPGSPKRNHYWFREGKGPQGCEPPNDWLSIFGGSAWTQVC
ARDDAPDSPWEHDTSWYLHLFDSSQPDLNWSNKDVVEFFDSILRFWLDRGVDGFRVDVAHGLAKDPELPDWQFHWTMVDG
GHDSPEDVPPPPMWNREEVHQIYRHWRTVLDEYPGNRALVAEAWVDATTDIAHYVQPDEMNQAFNFDFLICPWRSEDLGR
VIHTSLEALAPTGSPATWVMSNHDVVRASSRLGLSTPGARPNGIRATDEQPDADLGARRARAAHMLMYSLPGSVYIYQGE
ELNLPEHTTLDDALRQDPTYFRTEHREAGRDGCRIPLPWTSQRPGLGFSPTGQTWLPQPEGWENRAVSHQESDPHSDLML
FRRMLQVRKSLNFGRGRLSPVWLKQDCLAYINSDDGRCDVMLIVAFDQDVAVPEGWHIALSTEECHDVVAANSAAWLLRE
GDSWHV

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=575, Percent_Identity=27.6521739130435, Blast_Score=213, Evalue=6e-55,
Organism=Homo sapiens, GI65506891, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI61744479, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI61744475, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI61744481, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI61744477, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI61744483, Length=110, Percent_Identity=37.2727272727273, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1790687, Length=522, Percent_Identity=34.2911877394636, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1786604, Length=237, Percent_Identity=29.535864978903, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789995, Length=250, Percent_Identity=28.4, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25147709, Length=506, Percent_Identity=24.3083003952569, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32565753, Length=430, Percent_Identity=23.953488372093, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322245, Length=583, Percent_Identity=31.5608919382504, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321731, Length=527, Percent_Identity=33.2068311195446, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319776, Length=527, Percent_Identity=33.0170777988615, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321726, Length=529, Percent_Identity=31.9470699432892, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6322241, Length=525, Percent_Identity=32, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6322021, Length=525, Percent_Identity=32, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6324416, Length=525, Percent_Identity=32, Blast_Score=233, Evalue=9e-62,
Organism=Drosophila melanogaster, GI24583749, Length=525, Percent_Identity=32.5714285714286, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24583747, Length=525, Percent_Identity=32.5714285714286, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24586597, Length=505, Percent_Identity=32.8712871287129, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI221330053, Length=530, Percent_Identity=33.0188679245283, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24583745, Length=537, Percent_Identity=29.9813780260708, Blast_Score=242, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24586591, Length=522, Percent_Identity=31.992337164751, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24586589, Length=543, Percent_Identity=31.1233885819521, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24586599, Length=543, Percent_Identity=31.3075506445672, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24586593, Length=592, Percent_Identity=31.4189189189189, Blast_Score=235, Evalue=7e-62,
Organism=Drosophila melanogaster, GI45549022, Length=550, Percent_Identity=29.6363636363636, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24586587, Length=517, Percent_Identity=30.174081237911, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI281360393, Length=537, Percent_Identity=29.2364990689013, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 64300; Mature: 64300

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTRDPHAWWRDAAIYQIYPKSFASSGGPMGTLRGITSRLDYVRDLGVDAIWLSPFYTSP
CCCCCCCHHHHCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
QRDGGYDVADYFSVDPLFGSNADAEELISEAHDRGLRVIFDLVPNHTSDQHVWFREALQA
CCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHC
GPGSPKRNHYWFREGKGPQGCEPPNDWLSIFGGSAWTQVCARDDAPDSPWEHDTSWYLHL
CCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEE
FDSSQPDLNWSNKDVVEFFDSILRFWLDRGVDGFRVDVAHGLAKDPELPDWQFHWTMVDG
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCEEEEEEEEC
GHDSPEDVPPPPMWNREEVHQIYRHWRTVLDEYPGNRALVAEAWVDATTDIAHYVQPDEM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHCCCHHH
NQAFNFDFLICPWRSEDLGRVIHTSLEALAPTGSPATWVMSNHDVVRASSRLGLSTPGAR
CHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHCCCCCCCCCCC
PNGIRATDEQPDADLGARRARAAHMLMYSLPGSVYIYQGEELNLPEHTTLDDALRQDPTY
CCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCH
FRTEHREAGRDGCRIPLPWTSQRPGLGFSPTGQTWLPQPEGWENRAVSHQESDPHSDLML
HHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FRRMLQVRKSLNFGRGRLSPVWLKQDCLAYINSDDGRCDVMLIVAFDQDVAVPEGWHIAL
HHHHHHHHHHCCCCCCCCCEEEECHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCEEEE
STEECHDVVAANSAAWLLREGDSWHV
EHHHHHHHHHCCCEEEEEECCCCCCC
>Mature Secondary Structure
MDTRDPHAWWRDAAIYQIYPKSFASSGGPMGTLRGITSRLDYVRDLGVDAIWLSPFYTSP
CCCCCCCHHHHCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
QRDGGYDVADYFSVDPLFGSNADAEELISEAHDRGLRVIFDLVPNHTSDQHVWFREALQA
CCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHC
GPGSPKRNHYWFREGKGPQGCEPPNDWLSIFGGSAWTQVCARDDAPDSPWEHDTSWYLHL
CCCCCCCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEE
FDSSQPDLNWSNKDVVEFFDSILRFWLDRGVDGFRVDVAHGLAKDPELPDWQFHWTMVDG
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCEEEEEEEEC
GHDSPEDVPPPPMWNREEVHQIYRHWRTVLDEYPGNRALVAEAWVDATTDIAHYVQPDEM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHCCCHHH
NQAFNFDFLICPWRSEDLGRVIHTSLEALAPTGSPATWVMSNHDVVRASSRLGLSTPGAR
CHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHCCCCCCCCCCC
PNGIRATDEQPDADLGARRARAAHMLMYSLPGSVYIYQGEELNLPEHTTLDDALRQDPTY
CCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCH
FRTEHREAGRDGCRIPLPWTSQRPGLGFSPTGQTWLPQPEGWENRAVSHQESDPHSDLML
HHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FRRMLQVRKSLNFGRGRLSPVWLKQDCLAYINSDDGRCDVMLIVAFDQDVAVPEGWHIAL
HHHHHHHHHHCCCCCCCCCEEEECHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCEEEE
STEECHDVVAANSAAWLLREGDSWHV
EHHHHHHHHHCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]