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The map label for this gene is act [H]

Identifier: 38232995

GI number: 38232995

Start: 345115

End: 345990

Strand: Reverse

Name: act [H]

Synonym: DIP0379

Alternate gene names: 38232995

Gene position: 345990-345115 (Counterclockwise)

Preceding gene: 38232996

Following gene: 38232994

Centisome position: 13.9

GC content: 56.85

Gene sequence:

>876_bases
ATGGCTGATGGTATTAACTCCACAGTGACGGTTGAACGCTCTGACGGGCCGCGCCACCGCGGAACCGCAGCCGGCATCGG
AGGCCTCGACCTCGATCTGGAAGACGTCACTCACAAGGAGCTCATCGAGGCCCGCCGCACTGGCGACGTCGGCCTTGTGC
ACTCCTGGGAGCTTGTCACCGCTGTTGATGGTCCTGGTACCCGCATGACCATGTTCATGTCCGGATGCCCACTACGATGC
CTTTACTGCCACAACCCCGACACCATGGGGATGAAAGAAGGCACAGTAGAGCGCGTCGAATCGATCGTGAAAAAGATCAA
ACGCTATCGCAACGTGTTTAAGGCCTCCGGCGGCGGATTGACCATCTCCGGCGGTGAACCACTGTTCCAAATCGCCTTTA
CGCGCCGCGTACTCAAAGAGGTACATGATGCCGGTATCCACACCACCATCGACACCTCAGGGTATCTCGGGGCACGGCTC
ACGGACGAGGACTTAGAAAACATCGACCTCGTGCTTCTCGACGTCAAGTCCGGCGATGAGGAAACCTACCATCGTGTTTC
CGGCGGCCGCGAGCTACAACCCACCATTGACTTTGGCAATCGCCTCAATGCGATTGGCAAGCCTGTGTGGATCCGCTTCG
TGCTGGTACCTGGTGTGTCTGATGCACCAGAAAACATCAACAACGTGGCAGACATCGTCGCCCAGTGGAAAGACAACGTC
GAACGCGTTGAGGTGCTGCCTTTCCACAACATGGGTGCCGATAAGTGGCACGAACTCGGCTTGAATTACGAGCTCGAGGA
CACAAAACCACCAACACAAGAATCCGTGGAGTTTGCTAAGGAGACTTTCCGCTCCCGCGGTTTGACCGTCTACTAG

Upstream 100 bases:

>100_bases
TACGTTCCACCACTCTGCGTAAGATGTAACGCCACCACCTGCCTGTACACGGGCAGGTGGTATATTTTTGCCCTAATGAA
GCGAAAGGAATCCCTCTGAT

Downstream 100 bases:

>100_bases
CTACAATGGCCGACGTGACTACAACAAAAGGTTCTCGCGTCGGAAAAGTTTCACTCATCATCGCCCTGATCGTCGTCATC
CTGCTCGTCGTAGCAGAATT

Product: putative oxidoreductase

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MADGINSTVTVERSDGPRHRGTAAGIGGLDLDLEDVTHKELIEARRTGDVGLVHSWELVTAVDGPGTRMTMFMSGCPLRC
LYCHNPDTMGMKEGTVERVESIVKKIKRYRNVFKASGGGLTISGGEPLFQIAFTRRVLKEVHDAGIHTTIDTSGYLGARL
TDEDLENIDLVLLDVKSGDEETYHRVSGGRELQPTIDFGNRLNAIGKPVWIRFVLVPGVSDAPENINNVADIVAQWKDNV
ERVEVLPFHNMGADKWHELGLNYELEDTKPPTQESVEFAKETFRSRGLTVY

Sequences:

>Translated_291_residues
MADGINSTVTVERSDGPRHRGTAAGIGGLDLDLEDVTHKELIEARRTGDVGLVHSWELVTAVDGPGTRMTMFMSGCPLRC
LYCHNPDTMGMKEGTVERVESIVKKIKRYRNVFKASGGGLTISGGEPLFQIAFTRRVLKEVHDAGIHTTIDTSGYLGARL
TDEDLENIDLVLLDVKSGDEETYHRVSGGRELQPTIDFGNRLNAIGKPVWIRFVLVPGVSDAPENINNVADIVAQWKDNV
ERVEVLPFHNMGADKWHELGLNYELEDTKPPTQESVEFAKETFRSRGLTVY
>Mature_290_residues
ADGINSTVTVERSDGPRHRGTAAGIGGLDLDLEDVTHKELIEARRTGDVGLVHSWELVTAVDGPGTRMTMFMSGCPLRCL
YCHNPDTMGMKEGTVERVESIVKKIKRYRNVFKASGGGLTISGGEPLFQIAFTRRVLKEVHDAGIHTTIDTSGYLGARLT
DEDLENIDLVLLDVKSGDEETYHRVSGGRELQPTIDFGNRLNAIGKPVWIRFVLVPGVSDAPENINNVADIVAQWKDNVE
RVEVLPFHNMGADKWHELGLNYELEDTKPPTQESVEFAKETFRSRGLTVY

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=38.6831275720165, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1790389, Length=280, Percent_Identity=27.5, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI226510931, Length=197, Percent_Identity=30.9644670050761, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 32219; Mature: 32088

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGINSTVTVERSDGPRHRGTAAGIGGLDLDLEDVTHKELIEARRTGDVGLVHSWELVT
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEE
AVDGPGTRMTMFMSGCPLRCLYCHNPDTMGMKEGTVERVESIVKKIKRYRNVFKASGGGL
EECCCCCEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
TISGGEPLFQIAFTRRVLKEVHDAGIHTTIDTSGYLGARLTDEDLENIDLVLLDVKSGDE
EECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCCCCEEEEEECCCCCH
ETYHRVSGGRELQPTIDFGNRLNAIGKPVWIRFVLVPGVSDAPENINNVADIVAQWKDNV
HHHHHHCCCCEECCCCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
ERVEVLPFHNMGADKWHELGLNYELEDTKPPTQESVEFAKETFRSRGLTVY
CEEEEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADGINSTVTVERSDGPRHRGTAAGIGGLDLDLEDVTHKELIEARRTGDVGLVHSWELVT
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEE
AVDGPGTRMTMFMSGCPLRCLYCHNPDTMGMKEGTVERVESIVKKIKRYRNVFKASGGGL
EECCCCCEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
TISGGEPLFQIAFTRRVLKEVHDAGIHTTIDTSGYLGARLTDEDLENIDLVLLDVKSGDE
EECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEECCCCCCCCEEEEEECCCCCH
ETYHRVSGGRELQPTIDFGNRLNAIGKPVWIRFVLVPGVSDAPENINNVADIVAQWKDNV
HHHHHHCCCCEECCCCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
ERVEVLPFHNMGADKWHELGLNYELEDTKPPTQESVEFAKETFRSRGLTVY
CEEEEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]