Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 37678808

GI number: 37678808

Start: 611941

End: 613080

Strand: Direct

Name: carA [H]

Synonym: VV0624

Alternate gene names: 37678808

Gene position: 611941-613080 (Clockwise)

Preceding gene: 37678807

Following gene: 37678809

Centisome position: 18.24

GC content: 47.54

Gene sequence:

>1140_bases
TTGAGTAAATCAGCACTGTTAGTCCTGGAAGATGGGACAGTGTTCCGCGGAGTGTCCATTGGCGCAGATGGTATTTCCGT
TGGAGAAGTCGTTTTCAATACCTCGATGACGGGGTATCAAGAAATCCTCACTGATCCTTCTTATTCCCAGCAAATCGTTA
CTCTTACTTACCCTCACATTGGCAATACCGGAACCACTTCCGAAGACGAAGAATCCTCTTCCATTCATGCTCAAGGCCTT
GTGATTCGCGATCTCCCTCTTATTGCTTCCAACTTCCGTAGTGAACAATCTCTTTCTGATTACCTCAAATCGCAAAACAT
TGTCGGCATTGCTGATATTGATACGCGTAAGCTCACTCGTATTCTGCGTGAGAAAGGTGCACAAAACGGTTGTATCGTTG
CAGGTAACAACCTAGATGAAGCTTTGGCTCTGGCGAAAGCAAAAGAATTCCCAGGTTTAAAAGGAATGGATCTTGCGAAA
GAGGTTACAACAAAAGAAGCGTATCAATGGAAACAAGGTTCGTGGACGCTTGAGGGTGGACTTCCAGAAGCGAAAGATGA
CAGCGAATTGCCATATCACGTTGTTGCCTACGATTTCGGCGCAAAACGCAACATCTTACGAATGTTGGTTGACCGCGGCT
GCCGCTTAACGGTCGTGCCAGCTGAAACGTCGGCAGAAGACGTTCTAGCGCTAAACCCAGATGGTGTTTTCCTGTCAAAC
GGCCCTGGTGACCCAGCACCTTGTACTTATGCGATTGAAGCAACCAAAGTGTTCCTAGAAAAAGGCCTACCAGTATTTGG
TATCTGTCTTGGCCACCAAATTCTCGCGTTGGCGTCTGGTGCGAAAACGGTGAAGATGAAGTTTGGTCACCACGGTGCAA
ACCATCCTGTAAAAGATTTAGACCGCAATGTGGTGATGATTACTTCGCAAAACCACGGTTTTGCCGCTGATGAAGCAACT
CTACCAGATAACCTACGTGCTACTCACGTATCGCTCTTTGATGGCTCTTTGCAGGGAATTCACCGCACAGACAAGCCAGC
ATTCAGCTTCCAAGGTCACCCAGAAGCGAGCCCAGGTCCACACGACGCAGCTCCGCTATTTGACCATTTCATCGAACTGA
TTAAACAACACAGCGCTTAA

Upstream 100 bases:

>100_bases
CAATTTGTCTGAATTCCCCAAAATCACAATTTTCTGGTGATAGATAGGGCAGATTTGCAGTTTAATTTATTTTTTATGCA
TTTTTATTCTGGAGGTTGTC

Downstream 100 bases:

>100_bases
TTTGGAGTAGTAGACAATGCCAAAACGTACTGACATTCAAAGCATTCTTATCCTAGGTGCTGGTCCAATTGTTATCGGTC
AGGCTTGTGAGTTTGACTAC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTTSEDEESSSIHAQGL
VIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAK
EVTTKEAYQWKQGSWTLEGGLPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
GPGDPAPCTYAIEATKVFLEKGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRNVVMITSQNHGFAADEAT
LPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQHSA

Sequences:

>Translated_379_residues
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTTSEDEESSSIHAQGL
VIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAK
EVTTKEAYQWKQGSWTLEGGLPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
GPGDPAPCTYAIEATKVFLEKGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRNVVMITSQNHGFAADEAT
LPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQHSA
>Mature_378_residues
SKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTTSEDEESSSIHAQGLV
IRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAKE
VTTKEAYQWKQGSWTLEGGLPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSNG
PGDPAPCTYAIEATKVFLEKGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRNVVMITSQNHGFAADEATL
PDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQHSA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=386, Percent_Identity=39.8963730569948, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI169790915, Length=391, Percent_Identity=36.8286445012788, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI21361331, Length=391, Percent_Identity=36.8286445012788, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=82.4933687002653, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=385, Percent_Identity=36.6233766233766, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324878, Length=391, Percent_Identity=39.386189258312, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=37.0843989769821, Blast_Score=225, Evalue=9e-60,
Organism=Drosophila melanogaster, GI45555749, Length=394, Percent_Identity=40.1015228426396, Blast_Score=240, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=40.1015228426396, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40831; Mature: 40699

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCCEEEEEECCCEEEEEEECCCCCCHHHEEEECCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTTSEDEESSSIHAQGLVIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAKEVTTKEAYQWKQGSWTLEGG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEEECC
LPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEC
GPGDPAPCTYAIEATKVFLEKGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDL
CCCCCCCEEEEHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHC
DRNVVMITSQNHGFAADEATLPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGP
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HDAAPLFDHFIELIKQHSA
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCEEEEEECCCEEEEEEECCCCCCHHHEEEECCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTTSEDEESSSIHAQGLVIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAKEVTTKEAYQWKQGSWTLEGG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEEECC
LPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEC
GPGDPAPCTYAIEATKVFLEKGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDL
CCCCCCCEEEEHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHC
DRNVVMITSQNHGFAADEATLPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGP
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HDAAPLFDHFIELIKQHSA
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA