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The map label for this gene is mrpD [H]

Identifier: 38232912

GI number: 38232912

Start: 257051

End: 258574

Strand: Reverse

Name: mrpD [H]

Synonym: DIP0291

Alternate gene names: 38232912

Gene position: 258574-257051 (Counterclockwise)

Preceding gene: 38232913

Following gene: 38232911

Centisome position: 10.39

GC content: 58.07

Gene sequence:

>1524_bases
ATGGATTTTGTACTTCCACTGTTTGTGGGCCTCCCACTGCTCACGGTGGCGCTCACTGTCATGGCACCGTGGCGTGTTGT
ACGTGACGGCTTCGCCGTTGTGGTGCCGGTGCTAACGGGTCTAGCTGGTGCGTGGCTGTTTGCTTATACCGCAACGCATG
GCACGATTGCGCACTCGATTGGTTTGTACGTCGGTGGCGTGGCTATCCCCTTTGTGGCGGATCAGTTCAGTGCCATCATG
ATCGTGACTACTTCGATCGTGGCGTTTGCCTCCAACTGGTTTGCGGTGGCTGTGGGTGAGACTCGTGCTCGCTTCTATGC
TCCGCTGTCGCTGATGTTGTTGACCGGTGTGATGGGCGCATTGCTCACCGCTGATCTGTTTAACTTCTTCGTGTTCATTG
AGGTCATGCTGTTGCCTTCTTATGGCTTGATGGCTATGACGGGTACGTGGGCGCGTCTGTCGGCTGGGCGGACGTTCGTA
CTGGTCAACTTGGCTACGTCGACAATTCTGGTCATGGCGGTGTCGGTGCTGTACGGCACTGTGGGTGCTGTCAACATTGC
GGCGCTCGCGGGGGCAGCTCGCGGCAATGGTCCGGTGTCTGTGGTGATGGGCATTGTGGTCATCGCGCTGATTGTCAAAG
CTGGCGTGTTCCCTGTTCACACATGGTTGCCACGTTCCTACCCGGCGACGTCGGCAAGCGTGATGGGCTTGTTCTCTGGC
CTGCACACCAAGGTGGCCGTGTACATGCTGTTCCGCATCTACGTGGTGATTTTCGACCTCGAGCAGCGCTGGATCTGGTT
GATCACCGTGATCTGTATTCTGTCCATGTTGGTCGGCGCTTTTGCCGGTTTGGCTGAAAACTCGATGCGTCGCGTTCTGG
CTTACCAGATGGTCAACGGCATGCCATTCATCTTGGTCATGCTGGCGTTTACCTCCGGCAACGAACAGGCTGCTTTGGCT
GCGGGCATCTTCTACGCCCTGCACCACATGATCACTGTTGGTTCGTTGATTTTGAGTGCCGGCGCTATTGAAGAAACCTA
TGGCACTGACCTGCTCTCCAAGCTCACCGGCATTGCCCGGCGCGACCCGTGGGTTGCTGCTGTGTTCGTGGTCGGCGCGT
TCTCCGTGGTTGGTTTCCCACCATTTTCTGGCCTGTGGGGCAAGGTGTTCATCGTGGTCGGTGTGGCTCATCAGGCTACG
TGGCAGGCCTGGTTGGTAATCGCCGTGATTGTGATTGCAAGCTTTGGTGCGTTCTTGTCCATGCTGCGGTTGTGGCGTGA
GGTGTTCTGGGGTGCGGTGATGAACCGTGAGCGCGTCCCTGAGGCTATGCGGGTGCCGTGGCATCGCTTGGCCCCTGCGG
CGGTGTTGGCGGTCACGTCGTTAGCGATGTTCGTGTTCGCGGGTCCTTTGGTGGGCGCTACTCGTGCGGCTGCTGGCGAC
TTGTTGGATGTGCCGGCCTATACCCAGGCGGTCTTGGGGGATAACCCCATTGGCCTTGCCACTGATCTTCAGGGAGGCAA
ATAA

Upstream 100 bases:

>100_bases
CATTGCAGACCCTCGGCCGCTCGACGACGAATGCGGAAGCCGTGGAGGATCTGGAGCATGCCGCTAAGCGTGCTGCAACC
CGCCAGGAGGAGACTCACTA

Downstream 100 bases:

>100_bases
TTATGCATGCTCCGTTGTATGTTGTGTGGCTGATCGGGCAGATCTTTGTTGCCACTGGTGTGCTGATTAAAGACATGTTC
CATGGGTATAAGAGCATGGA

Product: putative monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MDFVLPLFVGLPLLTVALTVMAPWRVVRDGFAVVVPVLTGLAGAWLFAYTATHGTIAHSIGLYVGGVAIPFVADQFSAIM
IVTTSIVAFASNWFAVAVGETRARFYAPLSLMLLTGVMGALLTADLFNFFVFIEVMLLPSYGLMAMTGTWARLSAGRTFV
LVNLATSTILVMAVSVLYGTVGAVNIAALAGAARGNGPVSVVMGIVVIALIVKAGVFPVHTWLPRSYPATSASVMGLFSG
LHTKVAVYMLFRIYVVIFDLEQRWIWLITVICILSMLVGAFAGLAENSMRRVLAYQMVNGMPFILVMLAFTSGNEQAALA
AGIFYALHHMITVGSLILSAGAIEETYGTDLLSKLTGIARRDPWVAAVFVVGAFSVVGFPPFSGLWGKVFIVVGVAHQAT
WQAWLVIAVIVIASFGAFLSMLRLWREVFWGAVMNRERVPEAMRVPWHRLAPAAVLAVTSLAMFVFAGPLVGATRAAAGD
LLDVPAYTQAVLGDNPIGLATDLQGGK

Sequences:

>Translated_507_residues
MDFVLPLFVGLPLLTVALTVMAPWRVVRDGFAVVVPVLTGLAGAWLFAYTATHGTIAHSIGLYVGGVAIPFVADQFSAIM
IVTTSIVAFASNWFAVAVGETRARFYAPLSLMLLTGVMGALLTADLFNFFVFIEVMLLPSYGLMAMTGTWARLSAGRTFV
LVNLATSTILVMAVSVLYGTVGAVNIAALAGAARGNGPVSVVMGIVVIALIVKAGVFPVHTWLPRSYPATSASVMGLFSG
LHTKVAVYMLFRIYVVIFDLEQRWIWLITVICILSMLVGAFAGLAENSMRRVLAYQMVNGMPFILVMLAFTSGNEQAALA
AGIFYALHHMITVGSLILSAGAIEETYGTDLLSKLTGIARRDPWVAAVFVVGAFSVVGFPPFSGLWGKVFIVVGVAHQAT
WQAWLVIAVIVIASFGAFLSMLRLWREVFWGAVMNRERVPEAMRVPWHRLAPAAVLAVTSLAMFVFAGPLVGATRAAAGD
LLDVPAYTQAVLGDNPIGLATDLQGGK
>Mature_507_residues
MDFVLPLFVGLPLLTVALTVMAPWRVVRDGFAVVVPVLTGLAGAWLFAYTATHGTIAHSIGLYVGGVAIPFVADQFSAIM
IVTTSIVAFASNWFAVAVGETRARFYAPLSLMLLTGVMGALLTADLFNFFVFIEVMLLPSYGLMAMTGTWARLSAGRTFV
LVNLATSTILVMAVSVLYGTVGAVNIAALAGAARGNGPVSVVMGIVVIALIVKAGVFPVHTWLPRSYPATSASVMGLFSG
LHTKVAVYMLFRIYVVIFDLEQRWIWLITVICILSMLVGAFAGLAENSMRRVLAYQMVNGMPFILVMLAFTSGNEQAALA
AGIFYALHHMITVGSLILSAGAIEETYGTDLLSKLTGIARRDPWVAAVFVVGAFSVVGFPPFSGLWGKVFIVVGVAHQAT
WQAWLVIAVIVIASFGAFLSMLRLWREVFWGAVMNRERVPEAMRVPWHRLAPAAVLAVTSLAMFVFAGPLVGATRAAAGD
LLDVPAYTQAVLGDNPIGLATDLQGGK

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=349, Percent_Identity=26.9340974212034, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788613, Length=366, Percent_Identity=25.1366120218579, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693160, Length=429, Percent_Identity=25.6410256410256, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788831, Length=176, Percent_Identity=30.6818181818182, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788829, Length=353, Percent_Identity=24.6458923512748, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367154, Length=285, Percent_Identity=24.5614035087719, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54028; Mature: 54028

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFVLPLFVGLPLLTVALTVMAPWRVVRDGFAVVVPVLTGLAGAWLFAYTATHGTIAHSI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLYVGGVAIPFVADQFSAIMIVTTSIVAFASNWFAVAVGETRARFYAPLSLMLLTGVMGA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LLTADLFNFFVFIEVMLLPSYGLMAMTGTWARLSAGRTFVLVNLATSTILVMAVSVLYGT
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHH
VGAVNIAALAGAARGNGPVSVVMGIVVIALIVKAGVFPVHTWLPRSYPATSASVMGLFSG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHH
LHTKVAVYMLFRIYVVIFDLEQRWIWLITVICILSMLVGAFAGLAENSMRRVLAYQMVNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPFILVMLAFTSGNEQAALAAGIFYALHHMITVGSLILSAGAIEETYGTDLLSKLTGIAR
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
RDPWVAAVFVVGAFSVVGFPPFSGLWGKVFIVVGVAHQATWQAWLVIAVIVIASFGAFLS
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MLRLWREVFWGAVMNRERVPEAMRVPWHRLAPAAVLAVTSLAMFVFAGPLVGATRAAAGD
HHHHHHHHHHHHHHCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LLDVPAYTQAVLGDNPIGLATDLQGGK
CCCCCHHHHHHHCCCCCCEECCCCCCC
>Mature Secondary Structure
MDFVLPLFVGLPLLTVALTVMAPWRVVRDGFAVVVPVLTGLAGAWLFAYTATHGTIAHSI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLYVGGVAIPFVADQFSAIMIVTTSIVAFASNWFAVAVGETRARFYAPLSLMLLTGVMGA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LLTADLFNFFVFIEVMLLPSYGLMAMTGTWARLSAGRTFVLVNLATSTILVMAVSVLYGT
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHH
VGAVNIAALAGAARGNGPVSVVMGIVVIALIVKAGVFPVHTWLPRSYPATSASVMGLFSG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHH
LHTKVAVYMLFRIYVVIFDLEQRWIWLITVICILSMLVGAFAGLAENSMRRVLAYQMVNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPFILVMLAFTSGNEQAALAAGIFYALHHMITVGSLILSAGAIEETYGTDLLSKLTGIAR
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
RDPWVAAVFVVGAFSVVGFPPFSGLWGKVFIVVGVAHQATWQAWLVIAVIVIASFGAFLS
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
MLRLWREVFWGAVMNRERVPEAMRVPWHRLAPAAVLAVTSLAMFVFAGPLVGATRAAAGD
HHHHHHHHHHHHHHCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LLDVPAYTQAVLGDNPIGLATDLQGGK
CCCCCHHHHHHHCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]