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The map label for this gene is rlmL

Identifier: 37679840

GI number: 37679840

Start: 1710385

End: 1712508

Strand: Direct

Name: rlmL

Synonym: VV1656

Alternate gene names: 37679840

Gene position: 1710385-1712508 (Clockwise)

Preceding gene: 37679839

Following gene: 37679841

Centisome position: 50.99

GC content: 45.1

Gene sequence:

>2124_bases
ATGAACCAATATCTTGCGGTTACCTCAAATGGTCTTGAGAACCTTCTTGCTGAAGAACTAACAAAACTTGGAATTGCAGA
CGCTAAGCCAGTACAGGCTGGAGTAAGATTTAAAGCCTCTAATGAACAGGTTTATCGTTGCTGTCTATGGAGTAGGTTGG
CATCGCGTTTTGTTCGCGTGTTGTCCGAGTTTACCTGTCAAAGTGATATGGACTTGTATTTGGCAACGTCCTCTATCAAT
TGGGTAAATCAATTCCACAGCACTAAGAAGTTTGTGGTTGATTTCAACGGCACGAACCGTGAGATTCGTAACAGCCAGTA
CGGTGCGATGAAAGTGAAAGATGGCATCGTAGATTGTTTTGAAAAGAAAAACCTGCCACGTCCAAATATCAGCAAAGAAC
GTCCGGATGTACGTATCCATGTTCGTCTTCACAAAGACAAAGCGATTCTTGGTGTTGATATGGTCGGGAATGGCTTGCAC
CAACGTGGCTATCGTCCTGCTTCTGGTAAGGCACCACTCCGTGAAACCTTGGCTGCTGCCATCATCCTTCGCTCAGGTTG
GACAGAGGAACAACCTCTTCTGGATCCAATGTGTGGCTCAGGTACGTTGTTGATAGAGGCGGCCATGATGGCAGCAAACA
TGGCGCCTGGCGTAAAACGTGAAAAGTGGGTCTTTGAGTCACTTGAAGATTTCGAGCCAGAAGTGTGGGCAGAAATAAAA
TCAGAAGCAAATGTCATTGCCCGAAGAGGTGTCAAAAAAGTTGGCGCAAGATTCTATGGCTTTGATAATGATGAAAAAGT
ACTGAAAATTGCCCAAGATAACGCTCGCAGAGCAGGGGTTGCGGATCTTATCGAGTTTAAGTTAGGTGACGCCGCTACAG
TGTCACGTCCTCAAGAATTTGAGAATGGTGTAATTGTTTGTAACCCACCTTATGGTGAGCGACTTGGCACAGAGCCAGGA
TTGATTGCACTATACACAGCGTTTGGTGGCCAGTTAAAAGCCCAGTTTGGTGGGTGTACGGCATCTATTTTCTCGAGTTC
AGATGAGTTGTTGAGCTGTCTTCGCATGCGTGCGGAAAAACAGTTTAAATTGAATAACGGTGCGTTACCTTGTCACCAAA
AAAACTATACGATCGCACAAAGAAGTGCAGAGCAGATTGAAGGTGCGGATGGCCCGCTTTCTATCGCACCAGACTTTGCT
AACCGCCTAAAGAAAAATATCGGTAAGATTGGCAAATGGGCTCACAAAGAACAGCTCGATTGTTACCGCATCTATGATGC
CGACCTGCCAGAATACAATGTGGCGATAGACGTCTATCAAGATCACTTGGTGATTCAAGAATACGCCGCGCCGAAAAACA
TTCCAGAAGAGAAGGCAAAGCGTCGCCTGACGGATATCATTCGTGCTGCGATTCAGGTAACTGGCGTAGAAGCCAACAAA
GTTGTGCTGAAAGTTCGCGAGAAACAAAAAGGTCGTAACCAATATCAAAAATTGGGTCAAGTTTCTGAGCGAATGGAAGT
TAACGAGTATGGTGCAAAGCTGATCGTCAATTTGTACGATTATCTGGATACGGGGTTGTTCCTAGATCATAAGATCACGC
GCCGTCGTTTGGGCGAAATGGCAAAAGGAGCCGATTTCCTTAACCTGTTTGCTTATACAGGGAGTGCCACGGTACACGCC
GCTCTTGGCGGTGCCAGCTCGACAACAACGGTTGACATGTCGAACACCTACCTTGAGTGGGCAAAAGACAACATGTCTGC
AAATGGTTGTATCGGACGTCAACATAAGTTTGAGCAGGCAGATTGTTTACAGTGGTTAGAGAAAGCACAGGGACAGTACG
ATTTAATCTTTATTGATCCGCCGACGTTTTCAAACTCTAAGCGTATGGAAGACTCGTTTGACGTTCAGCGCGACCATATC
AAACTGATGAAAAACTTAAAACGTTTGCTAAGAGAAAATGGCACGATTGTCTTCTCTAACAATAAACGTCATTTCAAAAT
GGATATGGAAGCGATGGAAGAGTTGGGTTTAGACGCGAAGAACATTTCTTCTCAGACACTGCCACTCGATTTCTCAAGAA
ACAAACATATTCATAACTGTTGGGTCGTCACCCACAAAAAATAG

Upstream 100 bases:

>100_bases
ACACTGGCTCATAGGGAGATGAGAAAAGTCCTTGAAATGCGAGGCATGGAGTAGATTTGTCAGGTATAATCAACGCCATT
TTTAATAGCTAAAGAACACT

Downstream 100 bases:

>100_bases
GAACAAGGAACGTGATCACCCTATACAGTACCGAAGGATGCCATCTGTGTGAGATGGCCTACGAGTTGGTGGAACAAGCA
GGCATTGCTGAACAAGTTCA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRVLSEFTCQSDMDLYLATSSIN
WVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCFEKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLH
QRGYRPASGKAPLRETLAAAIILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEFENGVIVCNPPYGERLGTEPG
LIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEKQFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFA
NRLKKNIGKIGKWAHKEQLDCYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEMAKGADFLNLFAYTGSATVHA
ALGGASSTTTVDMSNTYLEWAKDNMSANGCIGRQHKFEQADCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHI
KLMKNLKRLLRENGTIVFSNNKRHFKMDMEAMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK

Sequences:

>Translated_707_residues
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRVLSEFTCQSDMDLYLATSSIN
WVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCFEKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLH
QRGYRPASGKAPLRETLAAAIILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEFENGVIVCNPPYGERLGTEPG
LIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEKQFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFA
NRLKKNIGKIGKWAHKEQLDCYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEMAKGADFLNLFAYTGSATVHA
ALGGASSTTTVDMSNTYLEWAKDNMSANGCIGRQHKFEQADCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHI
KLMKNLKRLLRENGTIVFSNNKRHFKMDMEAMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK
>Mature_707_residues
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRVLSEFTCQSDMDLYLATSSIN
WVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCFEKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLH
QRGYRPASGKAPLRETLAAAIILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEFENGVIVCNPPYGERLGTEPG
LIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEKQFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFA
NRLKKNIGKIGKWAHKEQLDCYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEMAKGADFLNLFAYTGSATVHA
ALGGASSTTTVDMSNTYLEWAKDNMSANGCIGRQHKFEQADCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHI
KLMKNLKRLLRENGTIVFSNNKRHFKMDMEAMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=705, Percent_Identity=56.8794326241135, Blast_Score=835, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=264, Percent_Identity=26.8939393939394, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_VIBVY (Q7MKX1)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_934449.1
- ProteinModelPortal:   Q7MKX1
- SMR:   Q7MKX1
- STRING:   Q7MKX1
- GeneID:   2624462
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV1656
- NMPDR:   fig|196600.1.peg.1724
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   VVUL196600:VV1656-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 79626; Mature: 79626

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRV
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
LSEFTCQSDMDLYLATSSINWVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCF
HHHHCCCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCCHHCCCCCCCEEEHHHHHHHH
EKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLHQRGYRPASGKAPLRETLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEHHHCCCHHHCCCCCCCCCCHHHHHHHHH
IILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEF
HHHHHHHHHHHHHHCHHHCCCCCCHHHEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCHHH
ENGVIVCNPPYGERLGTEPGLIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEK
CCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEHHHHCCCHHHHHHHHHHHHH
QFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFANRLKKNIGKIGKWAHKEQLD
CEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHCHHHHCCCC
CYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
EEEEECCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHH
AKGADFLNLFAYTGSATVHAALGGASSTTTVDMSNTYLEWAKDNMSANGCIGRQHKFEQA
HCCCHHHHHEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCHH
DCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHIKLMKNLKRLLRENGTIVFSN
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
NKRHFKMDMEAMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK
CCCEEEECHHHHHHHCCCHHCCCCCCCCEECCCCCCEEEEEEEEECC
>Mature Secondary Structure
MNQYLAVTSNGLENLLAEELTKLGIADAKPVQAGVRFKASNEQVYRCCLWSRLASRFVRV
CCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
LSEFTCQSDMDLYLATSSINWVNQFHSTKKFVVDFNGTNREIRNSQYGAMKVKDGIVDCF
HHHHCCCCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCCHHCCCCCCCEEEHHHHHHHH
EKKNLPRPNISKERPDVRIHVRLHKDKAILGVDMVGNGLHQRGYRPASGKAPLRETLAAA
HHCCCCCCCCCCCCCCEEEEEEEECCCEEEEEHHHCCCHHHCCCCCCCCCCHHHHHHHHH
IILRSGWTEEQPLLDPMCGSGTLLIEAAMMAANMAPGVKREKWVFESLEDFEPEVWAEIK
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
SEANVIARRGVKKVGARFYGFDNDEKVLKIAQDNARRAGVADLIEFKLGDAATVSRPQEF
HHHHHHHHHHHHHHCHHHCCCCCCHHHEEEHHCCCCCCCHHHHHHHCCCCCCCCCCCHHH
ENGVIVCNPPYGERLGTEPGLIALYTAFGGQLKAQFGGCTASIFSSSDELLSCLRMRAEK
CCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEHHHHCCCHHHHHHHHHHHHH
QFKLNNGALPCHQKNYTIAQRSAEQIEGADGPLSIAPDFANRLKKNIGKIGKWAHKEQLD
CEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHCHHHHCCCC
CYRIYDADLPEYNVAIDVYQDHLVIQEYAAPKNIPEEKAKRRLTDIIRAAIQVTGVEANK
EEEEECCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
VVLKVREKQKGRNQYQKLGQVSERMEVNEYGAKLIVNLYDYLDTGLFLDHKITRRRLGEM
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHH
AKGADFLNLFAYTGSATVHAALGGASSTTTVDMSNTYLEWAKDNMSANGCIGRQHKFEQA
HCCCHHHHHEEECCCCEEEEEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCHH
DCLQWLEKAQGQYDLIFIDPPTFSNSKRMEDSFDVQRDHIKLMKNLKRLLRENGTIVFSN
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
NKRHFKMDMEAMEELGLDAKNISSQTLPLDFSRNKHIHNCWVVTHKK
CCCEEEECHHHHHHHCCCHHCCCCCCCCEECCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA