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The map label for this gene is purD [H]

Identifier: 37681327

GI number: 37681327

Start: 3227197

End: 3228531

Strand: Direct

Name: purD [H]

Synonym: VV3143

Alternate gene names: 37681327

Gene position: 3227197-3228531 (Clockwise)

Preceding gene: 37681326

Following gene: 37681330

Centisome position: 96.2

GC content: 53.26

Gene sequence:

>1335_bases
GTGCCTTATCAGGCACCTCTTTTTGCAAGATTTGAGGATGATGAGATGAATGTATTGATAATTGGTTCCGGCGGTCGTGA
ACACGCTCTAGGTTGGAAAGCCGCGCAAAACCCCAATGTGGAGACAATCTTCGTCGCGCCGGGTAACGCTGGTACCGCAC
TTGAGCCTAAGCTAGAAAACGTCAACATCGCCGTTGAAGACATTGCCGGTTTAGTGGCGTTTGCTAAAGAGAAAGCCATT
GAACTGACCATCGTCGGTCCTGAAGTGCCACTCGTACTGGGTGTCGTTGATGCGTTTTATGAAGCAGGCCTGCCAATTTT
TGGCCCTACCCAAGCGGCTGCGCAACTTGAAGGCTCTAAAGCATTTACCAAAGACTTCCTTGCTCGTCACCAAATCCCGA
CGGCGGCGTACGCAAACTTTACGGACATCGAGCCAGCACTGGCCTATGTACGTGAACAAGGCGCGCCAATCGTGGTGAAA
GCAGATGGCCTTGCGGCAGGTAAAGGCGTGATTGTCGCGATGACGCTGGAAGAAGCGGAAGAAGCAATCAAAGACATGCT
CGCAGGCAACGCCTTTGGCGAGGCAGGCAGCCGCGTGGTGATCGAAGAGTTCCTTGATGGCGAAGAAGCGAGCTTCATCG
TGATGGTGGATGGCGAAAACGTACTACCGATGGCCACCAGCCAAGACCACAAACGTGTCGGCGATAAAGACACCGGTCCA
AACACCGGTGGCATGGGCGCTTACTCACCAGCACCAGTGGTAACACCTGAGATCCATAACCGCGTTATGCAAGAGGTGAT
CTTCCCTACCGTGCGTGGCATGGCAGCCGAAGGTAACCCATACACGGGATTCCTCTATGCCGGTTTGATGATTGACAAAG
ATGGCACACCAAAAGTGATCGAGTACAACTGCCGCTTTGGTGACCCAGAAACGCAACCTATCATGATGCGCATGGAATCG
GATCTTGTGGAATTGTGCCTCGCGGCCATCGACAAGAAACTGGACCAAGTCGAGTCCAAGTGGGACCCTCGCGCATCGAT
TGGTATTGTGCTTGCAGCGGGTGGCTATCCAGCGGCGTACAACAAGGGTGACGTGATCTCTGGCCTACCTCAAGTTGAAA
TTGAAGGCGAAAAAGTCTTCCACGCTGGCACTGAAAATAAAGGGGGCGATATCGTCACAAACGGTGGTCGTGTTCTTTGT
GCCACCGCACTGGGCAACAGCGTGTCAGAAGCGCAGCAACGCGCGTACGAGCTGGCAAAACAGATCCGTTGGGATGGGAT
GTTCCACCGCAACGATATTGGCTACCGTGCTATCGCTCGCGAGCAACAAAAATAA

Upstream 100 bases:

>100_bases
TTCGATGCGTGATGACGAAGTGATTGCCGCCGCAGATGAACACGGTATGGCGATGATCTTTACCGGCATGCGCCACTTCC
GCCACTAGTTTTTATCAGAG

Downstream 100 bases:

>100_bases
CAACAACATCAGTTGGCAAAGAAAACAAAAGGCGCATTTTGCGCCTTTTGTCTTTTCATTCGTCTAACGGAGTAAGTCTT
ACTCTTCAAGTAACACGGGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPYQAPLFARFEDDEMNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFAKEKAI
ELTIVGPEVPLVLGVVDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVK
ADGLAAGKGVIVAMTLEEAEEAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGP
NTGGMGAYSPAPVVTPEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIMMRMES
DLVELCLAAIDKKLDQVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIEGEKVFHAGTENKGGDIVTNGGRVLC
ATALGNSVSEAQQRAYELAKQIRWDGMFHRNDIGYRAIAREQQK

Sequences:

>Translated_444_residues
MPYQAPLFARFEDDEMNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFAKEKAI
ELTIVGPEVPLVLGVVDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVK
ADGLAAGKGVIVAMTLEEAEEAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGP
NTGGMGAYSPAPVVTPEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIMMRMES
DLVELCLAAIDKKLDQVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIEGEKVFHAGTENKGGDIVTNGGRVLC
ATALGNSVSEAQQRAYELAKQIRWDGMFHRNDIGYRAIAREQQK
>Mature_443_residues
PYQAPLFARFEDDEMNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFAKEKAIE
LTIVGPEVPLVLGVVDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVKA
DGLAAGKGVIVAMTLEEAEEAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGPN
TGGMGAYSPAPVVTPEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIMMRMESD
LVELCLAAIDKKLDQVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIEGEKVFHAGTENKGGDIVTNGGRVLCA
TALGNSVSEAQQRAYELAKQIRWDGMFHRNDIGYRAIAREQQK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=49.4172494172494, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=49.4172494172494, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=49.4172494172494, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=49.4172494172494, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=75.2913752913753, Blast_Score=667, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=41.8823529411765, Blast_Score=316, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.6966292134831, Blast_Score=359, Evalue=1e-100,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.5813953488372, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.5813953488372, Blast_Score=351, Evalue=5e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 47556; Mature: 47424

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYQAPLFARFEDDEMNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLEN
CCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
VNIAVEDIAGLVAFAKEKAIELTIVGPEVPLVLGVVDAFYEAGLPIFGPTQAAAQLEGSK
CCEEHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCH
AFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAE
HHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHH
EAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGP
HHHHHHHCCCCCCCCCCEEEHHHHCCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCC
NTGGMGAYSPAPVVTPEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCEE
EYNCRFGDPETQPIMMRMESDLVELCLAAIDKKLDQVESKWDPRASIGIVLAAGGYPAAY
EEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
NKGDVISGLPQVEIEGEKVFHAGTENKGGDIVTNGGRVLCATALGNSVSEAQQRAYELAK
CCCCHHCCCCCEEECCCEEEECCCCCCCCCEEECCCEEEEEEHHCCCHHHHHHHHHHHHH
QIRWDGMFHRNDIGYRAIAREQQK
HHHHCCCEECCCCCHHHHHHCCCC
>Mature Secondary Structure 
PYQAPLFARFEDDEMNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLEN
CCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
VNIAVEDIAGLVAFAKEKAIELTIVGPEVPLVLGVVDAFYEAGLPIFGPTQAAAQLEGSK
CCEEHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCH
AFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAE
HHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHH
EAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGP
HHHHHHHCCCCCCCCCCEEEHHHHCCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCC
NTGGMGAYSPAPVVTPEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCEE
EYNCRFGDPETQPIMMRMESDLVELCLAAIDKKLDQVESKWDPRASIGIVLAAGGYPAAY
EEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
NKGDVISGLPQVEIEGEKVFHAGTENKGGDIVTNGGRVLCATALGNSVSEAQQRAYELAK
CCCCHHCCCCCEEECCCEEEECCCCCCCCCEEECCCEEEEEEHHCCCHHHHHHHHHHHHH
QIRWDGMFHRNDIGYRAIAREQQK
HHHHCCCEECCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]