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The map label for this gene is dapE [H]

Identifier: 38232734

GI number: 38232734

Start: 84975

End: 86321

Strand: Reverse

Name: dapE [H]

Synonym: DIP0104

Alternate gene names: 38232734

Gene position: 86321-84975 (Counterclockwise)

Preceding gene: 38232744

Following gene: 38232733

Centisome position: 3.47

GC content: 62.66

Gene sequence:

>1347_bases
ATGTCAATCACCCCCATTGCCCTTAATGTGGTACACATGACGCTTTACGACGACACCCTCGCCCTGCTCACCGAACTCAT
CCGCAACGCCTGCGTCAACGACCTCACACCAGACTCCGGCCACGAGGAGCGCAATGCGGCCACTTTAGAAGAGTTCTTCG
CAGGAAGTCCCGTGAACATCCAACGCTTCGAACCTCACCCAGGGCGCGTCAGTCTCGTTGTCACCGTCGCCGGAAGCGAC
CTCGCCGCCGAACCCCTCACCTTCCTAGGCCACACCGATGTGGTGCCCGTCGATACGCAACATTGGAGCGTCCCGCCTTT
CGACGCCACCATCATCGATGGCAAACTCTACGGCCGCGGCAGCGTCGACATGCTCTTTATTACCGCCACCATGGCCGCCG
TCACCCGCCACGTTGCCCGCACCGGTGGCAACGCCGGCACCCTTTACTTCACAGCGCTTGCCGACGAAGAAGCCCGCGGT
GGCCTCGGCGCAGCATGGCTAGCCCAACACCACCCCGACGCGTTGAGCTGGAAGAACTGCATCTCTGAAACCGGCGGGTC
CCACATCCCAGGCCGCGATGACTCCGACTCCACCATCATCTACGTCGGTGAAAAAGGTGCTGCCCAGCGTCGCCTCCACG
TCTATGGCGACGCCGGCCACGGCTCCGCCCCCTATAACAAGGACAGCGCCATCGCCACGATCGGCGAAGTAGCACGCCGG
ATCGCCGCCTTTGAACCCGAGGTAAGCACCGACGACATCTGGCACGGCTTTATCGACGCCTTCCGCTTCGACCCCACCAC
TACCGCCGCCCTGCGAGACGGCACCGGCTACGAACACCTCGGCGACCTCGCAGCCTTCGGCCACGCCATCTCCCACCTCA
CCATCGCCCAAACCGTGCTGCGCGCAGGTCAAGCCATCAACGTCCTTCCCAGCCACGCCTGGCTCGACATGGACATCCGT
ACTCTCCCAGGGCACGACGACGCCTACGTGGACGACCTGCTCACCCAAGTCCTCGGTGATCTCGCACCCCACGTCACCAT
CGAACACCTCATCTGCGAACCCGCCACCATCTCCCCCACCGACACCCCGCTCTACCGCACACTAGCCGAGGTCCTCCACG
ACTCTTTCCCCGACACCACCGTGATCCCCATGATCGCACCCGGTGGTTCTGACCTGCGCTTTGCACGGCGAATGGGTGGC
AATGGCTACGGATTTGCAGTCCATGCACCCGACCGCACCCTAGGGCATGTTCACGGCCAGCTCCACTCCCATGACGAGTA
CCTCCACCTCGAAGATCTCCGCCTCACTGTTGCCGGATACCAGCAGCTAGTAGAGAAATTTCTGTAG

Upstream 100 bases:

>100_bases
GCGAGTTCTAGAATGTCAGTCACGTGATGCTCCCATGTTGTGGCAATTGGTAGATGTCCAAAAATCCTAGCGATAATCAT
TGAACGGTGTTTAATCTTGG

Downstream 100 bases:

>100_bases
ACTGACCTGCATGGATATTCTCAACAAAGCTAAAGACCTGATCAACAACAACTCCGACAAGGTGAAGGAGGCCGTGGATA
AGGCCGGCGACTTTATCGAC

Product: putative peptidase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSITPIALNVVHMTLYDDTLALLTELIRNACVNDLTPDSGHEERNAATLEEFFAGSPVNIQRFEPHPGRVSLVVTVAGSD
LAAEPLTFLGHTDVVPVDTQHWSVPPFDATIIDGKLYGRGSVDMLFITATMAAVTRHVARTGGNAGTLYFTALADEEARG
GLGAAWLAQHHPDALSWKNCISETGGSHIPGRDDSDSTIIYVGEKGAAQRRLHVYGDAGHGSAPYNKDSAIATIGEVARR
IAAFEPEVSTDDIWHGFIDAFRFDPTTTAALRDGTGYEHLGDLAAFGHAISHLTIAQTVLRAGQAINVLPSHAWLDMDIR
TLPGHDDAYVDDLLTQVLGDLAPHVTIEHLICEPATISPTDTPLYRTLAEVLHDSFPDTTVIPMIAPGGSDLRFARRMGG
NGYGFAVHAPDRTLGHVHGQLHSHDEYLHLEDLRLTVAGYQQLVEKFL

Sequences:

>Translated_448_residues
MSITPIALNVVHMTLYDDTLALLTELIRNACVNDLTPDSGHEERNAATLEEFFAGSPVNIQRFEPHPGRVSLVVTVAGSD
LAAEPLTFLGHTDVVPVDTQHWSVPPFDATIIDGKLYGRGSVDMLFITATMAAVTRHVARTGGNAGTLYFTALADEEARG
GLGAAWLAQHHPDALSWKNCISETGGSHIPGRDDSDSTIIYVGEKGAAQRRLHVYGDAGHGSAPYNKDSAIATIGEVARR
IAAFEPEVSTDDIWHGFIDAFRFDPTTTAALRDGTGYEHLGDLAAFGHAISHLTIAQTVLRAGQAINVLPSHAWLDMDIR
TLPGHDDAYVDDLLTQVLGDLAPHVTIEHLICEPATISPTDTPLYRTLAEVLHDSFPDTTVIPMIAPGGSDLRFARRMGG
NGYGFAVHAPDRTLGHVHGQLHSHDEYLHLEDLRLTVAGYQQLVEKFL
>Mature_447_residues
SITPIALNVVHMTLYDDTLALLTELIRNACVNDLTPDSGHEERNAATLEEFFAGSPVNIQRFEPHPGRVSLVVTVAGSDL
AAEPLTFLGHTDVVPVDTQHWSVPPFDATIIDGKLYGRGSVDMLFITATMAAVTRHVARTGGNAGTLYFTALADEEARGG
LGAAWLAQHHPDALSWKNCISETGGSHIPGRDDSDSTIIYVGEKGAAQRRLHVYGDAGHGSAPYNKDSAIATIGEVARRI
AAFEPEVSTDDIWHGFIDAFRFDPTTTAALRDGTGYEHLGDLAAFGHAISHLTIAQTVLRAGQAINVLPSHAWLDMDIRT
LPGHDDAYVDDLLTQVLGDLAPHVTIEHLICEPATISPTDTPLYRTLAEVLHDSFPDTTVIPMIAPGGSDLRFARRMGGN
GYGFAVHAPDRTLGHVHGQLHSHDEYLHLEDLRLTVAGYQQLVEKFL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=375, Percent_Identity=24.8, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788816, Length=369, Percent_Identity=24.9322493224932, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24649206, Length=384, Percent_Identity=22.1354166666667, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 48414; Mature: 48283

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITPIALNVVHMTLYDDTLALLTELIRNACVNDLTPDSGHEERNAATLEEFFAGSPVNI
CCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
QRFEPHPGRVSLVVTVAGSDLAAEPLTFLGHTDVVPVDTQHWSVPPFDATIIDGKLYGRG
EEECCCCCEEEEEEEECCCCCCCCCHHHCCCCCEEECCCCCCCCCCCCCEEECCEEECCC
SVDMLFITATMAAVTRHVARTGGNAGTLYFTALADEEARGGLGAAWLAQHHPDALSWKNC
CCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHH
ISETGGSHIPGRDDSDSTIIYVGEKGAAQRRLHVYGDAGHGSAPYNKDSAIATIGEVARR
HHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IAAFEPEVSTDDIWHGFIDAFRFDPTTTAALRDGTGYEHLGDLAAFGHAISHLTIAQTVL
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
RAGQAINVLPSHAWLDMDIRTLPGHDDAYVDDLLTQVLGDLAPHVTIEHLICEPATISPT
HCCCEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCC
DTPLYRTLAEVLHDSFPDTTVIPMIAPGGSDLRFARRMGGNGYGFAVHAPDRTLGHVHGQ
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHCC
LHSHDEYLHLEDLRLTVAGYQQLVEKFL
CCCCCCEEEHHHHEEEHHHHHHHHHHHC
>Mature Secondary Structure 
SITPIALNVVHMTLYDDTLALLTELIRNACVNDLTPDSGHEERNAATLEEFFAGSPVNI
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
QRFEPHPGRVSLVVTVAGSDLAAEPLTFLGHTDVVPVDTQHWSVPPFDATIIDGKLYGRG
EEECCCCCEEEEEEEECCCCCCCCCHHHCCCCCEEECCCCCCCCCCCCCEEECCEEECCC
SVDMLFITATMAAVTRHVARTGGNAGTLYFTALADEEARGGLGAAWLAQHHPDALSWKNC
CCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHH
ISETGGSHIPGRDDSDSTIIYVGEKGAAQRRLHVYGDAGHGSAPYNKDSAIATIGEVARR
HHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IAAFEPEVSTDDIWHGFIDAFRFDPTTTAALRDGTGYEHLGDLAAFGHAISHLTIAQTVL
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
RAGQAINVLPSHAWLDMDIRTLPGHDDAYVDDLLTQVLGDLAPHVTIEHLICEPATISPT
HCCCEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCC
DTPLYRTLAEVLHDSFPDTTVIPMIAPGGSDLRFARRMGGNGYGFAVHAPDRTLGHVHGQ
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHCC
LHSHDEYLHLEDLRLTVAGYQQLVEKFL
CCCCCCEEEHHHHEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA