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The map label for this gene is aspA

Identifier: 37681302

GI number: 37681302

Start: 3197756

End: 3199207

Strand: Direct

Name: aspA

Synonym: VV3118

Alternate gene names: 37681302

Gene position: 3197756-3199207 (Clockwise)

Preceding gene: 37681300

Following gene: 37681303

Centisome position: 95.33

GC content: 47.11

Gene sequence:

>1452_bases
ATGGCTACCCTAACTGAAGCTCCAAAAGCATCAACTCCGGCCACTCGTATTGAAGAAGATCTATTAGGCCAACGTCACGT
TCCGGCTGACGCTTACTACGGTATTCACACGCTGCGCGCCATCGAAAACTTCAACATCTCAAATGTCACTATTTCTGACG
TACCAGAATTCGTTCGTGGCATGGTTATGACCAAAAAAGCCGCTGCGCTTGCTAACAAAGAATTGGGCGCAATTCCTAAG
AATGTCGCGGATTACATCATTCAAGCGTGTGACCTAATGTTGGAAACTGGCAAATGCATGGATCAGTTCCCATCAGACGT
ATTCCAAGGCGGTGCGGGCACTTCTGTGAACATGAACACCAACGAAGTACTTGCCAACATTGCTCTAGAATTAATGGGCA
AAGAGAAAGGCGATTACGACGTGGTGAACCCAAACGATCACGTGAACAAGAGCCAATCAACCAACTGTGCCTACCCAACA
GGCTTCCGTATCGCGGTTTACAACAGCATCCATAAATTGATGGAAGCGATTGAATACCTAAAAGGTGCGTTTGAGCTGAA
ATCTCAAGAGTTTAACGACATCCTAAAAATGGGCCGTACTCAGTTGCAAGATGCAGTTCCAATGACTGTCGGTCAAGAGT
TCCACGCTTGGGCAGTGACACTAAACGAAGAGATCCGTGCGCTAGACTACACCTCGAAGCTACTTCTTGAAGTGAACCTA
GGTGCAACGGCAATCGGTACTGGCTTAAACACACCTCCTGGTTACCAAGCACTTGCCGTGAAACACCTAGCGGAAGTAAC
TGGCCTAGAAGTGGTTCCAGCAGAAGACCTTATCGAAGCAACTTCAGACTGTGGTGCTTACGTAATGGCACACGGCGCTC
TAAAACGCCTAGCGGTGAAATTGTCTAAGATCTGTAACGACTTGCGTCTGCTTTCTTCTGGTCCTCGCGCTGGTTTGAAC
GAACTTAACTTGCCAGAAATGCAAGCCGGTTCTTCAATCATGCCAGCAAAAGTTAACCCAGTTATCCCAGAAGTGGTTAA
CCAAGTGTGTTTCAAAGTACTGGGTAACGACAACACGATTTCTTTCGCAGCCGAAGGCGGTCAGCTACAGCTGAACGTAA
TGGAGCCTGTGATTGGTCAAGCGATGTTTGAATCGATTTCACTACTACAAAACGCGTGTGTGAACCTACGTGACAAGTGT
ATCGACGGCATCACTGTGAACAAAGAGATCTGTGAAAACTACGTTTACAACTCTATCGGTATCGTTACTTACCTAAACCC
ATACATCGGCCACCACGAAGGTGACATCGTTGGTAAGATCTGTGCGGAAACCGGTAAGAGCGTCCGTGAAGTAGTGCTTG
AGCGTGGCTTGCTGACATCTGAAGAGTTGGATGACATCCTGTCTGTGCAGAACTTCATGCACCCAACTTACAAAGCAAAA
CGTTACGAATAA

Upstream 100 bases:

>100_bases
GACGGATTTTACAGCCAAATACTCTGATGAATGGATTCTGATTGCAGAAAATGGCTAACCAATCACTTAAATATCAGAAT
TATTATCTAAGGATCCTGTT

Downstream 100 bases:

>100_bases
TCGTAAAGAGAAAGGGCTCCTTAGAGGGCCCTTTTTGCCGTTCTTTATTAATAACCATAATAATAACAAGTATTTATTTT
ACTTCTGATTATTCAAGCGC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRGMVMTKKAAALANKELGAIPK
NVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNTNEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPT
GFRIAVYNSIHKLMEAIEYLKGAFELKSQEFNDILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
GATAIGTGLNTPPGYQALAVKHLAEVTGLEVVPAEDLIEATSDCGAYVMAHGALKRLAVKLSKICNDLRLLSSGPRAGLN
ELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTISFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKC
IDGITVNKEICENYVYNSIGIVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
RYE

Sequences:

>Translated_483_residues
MATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRGMVMTKKAAALANKELGAIPK
NVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNTNEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPT
GFRIAVYNSIHKLMEAIEYLKGAFELKSQEFNDILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
GATAIGTGLNTPPGYQALAVKHLAEVTGLEVVPAEDLIEATSDCGAYVMAHGALKRLAVKLSKICNDLRLLSSGPRAGLN
ELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTISFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKC
IDGITVNKEICENYVYNSIGIVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
RYE
>Mature_482_residues
ATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRGMVMTKKAAALANKELGAIPKN
VADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNTNEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPTG
FRIAVYNSIHKLMEAIEYLKGAFELKSQEFNDILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNLG
ATAIGTGLNTPPGYQALAVKHLAEVTGLEVVPAEDLIEATSDCGAYVMAHGALKRLAVKLSKICNDLRLLSSGPRAGLNE
LNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTISFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKCI
DGITVNKEICENYVYNSIGIVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAKR
YE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=467, Percent_Identity=39.4004282655246, Blast_Score=331, Evalue=1e-90,
Organism=Escherichia coli, GI87082375, Length=467, Percent_Identity=79.4432548179872, Blast_Score=750, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=467, Percent_Identity=39.186295503212, Blast_Score=322, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17553882, Length=478, Percent_Identity=39.9581589958159, Blast_Score=347, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=39.5705521472393, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=40.5639913232104, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=40.4301075268817, Blast_Score=341, Evalue=8e-94,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=39.9132321041215, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=39.9132321041215, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=39.0928725701944, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24583245, Length=448, Percent_Identity=35.0446428571429, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52631; Mature: 52500

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRG
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MVMTKKAAALANKELGAIPKNVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNT
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCH
NEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPTGFRIAVYNSIHKLMEAIEYL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KGAFELKSQEFNDILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEECHHHHHHHHHCEEEEEECC
GATAIGTGLNTPPGYQALAVKHLAEVTGLEVVPAEDLIEATSDCGAYVMAHGALKRLAVK
CCEEECCCCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSKICNDLRLLSSGPRAGLNELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
SFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKCIDGITVNKEICENYVYNSIG
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
IVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
HHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
RYE
CCC
>Mature Secondary Structure 
ATLTEAPKASTPATRIEEDLLGQRHVPADAYYGIHTLRAIENFNISNVTISDVPEFVRG
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MVMTKKAAALANKELGAIPKNVADYIIQACDLMLETGKCMDQFPSDVFQGGAGTSVNMNT
HHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCH
NEVLANIALELMGKEKGDYDVVNPNDHVNKSQSTNCAYPTGFRIAVYNSIHKLMEAIEYL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KGAFELKSQEFNDILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALDYTSKLLLEVNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEEECHHHHHHHHHCEEEEEECC
GATAIGTGLNTPPGYQALAVKHLAEVTGLEVVPAEDLIEATSDCGAYVMAHGALKRLAVK
CCEEECCCCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHH
LSKICNDLRLLSSGPRAGLNELNLPEMQAGSSIMPAKVNPVIPEVVNQVCFKVLGNDNTI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
SFAAEGGQLQLNVMEPVIGQAMFESISLLQNACVNLRDKCIDGITVNKEICENYVYNSIG
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
IVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTSEELDDILSVQNFMHPTYKAK
HHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
RYE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]