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The map label for this gene is nth [H]

Identifier: 38232925

GI number: 38232925

Start: 271954

End: 272709

Strand: Direct

Name: nth [H]

Synonym: DIP0304

Alternate gene names: 38232925

Gene position: 271954-272709 (Clockwise)

Preceding gene: 38232923

Following gene: 38232926

Centisome position: 10.93

GC content: 56.22

Gene sequence:

>756_bases
ATGAGTAGACCTGACCTCGCCCAGCAGCCCACCGCACTGGCGGTCAAGCGTCGCGCACGCGCCATCAATCGGGAACTGGC
CAAAGCGTATCCCGACGCCCACTGTGAGCTGGACTTTAACAATCCACTCGAACTCACCGTCGCCACGGTGCTCAGCGCCC
AATGCACCGACGTACGCGTCAATCAAATCACCCCAGCACTATTTGCCAAATATCCCACCGCAGAGGCCTACGCGAGCGCC
AACGAAGCCGAACTGCAAGAAATGATCCGACCCACCGGTTTTTATAAAGCAAAGGCCGCACATCTTATCGGTATGGGGCA
AAAACTGGTCACAGACTTCAGCGGTGAGATCCCACGTGATCTAGAAAGCCTTGTCAGCCTGCCAGGAGTAGGAAGAAAAA
CCGCCCATGTAGTGCGCGGTAACGCCTTTGACATCCCTGGGTTGACCGTCGATACGCACTTCGGACGACTCGTGCGACGC
CTAGGGCTTACTACCCAGACCAACCCCGTGAAAGTCGAACACGAGATAGCCGATCTGATCGAGAAAAAAGAATGGACCAT
GTTCTCACATCGGATCATCTTCCATGGACGTCGCGTATGCCATTCCCGCACCGCTGCATGTGGAGCTTGCTTCCTTGCGC
CGCGCTGCCCAAGCTACGGGGAAGCAGGACCCACAGATCCACTGCGCGCAGAAACTCTTGTCACCGGAGACAACCGCGAA
CACCTACTTCAGATGGCAGGTATTGGAAATGCGTAA

Upstream 100 bases:

>100_bases
CACAACAAAAAAGCCCCCAACACAAGCGGGACCACAACAGCGAGCGCAAGAATCATAGGCATAGCCTAGCGCACTACCAA
CTCACGTTAAGGTAGAGGAC

Downstream 100 bases:

>100_bases
AACAATCGTGGTGTCTGCCCTTGTCTTCGTCGCAGTCTTTGCCGCCCTCATCAGCATGATCGTGGGAAATAACAATGAAC
CCACGCCCGAGAACCTCCAA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSRPDLAQQPTALAVKRRARAINRELAKAYPDAHCELDFNNPLELTVATVLSAQCTDVRVNQITPALFAKYPTAEAYASA
NEAELQEMIRPTGFYKAKAAHLIGMGQKLVTDFSGEIPRDLESLVSLPGVGRKTAHVVRGNAFDIPGLTVDTHFGRLVRR
LGLTTQTNPVKVEHEIADLIEKKEWTMFSHRIIFHGRRVCHSRTAACGACFLAPRCPSYGEAGPTDPLRAETLVTGDNRE
HLLQMAGIGNA

Sequences:

>Translated_251_residues
MSRPDLAQQPTALAVKRRARAINRELAKAYPDAHCELDFNNPLELTVATVLSAQCTDVRVNQITPALFAKYPTAEAYASA
NEAELQEMIRPTGFYKAKAAHLIGMGQKLVTDFSGEIPRDLESLVSLPGVGRKTAHVVRGNAFDIPGLTVDTHFGRLVRR
LGLTTQTNPVKVEHEIADLIEKKEWTMFSHRIIFHGRRVCHSRTAACGACFLAPRCPSYGEAGPTDPLRAETLVTGDNRE
HLLQMAGIGNA
>Mature_250_residues
SRPDLAQQPTALAVKRRARAINRELAKAYPDAHCELDFNNPLELTVATVLSAQCTDVRVNQITPALFAKYPTAEAYASAN
EAELQEMIRPTGFYKAKAAHLIGMGQKLVTDFSGEIPRDLESLVSLPGVGRKTAHVVRGNAFDIPGLTVDTHFGRLVRRL
GLTTQTNPVKVEHEIADLIEKKEWTMFSHRIIFHGRRVCHSRTAACGACFLAPRCPSYGEAGPTDPLRAETLVTGDNREH
LLQMAGIGNA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=26.4044943820225, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787920, Length=186, Percent_Identity=36.0215053763441, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17554540, Length=136, Percent_Identity=37.5, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=198, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319304, Length=149, Percent_Identity=28.8590604026846, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.0734463276836, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27525; Mature: 27394

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPDLAQQPTALAVKRRARAINRELAKAYPDAHCELDFNNPLELTVATVLSAQCTDVRV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEH
NQITPALFAKYPTAEAYASANEAELQEMIRPTGFYKAKAAHLIGMGQKLVTDFSGEIPRD
HHCCHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCCCHH
LESLVSLPGVGRKTAHVVRGNAFDIPGLTVDTHFGRLVRRLGLTTQTNPVKVEHEIADLI
HHHHHCCCCCCCCHHEEEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCCEEHHHHHHHHH
EKKEWTMFSHRIIFHGRRVCHSRTAACGACFLAPRCPSYGEAGPTDPLRAETLVTGDNRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEECCCHH
HLLQMAGIGNA
HHHHHHCCCCC
>Mature Secondary Structure 
SRPDLAQQPTALAVKRRARAINRELAKAYPDAHCELDFNNPLELTVATVLSAQCTDVRV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEH
NQITPALFAKYPTAEAYASANEAELQEMIRPTGFYKAKAAHLIGMGQKLVTDFSGEIPRD
HHCCHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCCCHH
LESLVSLPGVGRKTAHVVRGNAFDIPGLTVDTHFGRLVRRLGLTTQTNPVKVEHEIADLI
HHHHHCCCCCCCCHHEEEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCCEEHHHHHHHHH
EKKEWTMFSHRIIFHGRRVCHSRTAACGACFLAPRCPSYGEAGPTDPLRAETLVTGDNRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEECCCHH
HLLQMAGIGNA
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]