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The map label for this gene is citS [H]

Identifier: 37679787

GI number: 37679787

Start: 1647835

End: 1649454

Strand: Direct

Name: citS [H]

Synonym: VV1603

Alternate gene names: 37679787

Gene position: 1647835-1649454 (Clockwise)

Preceding gene: 37679781

Following gene: 37679788

Centisome position: 49.12

GC content: 48.64

Gene sequence:

>1620_bases
ATGACGTGGCAAAGCATCAGTTTTCGTAAAAGAATGCTGGTCATTATGACGCTCTCCGGATTAATTGAGTTGCTTATCCT
CGTAGCAGCGGGCTTTGCCTACCTCAAATACTCGCAAGAGCAAGAAATGGGGCAGAAGGCACTCGGTGTCGCGCGATTTC
TTGCAACCTCTACGACGGTTATCGAGTTGATCGAAAATCGTGACAATGTCACCTATCAGCAAAAATTTCGAGAGTTAACC
CAAGCATTGAGTGCAGCCTTCATTGTGGTAGGTGATCGTGAAGGTATTCGGTTGATCCACCCCGTTGATGAACGTCTTGG
TTTGCCGATGCGTGGTGGTGATAACGCCCGAGCATTGGAAGAAGGCGAAGCGTATATCTCGACAGCAAGAGGTTCACTGG
GCTATTCGGTGCGTGGCAAAGCAGCGGTTTTTAACTCGGCGGGTGAAGTGATCGGTGTGGTTTCTGTGGGTTATCTGCTG
GATCGATTACAAGACAGAATAGAGCCATTTGCGTGGTTTTTAATTACTATGACGTTACTGGTGGTTGCTGCGAATGCCTT
GGTTTCCAATTACGCTTCTCGTAAATTTCAGCGAGCGATTTTGGGGTTTGAACCGGAAGAAATTGGTCGGTTATATGGTG
AGCTTGATGTGACCATGAGCACCATTAAAGAAGGGGTGTTAAGTATTGATTCGAGTGGCGTGCTGCGTTCCATCAACCGC
AGTGCAGCGCAGATTCTCGGGTTAGATCCGGCCCAAGTGCTGAACAAACCGCTCTCTGATGCGTTGCCCGCCAGTGATCT
GTACCGTGTATTAGAAACGGGACAAGAAGATCACGATATTGAACTTTACCTCAACCAAAAGAGGTTGATTGCCAACCGAA
GCCCGGTTTACGTAGAGGGAAAGATTGTCGGCGCGGTGTCCAGTTTTCGTTTGCGTGATGAAATTTCTGAGCTCACTGAA
CAGCTTTCTCAAACCAAAGAGTATGCCGATTTATTACGCTCACAAACGCATGAACATCGCAACAAACTTAATACCATCAG
TGGCCTAGTTCAGATGGGCGAGCTTGATGCGGTTCAACAGCTTATCGGGCAGGAGACGGCGCACTATCAGGGGTTGATCG
AGTTTCTACGCGATACCATCAAAGATCCGTTGGTCGCGGGCATGTTGCTAGGAAAAACGGAACGCGCGCGTGAGCTTGGC
TTAGAGTTAGTGGTTGAAGAAGGGGCTCGTTTAGAGCCGCTAAGTGCTTGGCTGAACCCAGAAGACATCACGACAATACT
CGGTAACCTGATTGATAACGCTTTTGACGCGACCATGAGCGCGATCGCGCAAGAAGGCAATTTTGCCCGTTCACGTAGAA
CCATTGAAGTTTCCATCAGTGACTACGGAACGGAAGTGATTCTTGAAGTGCAGGATCAAGGTTGTGGTTTGCCCAAACAA
TTTTCCACCGAGCAGCTGCTTGAGAAAGGGATCTCGAGCAAAGCGACCAGCACTCGGGGTGTTGGGCTCTACTTAGTCAA
TCAATTAGCCGCACGTTACGGCGGCTCGATAGAGATGGCTGAGAATGACAAATTTGGCACAAGAATGACGGTCTATTTAC
CCAAGGAAGAACAAAGATGA

Upstream 100 bases:

>100_bases
TTGCGATTCACACTTCCGGAGGGTGGTAAGAGCTTGCATGTTAAAACGGTGTTAAGTTAACTGAGTTTTCGATGTTTTTA
AGTGGTGCAAAGAGGTAAGC

Downstream 100 bases:

>100_bases
ATGCCGATACCCGAGTGATGATAATTGAAGATGATATGGCGATTGCTCAGCTTCATCATAAGTATTTGCAGCAAATGGAT
GGTTTCGAGGTGATTGGCAT

Product: putative sensor kinase CitA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTVIELIENRDNVTYQQKFRELT
QALSAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALEEGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLL
DRLQDRIEPFAWFLITMTLLVVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEGKIVGAVSSFRLRDEISELTE
QLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQLIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELG
LELVVEEGARLEPLSAWLNPEDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYGGSIEMAENDKFGTRMTVYLPKEEQR

Sequences:

>Translated_539_residues
MTWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTVIELIENRDNVTYQQKFRELT
QALSAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALEEGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLL
DRLQDRIEPFAWFLITMTLLVVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEGKIVGAVSSFRLRDEISELTE
QLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQLIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELG
LELVVEEGARLEPLSAWLNPEDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYGGSIEMAENDKFGTRMTVYLPKEEQR
>Mature_538_residues
TWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTVIELIENRDNVTYQQKFRELTQ
ALSAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALEEGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLLD
RLQDRIEPFAWFLITMTLLVVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINRS
AAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEGKIVGAVSSFRLRDEISELTEQ
LSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQLIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELGL
ELVVEEGARLEPLSAWLNPEDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQF
STEQLLEKGISSKATSTRGVGLYLVNQLAARYGGSIEMAENDKFGTRMTVYLPKEEQR

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=541, Percent_Identity=33.826247689464, Blast_Score=326, Evalue=3e-90,
Organism=Escherichia coli, GI1790567, Length=546, Percent_Identity=30.03663003663, Blast_Score=208, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59619; Mature: 59488

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IELIENRDNVTYQQKFRELTQALSAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALE
HHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEECCCHHHCCCCCCCCCCCCHHH
EGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLLDRLQDRIEPFAWFLITMTLL
HCHHHHHHHCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEG
HHHHHHCCCHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCEEEEE
KIVGAVSSFRLRDEISELTEQLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQ
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELGLELVVEEGARLEPLSAWLNP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHEECCCCCCCHHHHCCCH
EDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCEEEEEECCCCCCCCCC
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYGGSIEMAENDKFGTRMTVYLPKEEQR
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TWQSISFRKRMLVIMTLSGLIELLILVAAGFAYLKYSQEQEMGQKALGVARFLATSTTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IELIENRDNVTYQQKFRELTQALSAAFIVVGDREGIRLIHPVDERLGLPMRGGDNARALE
HHHHHCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCEEECCCHHHCCCCCCCCCCCCHHH
EGEAYISTARGSLGYSVRGKAAVFNSAGEVIGVVSVGYLLDRLQDRIEPFAWFLITMTLL
HCHHHHHHHCCCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAANALVSNYASRKFQRAILGFEPEEIGRLYGELDVTMSTIKEGVLSIDSSGVLRSINR
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SAAQILGLDPAQVLNKPLSDALPASDLYRVLETGQEDHDIELYLNQKRLIANRSPVYVEG
HHHHHHCCCHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHCCCCCEEEEE
KIVGAVSSFRLRDEISELTEQLSQTKEYADLLRSQTHEHRNKLNTISGLVQMGELDAVQQ
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGQETAHYQGLIEFLRDTIKDPLVAGMLLGKTERARELGLELVVEEGARLEPLSAWLNP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHEECCCCCCCHHHHCCCH
EDITTILGNLIDNAFDATMSAIAQEGNFARSRRTIEVSISDYGTEVILEVQDQGCGLPKQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEECCCCCEEEEEECCCCCCCCCC
FSTEQLLEKGISSKATSTRGVGLYLVNQLAARYGGSIEMAENDKFGTRMTVYLPKEEQR
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]