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The map label for this gene is pcnB [H]

Identifier: 37680945

GI number: 37680945

Start: 2814447

End: 2815838

Strand: Direct

Name: pcnB [H]

Synonym: VV2761

Alternate gene names: 37680945

Gene position: 2814447-2815838 (Clockwise)

Preceding gene: 37680944

Following gene: 37680946

Centisome position: 83.9

GC content: 49.21

Gene sequence:

>1392_bases
GTGATGACCACTCACTGTTGCCAGATGCACATGAATAAAAACGAATATACAACATGCGATTCCACCACGTGTCCGGAACT
CGCTTTAAATGTCATTACTCGTCAAGAGCACAATATTTCGCGCAAGCAGATTAGCGACCATGCGCTAAAAGTACTGTACC
GACTTCATGGCGCCGGCTTCGACGCTTTTCTTGTTGGCGGTGGCGTACGTGATTTACTGCTGGGACAAAGCCCAAAAGAT
TTTGATATTGCAACCAACGCGACACCTGAACAGCTAAAGCAGTTGTTCCGTAACTGCCGCCTGATTGGTCGCCGCTTTCG
ACTAGCGCATATCATGTTTGGTCGCGAAATCATTGAAGTGGCCACGTTCCGTGGACACCACCAAGATGCCAATTCGCAGA
TCTCCCAGCAGTCAAAAGAAGGCATGCTGTTGCGTGATAACGTGTATGGCACCATTGATGAAGATGCTGAACGACGTGAC
TTCACCATCAATGCGATGTACTACAACATTGCAGATTACTCGATCCACGATTATGCAGGTGGTGTTGAAGACCTTGAAGA
TCGCTTAGTGCGCTTAATTGGCGATCCTGAGACTCGCTATCGTGAAGATCCTGTACGTATGCTTCGCGCGATTCGATTTG
CCGCCAAACTCGACTTCGATATCGAAGAAGACACTGCCGCACCGATCGAAGAGATGGCGCACTTGTTAAGAGACATCCCT
GCCGCGCGTTTATACGAAGAATCGCTTAAGCTACTGCAATCGGGCCATGGTTTGGAAACGTATCACCTCATGCGTGAATA
CAATCTATTCCAACAAATGTTCCCAGCGATCTCAGCTTATTTCACCGAGGATTACTCTTCCAAAGCTGAGCAGATGTTGG
ACTTAGTCCTAGACTCCACAGACATTCGTGTCGAAGAAGGCAAGCGCATTAATCCCGCTTACATGTTTGCGGCTATGCTG
TGGTATCCGCTGCTTGAGCTGGCAGACAAATTGATGGATAGCCACAATTTGTGTCACTACGACGCCGTCATGGAAGCGAG
CAACCTGATCCTTGATGAACAGGTGCGCTCGATAGCTATTCCGCGCCGCCACACCGCGACCATCCGTGAAATTTGGCAAC
TGCAACTGCGTCTACCTCGCCGCAACGGTAAACGTGCTTTCCGCCTAATGGAATTGAACAAATTCCGTGCTGGTTTTGAC
TTCCTAGAAATGCGAGGAGAGATCGAAGGCGGTGAAACGGCAACATTGGCCAAGTGGTGGGGCACGTTCCAAAACGCAGG
TCGTGAAATGCGCCTAGCGATGGTAAACGATCTCGAAGGCCCTGCTGGCAGTTCAAAATCCGGCCGTAAGCGTCGTTCTT
TTAATAAGAAAAAGAGCAAAGCCAAATCATGA

Upstream 100 bases:

>100_bases
AATCGCTAGAAATCACGGCGCGATTCTCAAATGGCACGGCGTGAGCTATTATAGGGCGCAAATTCAGGCCAAATCGGCGT
AAAAAACTAACCCATGCAAA

Downstream 100 bases:

>100_bases
TTACTGCCTATATTGCCATTGGCAGTAATCTTGCCGATCCTGTTAGCCAAGCCAAGAGCGCCATTAATGCACTAAAAACG
CTGCCAAAATCGCGCCTACT

Product: polyA polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MMTTHCCQMHMNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGFDAFLVGGGVRDLLLGQSPKD
FDIATNATPEQLKQLFRNCRLIGRRFRLAHIMFGREIIEVATFRGHHQDANSQISQQSKEGMLLRDNVYGTIDEDAERRD
FTINAMYYNIADYSIHDYAGGVEDLEDRLVRLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAHLLRDIP
AARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDSTDIRVEEGKRINPAYMFAAML
WYPLLELADKLMDSHNLCHYDAVMEASNLILDEQVRSIAIPRRHTATIREIWQLQLRLPRRNGKRAFRLMELNKFRAGFD
FLEMRGEIEGGETATLAKWWGTFQNAGREMRLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS

Sequences:

>Translated_463_residues
MMTTHCCQMHMNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGFDAFLVGGGVRDLLLGQSPKD
FDIATNATPEQLKQLFRNCRLIGRRFRLAHIMFGREIIEVATFRGHHQDANSQISQQSKEGMLLRDNVYGTIDEDAERRD
FTINAMYYNIADYSIHDYAGGVEDLEDRLVRLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAHLLRDIP
AARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDSTDIRVEEGKRINPAYMFAAML
WYPLLELADKLMDSHNLCHYDAVMEASNLILDEQVRSIAIPRRHTATIREIWQLQLRLPRRNGKRAFRLMELNKFRAGFD
FLEMRGEIEGGETATLAKWWGTFQNAGREMRLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS
>Mature_463_residues
MMTTHCCQMHMNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGFDAFLVGGGVRDLLLGQSPKD
FDIATNATPEQLKQLFRNCRLIGRRFRLAHIMFGREIIEVATFRGHHQDANSQISQQSKEGMLLRDNVYGTIDEDAERRD
FTINAMYYNIADYSIHDYAGGVEDLEDRLVRLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAHLLRDIP
AARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDSTDIRVEEGKRINPAYMFAAML
WYPLLELADKLMDSHNLCHYDAVMEASNLILDEQVRSIAIPRRHTATIREIWQLQLRLPRRNGKRAFRLMELNKFRAGFD
FLEMRGEIEGGETATLAKWWGTFQNAGREMRLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=220, Percent_Identity=32.7272727272727, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI87081691, Length=436, Percent_Identity=56.8807339449541, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789436, Length=238, Percent_Identity=28.9915966386555, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=188, Percent_Identity=29.2553191489362, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=292, Percent_Identity=27.7397260273973, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357337, Length=292, Percent_Identity=27.7397260273973, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 53421; Mature: 53421

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTHCCQMHMNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGF
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
DAFLVGGGVRDLLLGQSPKDFDIATNATPEQLKQLFRNCRLIGRRFRLAHIMFGREIIEV
CEEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFRGHHQDANSQISQQSKEGMLLRDNVYGTIDEDAERRDFTINAMYYNIADYSIHDYAG
HHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHCC
GVEDLEDRLVRLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAHLLRDIP
CHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
AARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDST
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIRVEEGKRINPAYMFAAMLWYPLLELADKLMDSHNLCHYDAVMEASNLILDEQVRSIAI
CCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
PRRHTATIREIWQLQLRLPRRNGKRAFRLMELNKFRAGFDFLEMRGEIEGGETATLAKWW
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHH
GTFQNAGREMRLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS
HHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMTTHCCQMHMNKNEYTTCDSTTCPELALNVITRQEHNISRKQISDHALKVLYRLHGAGF
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
DAFLVGGGVRDLLLGQSPKDFDIATNATPEQLKQLFRNCRLIGRRFRLAHIMFGREIIEV
CEEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFRGHHQDANSQISQQSKEGMLLRDNVYGTIDEDAERRDFTINAMYYNIADYSIHDYAG
HHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHCC
GVEDLEDRLVRLIGDPETRYREDPVRMLRAIRFAAKLDFDIEEDTAAPIEEMAHLLRDIP
CHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
AARLYEESLKLLQSGHGLETYHLMREYNLFQQMFPAISAYFTEDYSSKAEQMLDLVLDST
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DIRVEEGKRINPAYMFAAMLWYPLLELADKLMDSHNLCHYDAVMEASNLILDEQVRSIAI
CCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
PRRHTATIREIWQLQLRLPRRNGKRAFRLMELNKFRAGFDFLEMRGEIEGGETATLAKWW
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHH
GTFQNAGREMRLAMVNDLEGPAGSSKSGRKRRSFNKKKSKAKS
HHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]