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The map label for this gene is thiO [H]

Identifier: 38232673

GI number: 38232673

Start: 30015

End: 31103

Strand: Direct

Name: thiO [H]

Synonym: DIP0031

Alternate gene names: 38232673

Gene position: 30015-31103 (Clockwise)

Preceding gene: 38232672

Following gene: 38232674

Centisome position: 1.21

GC content: 58.77

Gene sequence:

>1089_bases
ATGAAAATAGCCGTTGTAGGTGGTGGAATCGTAGGGCTAAGCACCGCCTTTGAACTTAGTTCCCGCGGGCATAGTGTTCA
CGTATTTGACCCCAACCCAGCAAGTGGGGCCTCACACTTTGCCGGCGGAATGCTCGCCCCCGCAGCCGAAGTGCAGTTCC
AACAAGACCCGTTGTTTCCGTTGATGAAGCGCGCCGGTAAGCTGTGGCCAGACATGGTGCGTCGGGTAGCGCAGCGCACC
AACCTGCCTACCGGTTATCGCACCGAAGGCACCCTCGTTGTGGCAGCCGATCGCGCCGACGCGGAACACCTTAAACAATT
GCGCGCAACCCAAGAGGCGGCCGGAATGGATGTTCGCCCCATTACTACCCGACAGGCCCGTGGCCTCGAACCGGCACTAG
GACCTCGGCTATCGGCGGCCGTACACATCCCCAACGACACCCAAGTCGCACCACGCGTATTCCTCACCGCACTACTCGAC
GCACTCGATGACTGTGGAGTAGGGGTAATAAAAGAGAAAATCACCGACCTCGAACCCCTCTACCAACAATTCGATGTTGT
TGTCCTCGCAGCAGGCCTTGGTGCACAACACCTAAGCCCCATACCGTTAGCACTACGACCCGTGCGCGGCGACATCCTAC
GAGTACAAACAGAACCAGGGGCAGTCAACATGGTTGTGCGCGGCTGGGTCAACGACCGCCCCATCTACATCATCCCGCGC
GCAAACGGCGAAATCGCCATCGGAGCCACCAGCCGCGAAGACGAACGCGATTTGCCCAGTGTAGAAGGAATATACGACCT
GCTCCGCGATGCCATACGCGTAGTACCGGGTATCGTCGATAGTTCCCTTATAGAAGCCAACGTTGGTGTACGCCCCGGCA
CACCCGACGACCTCCCATACCTCGGGTGGGCATCAGACCGACTCATTATTTCCACCGGATACTTCCGCCACGGCATCTTG
CTCTCAGCACTCGGCGCCCACGTCACCGCATGCCTCATCGACGGCACCGACCCCGGCATCGACCTCACAGCATGCGCACC
AGATCGACACCACAACGAAAGGGGCACAACCCATGGACATCTACATTAA

Upstream 100 bases:

>100_bases
TTGCTGCAACGGGGGTCGCTGGTGTGGCAATTGTGCGCGCATTAATGAACTCACAGGGCGTAGCAACCGATGTAAAACTC
GTATTGAAAGGTTTTGCACA

Downstream 100 bases:

>100_bases
CGACATCCCCACCGCCATAGAATCACCCCAGCTCACCGACATTATCAGCAACCACTGCAACGGAATCCGCCGAGGGATTG
CCGTAGCCATCAACCAACGC

Product: putative thiamine biosynthesis oxidoreductase

Products: NA

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKIAVVGGGIVGLSTAFELSSRGHSVHVFDPNPASGASHFAGGMLAPAAEVQFQQDPLFPLMKRAGKLWPDMVRRVAQRT
NLPTGYRTEGTLVVAADRADAEHLKQLRATQEAAGMDVRPITTRQARGLEPALGPRLSAAVHIPNDTQVAPRVFLTALLD
ALDDCGVGVIKEKITDLEPLYQQFDVVVLAAGLGAQHLSPIPLALRPVRGDILRVQTEPGAVNMVVRGWVNDRPIYIIPR
ANGEIAIGATSREDERDLPSVEGIYDLLRDAIRVVPGIVDSSLIEANVGVRPGTPDDLPYLGWASDRLIISTGYFRHGIL
LSALGAHVTACLIDGTDPGIDLTACAPDRHHNERGTTHGHLH

Sequences:

>Translated_362_residues
MKIAVVGGGIVGLSTAFELSSRGHSVHVFDPNPASGASHFAGGMLAPAAEVQFQQDPLFPLMKRAGKLWPDMVRRVAQRT
NLPTGYRTEGTLVVAADRADAEHLKQLRATQEAAGMDVRPITTRQARGLEPALGPRLSAAVHIPNDTQVAPRVFLTALLD
ALDDCGVGVIKEKITDLEPLYQQFDVVVLAAGLGAQHLSPIPLALRPVRGDILRVQTEPGAVNMVVRGWVNDRPIYIIPR
ANGEIAIGATSREDERDLPSVEGIYDLLRDAIRVVPGIVDSSLIEANVGVRPGTPDDLPYLGWASDRLIISTGYFRHGIL
LSALGAHVTACLIDGTDPGIDLTACAPDRHHNERGTTHGHLH
>Mature_362_residues
MKIAVVGGGIVGLSTAFELSSRGHSVHVFDPNPASGASHFAGGMLAPAAEVQFQQDPLFPLMKRAGKLWPDMVRRVAQRT
NLPTGYRTEGTLVVAADRADAEHLKQLRATQEAAGMDVRPITTRQARGLEPALGPRLSAAVHIPNDTQVAPRVFLTALLD
ALDDCGVGVIKEKITDLEPLYQQFDVVVLAAGLGAQHLSPIPLALRPVRGDILRVQTEPGAVNMVVRGWVNDRPIYIIPR
ANGEIAIGATSREDERDLPSVEGIYDLLRDAIRVVPGIVDSSLIEANVGVRPGTPDDLPYLGWASDRLIISTGYFRHGIL
LSALGAHVTACLIDGTDPGIDLTACAPDRHHNERGTTHGHLH

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 38790; Mature: 38790

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVVGGGIVGLSTAFELSSRGHSVHVFDPNPASGASHFAGGMLAPAAEVQFQQDPLFP
CEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHCEECCCCCHH
LMKRAGKLWPDMVRRVAQRTNLPTGYRTEGTLVVAADRADAEHLKQLRATQEAAGMDVRP
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
ITTRQARGLEPALGPRLSAAVHIPNDTQVAPRVFLTALLDALDDCGVGVIKEKITDLEPL
CCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YQQFDVVVLAAGLGAQHLSPIPLALRPVRGDILRVQTEPGAVNMVVRGWVNDRPIYIIPR
HHHHCEEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEECCCCCCEEEEEC
ANGEIAIGATSREDERDLPSVEGIYDLLRDAIRVVPGIVDSSLIEANVGVRPGTPDDLPY
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHCCCCCCCCCCCCCCCC
LGWASDRLIISTGYFRHGILLSALGAHVTACLIDGTDPGIDLTACAPDRHHNERGTTHGH
CCCCCCEEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCC
LH
CC
>Mature Secondary Structure
MKIAVVGGGIVGLSTAFELSSRGHSVHVFDPNPASGASHFAGGMLAPAAEVQFQQDPLFP
CEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHCEECCCCCHH
LMKRAGKLWPDMVRRVAQRTNLPTGYRTEGTLVVAADRADAEHLKQLRATQEAAGMDVRP
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
ITTRQARGLEPALGPRLSAAVHIPNDTQVAPRVFLTALLDALDDCGVGVIKEKITDLEPL
CCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YQQFDVVVLAAGLGAQHLSPIPLALRPVRGDILRVQTEPGAVNMVVRGWVNDRPIYIIPR
HHHHCEEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEEEEECCCCCCEEEEEC
ANGEIAIGATSREDERDLPSVEGIYDLLRDAIRVVPGIVDSSLIEANVGVRPGTPDDLPY
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHCCCCCCCCCCCCCCCC
LGWASDRLIISTGYFRHGILLSALGAHVTACLIDGTDPGIDLTACAPDRHHNERGTTHGH
CCCCCCEEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCC
LH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA