| Definition | Vibrio vulnificus YJ016 chromosome I, complete sequence. |
|---|---|
| Accession | NC_005139 |
| Length | 3,354,505 |
Click here to switch to the map view.
The map label for this gene is mrcA [H]
Identifier: 37681179
GI number: 37681179
Start: 3070308
End: 3072821
Strand: Direct
Name: mrcA [H]
Synonym: VV2995
Alternate gene names: 37681179
Gene position: 3070308-3072821 (Clockwise)
Preceding gene: 37681164
Following gene: 37681181
Centisome position: 91.53
GC content: 48.41
Gene sequence:
>2514_bases GTGAAGTTCATAAAGCGTTTACTTATATTTGCATTAATTTGCATAATTCTTGGAGTCACAACAATTTTTGGCTTTTATCA GTACGTTAAGCCAGAACTGCCAGATGTGGCGACACTCAAAAATGTTGAGTTACAAACTCCGATGCAGGTGTTTAGCCAAG ATGGGAAATTAATTGCGCAATTTGGTGAAAAGCGTCGCATTCCAGTGACCTACGACCAAATTCCTCAATCGCTGATCCAC GCGCTGATCGCGACTGAAGATAGCCGTTTCTATGATCATTACGGTATCGACCCGATTGGTATTACCCGAGCGGCGTTAGT TGTCGCGATGTCAGGTTCCGCTAAACAGGGCGCAAGTACCATTACTCAGCAATTGGCACGTAACTTCTTCCTCTCGAATG AGAAGAAATTAATGCGTAAAGTGAAAGAAATTTTTATTGCCATCCATATTGAGCAACTGCTGAGCAAGCAGGAAATCATG GAGCTTTACGTTAATAAAATTTTCCTTGGTTATCGCTCATACGGTTTTGGGGCGGCAGCGCGCGTCTATTTTGGCAAAGA TTTGCAAGATCTCTCCTTAAGCGAGATTGCGACTTTGGCGGGTATGCCAAAAGCACCATCGACACTGAACCCTATCTATT CGCTAGAACGCGCAACCAATCGTCGTAACGTCGTCCTAATGCGCATGTTGGATGAAAAATACATCACCCAAGAAGAGTAT GACGCAGCACGAGCGGAAACATTGATTTCTCAATTCCACGGCGCGGAGATCGAGGTCAGTGCGCCTTATGTGGCTGAGCT CGCGCGTGCATGGATGGTACAGGAGTACGGCGAAGAGAAAGCGTATACGTCCGGTATGAATGTCTACATGACCATCGACT CTAAGCTCCAAGCCGCAGCCAACAAAGCCGCGATTGACAACTTGTTAGCCTACGATGAGCGTCACGGCTACCGTGGTGAA GAAAGTGTGCTGTGGAAAGCAGGCGAAGCCGCTTGGGATGAAGCGAAGATGGACGAGCATCTGAGCAACCAACCGACTTA CGGCGAATTGATTCCAGCGATTGTGACCAAGGTTGAAAGCAAAAACGCAACCATTTGGGTTAAACGTGAACAAAGCCAAA CCATCGCATGGTCAGGCATGAACTGGGCGCGCAAGTTCATCACTGATGAACGTCAAGGCAGTGCACCAAACTCAGCCAAA GACATCTTAGCGCCGGGCGAGCAAATTTGGGTTCGCAAAGTAGTGACTACCGATGACAAAGGCGAAAGCCAGGAAAGCTG GCACTTAAGTCAAGTGCCTAACGCCAATACCGCCTTTATCGCCATGAACCCTGATAATGGCGCGGTACTTTCCCTCGTGG GTGGCTTTAACTTTGTTCACAACAAGTTCAACCGTGCCACCATGTCGGTTCGCCAAGTAGGTTCGAGTATCAAACCCTTT ATTTACTCGGCGGCGATTGATAAAGGCATGACTCTGGCCACGTTGATCAACGATGCACCAATCAACAAATGGGATGAGAG CCAAGGTACAGCATGGCGTCCTAAGAACTCTCCTCCGACTTACATTGGCCCAACCCGTTTACGTATTGGCTTAGCAACGT CGAAAAACGTCATGGCGGTTCGTACTCTGCGTGAAGTCGGGCTGGACGAAACGCGTGAATACCTCACTCGCTTTGGTTTT GAACTCGACCAACTGCCACGCTCAGAAACCATTGCCTTGGGTGCAGGTAGCTTAACCCCAATGAAGATGGCACAGGGATT CTCGGTTTTTGCCAATGGGGGCTACTATGTAGAGCCTTATTACATCAGCCATATTGAAGATCCTTATGGCAATGTCGTGT TTGAGTCGCAGCCACAAACCATCTGTCAGCAAGATTGCCCAGTGGCCACGCAAGCCATTGTAGAAAATGAGAATGGTGAA AGCACCGAGAGCACTCAGCCATTTGCCAAGCAAGTGATCAGCGAACAGAATGCTTTTATCATGCGCGAAATGATGTACAG CAACATCTGGGGTGGTGGTAACTGGCGTGAAGGTACAGGTTGGAATGGCACTGGCTGGCGTGCACAAACGTTGGGTCGAC GCGATATCGGCGGTAAAACTGGTACAACCAACGATTCCAAAGACGCTTGGTATAACGGTTATGGCCCGGGTGTGGTCGCG ATTTCTTGGGTAGGCTTTGATGACCATAGTCGCACGCTCGGAAAAACCACCACTAACGCCAACTTAGATGGTAAACAAAC CTCTGGGGCCGAATCTGGAGCGAAAACTGCATTGCCAGCGTGGATTGATTTTATGAAACTGGCTTTAGAAGGCGTGCCTG AACATGAAAAAGTGTTACCCGCGAATATAGTGCGTGTGCGCATCGATCGTGAAACCGGGCTATTAACCAACAAGACCGAT GACAGCGCTATGTTTGAGTACTTCATTAAAGGGACAGAGCCGACTGAGTATGTTTCTAGCGTCGAGGAAGAAAGCATCTA TTCCTCTTCCAGTGATGAAGAAGCGCTGTTCTAA
Upstream 100 bases:
>100_bases ATTAACCGTCAAATAAGAGTTAATAGCAGCAGAGCTAACTTTTTAGCCTAGAGTACAAGGGTTTGCCGTCATACAGCCTC AACTAAACAGGGATTCTCCG
Downstream 100 bases:
>100_bases GTTAGCCAGTCAAACACAAAAAGGTTGCCATTTGGCAACCTTTTTATTTATTGGCGGAGCAGACAAACATTACTGCTCTG AACCGCTCACGAGCAGCTCT
Product: penicillin-binding protein 1A
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 837; Mature: 837
Protein sequence:
>837_residues MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQFGEKRRIPVTYDQIPQSLIH ALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGASTITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIM ELYVNKIFLGYRSYGFGAAARVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAANKAAIDNLLAYDERHGYRGE ESVLWKAGEAAWDEAKMDEHLSNQPTYGELIPAIVTKVESKNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAK DILAPGEQIWVRKVVTTDDKGESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF IYSAAIDKGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAVRTLREVGLDETREYLTRFGF ELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPYYISHIEDPYGNVVFESQPQTICQQDCPVATQAIVENENGE STESTQPFAKQVISEQNAFIMREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLPANIVRVRIDRETGLLTNKTD DSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF
Sequences:
>Translated_837_residues MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQFGEKRRIPVTYDQIPQSLIH ALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGASTITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIM ELYVNKIFLGYRSYGFGAAARVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAANKAAIDNLLAYDERHGYRGE ESVLWKAGEAAWDEAKMDEHLSNQPTYGELIPAIVTKVESKNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAK DILAPGEQIWVRKVVTTDDKGESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF IYSAAIDKGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAVRTLREVGLDETREYLTRFGF ELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPYYISHIEDPYGNVVFESQPQTICQQDCPVATQAIVENENGE STESTQPFAKQVISEQNAFIMREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLPANIVRVRIDRETGLLTNKTD DSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF >Mature_837_residues MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQFGEKRRIPVTYDQIPQSLIH ALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGASTITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIM ELYVNKIFLGYRSYGFGAAARVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAANKAAIDNLLAYDERHGYRGE ESVLWKAGEAAWDEAKMDEHLSNQPTYGELIPAIVTKVESKNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAK DILAPGEQIWVRKVVTTDDKGESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF IYSAAIDKGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAVRTLREVGLDETREYLTRFGF ELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPYYISHIEDPYGNVVFESQPQTICQQDCPVATQAIVENENGE STESTQPFAKQVISEQNAFIMREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLPANIVRVRIDRETGLLTNKTD DSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=849, Percent_Identity=52.5323910482921, Blast_Score=912, Evalue=0.0, Organism=Escherichia coli, GI1786343, Length=296, Percent_Identity=35.1351351351351, Blast_Score=152, Evalue=1e-37, Organism=Escherichia coli, GI1788867, Length=202, Percent_Identity=37.1287128712871, Blast_Score=123, Evalue=5e-29, Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=32.3863636363636, Blast_Score=81, Evalue=3e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.-
Molecular weight: Translated: 93280; Mature: 93280
Theoretical pI: Translated: 5.27; Mature: 5.27
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQ CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHCCCHHHHH FGEKRRIPVTYDQIPQSLIHALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGAST HCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHEEECCCCCCHHHH ITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIMELYVNKIFLGYRSYGFGAAA HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE RVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY EEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHCCHHHH DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAA HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEEECHHHHHHH NKAAIDNLLAYDERHGYRGEESVLWKAGEAAWDEAKMDEHLSNQPTYGELIPAIVTKVES HHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC KNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAKDILAPGEQIWVRKVVTTDDK CCCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHEEEEEEECCCC GESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF CCCCCCEEHHCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHH IYSAAIDKGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAV HHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCHHHH RTLREVGLDETREYLTRFGFELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPY HHHHHCCCHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCHHHHHCCCEEEECCCEEECCE YISHIEDPYGNVVFESQPQTICQQDCPVATQAIVENENGESTESTQPFAKQVISEQNAFI EEECCCCCCCCEEECCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHH MREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLP EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC ANIVRVRIDRETGLLTNKTDDSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF CEEEEEEEECCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC >Mature Secondary Structure MKFIKRLLIFALICIILGVTTIFGFYQYVKPELPDVATLKNVELQTPMQVFSQDGKLIAQ CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHCCCHHHHH FGEKRRIPVTYDQIPQSLIHALIATEDSRFYDHYGIDPIGITRAALVVAMSGSAKQGAST HCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHEEECCCCCCHHHH ITQQLARNFFLSNEKKLMRKVKEIFIAIHIEQLLSKQEIMELYVNKIFLGYRSYGFGAAA HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE RVYFGKDLQDLSLSEIATLAGMPKAPSTLNPIYSLERATNRRNVVLMRMLDEKYITQEEY EEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHCCHHHH DAARAETLISQFHGAEIEVSAPYVAELARAWMVQEYGEEKAYTSGMNVYMTIDSKLQAAA HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEEECHHHHHHH NKAAIDNLLAYDERHGYRGEESVLWKAGEAAWDEAKMDEHLSNQPTYGELIPAIVTKVES HHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC KNATIWVKREQSQTIAWSGMNWARKFITDERQGSAPNSAKDILAPGEQIWVRKVVTTDDK CCCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHEEEEEEECCCC GESQESWHLSQVPNANTAFIAMNPDNGAVLSLVGGFNFVHNKFNRATMSVRQVGSSIKPF CCCCCCEEHHCCCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHH IYSAAIDKGMTLATLINDAPINKWDESQGTAWRPKNSPPTYIGPTRLRIGLATSKNVMAV HHHHHHHCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCCHHHH RTLREVGLDETREYLTRFGFELDQLPRSETIALGAGSLTPMKMAQGFSVFANGGYYVEPY HHHHHCCCHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCHHHHHCCCEEEECCCEEECCE YISHIEDPYGNVVFESQPQTICQQDCPVATQAIVENENGESTESTQPFAKQVISEQNAFI EEECCCCCCCCEEECCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHH MREMMYSNIWGGGNWREGTGWNGTGWRAQTLGRRDIGGKTGTTNDSKDAWYNGYGPGVVA HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE ISWVGFDDHSRTLGKTTTNANLDGKQTSGAESGAKTALPAWIDFMKLALEGVPEHEKVLP EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCC ANIVRVRIDRETGLLTNKTDDSAMFEYFIKGTEPTEYVSSVEEESIYSSSSDEEALF CEEEEEEEECCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10952301 [H]