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The map label for this gene is alkB [H]

Identifier: 38232967

GI number: 38232967

Start: 311422

End: 312120

Strand: Direct

Name: alkB [H]

Synonym: DIP0349

Alternate gene names: 38232967

Gene position: 311422-312120 (Clockwise)

Preceding gene: 38232965

Following gene: 38232969

Centisome position: 12.51

GC content: 60.8

Gene sequence:

>699_bases
ATGTTGTTTGATTCTCTGCCGCGCCCGAGTGAGCGCATCGCGCCGGGCGTAGCGCACCTGCCGGGCTGGTTGGGCGTCGA
CAAGCAAGCGGAGCTAGTGCGCGAGATCCGCGAGATCGCCCGCACATACGCGGATACTCCGATGGCGATGCTGCGCCCGA
CGCTAAAATCCGGCGGGCAAATGAGCGTGTATCAGCTGCATTTAGGCAGGTACTGGCATTATCCGAGCTACCGGTACATC
GACAAGATTGCGGGTACCACCGTCCCGCCAGTGCCAGACAGCCTAGCTGCGTTGGCGCCGGAAGCGTTGCGCGCAGCAGC
GGAGGTTGCCGAAGAACTAGCCCCGTGGGTAGAGACCTTCGTGCCAGAGATGGTGCTGGTCAACTACTATCCGCCGGGTT
CCAGGATGGGCATGCACGTCGATGAATTTGAGGAATCGCGGGCGCCGGTCATTTCAGTATCGATTGGCGACGAAGCACTG
TTTCGGATGGGACACACCGAATCACGCACCCAACCCTGGGACGATGTGACTCTATGCTCCGGCGACCTGGTGGTTTTCGG
TGGGCCGAAGCGTTTTGCTTATCACGGGGTGGTGCGCGTGAATGATGCCACGCTTCCCGACGGCTGCGGGCTGGAACAAG
GCAGGATTAACATCACGATCCGGCAGGTTTCTGCGGCGCCGAACGTACAGGGGCGTTAA

Upstream 100 bases:

>100_bases
GGGGCGCGCTCGTCGCCGGGGAGCCGACAGCTGAGCAGTTGCTGCCCGCTGTGGGTTGCCACCCATCGACACAGCCCACA
GCAGCCGATACGGTGAGAGC

Downstream 100 bases:

>100_bases
ACAGGGATTTGCTCAAAGATTACCTAGGGAACCGTGGATGCAGACTTGCTAAGAAGACGACAGACCCAGGCTGGAGCCAC
CGCCACCAGCACCGCTACGG

Product: putative alkylated DNA repair protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MLFDSLPRPSERIAPGVAHLPGWLGVDKQAELVREIREIARTYADTPMAMLRPTLKSGGQMSVYQLHLGRYWHYPSYRYI
DKIAGTTVPPVPDSLAALAPEALRAAAEVAEELAPWVETFVPEMVLVNYYPPGSRMGMHVDEFEESRAPVISVSIGDEAL
FRMGHTESRTQPWDDVTLCSGDLVVFGGPKRFAYHGVVRVNDATLPDGCGLEQGRINITIRQVSAAPNVQGR

Sequences:

>Translated_232_residues
MLFDSLPRPSERIAPGVAHLPGWLGVDKQAELVREIREIARTYADTPMAMLRPTLKSGGQMSVYQLHLGRYWHYPSYRYI
DKIAGTTVPPVPDSLAALAPEALRAAAEVAEELAPWVETFVPEMVLVNYYPPGSRMGMHVDEFEESRAPVISVSIGDEAL
FRMGHTESRTQPWDDVTLCSGDLVVFGGPKRFAYHGVVRVNDATLPDGCGLEQGRINITIRQVSAAPNVQGR
>Mature_232_residues
MLFDSLPRPSERIAPGVAHLPGWLGVDKQAELVREIREIARTYADTPMAMLRPTLKSGGQMSVYQLHLGRYWHYPSYRYI
DKIAGTTVPPVPDSLAALAPEALRAAAEVAEELAPWVETFVPEMVLVNYYPPGSRMGMHVDEFEESRAPVISVSIGDEAL
FRMGHTESRTQPWDDVTLCSGDLVVFGGPKRFAYHGVVRVNDATLPDGCGLEQGRINITIRQVSAAPNVQGR

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Escherichia coli, GI1788541, Length=202, Percent_Identity=32.6732673267327, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25615; Mature: 25615

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFDSLPRPSERIAPGVAHLPGWLGVDKQAELVREIREIARTYADTPMAMLRPTLKSGGQ
CCCCCCCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
MSVYQLHLGRYWHYPSYRYIDKIAGTTVPPVPDSLAALAPEALRAAAEVAEELAPWVETF
EEEEEEECCCEECCCCHHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VPEMVLVNYYPPGSRMGMHVDEFEESRAPVISVSIGDEALFRMGHTESRTQPWDDVTLCS
CCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCCCCCEEEEC
GDLVVFGGPKRFAYHGVVRVNDATLPDGCGLEQGRINITIRQVSAAPNVQGR
CCEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MLFDSLPRPSERIAPGVAHLPGWLGVDKQAELVREIREIARTYADTPMAMLRPTLKSGGQ
CCCCCCCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
MSVYQLHLGRYWHYPSYRYIDKIAGTTVPPVPDSLAALAPEALRAAAEVAEELAPWVETF
EEEEEEECCCEECCCCHHHHHHHCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VPEMVLVNYYPPGSRMGMHVDEFEESRAPVISVSIGDEALFRMGHTESRTQPWDDVTLCS
CCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECHHHHHHCCCCCCCCCCCCCEEEEC
GDLVVFGGPKRFAYHGVVRVNDATLPDGCGLEQGRINITIRQVSAAPNVQGR
CCEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9150207 [H]