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The map label for this gene is pbpF [H]

Identifier: 38232919

GI number: 38232919

Start: 266238

End: 268622

Strand: Reverse

Name: pbpF [H]

Synonym: DIP0298

Alternate gene names: 38232919

Gene position: 268622-266238 (Counterclockwise)

Preceding gene: 38232924

Following gene: 38232917

Centisome position: 10.79

GC content: 59.71

Gene sequence:

>2385_bases
ATGTGGAATTCCCTGAAAGATATCGTCCTAGCAACGGTCGCCGGCGGCGTGGTATGTGCACTCGCGCTCACCCCTGTGGC
CGCTATTGCTGGCGTGGCCGTCGACCGTACAAACGACACAATGCAGTCCAACCTCCAAGACCTCACGGATGGTTCTGCAC
CTGGCGTTTCTACGATTTTGGACAACCGCAACAACCCCATCGCATGGGTGTATAAGCAGCGCAGGTTCGAAATCCCTTCC
GAAGCCATCCCGCAGTCCATGAAAGACGCCATCGTCTCCATCGAGGACCGCCGCTTCTACGAACACAAAGGTGTGGATAT
TCAGGGCAACCTCCGCGCCATTGTGACCAATATCTTCGCCGGTGGTGTCGCACAAGGAGCCTCCACCATCGACCAGCAGT
ACGTGAAGAACTTCCTCCTCTTGGTCAAGTCCGACAATCCCGAGGAGCAGGCAGCCGCAACGGAGACATCGATTCCCCGC
AAGCTGCGCGAAATGCGCATGGCTAGCACCATCGATTCCACATTAAGCAAAGACGACATACTCACCCGCTACCTCAACCT
CGTCCACCTAGGCAACGGTGCCTTCGGCGTCGAGGCCGCAGCCCGCACCTATTTTGGCAAAGGTGCACCAGAGCTCAGCC
TTGCGCAGTCGGCCATGTTCGCAGGCATTGTGCAGTCCTCGTCCTACTTGGATCCCTACACCAACCCCGACGCGGTGATG
GATCGACGCAACGTAGTGCTCGATACTATGGCGGCCAACGGCTACGCCACCCCCGAGGACGTCGCAGCGGCCAAAGCCGA
ACCCCTCGGCGTGCTCGAGCAACCACAGGGACTACCCAATGGATGCATCACTGCTGGTGACAACGGATTCTTCTGCGACT
ACGTGCTGAAATACCTCAGCGAAAAAGGCTTGAGCACCGACCAGCTCACCCAAGGGTCCTACACCATCAACACCACATTG
GACCCCGACGTACAGCAGGCCGCGCACGCCGCCGCAGCAGGTCAGGTCAATTCGCACACCTTCGGTGTTGCCAATGTGAT
GAACATTGTCGAGCCTGGAAAAGACAGTCGTAAGATCCTCGCGATGACGTCGTCACGCGACTACGGCCTCGACCTCGACG
CCGGCCAAACCGTCCTTCCGCAACCCACCTCCCTGGTAGGCAACGGTGCTGGCTCAGTGTTCAAGATCTTTACCGCCGCG
GTCGCCCTCCAGCAGGGCTACGGTCTGGACACCCAACTCCAAGTTCCACCACGCTACGAGGCCAAAGGCATGGGCGACGG
CGGTGCCGAGGGCTGCGGCGCCGGCACCTACTGTGTAGAAAACTCCGGCAGCTACGCACCACAAATGTCGCTACGCGACG
CCCTCGCGTTCTCCCCGAACACACCATTCGTGCAAATGATTGAGAAAGTCGGCGTTAAAGACGTAGTAGACACCTCCGTC
AAACTCGGACTACGCAGCTACGCCAACCCCGGCACCTTCAACAAAGACAACTCGATCGCCGACTACGTCAAAGAGCACAA
CCTCGGCTCTTACACCCTCGGCCCCACCGCCGTAAACCCCCTCGAACTGTCCAACGTTGCAGCCTCTCTCGCCTCCGACG
GCATGTGGTGCGAACCAAGCCCCATCACCACCGTTACCGACGCCAAAGGCAACGAGGTATTCCTCGACCGCCCAGCCTGC
GAACAAGCCATCGACCCAGAAATCGCCCAAGCACTCAGCGCAGGGCTAAGCCAAGACACCATCAAAGGAACCGGCGCACA
AGCTGCCAAATCCCTAGGATTTAGCGCCCCAACAGCAGCGAAAACCGGCACCACCGAATCGCACTCCTCTGCTGCCTTCC
TCGGATTTAACTCCAACTTCGCTGCAGCGTCCTACATCTACAACGACGGCACCACTGTCACCCCACTGTGCACCTCCCCT
GTCGTACAGTGCGGCGAGGGCACCCTCTTCGGTGGTGACGAACCCGCACGCACATGGTTCACCGCAGCACAAAGCTCCGG
CCTCGCTGCCAGTGGAAACCTGCCAAGCTACGACCCCAAGTTCAATCGAGGTGTCTCGGGATCCTTCGGCACCTCCTATG
TCGGCCGCGAGGGCGAATCAGTGCGCAAAGAACTCAGCGCCAAAGGCTATAAAGTCACCATGACCTCCGCCCCAGGAGAC
GGCATCCCCAAAGGCTCCGTGGTCAACGTGATCGCCCCCGTCCCCCTCAAACCAGGCGGCACGATCGAACTTTTCATTTC
CGACGGCACCCGCCCACGCACCACGCCCCCCTCAACAACTCGACGCCCCAACCCCACCGAGGAGGTCGTCCCAGGAACCG
GGGTGAGCCAGAACGACCTTGACGATCTAGCTCACCAGCTCCAACAGCTCCTGCCCGGTTTCTAG

Upstream 100 bases:

>100_bases
GTGTATTCACAGCAAACCTTCTAAGTCGAATGAGTCACACTTTGAGGGGAGTTTTCCACTTCCCCCACGGGCAGTCCTTA
GCTAGGGTAAGGGAGTGTCA

Downstream 100 bases:

>100_bases
CAGATTAGGCAAGCGCCGCACGAACAGCCGTGGATAGGCGCTTGCCATCGGCACGACCTGCAGCCTGCGCAGTGGCCAGC
TTCATAACCTGGCCCATCTG

Product: putative penicillin-binding secreted protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 794; Mature: 794

Protein sequence:

>794_residues
MWNSLKDIVLATVAGGVVCALALTPVAAIAGVAVDRTNDTMQSNLQDLTDGSAPGVSTILDNRNNPIAWVYKQRRFEIPS
EAIPQSMKDAIVSIEDRRFYEHKGVDIQGNLRAIVTNIFAGGVAQGASTIDQQYVKNFLLLVKSDNPEEQAAATETSIPR
KLREMRMASTIDSTLSKDDILTRYLNLVHLGNGAFGVEAAARTYFGKGAPELSLAQSAMFAGIVQSSSYLDPYTNPDAVM
DRRNVVLDTMAANGYATPEDVAAAKAEPLGVLEQPQGLPNGCITAGDNGFFCDYVLKYLSEKGLSTDQLTQGSYTINTTL
DPDVQQAAHAAAAGQVNSHTFGVANVMNIVEPGKDSRKILAMTSSRDYGLDLDAGQTVLPQPTSLVGNGAGSVFKIFTAA
VALQQGYGLDTQLQVPPRYEAKGMGDGGAEGCGAGTYCVENSGSYAPQMSLRDALAFSPNTPFVQMIEKVGVKDVVDTSV
KLGLRSYANPGTFNKDNSIADYVKEHNLGSYTLGPTAVNPLELSNVAASLASDGMWCEPSPITTVTDAKGNEVFLDRPAC
EQAIDPEIAQALSAGLSQDTIKGTGAQAAKSLGFSAPTAAKTGTTESHSSAAFLGFNSNFAAASYIYNDGTTVTPLCTSP
VVQCGEGTLFGGDEPARTWFTAAQSSGLAASGNLPSYDPKFNRGVSGSFGTSYVGREGESVRKELSAKGYKVTMTSAPGD
GIPKGSVVNVIAPVPLKPGGTIELFISDGTRPRTTPPSTTRRPNPTEEVVPGTGVSQNDLDDLAHQLQQLLPGF

Sequences:

>Translated_794_residues
MWNSLKDIVLATVAGGVVCALALTPVAAIAGVAVDRTNDTMQSNLQDLTDGSAPGVSTILDNRNNPIAWVYKQRRFEIPS
EAIPQSMKDAIVSIEDRRFYEHKGVDIQGNLRAIVTNIFAGGVAQGASTIDQQYVKNFLLLVKSDNPEEQAAATETSIPR
KLREMRMASTIDSTLSKDDILTRYLNLVHLGNGAFGVEAAARTYFGKGAPELSLAQSAMFAGIVQSSSYLDPYTNPDAVM
DRRNVVLDTMAANGYATPEDVAAAKAEPLGVLEQPQGLPNGCITAGDNGFFCDYVLKYLSEKGLSTDQLTQGSYTINTTL
DPDVQQAAHAAAAGQVNSHTFGVANVMNIVEPGKDSRKILAMTSSRDYGLDLDAGQTVLPQPTSLVGNGAGSVFKIFTAA
VALQQGYGLDTQLQVPPRYEAKGMGDGGAEGCGAGTYCVENSGSYAPQMSLRDALAFSPNTPFVQMIEKVGVKDVVDTSV
KLGLRSYANPGTFNKDNSIADYVKEHNLGSYTLGPTAVNPLELSNVAASLASDGMWCEPSPITTVTDAKGNEVFLDRPAC
EQAIDPEIAQALSAGLSQDTIKGTGAQAAKSLGFSAPTAAKTGTTESHSSAAFLGFNSNFAAASYIYNDGTTVTPLCTSP
VVQCGEGTLFGGDEPARTWFTAAQSSGLAASGNLPSYDPKFNRGVSGSFGTSYVGREGESVRKELSAKGYKVTMTSAPGD
GIPKGSVVNVIAPVPLKPGGTIELFISDGTRPRTTPPSTTRRPNPTEEVVPGTGVSQNDLDDLAHQLQQLLPGF
>Mature_794_residues
MWNSLKDIVLATVAGGVVCALALTPVAAIAGVAVDRTNDTMQSNLQDLTDGSAPGVSTILDNRNNPIAWVYKQRRFEIPS
EAIPQSMKDAIVSIEDRRFYEHKGVDIQGNLRAIVTNIFAGGVAQGASTIDQQYVKNFLLLVKSDNPEEQAAATETSIPR
KLREMRMASTIDSTLSKDDILTRYLNLVHLGNGAFGVEAAARTYFGKGAPELSLAQSAMFAGIVQSSSYLDPYTNPDAVM
DRRNVVLDTMAANGYATPEDVAAAKAEPLGVLEQPQGLPNGCITAGDNGFFCDYVLKYLSEKGLSTDQLTQGSYTINTTL
DPDVQQAAHAAAAGQVNSHTFGVANVMNIVEPGKDSRKILAMTSSRDYGLDLDAGQTVLPQPTSLVGNGAGSVFKIFTAA
VALQQGYGLDTQLQVPPRYEAKGMGDGGAEGCGAGTYCVENSGSYAPQMSLRDALAFSPNTPFVQMIEKVGVKDVVDTSV
KLGLRSYANPGTFNKDNSIADYVKEHNLGSYTLGPTAVNPLELSNVAASLASDGMWCEPSPITTVTDAKGNEVFLDRPAC
EQAIDPEIAQALSAGLSQDTIKGTGAQAAKSLGFSAPTAAKTGTTESHSSAAFLGFNSNFAAASYIYNDGTTVTPLCTSP
VVQCGEGTLFGGDEPARTWFTAAQSSGLAASGNLPSYDPKFNRGVSGSFGTSYVGREGESVRKELSAKGYKVTMTSAPGD
GIPKGSVVNVIAPVPLKPGGTIELFISDGTRPRTTPPSTTRRPNPTEEVVPGTGVSQNDLDDLAHQLQQLLPGF

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=584, Percent_Identity=28.4246575342466, Blast_Score=155, Evalue=9e-39,
Organism=Escherichia coli, GI87082258, Length=358, Percent_Identity=32.6815642458101, Blast_Score=153, Evalue=4e-38,
Organism=Escherichia coli, GI1788867, Length=573, Percent_Identity=25.3054101221641, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789601, Length=162, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 83448; Mature: 83448

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNSLKDIVLATVAGGVVCALALTPVAAIAGVAVDRTNDTMQSNLQDLTDGSAPGVSTIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCHHHHH
DNRNNPIAWVYKQRRFEIPSEAIPQSMKDAIVSIEDRRFYEHKGVDIQGNLRAIVTNIFA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GGVAQGASTIDQQYVKNFLLLVKSDNPEEQAAATETSIPRKLREMRMASTIDSTLSKDDI
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRYLNLVHLGNGAFGVEAAARTYFGKGAPELSLAQSAMFAGIVQSSSYLDPYTNPDAVM
HHHHHHHHEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
DRRNVVLDTMAANGYATPEDVAAAKAEPLGVLEQPQGLPNGCITAGDNGFFCDYVLKYLS
HCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCEEHHHHHHHHH
EKGLSTDQLTQGSYTINTTLDPDVQQAAHAAAAGQVNSHTFGVANVMNIVEPGKDSRKIL
HCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEE
AMTSSRDYGLDLDAGQTVLPQPTSLVGNGAGSVFKIFTAAVALQQGYGLDTQLQVPPRYE
EEECCCCCCCCCCCCCEECCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCC
AKGMGDGGAEGCGAGTYCVENSGSYAPQMSLRDALAFSPNTPFVQMIEKVGVKDVVDTSV
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHH
KLGLRSYANPGTFNKDNSIADYVKEHNLGSYTLGPTAVNPLELSNVAASLASDGMWCEPS
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
PITTVTDAKGNEVFLDRPACEQAIDPEIAQALSAGLSQDTIKGTGAQAAKSLGFSAPTAA
CCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
KTGTTESHSSAAFLGFNSNFAAASYIYNDGTTVTPLCTSPVVQCGEGTLFGGDEPARTWF
CCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCHHHHCCCCCEECCCCHHHHHH
TAAQSSGLAASGNLPSYDPKFNRGVSGSFGTSYVGREGESVRKELSAKGYKVTMTSAPGD
HHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCC
GIPKGSVVNVIAPVPLKPGGTIELFISDGTRPRTTPPSTTRRPNPTEEVVPGTGVSQNDL
CCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCH
DDLAHQLQQLLPGF
HHHHHHHHHHCCCC
>Mature Secondary Structure
MWNSLKDIVLATVAGGVVCALALTPVAAIAGVAVDRTNDTMQSNLQDLTDGSAPGVSTIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCHHHHH
DNRNNPIAWVYKQRRFEIPSEAIPQSMKDAIVSIEDRRFYEHKGVDIQGNLRAIVTNIFA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GGVAQGASTIDQQYVKNFLLLVKSDNPEEQAAATETSIPRKLREMRMASTIDSTLSKDDI
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRYLNLVHLGNGAFGVEAAARTYFGKGAPELSLAQSAMFAGIVQSSSYLDPYTNPDAVM
HHHHHHHHEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
DRRNVVLDTMAANGYATPEDVAAAKAEPLGVLEQPQGLPNGCITAGDNGFFCDYVLKYLS
HCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCEEHHHHHHHHH
EKGLSTDQLTQGSYTINTTLDPDVQQAAHAAAAGQVNSHTFGVANVMNIVEPGKDSRKIL
HCCCCHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEE
AMTSSRDYGLDLDAGQTVLPQPTSLVGNGAGSVFKIFTAAVALQQGYGLDTQLQVPPRYE
EEECCCCCCCCCCCCCEECCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCC
AKGMGDGGAEGCGAGTYCVENSGSYAPQMSLRDALAFSPNTPFVQMIEKVGVKDVVDTSV
CCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHH
KLGLRSYANPGTFNKDNSIADYVKEHNLGSYTLGPTAVNPLELSNVAASLASDGMWCEPS
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
PITTVTDAKGNEVFLDRPACEQAIDPEIAQALSAGLSQDTIKGTGAQAAKSLGFSAPTAA
CCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCC
KTGTTESHSSAAFLGFNSNFAAASYIYNDGTTVTPLCTSPVVQCGEGTLFGGDEPARTWF
CCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCHHHHCCCCCEECCCCHHHHHH
TAAQSSGLAASGNLPSYDPKFNRGVSGSFGTSYVGREGESVRKELSAKGYKVTMTSAPGD
HHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCC
GIPKGSVVNVIAPVPLKPGGTIELFISDGTRPRTTPPSTTRRPNPTEEVVPGTGVSQNDL
CCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCH
DDLAHQLQQLLPGF
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]