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The map label for this gene is hemB [H]

Identifier: 37678369

GI number: 37678369

Start: 194211

End: 195254

Strand: Direct

Name: hemB [H]

Synonym: VV0185

Alternate gene names: 37678369

Gene position: 194211-195254 (Clockwise)

Preceding gene: 37678366

Following gene: 37678370

Centisome position: 5.79

GC content: 49.23

Gene sequence:

>1044_bases
GTGTCTGTATCTATTCAAGGCCCATTCCCAGCGCGCCGTATGCGCCGTATGCGCAAGCATGAGTTCAGTCGTCGTTTGAT
GGCGGAGAATCAGCTATCGGTTAGCGACTTGATCTACCCGATGTTTATCTTAATGGGTAAAGATCGTCGTGAGAGTGTTG
AATCTATGCCTGGCGTAGAGCGCCTTTCCATTGACTTGATGCTTGAAGAAGCACAGTACCTTGCTCAGCTTGGTGTGCCA
GCGATTGCTCTTTTCCCTGTGGTGAATCAAGACGCGAAAAGCCTTTGTGCCGCGGAAGCTTATAATCCTGAAGGCTTGGT
TCAGAGAGCAGTGCGTGCATTAAAAGAGCACGTGCCAGCAATGGGCGTCATTACAGACGTTGCACTTGATCCTTTTACCA
CCCATGGTCAAGACGGCATCATTGATGAAGAAGGCTATGTGATGAATGATGAGACAACGGAAGTTCTGATCAAACAAGCG
TTGTCGCATGCGGAAGCGGGTGCGGATGTAGTGGCCCCCTCAGATATGATGGATGGTCGAATTGGCCGTATCCGTGACGC
TCTAGAGGAAGCGGGTTACATTCATACTCAAATCATGGCTTACTCCGCCAAATACGCATCCAACTACTACGGGCCATTCC
GTGATGCGGTGGGCTCTGCATCCAACCTAAAAGGTGGAAACAAAAAGAACTACCAAATGGATCCCGCCAACAGCGATGAA
GCGTTGCACGAAGTAGCATTAGACATTAATGAAGGTGCCGATATGGTGATGGTGAAGCCGGGCATGCCATACCTCGATGT
TGTACGCCGTGTTAAAACAGAGCTTCAAGTACCGACATTCGCCTATCAAGTGTCGGGCGAATATGCGATGCACAAAGCCG
CGATTCAAAATGGTTGGTTGAAAGAGCGTGAAACGGTGTTGGAATCGTTGCTTTGCTTTAAAAGAGCGGGTGCGGATGGC
ATTTTGACTTACTTTGCCAAAGATGTCGCACAATGGTTAGCGGAAGACAATGCGCAAGCGGCGCAATTCTTGAAAACCGA
ATAG

Upstream 100 bases:

>100_bases
GCATTTGGTCATTTTTGCGATTTTTCTTTATTGTTGCTGGCGCGCAGCCTTCGTAGTGATATGATTTTGCCAATTCTGTA
CACCAGACAAGGAGACGAGA

Downstream 100 bases:

>100_bases
TGGTAATCCCTCCTGCGAGGTTATGAGGCTGGCTTGTGCCAGCCTTTTTACGTTTTGTCAGGAAGATAACAATCAAAAAA
GCGCTTTGCTTGATGAGTAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSVSIQGPFPARRMRRMRKHEFSRRLMAENQLSVSDLIYPMFILMGKDRRESVESMPGVERLSIDLMLEEAQYLAQLGVP
AIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPAMGVITDVALDPFTTHGQDGIIDEEGYVMNDETTEVLIKQA
LSHAEAGADVVAPSDMMDGRIGRIRDALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
ALHEVALDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIQNGWLKERETVLESLLCFKRAGADG
ILTYFAKDVAQWLAEDNAQAAQFLKTE

Sequences:

>Translated_347_residues
MSVSIQGPFPARRMRRMRKHEFSRRLMAENQLSVSDLIYPMFILMGKDRRESVESMPGVERLSIDLMLEEAQYLAQLGVP
AIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPAMGVITDVALDPFTTHGQDGIIDEEGYVMNDETTEVLIKQA
LSHAEAGADVVAPSDMMDGRIGRIRDALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
ALHEVALDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIQNGWLKERETVLESLLCFKRAGADG
ILTYFAKDVAQWLAEDNAQAAQFLKTE
>Mature_346_residues
SVSIQGPFPARRMRRMRKHEFSRRLMAENQLSVSDLIYPMFILMGKDRRESVESMPGVERLSIDLMLEEAQYLAQLGVPA
IALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPAMGVITDVALDPFTTHGQDGIIDEEGYVMNDETTEVLIKQAL
SHAEAGADVVAPSDMMDGRIGRIRDALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSASNLKGGNKKNYQMDPANSDEA
LHEVALDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIQNGWLKERETVLESLLCFKRAGADGI
LTYFAKDVAQWLAEDNAQAAQFLKTE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=311, Percent_Identity=40.5144694533762, Blast_Score=228, Evalue=9e-60,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=45, Blast_Score=248, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6321398, Length=322, Percent_Identity=36.0248447204969, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21358291, Length=316, Percent_Identity=37.6582278481013, Blast_Score=217, Evalue=9e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38489; Mature: 38358

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSIQGPFPARRMRRMRKHEFSRRLMAENQLSVSDLIYPMFILMGKDRRESVESMPGVE
CCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHH
RLSIDLMLEEAQYLAQLGVPAIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPA
HHHHHHHHHHHHHHHHHCCCCEEHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
MGVITDVALDPFTTHGQDGIIDEEGYVMNDETTEVLIKQALSHAEAGADVVAPSDMMDGR
HHHHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCH
IGRIRDALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
ALHEVALDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIQNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCH
KERETVLESLLCFKRAGADGILTYFAKDVAQWLAEDNAQAAQFLKTE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
SVSIQGPFPARRMRRMRKHEFSRRLMAENQLSVSDLIYPMFILMGKDRRESVESMPGVE
CEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHH
RLSIDLMLEEAQYLAQLGVPAIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPA
HHHHHHHHHHHHHHHHHCCCCEEHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
MGVITDVALDPFTTHGQDGIIDEEGYVMNDETTEVLIKQALSHAEAGADVVAPSDMMDGR
HHHHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCH
IGRIRDALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
ALHEVALDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIQNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCH
KERETVLESLLCFKRAGADGILTYFAKDVAQWLAEDNAQAAQFLKTE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]