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The map label for this gene is epsD [H]

Identifier: 37678398

GI number: 37678398

Start: 225186

End: 227204

Strand: Direct

Name: epsD [H]

Synonym: VV0214

Alternate gene names: 37678398

Gene position: 225186-227204 (Clockwise)

Preceding gene: 37678397

Following gene: 37678399

Centisome position: 6.71

GC content: 48.39

Gene sequence:

>2019_bases
GTGAAACATTGGTTTAGCAAAAGCGCTTGGCTACTCGCAGGTACCTTAGCCTGCTCAAGCGGCGTGCTGGCAAACGAGTT
TAGTGCAAGCTTTAAAGGCACCGATATTCAGGAGTTCATCAATATTGTCGGTCGAAACTTAGAAAAGACCATCATTGTCG
ATCCTTCGGTGCGAGGTAAAATTGATGTACGCAGCTACGACGTATTAAATGAAGAGCAGTACTACAGCTTTTTCCTAAAC
GTACTTGAAGTGTATGGCTATGCGGTGGTTGAAATGGAAAATGGCGTACTCAAAGTCGTCAAATCCAAAGACTCCAAAAC
CTCCGCCATTCCTGTGGTCAGTGATGACACCGTGAAGGGCGACAATGTCATTACACGCGTGGTTGCGGTGCGCAATGTCT
CTGTGCGAGAGCTGTCTCCTTTGCTACGCCAACTGATTGATAATGCGGGCGCTGGTAACGTGGTGCACTATGATCCGGCC
AATATCATCTTGATCACGGGTCGTGCAGCAGTGGTGAATCGTTTGGCAGAAATCATCAAACGCGTAGACCAAGCGGGTGA
CACCGAAATCGAAGTGGTAGAGCTAGGTAATGCGTCGGCGGCGGAAATGGTCCGTATTGTTGATGCGCTAAACCGCACTA
CCGATGCGAAAAACACCCCAGAATTTTTGCAGCCAAAATTGGTGGCCGATGAGCGCACCAACTCGATTTTGATTTCGGGT
GATCCCAAAGTGCGTGATCGCTTGAAGCGTTTGATTCGCCAACTCGATGTGGAAATGGCGAGCAAAGGCAACAACCGTGT
TGTTTACCTCAAATACGCCAAAGCGGAAGATTTGGTTGATGTACTTAAAGGGGTCTCTGATAACCTGCAAGCAGAGAAAA
ACTCTGGTCAAAAGGGCGCGTCTTCACAGCGCAACGATGTGGTGATCGCCGCGCATCAAGGCACCAACTCACTGGTATTG
ACCGCGCCGCCCGATATTATGTTGGCGCTGCAAGATGTGATTACCCAGTTAGATATTCGCCGTGCACAAGTGCTGATTGA
AGCCTTGATTGTCGAAATGGCCGAGGGCGATGGTGTGAACTTGGGTGTGCAATGGGGTAACTTGGAAACGGGTGCTGTCA
TCCAATATAGCAATACCGGTACGCCAATTGGCAAAGTAATGGTTGGCTTGGAAGAGGCAAAAGATCAAACTAAAACTGAA
TATTACACCAATAAGGATGGGGATCGAGTCCCTTACCAAGTTACTGAGTCTGGAGACTATTCCACCCTTGCGGCAGCGCT
TGCTGGCGTAAACGGTGCGGCAATGAGCCTTGTTATGGGGGACTGGACAGCCCTGATCAGCGCAGTTTCGAGTGATTCCA
ACTCCAATATCCTGTCTTCGCCCAGCATCACCGTAATGGACAATGGTGAAGCGTCTTTCATTGTTGGCGAAGAAGTTCCT
GTTATTACCGGTTCAACCGCGGGCTCGAACAACGATAACCCGTTCCAAACCGTCGATCGTAAAGAAGTGGGCATCAAGCT
TAAAGTGGTACCACAAATTAACGAAGGCGACTCGGTTCAGCTGAATATCGAACAAGAAGTATCTAACGTTTTGGGTGCTA
ATGGGGCGGTGGACGTTCGTTTTGCTAAACGTCAGCTCAATACCTCAGTGATTGTGCAAGATGGCCAAATGTTGGTGCTT
GGTGGTTTGATCGATGAGCGTGCGTTAGAGAGTGAATCAAAAGTACCGCTACTGGGCGATATTCCAATTTTGGGGCATTT
GTTTAAATCCACCAATACTCAAGTAGAAAAGAAAAACCTAATGGTGTTCATCAAGCCGACCATCATTCGCGATGGCATGA
CGGCGGATGGTATTACCCAGCGTAAGTACAACTACATTCGCGCCGAGCAGCTCTACAAAGCGGATCAGGGCCTTAAATTG
ATGGATGATGGCCACATTCCTGTGTTGCCGAAATTTGGTGAAGACAAACGCCATCCGGCTGAAATTCAAGCCTTTATCGA
TCAAATGGAACAGCAGTAA

Upstream 100 bases:

>100_bases
CACCGAGTTGAACCTCACGGTGGAACGAGACGGACAGCAGCATGATATTTACATCCAGTTTTAACACTTAGGCTCAAAGC
GTAAGTTCAGGGAGATAAAC

Downstream 100 bases:

>100_bases
TGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGAA
ATCGAGCACCCAGAAAGGCC

Product: type II secretory pathway, component EpsD

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKIDVRSYDVLNEEQYYSFFLN
VLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKGDNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPA
NIILITGRAAVVNRLAEIIKRVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGASSQRNDVVIAAHQGTNSLVL
TAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVNLGVQWGNLETGAVIQYSNTGTPIGKVMVGLEEAKDQTKTE
YYTNKDGDRVPYQVTESGDYSTLAAALAGVNGAAMSLVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEVP
VITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVIVQDGQMLVL
GGLIDERALESESKVPLLGDIPILGHLFKSTNTQVEKKNLMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLKL
MDDGHIPVLPKFGEDKRHPAEIQAFIDQMEQQ

Sequences:

>Translated_672_residues
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKIDVRSYDVLNEEQYYSFFLN
VLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKGDNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPA
NIILITGRAAVVNRLAEIIKRVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGASSQRNDVVIAAHQGTNSLVL
TAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVNLGVQWGNLETGAVIQYSNTGTPIGKVMVGLEEAKDQTKTE
YYTNKDGDRVPYQVTESGDYSTLAAALAGVNGAAMSLVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEVP
VITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVIVQDGQMLVL
GGLIDERALESESKVPLLGDIPILGHLFKSTNTQVEKKNLMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLKL
MDDGHIPVLPKFGEDKRHPAEIQAFIDQMEQQ
>Mature_672_residues
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKIDVRSYDVLNEEQYYSFFLN
VLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKGDNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPA
NIILITGRAAVVNRLAEIIKRVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGASSQRNDVVIAAHQGTNSLVL
TAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVNLGVQWGNLETGAVIQYSNTGTPIGKVMVGLEEAKDQTKTE
YYTNKDGDRVPYQVTESGDYSTLAAALAGVNGAAMSLVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEVP
VITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVIVQDGQMLVL
GGLIDERALESESKVPLLGDIPILGHLFKSTNTQVEKKNLMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLKL
MDDGHIPVLPKFGEDKRHPAEIQAFIDQMEQQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=668, Percent_Identity=44.7604790419162, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1789793, Length=349, Percent_Identity=25.5014326647564, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 73140; Mature: 73140

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGK
CCCCCCCCCCEEEEHHHHCCCCCHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
IDVRSYDVLNEEQYYSFFLNVLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKG
EEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCC
DNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEIIK
CHHHEEEHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCEEEEEC
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGA
CHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
SSQRNDVVIAAHQGTNSLVLTAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVN
CCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LGVQWGNLETGAVIQYSNTGTPIGKVMVGLEEAKDQTKTEYYTNKDGDRVPYQVTESGDY
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCH
STLAAALAGVNGAAMSLVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEVP
HHHHHHHHCCCHHHHEEHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCEEEEECCCCC
VITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVR
EEECCCCCCCCCCCCCCCCCHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHCCCCCEEEE
FAKRQLNTSVIVQDGQMLVLGGLIDERALESESKVPLLGDIPILGHLFKSTNTQVEKKNL
EHHHHCCCEEEEECCCEEEEECCHHHHHHCCCCCCCEEECCHHHHHHHHCCCCEEECCCE
MVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLKLMDDGHIPVLPKFGEDKRHPA
EEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
EIQAFIDQMEQQ
HHHHHHHHHHCC
>Mature Secondary Structure
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGK
CCCCCCCCCCEEEEHHHHCCCCCHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
IDVRSYDVLNEEQYYSFFLNVLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKG
EEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCC
DNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEIIK
CHHHEEEHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCEEEEEC
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGA
CHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
SSQRNDVVIAAHQGTNSLVLTAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVN
CCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LGVQWGNLETGAVIQYSNTGTPIGKVMVGLEEAKDQTKTEYYTNKDGDRVPYQVTESGDY
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCH
STLAAALAGVNGAAMSLVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEVP
HHHHHHHHCCCHHHHEEHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCEEEEECCCCC
VITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVR
EEECCCCCCCCCCCCCCCCCHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHCCCCCEEEE
FAKRQLNTSVIVQDGQMLVLGGLIDERALESESKVPLLGDIPILGHLFKSTNTQVEKKNL
EHHHHCCCEEEEECCCEEEEECCHHHHHHCCCCCCCEEECCHHHHHHHHCCCCEEECCCE
MVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLKLMDDGHIPVLPKFGEDKRHPA
EEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHH
EIQAFIDQMEQQ
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]