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The map label for this gene is rluB [H]

Identifier: 37679411

GI number: 37679411

Start: 1238593

End: 1239555

Strand: Direct

Name: rluB [H]

Synonym: VV1227

Alternate gene names: 37679411

Gene position: 1238593-1239555 (Clockwise)

Preceding gene: 37679409

Following gene: 37679414

Centisome position: 36.92

GC content: 49.53

Gene sequence:

>963_bases
ATGAACGAAAAATTACAGAAAGTTTTGGCACGAGCTGGTCATGGCTCACGTCGTGAATTGGAAGCATTAATCAAATCAGG
ACGTGTGAGTGTGAACGGTATCGTCGCGAAACTGGGCGAACGACTAGAAGATGAGCAAGCAGTAGTGCGCATCGATGGTC
ACGTTGTGTCCGTGAAAGCTCAGGAAGAGGTGATTTGCCGAGTGTTGGCATACTACAAACCTGAAGGTGAGCTCTGTACG
CGTCACGACCCAGAAGGTCGCCGTACTGTTTTTGATCGTCTACCAAAAATCCGTGGCTCTCGCTGGATCTCTGTTGGTCG
TCTTGATGCAAACACCTCTGGTTTGTTGCTTTTTACCACTGATGGTGAATTGGCAAATCGCCTAATGCACCCAAGTCGTC
AAGTTGAACGTGAATACCTAGTTCGCGTGTTTGGCGAAGTGAATGAGCACAAGGTACGTAACCTCGTGAACGGCGTTGAG
CTCGATGATGGTTTAGCTCGCTTTGAAGACGTGGTTTATACTGGCGGTGAAGGTATGAACCATACGTTTTACGTGGTGAT
CAATGAAGGTCGTAACCGTGAGGTTCGTCGTCTTTGGGAATCTCAGGATTGCACAGTAAGCCGCTTAAAACGTGTACGTT
ATGGCGACATTTATCTTGATAAGAAACTGCCACGTGGCGGTTGGATGGAGCTTGAGCTGAAAGACGTGAACTACCTACGC
GAGTTGGTAGAGCTTCGTCCAGAGAAAGAGACCATGATTGAGTTGAATAACTCATCACGTAAGCGTGACCGTGCCCGTAG
TCAGAAGATTCGCCGCGCGGTAAAACGTCATGAAGAGCGCGTGAGTACGCCAAAAGGTCGCAGCAACAATGCGGCACGAC
GTAAGCCAAGTAAGGCCGCGGGTCAAACAAGCAAAACCAATAGCCGCAACCAAGCGGGTAGCCGCTCTAAGCCACAGCGC
TAA

Upstream 100 bases:

>100_bases
TATAATCGTCGCCCCGATTCCGAGCATCGGGTAGACATTGTAAACGGGTCTAGCTTGGTAAGACTACGACGTCTGAGAAG
ACGACAATTTAGGTAGATAA

Downstream 100 bases:

>100_bases
GAGAACGAGCAGAAATAAAAAGGCTTGCCATTGGCAAGCCTTTTTTGCTTCTTAATCTCGTTTCCAGAGAATGTGACAGA
ACTTTTGGTCTGGATCGCGG

Product: pseudouridine synthase family 1 protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVE
LDDGLARFEDVVYTGGEGMNHTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKAAGQTSKTNSRNQAGSRSKPQR

Sequences:

>Translated_320_residues
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVE
LDDGLARFEDVVYTGGEGMNHTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKAAGQTSKTNSRNQAGSRSKPQR
>Mature_320_residues
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKAQEEVICRVLAYYKPEGELCT
RHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTTDGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVE
LDDGLARFEDVVYTGGEGMNHTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKAAGQTSKTNSRNQAGSRSKPQR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=295, Percent_Identity=64.4067796610169, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=28.6307053941909, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=29.5652173913043, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 36764; Mature: 36764

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKA
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEC
QEEVICRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVELDDGLARFEDVVYTGGEGMN
CHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCC
HTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
EEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHHHH
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKAA
HHHHHCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHC
GQTSKTNSRNQAGSRSKPQR
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNEKLQKVLARAGHGSRRELEALIKSGRVSVNGIVAKLGERLEDEQAVVRIDGHVVSVKA
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCEEEEEEC
QEEVICRVLAYYKPEGELCTRHDPEGRRTVFDRLPKIRGSRWISVGRLDANTSGLLLFTT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEC
DGELANRLMHPSRQVEREYLVRVFGEVNEHKVRNLVNGVELDDGLARFEDVVYTGGEGMN
CHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCC
HTFYVVINEGRNREVRRLWESQDCTVSRLKRVRYGDIYLDKKLPRGGWMELELKDVNYLR
EEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHHHH
ELVELRPEKETMIELNNSSRKRDRARSQKIRRAVKRHEERVSTPKGRSNNAARRKPSKAA
HHHHHCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHC
GQTSKTNSRNQAGSRSKPQR
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA