Click here to switch to the map view.

The map label for this gene is phrA [H]

Identifier: 38232742

GI number: 38232742

Start: 93040

End: 94380

Strand: Direct

Name: phrA [H]

Synonym: DIP0113

Alternate gene names: 38232742

Gene position: 93040-94380 (Clockwise)

Preceding gene: 38232741

Following gene: 38232743

Centisome position: 3.74

GC content: 61.97

Gene sequence:

>1341_bases
ATGGCTCGCTCAGTGGTGGTGTGGTTCCGTGACGATTTACGCGTCCACGATAATCCAGCTCTTATGAAGGCATGGGAGCT
TGTTCGTGCCAACCCTGCGGACTTGCATGCGGTCTACATTGCCAACGAGGTGGGGGTTCGCCCCCTTGGTGGGGCAGTCA
AGTGGTGGCTGCACCACAGCCTGCTGGCATTGTCTGAGCAGCTGGCGCAGCGTGGTGTGCGTCTGCATGTGCTCTCCGGT
GACCCACTCACGCTGTTGCCACAGCTAGTGACTTCCTGTGGTGCTACAGCGGTGACGATGAATCGTCGCTATGATCCCGC
AGCACGCAGTATTGATGATGCATTCGTCGCTGATGCCAGTGCCCACGATGTGGAGGTCTACGACTTCCCTTGCCATCTGC
TGGCAGAACCAGGGGAGATCACCACCACAACCGGTGGCAGCTACAAGGTGTTTACGCCCTTTTCCCGTAACCTTCGCGAC
GCCATCGGTGACCTGCCCTTAGATACGCTTGCGGCACCACCCAAGGCCGAACAGCCCATAGACGACACGGAAACCCAGGC
CGCGATTGCGGACTTAGGCTGGGACGCATGGTGGGCTGCGTCGATAAGCAAGGCGTGGACCCCAGGTGAACCCGCCGCCC
GCGAAGCCCTCGCCGAGCTCGACGACATCCTCCCGCGCTACCTAGACGACCGCGACCGCCCTGACATCGACGGCACTTCT
AGGCTAAGCCCGCGCCTGCGCTTCGGGGAACTCAGCGTCGCTGAGGTGTGGAACCATGCCCACACCTCGGAGGGGTTCCG
CCGCCAACTCATGTGGCGAGATTTCGCCTGGCACCGCCTCGACGCGCACCCCGACATGGCGACCGCCAACATCCGCCCCG
AATTTGACCACTTCCCTTGGGACGGCGGTGACTTCGAAGCCGAACTGAACGCTTGGCGTCATGGCCGCACCGGCATCGCG
CTTGTCGACGCCGGCATGCGCGAACTATGGGCCACCGGAACCATGCACAACCGTGTGCGCATGGTCGCCGCATCCCTGCT
GGTCAAAAACCTAGGAATCCACTGGCGGCACGGCGAGCAATGGTTCTGGGACACCCTCGTTGATGCCGACCCAGCGTCCA
ACCCCTTCAACTGGCAATGGGTAGCCGGCAGTGGTGACGACGCCGCCCCCTACTTCCGCATCTTCAACCCCGATACCCAA
GCGCGCCGCTTTGACCCCGACGGCACCTACCGCACGCGATGGCTGCCCATCATGAGCGCGGACTATCCCGAAGAGGCGAT
CGTAGACCTTAAAGAATCCCGACTTCGGGCTCTCGACGCCTATAACGCTTGTAAACGCTGA

Upstream 100 bases:

>100_bases
AGCCGATTCCTTTCGATCTGCTGCGCGATGTGATCGCCTTTAACATCGACGATAAACGCGATGTGACAAGTTTCTGGCGC
AAGTAATACAGGAGGATATG

Downstream 100 bases:

>100_bases
GTAATCAGATAGGTTAAGGGGTATGAGAAAACTGTATTATGCCTCGTTAACCTACCTCATTCTCGGGCTCGTAGCCGGGG
TGTTCTACCGTGAATGGACC

Product: putative riboflavin biosynthesis protein

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MARSVVVWFRDDLRVHDNPALMKAWELVRANPADLHAVYIANEVGVRPLGGAVKWWLHHSLLALSEQLAQRGVRLHVLSG
DPLTLLPQLVTSCGATAVTMNRRYDPAARSIDDAFVADASAHDVEVYDFPCHLLAEPGEITTTTGGSYKVFTPFSRNLRD
AIGDLPLDTLAAPPKAEQPIDDTETQAAIADLGWDAWWAASISKAWTPGEPAAREALAELDDILPRYLDDRDRPDIDGTS
RLSPRLRFGELSVAEVWNHAHTSEGFRRQLMWRDFAWHRLDAHPDMATANIRPEFDHFPWDGGDFEAELNAWRHGRTGIA
LVDAGMRELWATGTMHNRVRMVAASLLVKNLGIHWRHGEQWFWDTLVDADPASNPFNWQWVAGSGDDAAPYFRIFNPDTQ
ARRFDPDGTYRTRWLPIMSADYPEEAIVDLKESRLRALDAYNACKR

Sequences:

>Translated_446_residues
MARSVVVWFRDDLRVHDNPALMKAWELVRANPADLHAVYIANEVGVRPLGGAVKWWLHHSLLALSEQLAQRGVRLHVLSG
DPLTLLPQLVTSCGATAVTMNRRYDPAARSIDDAFVADASAHDVEVYDFPCHLLAEPGEITTTTGGSYKVFTPFSRNLRD
AIGDLPLDTLAAPPKAEQPIDDTETQAAIADLGWDAWWAASISKAWTPGEPAAREALAELDDILPRYLDDRDRPDIDGTS
RLSPRLRFGELSVAEVWNHAHTSEGFRRQLMWRDFAWHRLDAHPDMATANIRPEFDHFPWDGGDFEAELNAWRHGRTGIA
LVDAGMRELWATGTMHNRVRMVAASLLVKNLGIHWRHGEQWFWDTLVDADPASNPFNWQWVAGSGDDAAPYFRIFNPDTQ
ARRFDPDGTYRTRWLPIMSADYPEEAIVDLKESRLRALDAYNACKR
>Mature_445_residues
ARSVVVWFRDDLRVHDNPALMKAWELVRANPADLHAVYIANEVGVRPLGGAVKWWLHHSLLALSEQLAQRGVRLHVLSGD
PLTLLPQLVTSCGATAVTMNRRYDPAARSIDDAFVADASAHDVEVYDFPCHLLAEPGEITTTTGGSYKVFTPFSRNLRDA
IGDLPLDTLAAPPKAEQPIDDTETQAAIADLGWDAWWAASISKAWTPGEPAAREALAELDDILPRYLDDRDRPDIDGTSR
LSPRLRFGELSVAEVWNHAHTSEGFRRQLMWRDFAWHRLDAHPDMATANIRPEFDHFPWDGGDFEAELNAWRHGRTGIAL
VDAGMRELWATGTMHNRVRMVAASLLVKNLGIHWRHGEQWFWDTLVDADPASNPFNWQWVAGSGDDAAPYFRIFNPDTQA
RRFDPDGTYRTRWLPIMSADYPEEAIVDLKESRLRALDAYNACKR

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=467, Percent_Identity=27.6231263383298, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI188536100, Length=456, Percent_Identity=28.0701754385965, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI188536103, Length=410, Percent_Identity=27.0731707317073, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786926, Length=488, Percent_Identity=32.9918032786885, Blast_Score=202, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324962, Length=503, Percent_Identity=30.2186878727634, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17137248, Length=467, Percent_Identity=27.8372591006424, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585455, Length=467, Percent_Identity=27.8372591006424, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648152, Length=475, Percent_Identity=26.3157894736842, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 50284; Mature: 50153

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSVVVWFRDDLRVHDNPALMKAWELVRANPADLHAVYIANEVGVRPLGGAVKWWLHHS
CCCEEEEEEECCCEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
LLALSEQLAQRGVRLHVLSGDPLTLLPQLVTSCGATAVTMNRRYDPAARSIDDAFVADAS
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHCCCC
AHDVEVYDFPCHLLAEPGEITTTTGGSYKVFTPFSRNLRDAIGDLPLDTLAAPPKAEQPI
CCCEEEEECCHHEECCCCCEEEECCCCEEEECCHHHHHHHHHCCCCHHHHCCCCCCCCCC
DDTETQAAIADLGWDAWWAASISKAWTPGEPAAREALAELDDILPRYLDDRDRPDIDGTS
CCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RLSPRLRFGELSVAEVWNHAHTSEGFRRQLMWRDFAWHRLDAHPDMATANIRPEFDHFPW
CCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCC
DGGDFEAELNAWRHGRTGIALVDAGMRELWATGTMHNRVRMVAASLLVKNLGIHWRHGEQ
CCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCC
WFWDTLVDADPASNPFNWQWVAGSGDDAAPYFRIFNPDTQARRFDPDGTYRTRWLPIMSA
HHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECC
DYPEEAIVDLKESRLRALDAYNACKR
CCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARSVVVWFRDDLRVHDNPALMKAWELVRANPADLHAVYIANEVGVRPLGGAVKWWLHHS
CCEEEEEEECCCEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
LLALSEQLAQRGVRLHVLSGDPLTLLPQLVTSCGATAVTMNRRYDPAARSIDDAFVADAS
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCHHHHCCCHHHHCCCC
AHDVEVYDFPCHLLAEPGEITTTTGGSYKVFTPFSRNLRDAIGDLPLDTLAAPPKAEQPI
CCCEEEEECCHHEECCCCCEEEECCCCEEEECCHHHHHHHHHCCCCHHHHCCCCCCCCCC
DDTETQAAIADLGWDAWWAASISKAWTPGEPAAREALAELDDILPRYLDDRDRPDIDGTS
CCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RLSPRLRFGELSVAEVWNHAHTSEGFRRQLMWRDFAWHRLDAHPDMATANIRPEFDHFPW
CCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCC
DGGDFEAELNAWRHGRTGIALVDAGMRELWATGTMHNRVRMVAASLLVKNLGIHWRHGEQ
CCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCC
WFWDTLVDADPASNPFNWQWVAGSGDDAAPYFRIFNPDTQARRFDPDGTYRTRWLPIMSA
HHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECC
DYPEEAIVDLKESRLRALDAYNACKR
CCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]