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The map label for this gene is hisS

Identifier: 37678951

GI number: 37678951

Start: 771516

End: 772784

Strand: Direct

Name: hisS

Synonym: VV0767

Alternate gene names: 37678951

Gene position: 771516-772784 (Clockwise)

Preceding gene: 37678950

Following gene: 37678952

Centisome position: 23.0

GC content: 48.31

Gene sequence:

>1269_bases
GTGGCAAAAACAATCCAAGCAATTCGAGGCATGAATGATTGTCTCCCAACTCAATCACCACTGTGGCAGAAACTAGAAGG
CGCAGTGAAGAACGTAATTAGCGCATACGGTTACAATGAAATGCGCATGCCAATCGTTGAGATGACGCATCTATTTAGCC
GTGCTATTGGCGAAGTGACCGATGTGGTTGAAAAAGAGATGTACACCTTTGAAGACCGTAATGGTGATAGCTTAACATTA
CGTCCAGAAGGCACCGCGGGTTGTGTGCGTGCCGGGATTGAAAACGGTTTGTTGTACAACCAAGAACAGCGCGTTTGGTA
CATGGGTCCTATGTTCCGCCACGAGCGTCCACAAAAAGGTCGTTACCGTCAATTCCACCAGTGTGGTGTAGAAGTTTTTG
GTCTCAATGGCCCTGACGTCGATGCAGAGCTGATCATGATGACTGCGCGTCTTTGGCGTGAACTTGGCATTGATAAACAT
GTGCGTCTAGAGCTGAACTCGATTGGTTCGTTGGAAGCGCGCGCAAACTATCGCACTGCACTGATCGCATTTCTTGAGCA
ACATATCGATGTGCTGGATGAAGATTGCAAACGTCGTATGCATACTAACCCACTACGTGTATTGGATACCAAGAATCCAG
ATGTACAAGCGATTTTGGGTGATGCGCCACGTTTGTCTGACTACTTAGATCCAGAGTCAACGCAACATTTTGCGGGTTTG
TGTGAACTTCTTGACGCGGCGGGTATCGAATACACAGTCAACGAACGCTTAGTTCGTGGCCTGGATTACTACAACCGCAC
GGTTTTTGAATGGATCACGGAAAGCCTAGGCTCTCAAGGTACCGTTTGTGGTGGTGGCCGTTACGATGGCCTAGTCGAGC
AACTTGGTGGCAAAGCTACACCAGCGGTGGGTTTTGCAATGGGCTTAGAGCGTTTAGTGCTAATGTTGGAAACGTTAGAG
CTGACGGATGTTCGTCGTAGTGTGGACGTTTACGTCGTCACTGCGGGTGAAGGCACTATGATGGCTGGCATGAAGTTGGC
GGAACAAGTTCGTGAAGCCATTCCTGGGGTTCGCGTGATGAACCACTTTGGTGGTGGTAACTTTAAGAAACAATTTAAAC
GTGCTGACAAAGTGGGCGCGGTTGTTGCCCTAGTACTGGGTGAGAATGAAGTTGCAGACAACACCGTTGTATTGAAAGAT
CTCGCTGGTGGTGAGCAAGTTACGTATTCACAGCAAGAGATTGCCAGCAAAATTGCTGAGCTGATTTAA

Upstream 100 bases:

>100_bases
AGATCCGTGCCAAAGCGGCACGCATGGATGAAAGCAACCGCATCGACATTAAAGTGCAAAACTAAATCTCAACGAAGTCA
TTCAAATTACGGTAATTATT

Downstream 100 bases:

>100_bases
GCTCTGGCAGCAGTCACAACAATGGCAGCGGTTTCGCTGCCATTGAATCAGTTTTAAAGAGGACAGGAAGTGGAAGTTTA
CAATACTGAAGAAGAACAAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MAKTIQAIRGMNDCLPTQSPLWQKLEGAVKNVISAYGYNEMRMPIVEMTHLFSRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRAGIENGLLYNQEQRVWYMGPMFRHERPQKGRYRQFHQCGVEVFGLNGPDVDAELIMMTARLWRELGIDKH
VRLELNSIGSLEARANYRTALIAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSDYLDPESTQHFAGL
CELLDAAGIEYTVNERLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKATPAVGFAMGLERLVLMLETLE
LTDVRRSVDVYVVTAGEGTMMAGMKLAEQVREAIPGVRVMNHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKD
LAGGEQVTYSQQEIASKIAELI

Sequences:

>Translated_422_residues
MAKTIQAIRGMNDCLPTQSPLWQKLEGAVKNVISAYGYNEMRMPIVEMTHLFSRAIGEVTDVVEKEMYTFEDRNGDSLTL
RPEGTAGCVRAGIENGLLYNQEQRVWYMGPMFRHERPQKGRYRQFHQCGVEVFGLNGPDVDAELIMMTARLWRELGIDKH
VRLELNSIGSLEARANYRTALIAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSDYLDPESTQHFAGL
CELLDAAGIEYTVNERLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKATPAVGFAMGLERLVLMLETLE
LTDVRRSVDVYVVTAGEGTMMAGMKLAEQVREAIPGVRVMNHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKD
LAGGEQVTYSQQEIASKIAELI
>Mature_421_residues
AKTIQAIRGMNDCLPTQSPLWQKLEGAVKNVISAYGYNEMRMPIVEMTHLFSRAIGEVTDVVEKEMYTFEDRNGDSLTLR
PEGTAGCVRAGIENGLLYNQEQRVWYMGPMFRHERPQKGRYRQFHQCGVEVFGLNGPDVDAELIMMTARLWRELGIDKHV
RLELNSIGSLEARANYRTALIAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSDYLDPESTQHFAGLC
ELLDAAGIEYTVNERLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKATPAVGFAMGLERLVLMLETLEL
TDVRRSVDVYVVTAGEGTMMAGMKLAEQVREAIPGVRVMNHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKDL
AGGEQVTYSQQEIASKIAELI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=459, Percent_Identity=23.3115468409586, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI6996014, Length=470, Percent_Identity=22.7659574468085, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=72.8132387706856, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71993686, Length=470, Percent_Identity=25.9574468085106, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71993693, Length=470, Percent_Identity=25.9574468085106, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325290, Length=481, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24643061, Length=480, Percent_Identity=24.5833333333333, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24643059, Length=495, Percent_Identity=24.6464646464646, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_VIBVU (Q8DEZ9)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759422.2
- ProteinModelPortal:   Q8DEZ9
- SMR:   Q8DEZ9
- GeneID:   1177381
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0426
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   VVUL216895:VV1_0426-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47042; Mature: 46910

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIQAIRGMNDCLPTQSPLWQKLEGAVKNVISAYGYNEMRMPIVEMTHLFSRAIGEVT
CCCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIENGLLYNQEQRVWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQCGVEVFGLNGPDVDAELIMMTARLWRELGIDKHVRLELNSIGSLEARANYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
LIAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSDYLDPESTQHFAGL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCHHHCCCCCCHHHHHHH
CELLDAAGIEYTVNERLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKAT
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLMLETLELTDVRRSVDVYVVTAGEGTMMAGMKLAEQVREAIPGVRVM
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHH
NHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKDLAGGEQVTYSQQEIASKIAE
HHCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHH
LI
HC
>Mature Secondary Structure 
AKTIQAIRGMNDCLPTQSPLWQKLEGAVKNVISAYGYNEMRMPIVEMTHLFSRAIGEVT
CCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIENGLLYNQEQRVWYMGPMFRHERPQKG
HHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQCGVEVFGLNGPDVDAELIMMTARLWRELGIDKHVRLELNSIGSLEARANYRTA
HHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
LIAFLEQHIDVLDEDCKRRMHTNPLRVLDTKNPDVQAILGDAPRLSDYLDPESTQHFAGL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCHHHCCCCCCHHHHHHH
CELLDAAGIEYTVNERLVRGLDYYNRTVFEWITESLGSQGTVCGGGRYDGLVEQLGGKAT
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLMLETLELTDVRRSVDVYVVTAGEGTMMAGMKLAEQVREAIPGVRVM
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHH
NHFGGGNFKKQFKRADKVGAVVALVLGENEVADNTVVLKDLAGGEQVTYSQQEIASKIAE
HHCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCEECCHHHHHHHHHH
LI
HC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA